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EN1 Gene

protein-coding   GIFtS: 60

GC02M119315
engrailed homeobox 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
Hu-En-1 3
OTTHUMP00000162091 2
Descriptions
engrailed homeobox 1 2
engrailed homolog 1 2
External Ids
HGNC: 33421
Entrez Gene: 20192
UniProtKB: Q059253
Ensembl: ENSG000001630647
Search outside databases for aliases for EN1 gene

Previous GC identifers: GC02M116805 GC02M117520 GC02M119506 GC02M119694

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for EN1:
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the
'engrailed' (en) gene plays an important role during development in segmentation, where
it is required for the formation of posterior compartments. Different mutations in the mouse
homologs, En1 and En2, produced different developmental defects that frequently are lethal. The
human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated
in the control of pattern formation during development of the central nervous system. [provided by
RefSeq]

Gene Wiki entry for EN1
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the EN1 gene  

Entrez Gene cytogenetic band: 2q13-q21   Ensembl cytogenetic band:  2q14.2   HGNC cytogenetic band: 2q14.2

EN1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M119315:     (about GC identifiers)

Start:
119,316,217 bp from pter
End:
119,322,229 bp from pter
Size:
6,013 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000002.10  NT_022135.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: HME1_HUMAN, Q05925 (See protein sequence)
Recommended Name: Homeobox protein engrailed-1  
Size: 392 amino acids; 40115 Da
Subcellular location: Nucleus
Secondary accessions: Q4ZG44

REFSEQ proteins: NP_001417.3  

ENSEMBL proteins: 
ENSP00000295206 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (EN1 / Engrailed 1)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
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Antibodies for EN1: 
Browse Antibodies Central at Invitrogen
Millipore Mono- and Polyclonal Antibodies for the study of EN1
Sigma-Aldrich Antibodies for EN1
Browse R&D Systems for Antibodies
Antibodies from Abcam (EN1 / Engrailed 1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (EN1)
Novus Biologicals Antibodies for EN1

Assays for EN1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

5/7 InterPro domains/families (see all 7 ):
 IPR019737 Homoebox-engrailed_CS
 IPR015703 Homoebox-engrailed
 IPR019549 Homeobox-engrailed_C-terminal
 IPR001356 Homeobox
 IPR000747 Homeobox-engrailed


   GeneDecks  EN1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q05925

ProtoNet protein and cluster: Q05925

2 Blocks protein families:
IPB000047 Lambda and other repressor helix-turn-helix signature
IPB000747 'Homeobox' engrailed-type protein


UniProtKB/Swiss-Prot: HME1_HUMAN, Q05925
Similarity: Belongs to the engrailed homeobox family
Similarity: Contains 1 homeobox DNA-binding domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EN1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EN1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001426

              Applied Biosystems Silencer® siRNAs for EN1

              Sigma-Aldrich siRNA for EN1  
                     Sigma-Aldrich shRNA for EN1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001426
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001426
                                 untagged cDNA clone in CMV expression vector: NM_001426 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001426

Genatlas biochemistry entry for EN1:
Drosophila segment-polarity gene engrailed homolog 1,homeo domain encoding gene,expressed in
embryonic ventral limb ectoderm,regulator of the ventral limb patterning,inhibits WNT7A expression
in ventral limb bud

15/17 MGI mutant phenotypes (inferred from 15 alleles(MGI details for En1) (see all 17 ):

behavior/neurologicalcellularembryogenesisendocrine/exocrine gland
growth/sizehomeostasis/metabolismlethality-postnatallethality-prenatal/perinatallife span-post-weaning/aging
limbs/digits/tailnervous systemnormalpigmentationreproductive system

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003700 transcription factor activity IEA--
GO:0043565 sequence-specific DNA binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


 Gene Network CentralTM Interacting Genes and Proteins Network for  EN1 


5/15 Interacting proteins for EN1 (ENSP000002952063) via UniProtKB, MINT, and/or STRING (see all 15 )
InteractantInteraction Details
GeneCardExternal ID(s)
FGF8ENSP000003217973STRING (score=.99)
WNT1ENSP000002935493STRING (score=.982)
WNT7AENSP000002850183STRING (score=.952)
GPM6AENSP000002801873STRING (score=.915)
DKK1ENSP000003630813STRING (score=.906)
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5/12 Gene Ontology (GO) biological process terms (links to tree view) (see all 12 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0001501 skeletal system development TAS8684466
GO:0006355 regulation of transcription, DNA-dependent IEA--
GO:0007275 multicellular organismal development IEA--
GO:0009653 anatomical structure morphogenesis TAS8094370
GO:0009953 dorsal/ventral pattern formation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for EN1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EN1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EN1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001426

              Sigma-Aldrich siRNA for EN1  
                     Sigma-Aldrich shRNA for EN1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001426  

REFSEQ mRNAs for EN1 gene: 

NM_001426.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001426  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001426
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001426
                                 untagged cDNA clone in CMV expression vector: NM_001426 

2 DOTS entries:

DT.441697  DT.92415194 

6 AceView cDNA sequences:

AI924297 AI287694 AI283135 NM_001426 AA853350 BU617408 

highest scoring ESTs for EN1:

AA452915 AA853349 AA853350 AI283135 NM_001426 AI287694 AI924297 AA453083 BU617408 BX116136 

Unigene Cluster for EN1:

Engrailed homeobox 1
Hs.271977  [show with all ESTs]
Unigene Representative Sequence: NM_001426


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000295206  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

EN1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for EN1

1 / 2 / 3

3 probe-sets matching EN1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

43284_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI283135 0.60 1.00 0.82 1

220559_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001426 0.60 1.00 0.82 1

220559_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.271977
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for EN1 gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
EN11   -- engrailed homeobox 1 98.86(n)
99.38(a)
749965  XM_001172516.1  XP_001172516.1 
rat
(Rattus norvegicus)
En11   -- engrailed homeobox 1 88.75(n)
94.83(a)
685360  XM_001056699.1  XP_001056699.1 
mouse
(Mus musculus)
En11, 5 1 (64.10 cM)5
engrailed 11, 5 88.66(n)1
95.36(a)1
137981  NM_010133.11  NP_034263.11 
 AK1404085  AK1406345  (see all 11)
zebrafish
(Danio rerio)
zgc:1134991   -- zgc:113499 62.57(n)
67.86(a)
541371  NM_001013498.1  NP_001013516.1 
fruit fly
(Drosophila melanogaster)
en3
inv1
2 47F17--183
neuroblast determination specific RNA
polymerase more3
invected1
70(a)
(best of 2)3
49.47(n)1
42.69(a)1
362391  NM_165839.21  NP_725057.21 
About this table        Species with no ortholog for EN1

ENSEMBL Gene Tree for EN1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for EN1 gene
MEOX12  MEOX22  EN22  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/40 NCBI SNPs in EN1 are shown (see all 40 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 34)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 2 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs8935741,2
A,C,F,H119322855(-) GCTTTT/CATTTA 1 -- ng3113Minor allele frequency- C:0.07EU EA WA NA 2544
rs8935721,2
C,F,H119323940(-) CTTCCG/TCAGCC 1 -- ng3110Minor allele frequency- T:0.20NA EA EU WA 780
rs111234961,2
A,C119322056(+) GCGTCC/GGGTCC 1 -- ut51 ese30--------
rs38064991,2
F,H119322727(-) TGCGTT/GGCCCG 1 -- ng315Minor allele frequency- G:0.14EA EU WA 1884
rs37316131,2
C,F119322230(-) TCCCGG/TAGCTC 1 -- ng311Minor allele frequency- T:0.05EA 1490
--
rs49896491,2
C119321773(+) GGCTGG/Tttttt 1 -- ut51 trp30--------
--
rs37548571,2
C119321787(-) AAAAAA/CAAAAA 1 -- ut51 trp30--------
rs130059381,2
A119316205(+) AGGAGA/GGCCGC 1 -- ng510--------
rs8935731,2
H119323488(-) AATTCC/TTGCTC 1 -- ng315Minor allele frequency- T:0.00NA EU EA WA 498
rs7506711,2
C119323360(+) AAGAAT/CCCCCC 1 -- ng313Minor allele frequency- C:0.01NA 130
About this table

HapMap Linkage Disequilibrium images for EN1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 131290

2 Novoseek disease relationships for EN1 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
sudden infant death syndrome 40.71 1 9056537 (1)
congenital malformation 21.99 1 11815869 (1)
About this table

Genetic Association Database: EN1
Human Genome Epidemiology Navigator: EN1 (3 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/46 PubMed articles for EN1 gene (see all 46 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 2019 HGNC: 3342 AceView: EN1 Ensembl:ENSG00000163064 euGenes: HUgn2019
ECgene: EN1 H-InvDB: EN1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for EN1 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for EN1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for EN1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for EN1 Antibodies for EN1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for EN1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Recombinant Proteins
(EN1 / Engrailed 1)
 Antibodies (EN1 / Engrailed 1)
 Search OriGene for EN1
 Search Tocris compounds for EN1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for EN1

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