EIF3E Gene
protein-coding GIFtS: 53
GC08M109284
|
|
eukaryotic translation initiation factor 3, subunit E
(Previous names: eukaryotic translation initiation factor 3, subunit 6 48kDa )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: INT6, EIF3S6)
|
 |
 | Services
| |
 |
Aliases &
Descriptions
for EIF3E
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases |
|---|
| EIF3-P48 2 | | EIF3S6 2, 3, 5 | | INT6 2, 3, 5 | | eIF3-p46 2 | | eIF3-p48 1 | | eIF3e 1, 3 |
| | | Descriptions |
|---|
| Eukaryotic translation initiation factor 3 subunit 6 3 | | Viral integration site protein INT-6 homolog 3 | | eIF-3 p48 3 | eukaryotic translation initiation factor 3, subunit 6
(48kD) 2 | | eukaryotic translation initiation factor 3, subunit 6 48kDa 1, 2 | | eukaryotic translation initiation factor 3, subunit E 2 | | mammary tumor-associated protein INT6 2 | | murine mammary tumor integration site 6 (oncogene homolog) 2 |
|
| |
Search outside databases for aliases for EIF3E gene |
Summaries
for EIF3E(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: EIF3E_HUMAN, P60228Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is
required for several steps in the initiation of protein synthesis. The eIF-3 complex associates
with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi
and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA
recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also
required for disassembly and recycling of posttermination ribosomal complexes and subsequently
prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for
nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from
translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the
proteasome-mediated degradation of these proteinsGene Wiki entry for EIF3E (EIF3S6) |
Genomic Location
for EIF3E
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences)
About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 8q22-q23 Ensembl cytogenetic band: 8q23.1 HGNC cytogenetic band: 8q22-q23EIF3E Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome 8 GeneLoc Exon Structure GeneLoc location for GC08M109284:
(about GC identifiers)
Start:
|
109,283,148 bp from pter |
End:
|
109,330,135 bp from pter |
Size:
|
46,988 bases |
Orientation:
|
minus strand |
RefSeq DNA sequence:- NC_000008.9 NT_008046.15
| Proteins
for EIF3E
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: EIF3E_HUMAN, P60228 (See
protein sequence)Recommended Name: Eukaryotic translation initiation factor 3 subunit E Size: 445 amino acids; 52221 Da
Subunit: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is
composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J,
EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is
composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and
module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of
module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that
binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of
nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of
FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.
Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27
and UPF2. Interacts with the HTLV-1 protein Tax-1
Subcellular location: Cytoplasm. Nucleus. Note=Localizes to PML bodies
Mass spectrometry: Mass=52131.8; Method=Unknown; Range=1-445; Source=PubMed:17322308;
Mass spectrometry: Mass=52133.4; Mass_error=0.2; Method=MALDI; Range=1-445; Source=PubMed:18599441;
Secondary accessions: O43902 Q64058 Q64059 Q64252 Q8WVK4Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity)1
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_001559.1
ENSEMBL proteins: ENSP00000220849
Human Recombinant Proteins  Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
3 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for EIF3E:
Assays for EIF3E: | Protein
Domains/
Families
for EIF3E(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry P60228
ProtoNet protein and cluster: P60228 UniProtKB/Swiss-Prot: EIF3E_HUMAN, P60228Similarity: Belongs to the eIF-3 subunit E familySimilarity: Contains 1 PCI domain | Gene Function
for EIF3E
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001568
 Applied Biosystems Silencer® siRNAs for EIF3E
 Sigma-Aldrich siRNA and siRNA Panels for EIF3E
Sigma-Aldrich shRNA for EIF3E
Explore Sigma-Aldrich super-pooled esiRNAs
OriGene GFP tagged cDNA clone in CMV expression vector: NM_001568
Myc/DDK tagged cDNA clone in CMV expression vector: NM_001568
untagged cDNA clones in CMV expression vector (see all 2): NM_001568
Primers:  Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
OriGene genome-wide validated SYBR primer pairs: NM_001568
UniProtKB/Swiss-Prot: EIF3E_HUMAN, P60228Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is
required for several steps in the initiation of protein synthesis. The eIF-3 complex associates
with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi
and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA
recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also
required for disassembly and recycling of posttermination ribosomal complexes and subsequently
prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for
nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from
translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the
proteasome-mediated degradation of these proteins2 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions
for EIF3E
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
 Gene Network CentralTM Interacting Genes and Proteins Network for EIF3E
5/133 Interacting proteins for EIF3E (P602281, 2 ENSP000002208493) via UniProtKB, MINT, and/or STRING (see all 133
)| Interactant | Interaction Details |
|---|
| GeneCard | External ID(s) |
|---|
| EIF3A | Q141521, ENSP000003581403 | EBI-347740, EBI-366617 STRING (score=.999) | | EIF3C | Q996131, ENSP000003326043 | EBI-347740, EBI-353741 STRING (score=.999) | | ENSG00000206318 | P486341, 2 | EBI-347740, EBI-347545 MINT-62621 MINT-62719 MINT-62795 | | ENSG00000206427 | P486341, 2 | EBI-347740, EBI-347545 MINT-62621 MINT-62719 MINT-62795 | | BAT2 | P486341, 2 | EBI-347740, EBI-347545 MINT-62621 MINT-62719 MINT-62795 EBI-347740, EBI-347545 MINT-62621 MINT-62719 MINT-62795 |
About this table
1 Gene Ontology (GO) biological process term (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006412 | translation |
IEA | -- |
About this table
|
Drugs & Compounds
for EIF3E(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
 Browse Tocris compounds for EIF3E
|
Transcripts
for EIF3E(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene,
Expression Assays from Applied Biosystems)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001568
Sigma-Aldrich siRNA and siRNA Panels for EIF3E
Sigma-Aldrich shRNA for EIF3E
Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer® siRNAs: NM_001568 REFSEQ mRNAs for EIF3E gene: NM_001568.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_001568
OriGene GFP tagged cDNA clone in CMV expression vector: NM_001568
Myc/DDK tagged cDNA clone in CMV expression vector: NM_001568
untagged cDNA clones in CMV expression vector (see all 2): NM_001568
Additional cDNA sequence: AK124178.1 AK310902.1 AK313186.1 BC000734.2 BC005944.1 BC008419.1 BC016706.1 BC017887.1 BC021679.1 CR457029.1 CR542275.1 CR595188.1 CR597834.1 CR598628.1 CR607803.1 CR608129.1 CR611386.1 CR611387.1 CR612349.1 CR613323.1 CR615385.1 CR617854.1 CR621066.1 CR621914.1 CR623723.1 CR625706.1 U54562.1 U62962.1 U85947.1 U94175.1 24/42 DOTS entries (see all 42
): DT.91873781 DT.91786530 DT.95109944 DT.40195311 DT.100040612 DT.91898358 DT.100887390 DT.100887394 DT.120632323 DT.97860893 DT.120632268 DT.99992062 DT.100887404 DT.121383379 DT.91970237 DT.100887382 DT.92331786 DT.100816985 DT.100887385 DT.120632257 DT.92470692 DT.100799277 DT.100887388 DT.120632266 24/1388 AceView cDNA sequences (see all 1388
):AU107669 AW080381 CB107613 CB142571 CB159693 AI567374 BM710722 CB120386 BG740315 AL533501 AA576696 CA430848 CN481735 BM454003 BQ421431 BU521027 BQ574105 BG821284 AA132321 BM450298 AA129221 BU155088 BU164182 AU117306 Unigene Cluster for EIF3E: Eukaryotic translation initiation factor 3, subunit E Hs.405590 [show with all ESTs]Unigene Representative Sequence: AK124178
GeneLoc Exon Structure
3 Alternative Splicing Database (ASD) splice patterns (SP) for EIF3E
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | ^ | 5 | ^ | 6 |
|---|
|
| SP1: | | | | | - | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for EIF3E
1 Ensembl transcript including schematic representation: ENST00000220849
|
Expression
for EIF3E
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| EIF3E expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for EIF3E
1 / 2 4 probe-sets matching EIF3E gene Data from
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: AATATTGAGA
SOURCE GeneReport for Unigene cluster: Hs.405590
UniProtKB/Swiss-Prot: EIF3E_HUMAN, P60228Tissue specificity: Ubiquitously expressed. Expressed at highest levels in appendix, lymph,
pancreas, skeletal muscle, spleen and thymus |
Orthologs
for EIF3E
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for EIF3E gene from 5/19 species (see all 19
)
About this table Species with no ortholog for EIF3E
ENSEMBL Gene Tree for EIF3E | Paralogs
for EIF3E(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| -- |
SNPs/Variants
for EIF3E(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for EIF3E (up to first 250kb)
|
Disorders & Mutations
for EIF3E
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 602210
4  Novoseek disease relationships for EIF3E gene
About this table
|
Medical News
for EIF3E(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for EIF3E (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/53 PubMed articles for EIF3E gene (see all 53
):- The translation initiation factor eIF3-p48 subunit is encoded by int-6, a site of frequent integration by the mouse mammary tumor virus genome. (PubMed id 9295280)1, 2, 3, 4 Asano K....Hershey J.W. (1997)
- The chromosome location of the human homolog of the mouse mammary tumor-associated gene INT6 and its status in human breast carcinomas. (PubMed id 9403073)2, 3, 4 Miyazaki S.... Callahan R. (1997)
- Interaction between the Ret finger protein and the Int-6 gene product and co-localisation into nuclear bodies. (PubMed id 10504338)1, 3, 4 Morris-Desbois C.... Jalinot P. (1999)
- The human protein HSPC021 interacts with Int-6 and is associated with eukaryotic translation initiation factor 3. (PubMed id 11590142)1, 3, 4 Morris-Desbois C....Jalinot P. (2001)
- Human INT6/eIF3e is required for nonsense-mediated mRNA decay. (PubMed id 17468741)1, 3, 4 Morris C....Jalinot P. (2007)
- Association of the mammalian proto-oncoprotein Int-6 with the three protein complexes eIF3, COP9 signalosome and 26S proteasome. (PubMed id 12220626)1, 3, 4 Hoareau Alves K.... Jalinot P. (2002)
- Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit. (PubMed id 16766523)1, 3, 4 LeFebvre A.K....Rhoads R.E. (2006)
- Human INT6 interacts with MCM7 and regulates its stability during S phase of the cell cycle. (PubMed id 17310990)3, 4 Buchsbaum S....Jalinot P. (2007)
- Mammalian tumor suppressor Int6 specifically targets hypoxia inducible factor 2 alpha for degradation by hypoxia- and pVHL-independent regulation. (PubMed id 17324924)3, 4 Chen L....Shibasaki F. (2007)
- Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. (PubMed id 15592455)3, 4 Rush J.... Comb M.J. (2005)
|
Search for EIF3E
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing EIF3E
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing EIF3E
(According to HUGE)
About This Section
| -- |
Specialized Databases showing EIF3E(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| About This Section
| --
| Services
for EIF3E(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section
| 
Products for EIF3E:
 | |
 | | | | |
 |
 |  |  | |
| | |  | Search Tocris compounds for EIF3E |
|
 |
 | | |
|
GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009
|
V3 here soon-try beta now
