EIF3B Gene
protein-coding GIFtS: 57
GCID: GC07P002362
|
|
eukaryotic translation initiation factor 3, subunit B(Previous names: eukaryotic translation initiation factor 3, subunit 9 eta,...) (Previous symbol: EIF3S9)
| |
Aliases for EIF3B gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Eukaryotic Translation Initiation Factor 3, Subunit B1 2 | | EIF3 P1102 3 | | EIF3S91 2 3 5 | | EIF3 P1162 3 | | PRT11 2 5 | | HPrt11 | | Eukaryotic Translation Initiation Factor 3, Subunit 9 Eta, 116kDa1 2 | | EIF3-ETA2 | | EIF3b1 | | EIF3-P1102 | | Eukaryotic Translation Initiation Factor 3 Subunit 92 3 | | EIF3-P1162 | | Prt1 Homolog2 3 | | Eukaryotic Translation Initiation Factor 3 Subunit B2 | | EIF-3-Eta1 | | Eukaryotic Translation Initiation Factor 3, Subunit 9 (Eta, 116kD)2 |
Export aliases for EIF3B gene to outside databases |
Summaries for EIF3B gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: EIF3B_HUMAN, P55884Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for severalsteps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates therecruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). TheeIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequentlyprevents premature joining of the 40S and 60S ribosomal subunits prior to initiation Gene Wiki entry for EIF3B
|
Genomic Views for EIF3B gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000007.13 NC_018918.1 NT_007819.17 NT_079592.2
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the EIF3B gene promoter: Max1 Pax-5 NF-E2 p45 Egr-4 AREB6 Pax-4a NF-E2 ATF ATF6 c-Myc Other transcription factors
Search SABiosciences Chromatin IP Primers for EIF3B
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat EIF3B |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 7p22.3 Ensembl cytogenetic band: 7p22.3 HGNC cytogenetic band: 7p22EIF3B Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 7 GeneLoc Exon Structure GeneLoc location for GC07P002362: view genomic region
(about GC identifiers)
Start:
|
2,393,721 bp from pter |
End:
|
2,420,380 bp from pter |
Size:
|
26,660 bases |
Orientation:
|
plus strand |
1 alternative location:
| Chr7+,CRA_TCAG 2,442,541-2,468,274 |
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Proteins for EIF3B gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: EIF3B_HUMAN, P55884 (See
protein sequence)Recommended Name: Eukaryotic translation initiation factor 3 subunit B Size: 814 amino acids; 92482 Da
Subunit: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits:EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complexappears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed ofEIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C bindsEIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds tothe eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion.Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading tophosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2
Subcellular location: Cytoplasm (By similarity)
Mass spectrometry: Mass=93093.7; Method=Unknown; Range=1-814; Source=PubMed:17322308;
Mass spectrometry: Mass=92561; Mass_error=43.5; Method=MALDI; Range=1-814; Source=PubMed:18599441;
2 PDB 3D structures from and Proteopedia for EIF3B:2KRB (3D)
  2NLW (3D)
 
Secondary accessions: A4D208 Q2NL77 Q9UMF9Alternative splicing: 2 isoforms: P55884-1 P55884-2 Explore the universe of human proteins at neXtProt for EIF3B: NX_P55884
Post-translational modifications:
Phosphorylated. Phosphorylation is enhanced upon serum stimulation1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P55884 EIF3B Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001032360.1 NP_003742.2 ENSEMBL proteins: ENSP00000408062 ENSP00000354125 ENSP00000407785 ENSP00000380206 Reactome Protein details: P55884 Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
EIF3B for ontologies About GeneDecksing
EIF3B Antibody Products: Assay Products for EIF3B: |
Protein
Domains / Families for EIF3B gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
EIF3B for domains About GeneDecksing
5 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry P55884ProtoNet protein and cluster: P55884 2 Blocks protein families: IPB000504 RNA-binding region RNP-1 (RNA recognition motif) IPB013979 Eukaryotic translation initiation factor 2A
UniProtKB/Swiss-Prot: EIF3B_HUMAN, P55884Domain: The RRM domain mediates interaction with EIF3JSimilarity: Belongs to the eIF-3 subunit B familySimilarity: Contains 1 RRM (RNA recognition motif) domainSimilarity: Contains 5 WD repeats |
Function for EIF3B gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: EIF3B_HUMAN, P55884Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for severalsteps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates therecruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). TheeIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequentlyprevents premature joining of the 40S and 60S ribosomal subunits prior to initiation
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for EIF3B (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for EIF3B (see all 3) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): EIF3B (NM_003751) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for EIF3B | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat EIF3B  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EIF3B |
Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table
EIF3B for ontologies About GeneDecksing
5 GenomeRNAi human phenotypes for EIF3B:
|
Pathways & Interactions for EIF3B gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
| Unified GeneCards pathways - 5/7 super-pathways (see all 7) About this table  See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Synthesis of PB1-F2 | | | 2 | Translation initiation complex formation | | | 3 | IL-2 Signaling Pathway | | | 4 | Translation Factors | | | 5 | RNA transport | |
Pathway sources See GeneCards unified pathways Show all pathways
2 BioSystems Pathways for EIF3B 
5/13
Reactome Pathways for EIF3B (see all 13)
1
Kegg Pathway (Kegg details for EIF3B):
EIF3B for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for EIF3B
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
 5/735 Interacting proteins for EIF3B (P558841, 2, 3 ENSP000003541254) via UniProtKB, MINT, STRING, and/or I2D (see all 735)About this table
Gene Ontology (GO): 5 biological process terms (GO ID links to tree view): About this table
EIF3B for ontologies About GeneDecksing
|
Drugs & Compounds for EIF3B gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
Browse Tocris compounds for EIF3B Search CenterWatch for drugs/clinical trials and news about EIF3B 
|
Transcripts for EIF3B gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for EIF3B gene (2 alternative transcripts): NM_001037283.1 NM_003751.3 Unigene Cluster for EIF3B: Eukaryotic translation initiation factor 3, subunit B Hs.371001 [show with all ESTs]Unigene Representative Sequence: AB20938112 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000431643 ENST00000360876(uc003slx.3 uc003sly.3 uc003slz.1) ENST00000413917 ENST00000397011(uc003sma.3) ENST00000463229 ENST00000463026 ENST00000468250 ENST00000466199 ENST00000475415 ENST00000468611(uc003smb.3) ENST00000465670 ENST00000494658
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for EIF3B (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for EIF3B (see all 3) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): EIF3B (NM_003751) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for EIF3B | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat EIF3B  |
Additional cDNA sequence: AB209381.1 AK127885.1 AK300967.1 AK302058.1 BC001173.1 BC009986.1 BC110865.1 U62583.1 U78525.1 24/35 DOTS entries (see all 35): DT.100834695 DT.97798019 DT.100759692 DT.95173385 DT.100834690 DT.454061 DT.100730058 DT.429116 DT.92462641 DT.97786597 DT.203336 DT.70104982 DT.102836765 DT.100039603 DT.95305235 DT.95148198 DT.97864515 DT.121124337 DT.95313948 DT.100834693 DT.121124353 DT.100033517 DT.100834684 DT.100834692 24/758 AceView cDNA sequences (see all 758): AW613698 BQ221471 BM560380 BF108826 BQ709516 AW015861 AI825123 CB115033 AI204368 BC001173 BQ669015 CA434118 BP368238 BU175745 CD673946 BU856686 AI699769 CR603939 AI336324 BQ643260 AU124102 AW473838 BM904776 BU956074 GeneLoc Exon Structure
5/18 Alternative Splicing Database (ASD) splice patterns (SP) for EIF3B (see all 18) About this scheme
| ExUns: | 1 | ^ | 2 | ^ | 3a | · | 3b | · | 3c | ^ | 4a | · | 4b | ^ | 5a | · | 5b | ^ | 6a | · | 6b | ^ | 7a | · | 7b | ^ | 8a | · | 8b | ^ | 9a | · | 9b | · | 9c | ^ | 10 | ^ | 11a | · | 11b | ^ | 12a | · | 12b | ^ | 13 | ^ | 14 | ^ | 15a | · | |
| SP1: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | - |   | |   | - |   | - |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | - |   | |   | - |   | - |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | - |   | |   | - |   | - |   | |   | |   | |
| SP4: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | - |   | |   | - |   | - |   | |   | |   | |
| SP5: | |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
| ExUns: | 15b | · | 15c | ^ | 16 | ^ | 17a | · | 17b | ^ | 18 | ^ | 19a | · | 19b | · | 19c | ^ | 20a | · | 20b | ^ | 21 | ^ | 22a | · | 22b | ^ | 23a | · | 23b | ^ | 24a | · | 24b | · | 24c | |
| SP1: | |   | - |   | |   | - |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | - |   | - |   | |   | - |   | |   | |   | |
| SP2: | |   | - |   | |   | - |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |
| SP3: | |   | - |   | |   | - |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |
| SP4: | |   | - |   | |   | - |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |
| SP5: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
ECgene alternative splicing isoforms for EIF3B
|
Expression for EIF3B gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| EIF3B expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: GAAATAAAAG
About this image See EIF3B Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for EIF3B
SOURCE GeneReport for Unigene cluster: Hs.371001 SABiosciences Custom PCR Arrays for EIF3B
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for EIF3B Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat EIF3B | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat EIF3B | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat EIF3B | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EIF3B |
Orthologs for EIF3B gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for EIF3B gene from 9/36 species (see all 36) About this table
| Organism |
Taxonomic classification |
Gene |
Description |
Human Similarity |
Orthology Type |
Details |
chicken (Gallus gallus) |
Aves |
EIF3B1 |
eukaryotic translation initiation factor 3, subunit more |
79.62(n) 91.02(a) |
  |
769783 XM_003642142.1 XP_003642190.1 |
lizard (Anolis carolinensis) |
Reptilia |
-- |
-- |
81(a) |
1 ↔ 1 |
GL343340.1(42357-51899) |
African clawed frog (Xenopus laevis) |
Amphibia |
Xl.260732 |
Xenopus laevis transcribed sequence with moderate similarity more |
77.37(n) |
  |
48055327 |
zebrafish (Danio rerio) |
Actinopterygii |
wufc17c012 |
wufc17c01 |
82.82(n) |
  |
324028 CA787533.1 |
fruit fly (Drosophila melanogaster) |
Insecta |
eIF3-S91 |
CG4878-PA |
58.47(n) 53.76(a) |
  |
36981 NM_137384.3 NP_611228.1 |
worm (Caenorhabditis elegans) |
Secernentea |
eif-3.B1 |
Protein EIF-3.B |
46.13(n) 36.6(a) |
  |
175138 NM_001027298.3 NP_001022469.1 |
baker's yeast (Saccharomyces cerevisiae) |
Saccharomycetes |
PRT1(YOR361C)4 PRT11 |
eIF3b subunit of the core complex of translation initiation more4 Prt1p1 |
47.92(n)1 34.28(a)1 |
  |
15(1017652-1015361)4 8545431, 4 NP_015006.31 NP_015006.14 |
thale cress (Arabidopsis thaliana) |
eudicotyledons |
TIF3B11 |
translation initiation factor 3 subunit B |
53.14(n) 43.23(a) |
  |
832826 NM_001036877.2 NP_001031954.1 |
rice (Oryza sativa) |
Liliopsida |
Os.48992 |
Oryza sativa (japonica cultivar-group) cDNA clone0 more |
70.36(n) |
  |
AK110143.1 |
ENSEMBL Gene Tree for EIF3B (if available) TreeFam Gene Tree for EIF3B (if available)  |
Paralogs for EIF3B gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| -- |
Genomic Variants for EIF3B gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 7 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
|---|
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