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EMP1 Gene

protein-coding   GIFtS: 56

GC12P013210
epithelial membrane protein 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
B4B 3
CL-20 1, 2, 3
EMP-1 2, 3
TMP 1, 2, 3, 5
Descriptions
Protein B4B 3
Tumor-associated membrane protein 3
epithelial membrane protein 1 2
External Ids
HGNC: 33331
Entrez Gene: 20122
UniProtKB: P548493
Ensembl: ENSG000001345317
Search outside databases for aliases for EMP1 gene

Previous GC identifers: GC12P013091 GC12P013494 GC12P013249 GC12P013240

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Gene Wiki entry for EMP1
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the EMP1 gene  

Entrez Gene cytogenetic band: 12p12.3   Ensembl cytogenetic band:  12p13.1   HGNC cytogenetic band: 12p12.3

EMP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P013210:     (about GC identifiers)

Start:
13,187,140 bp from pter
End:
13,260,975 bp from pter
Size:
73,836 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_009714.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: EMP1_HUMAN, P54849 (See protein sequence)
Recommended Name: Epithelial membrane protein 1  
Size: 157 amino acids; 17563 Da
Subcellular location: Membrane; Multi-pass membrane protein
Secondary accessions: O00681 Q13481 Q13834

REFSEQ proteins: NP_001414.1  

ENSEMBL proteins: 
ENSP00000379595 ENSP00000256951 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Human Recombinant Proteins from Abnova (EMP1)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005624 membrane fraction TAS8996089
GO:0016020 membrane IEA--
GO:0016021 integral to membrane TAS8884260
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Antibodies for EMP1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Monoclonal and Polyclonal Antibodies from Abnova (EMP1)
Novus Biologicals Antibody for EMP1

Assays for EMP1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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3 InterPro domains/families:
 IPR004032 PMP22_EMP_MP20
 IPR004031 PMP22/EMP/MP20/Claudin
 IPR003932 EMP_1


   GeneDecks  EMP1 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry P54849

ProtoNet protein and cluster: P54849

2 Blocks protein families:
IPB003932 Epithelial membrane protein EMP-1 signature
IPB004032 PMP-22/EMP/MP20 family


UniProtKB/Swiss-Prot: EMP1_HUMAN, P54849
Similarity: Belongs to the PMP-22/EMP/MP20 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EMP1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EMP1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001423

              Applied Biosystems Silencer® siRNAs for EMP1

              Sigma-Aldrich siRNA and siRNA Panels for EMP1  
                     Sigma-Aldrich shRNA for EMP1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for EMP1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001423
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001423
                                 untagged cDNA clone in CMV expression vector: NM_001423 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001423

Genatlas biochemistry entry for EMP1:
epithelial membrane protein 1,rabbit CL-20 homolog,tumor associated membrane protein,expressed in
mammary tumor and T cell lymphoma,c-Myc target
(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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5/6 Interacting proteins for EMP1 (ENSP000002569513) via UniProtKB, MINT, and/or STRING (see all 6 )
InteractantInteraction Details
GeneCardExternal ID(s)
PHEXENSP000003686823STRING (score=.878)
PTHENSP000002820913STRING (score=.843)
FGF23ENSP000002378373STRING (score=.759)
RDXENSP000003428303STRING (score=.748)
BGLAPENSP000003572593STRING (score=.71)
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4 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007275 multicellular organismal development TAS8996089
GO:0008283 cell proliferation TAS8996089
GO:0008544 epidermis development TAS7499420
GO:0016049 cell growth IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for EMP1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EMP1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EMP1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001423

              Sigma-Aldrich siRNA and siRNA Panels for EMP1  
                     Sigma-Aldrich shRNA for EMP1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001423  

REFSEQ mRNAs for EMP1 gene: 

NM_001423.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001423  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001423
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001423
                                 untagged cDNA clone in CMV expression vector: NM_001423 

Additional cDNA sequence: 

AK299388.1 AK308505.1 AK310798.1 AK312246.1 BC017854.1 BC047300.1 CR608144.1 U43916.1 
U77085.1 Y07909.1 Z50751.1 

23 DOTS entries:

DT.118516  DT.100882920  DT.209718  DT.121183529  DT.453065  DT.121183498  DT.100705808  DT.100882919 
DT.100882907  DT.95356869  DT.121183540  DT.97769860  DT.40268524  DT.121183424  DT.121183435  DT.121183439 
DT.121183491  DT.121183515  DT.95292525  DT.95356894  DT.121183520  DT.70102025  DT.91764028 

24/519 AceView cDNA sequences (see all 519 ):

CA429050 CA421863 U43916 AA384452 F13094 CN481103 BM974726 AA370297 
BQ897579 CB054682 AI379651 BU681388 CR608144 AA314702 CD364356 CA446854 
BU150998 CA441779 CA406080 BE742343 AL602524 AI435003 AI129735 BQ575560 

highest scoring ESTs for EMP1:

U43916 Y07909 AA046025 AA157008 AA303202 AA314702 AA343071 AA375282 AA386096 AA442410 

Unigene Cluster for EMP1:

Epithelial membrane protein 1
Hs.707901  [show with all ESTs]
Unigene Representative Sequence: NM_001423


GeneLoc Exon Structure

5/8 Alternative Splicing Database (ASD) splice patterns (SP) for EMP1 (see all 8 )

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b · 5c · 5d ^ 6a · 6b · 6c · 6d
SP1:                          -     -                 -     -     -                           
SP2:              -     -     -     -                 -     -     -                           
SP3:                          -     -                                                         
SP4:                                                  -     -     -                           
SP5:              -     -     -     -     -           -     -     -                           

About this scheme

ECgene alternative splicing isoforms for EMP1

2 Ensembl transcripts including schematic representations:
ENST00000396301  ENST00000256951  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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EMP1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for EMP1

1 / 2 / 3

7 probe-sets matching EMP1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
37762_at2, 3 U95-A 1 1.00 1.00 0.98 1.07 Y07909 1.00 1.00 1.00 1
1321_s_at2, 3 U95-A 1 1.00 1.00 0.96 0.94 U43916 1.00 1.00 1.00 1

201324_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001423 0.60 1.00 0.82 1

201325_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001423 0.60 1.00 0.82 1

1564796_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

201324_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

201325_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  EMP1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TAATTTGCAT

SOURCE GeneReport for Unigene cluster: Hs.707901

Expression variation in blood from EXPOLDB for EMP1

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for EMP1 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
EMP11   -- epithelial membrane protein 1 85.35(n)
86.62(a)
486676  XM_543803.2  XP_543803.2 
chimpanzee
(Pan troglodytes)
EMP11   -- epithelial membrane protein 1 99.15(n)
97.45(a)
741239  XM_001156226.1  XP_001156226.1 
cow
(Bos taurus)
LOC7864901   -- similar to epithelial membrane protein 1 82.17(n)
80.89(a)
786490  XM_001251759.1  XP_001251760.1 
rat
(Rattus norvegicus)
Emp11   -- epithelial membrane protein 1 77.28(n)
75.8(a)
25314  NM_012843.2  NP_036975.1 
mouse
(Mus musculus)
Emp11, 5 6 (65.00 cM)5
epithelial membrane protein 11, 5 79.83(n)1
77.07(a)1
137301  NM_010128.41  NP_034258.11 
 AF0555775  AK1354535  (see all 23)
About this table        Species with no ortholog for EMP1

ENSEMBL Gene Tree for EMP1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for EMP1 gene
EMP22  PMP222  EMP32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/103 NCBI SNPs in EMP1 are shown (see all 103 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 98)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 12 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs88851,2
A,C,F,H,O13260463(+) CTCATC/TCATTT 1 -- ut3116Minor allele frequency- N:0.00MN EA EU WA NA 3082
--
rs31910641,2
A,C,F,H13260841(+) TTTGCA/GTTACT 1 -- ut31 ese38Minor allele frequency- G:0.17MN EU EA WA NA 736
rs125813021,2
C,F,H13239513(+) taattT/Gtatca 1 -- ng51 tfbs38Minor allele frequency- G:0.17EU EA WA 840
rs73157251,2
C,F,H13239012(+) GGAGCG/ACCAGC 1 -- ng518Minor allele frequency- A:0.21EU EA WA 816
rs20690861,2
C,F,H13259589(+) CCTCAT/GGGAGA 1 -- ut31 ese34Minor allele frequency- G:0.17EU EA WA 418
rs344122221,2
C,F13257771(+) TGCCAG/ATGAAG 1 N/S mis15Minor allele frequency- A:0.02NA EU EA WA 498
rs79660781,2
F,H13260237(+) GTAGAC/TGAGCT 1 -- ut31 ese34Minor allele frequency- T:0.01EU EA WA 418
rs11312731,2
H13240973(+) GAGGCA/GGGTCC 1 -- ut51 ese33Minor allele frequency- G:0.00EA WA 298
rs37418141,2
H13260214(-) TACCAC/TAAGAG 1 -- ut315Minor allele frequency- T:0.01EA EU WA 1892
rs22910611,2
H13255744(-) ATGTGG/AACCAC 1 V/V syn1 ese35Minor allele frequency- A:0.01EA EU WA 1910
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HapMap Linkage Disequilibrium images for EMP1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 602333

4 Novoseek disease relationships for EMP1 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
breast cancer 22.39 1 17690741 (1)
glioma 15.24 1 16204036 (1)
tumors 2.31 1 17690741 (1)
cancer 0.00 1 17690741 (1)
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(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/42 PubMed articles for EMP1 gene (see all 42 ):
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 2012 HGNC: 3333 AceView: EMP1 Ensembl:ENSG00000134531 euGenes: HUgn2012
ECgene: EMP1 H-InvDB: EMP1
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for EMP1 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for EMP1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for EMP1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for EMP1 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for EMP1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for EMP1

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