EIF3B Gene

Aliases
for EIF3B gene

Summaries
for EIF3B gene

Genomic Views
for EIF3B gene

RefSeq DNA sequence:

NC_000007.13  NC_018918.1  NT_007819.17  NT_079592.2  

SwitchGear Promoter luciferase reporter plasmids (see all 11): EIF3B promoter sequence

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat EIF3B

EIF3B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)


GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07P002362:  view genomic region     (about GC identifiers)

Proteins
for EIF3B gene

2 PDB 3D structures from and Proteopedia for EIF3B:

2KRB (3D)        2NLW (3D)    

Explore the universe of human proteins at neXtProt for EIF3B: NX_P55884

Post-translational modifications:

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	Uscn Proteins for EIF3B

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

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	OriGene Antibodies (see all 2): EIF3B OriGene Custom Antibody Services for EIF3B
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	Abcam antibodies for EIF3B
	Uscn Antibodies for EIF3B
	ThermoFisher Antibody for EIF3B

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	Uscn ELISAs and CLIAs for EIF3B

Protein Domains /
Families
for EIF3B gene

ProtoNet protein and cluster: P55884

Function
for EIF3B gene

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		6 QIAGEN miScript miRNA Assays for microRNAs that regulate EIF3B:
		hsa-miR-3140-3p hsa-miR-124 hsa-miR-198 hsa-miR-525-5p hsa-miR-506 hsa-miR-520a-5p
		SwitchGear 3'UTR luciferase reporter plasmid: EIF3B 3' UTR sequence

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Gene Editing Products:

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Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for EIF3B (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for EIF3B (see all 3) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
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In Situ Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EIF3B

Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

Pathways & Interactions
for EIF3B gene

	Super-pathway	contained gene-specific pathways
1	Synthesis of PB1-F2	Formation of a pool of free 40S subunits 0.87 Eukaryotic Translation Initiation 0.73 L13a-mediated translational silencing of Ceruloplasmin expression 0.79 Cap-dependent Translation Initiation 0.73 3' -UTR-mediated translational regulation 0.79 Translation 0.57 GTP hydrolysis and joining of the 60S ribosomal subunit 0.78
2	Translation initiation complex formation	Translation initiation complex formation 1.00 Ribosomal scanning and start codon recognition 0.96 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S 0.98 Formation of the ternary complex, and subsequently, the 43S complex 0.87
3	IL-2 Signaling Pathway	IL-2 Signaling Pathway 1.00
4	Translation Factors	Translation Factors 1.00
5	RNA transport	RNA transport 1.00

2 BioSystems Pathways for EIF3B

	Translation Factors
	IL-2 Signaling Pathway

5/13        Reactome Pathways for EIF3B

	Formation of the ternary complex, and subsequently, the 43S complex
	Translation
	GTP hydrolysis and joining of the 60S ribosomal subunit
	Eukaryotic Translation Initiation
	Ribosomal scanning and start codon recognition

1         Kegg Pathway  (Kegg details for EIF3B)

RNA transport

Gene Ontology (GO): 5 biological process terms (GO ID links to tree view):    About this table

Drugs & Compounds
for EIF3B gene

Transcripts
for EIF3B gene

Expression
for EIF3B gene

EIF3B expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GAAATAAAAG

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for EIF3B Browse OriGene validated miRNA SYBR primer pairs
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In Situ Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EIF3B

Orthologs
for EIF3B gene

Paralogs
for EIF3B gene

Genomic Variants
for EIF3B gene

10/761 NCBI SNPs in EIF3B are shown (see all 761)     About this table

Genomic Data					Transcription Related Data				Allele Frequencies
SNP ID	Valid	Clinical significance	Chr 7 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
-1st2nd3rd	-1st2nd3rd	-1st2nd3rd	-1st2nd3rd	--	--	--	-1st2nd3rd	--	--	-1st2nd3rd	-1st2nd3rd	-1st2nd3rd
rs77959371,2	C,	--	2392502(+)	cttatC/Tgtaca	2	--	us2k1		0	--	--	--	--
rs1866445271,2		--	2392544(+)	ACCTGC/TCCAAA	2	--	us2k1		0	--	--	--	--
rs1911089171,2		--	2392571(+)	CATGAC/TGAGGA	2	--	us2k1		0	--	--	--	--
rs1405732621,2		--	2392678(+)	AGTCCC/TGGCAC	2	--	us2k1		0	--	--	--	--
rs749343721,2	F,	--	2392748(+)	ACACAG/ATGAGA	2	--	us2k1		1		NA	120
rs1457357701,2		--	2392785(+)	TGGGCA/GCGGTG	2	--	us2k1		0	--	--	--	--
rs624441621,2	C,	--	2392860(+)	GCATAT/CTAAAA	2	--	us2k1		2		NA	122
rs1825669971,2		--	2392876(+)	AGGCCC/GTGCGC	2	--	us2k1		0	--	--	--	--
rs1418556821,2		--	2392881(+)	CTGCGC/GGGTGG	2	--	us2k1		0	--	--	--	--
rs77929341,2	C,F,	--	2393016(+)	ACCCTG/ATCTCT	2	--	us2k1		12		NA WA CSA EA	374

HapMap Linkage Disequilibrium report for EIF3B (2393721 - 2420380 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 9 variations for EIF3B
     2 CNVs: 69768 99846
     7 Indels: 94341 12902 81845 69769 12901 28608 12900

SABiosciences Cancer Mutation PCR Assays

Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing EIF3B

DNA2.0 Custom Variant and Variant Library Synthesis for EIF3B

Disorders / Diseases
for EIF3B gene

Publications
for EIF3B gene

PubMed articles for EIF3B gene, integrated from 9 sources (see all 73) (see top 10): (articles sorted by number of sources associating them with EIF3B)

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1. The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170. (PubMed id 8995410)^{1, 2, 3, 9} Methot N.... Sonenberg N. (1997)
2. Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7. (PubMed id 11169732)^{1, 2, 9} Lin L.... Burger M.M. (2001)
3. Biochemical characterization of mammalian translation initiation factor 3 (eIF3). Molecular cloning reveals that p110 subunit is the mammalian homologue of Saccharomyces cerevisiae protein Prt1. (PubMed id 9388245)^{1, 2, 9} Chaudhuri J.... Maitra U. (1997)
4. Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. (PubMed id 18599441)^{1, 2} Zhou M....Robinson C.V. (2008)
5. Reconstitution reveals the functional core of mammalian eIF3. (PubMed id 17581632)^{1, 2} Masutani M.... Imataka H. (2007)
6. Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry. (PubMed id 17322308)^{1, 2} Damoc E....Leary J.A. (2007)
7. Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit. (PubMed id 17190833)^{1, 2} ElAntak L.... Lukavsky P.J. (2007)
8. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)^{1, 2} Olsen J.V....Mann M. (2006)
9. mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events. (PubMed id 16286006)^{1, 2} Holz M.K.... Blenis J. (2005)
10. The j-subunit of human translation initiation factor eIF3 is required for the stable binding of eIF3 and its subcomplexes to 40 S ribosomal subunits in vitro. (PubMed id 14688252)^{1, 2} Fraser C.S....Hershey J.W. (2004)
11. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
12. Human chromosome 7: DNA sequence and biology. (PubMed id 12690205)^{1, 2} Scherer S.W.... Tsui L.-C. (2003)
13. Characterization of eIF3k: a newly discovered subunit of mammalian translation initiation factor elF3. (PubMed id 14519125)^{1, 2} Mayeur G.L....Hershey J.W. (2003)
14. A census of human soluble protein complexes. (PubMed id 22939629)¹ Havugimana P.C....Emili A. (2012)
15. Charting the landscape of tandem BRCT domain-mediated protein interactions. (PubMed id 22990118)¹ Woods N.T....Monteiro A.N. (2012)
16. A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)¹ Kristensen A.R....Foster L.J. (2012)
17. Global landscape of HIV-human protein complexes. (PubMed id 22190034)¹ Jager S....Krogan N.J. (2012)
18. A cohesin-RAD21 interactome. (PubMed id 22145905)¹ Panigrahi A.K....Pati D. (2012)
19. Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)¹ Udeshi N.D....Carr S.A. (2012)
20. Initial characterization of the human central proteome. (PubMed id 21269460)² Burkard T.R.... Colinge J. (2011)
21. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)¹ Wagner S.A....Choudhary C. (2011)
22. A data set of human endogenous protein ubiquitination sites. (PubMed id 20972266)¹ Shi Y....Qin J. (2011)
23. Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyc losome substrate. (PubMed id 21628590)¹ Emanuele M.J....Elledge S.J. (2011)
24. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)¹ Kim W....Gygi S.P. (2011)
25. Nuclear import of histone deacetylase 5 by requisite nuclear localization signal phosphorylation. (PubMed id 21081666)¹ Greco T.M....Cristea I.M. (2011)
26. Ubiquitin ligase substrate identification through qua ntitative proteomics at both the protein and peptide levels. (PubMed id 21987572)¹ Lee K.A....Doedens J.R. (2011)
27. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)¹ Danielsen J.M....Nielsen M.L. (2011)
28. System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)² Rigbolt K.T....Blagoev B. (2011)
29. Systematic analysis of human protein complexes identi fies chromosome segregation proteins. (PubMed id 20360068)¹ Hutchins J.R....Peters J.M. (2010)
30. ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes. (PubMed id 20508642)¹ Krebs A.R....Tora L. (2010)
31. Crystal structure of the RNA recognition motif of yea st translation initiation factor eIF3b reveals differences to human eIF3b. (PubMed id 20862284)¹ Khoshnevis S....Ficner R. (2010)
32. The indispensable N-terminal half of eIF3j/HCR1 coope rates with its structurally conserved binding partner eIF3b/PRT1-RRM and with e IF1A in stringent AUG selection. (PubMed id 20060839)¹ Elantak L....ValA!sek L. (2010)
33. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)² Olsen J.V....Mann M. (2010)
34. mTORC2 can associate with ribosomes to promote cotran slational phosphorylation and stability of nascent Akt polypeptide. (PubMed id 21045808)¹ Oh W.J....Jacinto E. (2010)
35. Phosphorylation of the eukaryotic initiation factor 3f by cyclin-dependent kinase 11 during apoptosis. (PubMed id 19245811)¹ Shi J....Nelson M.A. (2009)
36. Defining the human deubiquitinating enzyme interactio n landscape. (PubMed id 19615732)¹ Sowa M.E....Harper J.W. (2009)
37. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (PubMed id 19690332)² Mayya V.... Han D.K. (2009)
38. In vitro nuclear interactome of the HIV-1 Tat protein . (PubMed id 19454010)¹ Gautier V.W....Hall W.W. (2009)
39. Lysine acetylation targets protein complexes and co-regulates major cellular functions. (PubMed id 19608861)² Choudhary C.... Mann M. (2009)
40. A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)² Dephoure N.... Gygi S.P. (2008)
41. Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry. (PubMed id 18781797)¹ Meierhofer D.... Kaiser P. (2008)
42. Identification of intracellular proteins associated w ith the EBV-encoded nuclear antigen 5 using an efficient TAP procedure and FT-I CR mass spectrometry. (PubMed id 18457437)¹ Forsman A....Rymo L. (2008)
43. Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. (PubMed id 18220336)² Cantin G.T....Yates J.R. III (2008)
44. Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. (PubMed id 18628297)¹ Lee C.S....Reed R. (2008)
45. CIF-1, a shared subunit of the COP9/signalosome and eukaryotic initiation factor 3 complexes, regulates MEL-26 levels in the Caenorhabditis elegans embryo. (PubMed id 17403899)¹ Luke-Glaser S.... Pintard L. (2007)
46. Human INT6/eIF3e is required for nonsense-mediated mRNA decay. (PubMed id 17468741)² Morris C....Jalinot P. (2007)
47. mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin. (PubMed id 16541103)¹ Harris T.E....Lawrence J.C. (2006)
48. S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth. (PubMed id 17053147)¹ Dorrello N.V....Pagano M. (2006)
49. Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit. (PubMed id 16766523)² LeFebvre A.K.... Rhoads R.E. (2006)
50. Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association. (PubMed id 15703437)² Kolupaeva V.G....Pestova T.V. (2005)
51. Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. (PubMed id 15592455)¹ Rush J.... Comb M.J. (2005)
52. Large-scale analysis of the human ubiquitin-related p roteome. (PubMed id 16196087)¹ Matsumoto M....Nakayama K.I. (2005)
53. Structural roles for human translation factor eIF3 in initiation of protein synthesis. (PubMed id 16322461)² Siridechadilok B....Nogales E. (2005)
54. Proteomic analysis of SRm160-containing complexes reveals a conserved association with cohesin. (PubMed id 16159877)¹ McCracken S....Blencowe B.J. (2005)
55. Changes in ribosomal binding activity of eIF3 correlate with increased translation rates during activation of T lymphocytes. (PubMed id 15946946)¹ Miyamoto S....Hershey J.W. (2005)
56. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)¹ Ota T.... Sugano S. (2004)
57. Large-scale characterization of HeLa cell nuclear phosphoproteins. (PubMed id 15302935)¹ Beausoleil S.A....Gygi S.P. (2004)
58. Analysis of proteins copurifying with the CD4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: comparison with affinity-tag based protein detection and evaluation of different solubilization methods. (PubMed id 15047060)¹ Bernhard O.K....Sheil M.M. (2004)
59. Transcript-selective translational silencing by gamma interferon is directed by a novel structural element in the ceruloplasmin mRNA 3' untranslated region. (PubMed id 12588972)¹ Sampath P....Fox P.L. (2003)
60. The DNA sequence of human chromosome 7. (PubMed id 12853948)² Hillier L.W.... Wilson R.K. (2003)
61. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)¹ Strausberg R.L....Marra M.A. (2002)
62. Multiple roles for the C-terminal domain of eIF5 in translation initiation complex assembly and GTPase activation. (PubMed id 11331597)¹ Asano K....Hinnebusch A.G. (2001)
63. Saccharomyces cerevisiae protein Pci8p and human protein eIF3e/Int-6 interact with the eIF3 core complex by binding to cognate eIF3b subunits. (PubMed id 11457827)² Shalev A.... Asano K. (2001)
64. Characterization of cDNAs encoding the p44 and p35 subunits of human translation initiation factor eIF3. (PubMed id 9822659)¹ Block K.L.... Hershey J.W.B. (1998)

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