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Aliases for EHMT2 Gene

Aliases for EHMT2 Gene

  • Euchromatic Histone-Lysine N-Methyltransferase 2 2 3 4
  • NG36 3 4 6
  • G9A 3 4 6
  • Histone H3-K9 Methyltransferase 3 3 4
  • HLA-B Associated Transcript 8 2 3
  • Lysine N-Methyltransferase 1C 3 4
  • H3-K9-HMTase 3 3 4
  • C6orf30 3 4
  • KMT1C 3 4
  • BAT8 3 4
  • GAT8 3 6
  • Histone-Lysine N-Methyltransferase, H3 Lysine-9 Specific 3 3
  • Histone-Lysine N-Methyltransferase EHMT2 3
  • Chromosome 6 Open Reading Frame 30 2
  • Ankyrin Repeat-Containing Protein 3
  • G9A Histone Methyltransferase 3
  • HLA-B-Associated Transcript 8 4
  • Protein G9a 4
  • EC 2.1.1.43 4
  • EC 2.1.1.- 4

External Ids for EHMT2 Gene

Previous Symbols for EHMT2 Gene

  • C6orf30
  • BAT8

Summaries for EHMT2 Gene

Entrez Gene Summary for EHMT2 Gene

  • This gene is located in a gene cluster within the human major histocompatibility complex class III region on chromosome 6. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but recent publications show that there is only a single gene at this locus. The encoded protein is a histone methyltransferase, which may be involved in repression of transcription. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

GeneCards Summary for EHMT2 Gene

EHMT2 (Euchromatic Histone-Lysine N-Methyltransferase 2) is a Protein Coding gene. Among its related pathways are Cellular Senescence and Gene Expression. GO annotations related to this gene include p53 binding and protein-lysine N-methyltransferase activity. An important paralog of this gene is EHMT1.

UniProtKB/Swiss-Prot for EHMT2 Gene

  • Histone methyltransferase that specifically mono- and dimethylates Lys-9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of Lys-56 of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates Lys-27 of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of Lys-373 of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Tocris Summary for EHMT2 Gene

  • Histone methyltransferases (HMTs) are a group of enyzmes that catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to histones and are closely related in biological activity to the DNA methyltransferases. Histone methylation occurs predominantly on the side chains of lysine and arginine residues in histones H3 and H4, and multiple methyl groups may be added to each residue. Histone methyltransferases can be subdivided according to their target residue: those which methylate lysine residues on histone proteins are known as protein lysine methyltransferases (PKMTs) whereas those acting on arginine residues are known as protein arginine methyltransferases (PRMTs).

Gene Wiki entry for EHMT2 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for EHMT2 Gene

Genomics for EHMT2 Gene

Genomic Location for EHMT2 Gene

Start:
31,879,759 bp from pter
End:
31,897,707 bp from pter
Size:
17,949 bases
Orientation:
Minus strand

Genomic View for EHMT2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for EHMT2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

Regulatory Elements for EHMT2 Gene

Proteins for EHMT2 Gene

  • Protein details for EHMT2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96KQ7-EHMT2_HUMAN
    Recommended name:
    Histone-lysine N-methyltransferase EHMT2
    Protein Accession:
    Q96KQ7
    Secondary Accessions:
    • B0UZY2
    • Q14349
    • Q5JP83
    • Q5JQ92
    • Q5JQA1
    • Q5JQG3
    • Q6PK06
    • Q96MH5
    • Q96QD0
    • Q9UQL8
    • Q9Y331

    Protein attributes for EHMT2 Gene

    Size:
    1210 amino acids
    Molecular mass:
    132370 Da
    Quaternary structure:
    • Heterodimer; heterodimerizes with EHMT1/GLP. Interacts with GFI1B and WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with UHRF1. Interacts with CDYL. Interacts with REST only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2.
    SequenceCaution:
    • Sequence=AAD21811.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=AAD21812.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=AAH02686.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAH09351.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAH18718.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH20970.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=BAB63294.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=BAB63295.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAA49491.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for EHMT2 Gene

    Alternative splice isoforms for EHMT2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for EHMT2 Gene

Proteomics data for EHMT2 Gene at MOPED

Post-translational modifications for EHMT2 Gene

Other Protein References for EHMT2 Gene

No data available for DME Specific Peptides for EHMT2 Gene

Domains for EHMT2 Gene

Gene Families for EHMT2 Gene

HGNC:
  • ANKRD :Ankyrin repeat domain containing
  • KMT :Chromatin-modifying enzymes / K-methyltransferases

UniProtKB/Swiss-Prot:

EHMT2_HUMAN
Domain:
  • The SET domain mediates interaction with WIZ.:
    • Q96KQ7
  • The ANK repeats bind H3K9me1 and H3K9me2.:
    • Q96KQ7
  • In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.:
    • Q96KQ7
  • Contains 1 post-SET domain.:
    • Q96KQ7
  • Contains 1 pre-SET domain.:
    • Q96KQ7
  • Contains 1 SET domain.:
    • Q96KQ7
Family:
  • Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.:
    • Q96KQ7
Similarity:
  • Contains 7 ANK repeats.:
    • Q96KQ7
genes like me logo Genes that share domains with EHMT2: view

Function for EHMT2 Gene

Molecular function for EHMT2 Gene

UniProtKB/Swiss-Prot CatalyticActivity: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
UniProtKB/Swiss-Prot Function: Histone methyltransferase that specifically mono- and dimethylates Lys-9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of Lys-56 of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates Lys-27 of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of Lys-373 of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Enzyme Numbers (IUBMB) for EHMT2 Gene

Gene Ontology (GO) - Molecular Function for EHMT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002039 p53 binding IPI 20118233
GO:0005515 protein binding IPI 16189514
GO:0008270 zinc ion binding IEA --
GO:0016279 protein-lysine N-methyltransferase activity IDA 20118233
GO:0018024 histone-lysine N-methyltransferase activity IMP 22387026
genes like me logo Genes that share ontologies with EHMT2: view
genes like me logo Genes that share phenotypes with EHMT2: view

Animal Models for EHMT2 Gene

MGI Knock Outs for EHMT2:

miRNA for EHMT2 Gene

No data available for Transcription Factor Targeting and HOMER Transcription for EHMT2 Gene

Localization for EHMT2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for EHMT2 Gene

Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for EHMT2 Gene COMPARTMENTS Subcellular localization image for EHMT2 gene
Compartment Confidence
nucleus 5
cytoskeleton 3
cytosol 2
mitochondrion 1

Gene Ontology (GO) - Cellular Components for EHMT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin IDA 19144645
GO:0005634 nucleus ISS --
GO:0005654 nucleoplasm TAS --
GO:0005694 chromosome --
GO:0043231 intracellular membrane-bounded organelle IDA --
genes like me logo Genes that share ontologies with EHMT2: view

Pathways for EHMT2 Gene

genes like me logo Genes that share pathways with EHMT2: view

Pathways by source for EHMT2 Gene

1 R&D Systems pathway for EHMT2 Gene
1 Cell Signaling Technology pathway for EHMT2 Gene
1 BioSystems pathway for EHMT2 Gene

Gene Ontology (GO) - Biological Process for EHMT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IDA 19144645
GO:0006275 regulation of DNA replication IMP 22387026
GO:0006306 DNA methylation ISS --
GO:0006325 chromatin organization TAS --
GO:0006357 regulation of transcription from RNA polymerase II promoter --
genes like me logo Genes that share ontologies with EHMT2: view

Compounds for EHMT2 Gene

(5) Tocris Compounds for EHMT2 Gene

Compound Action Cas Number
3-Deazaneplanocin A hydrochloride Histone methyltransferase inhibitor [120964-45-6]
BIX 01294 G9a-like protein and G9a histone lysine methyltransferase inhibitor [935693-62-2]
SGC 0946 Highly potent and selective DOT1L inhibitor; cell permeable
UNC 0638 Selective G9a and GLP histone lysine methyltransferase inhibitor [1255517-77-1]
UNC 0646 Potent and selective G9a/GLP inhibitor [1320288-17-2]

(3) HMDB Compounds for EHMT2 Gene

Compound Synonyms Cas Number PubMed IDs
L-Lysine
  • (+)-S-Lysine
56-87-1
S-Adenosylhomocysteine
  • (S)-5'-(S)-(3-Amino-3-carboxypropyl)-5'-thioadenosine
979-92-0
S-Adenosylmethionine
  • (3S)-5'-[(3-amino-3-carboxypropyl)methylsulfonio]-5'-deoxyadenosine
29908-03-0

(1) Novoseek inferred chemical compound relationships for EHMT2 Gene

Compound -log(P) Hits PubMed IDs
lysine 59.1 7
genes like me logo Genes that share compounds with EHMT2: view

Transcripts for EHMT2 Gene

Unigene Clusters for EHMT2 Gene

Euchromatic histone-lysine N-methyltransferase 2:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for EHMT2 Gene

No ASD Table

Relevant External Links for EHMT2 Gene

GeneLoc Exon Structure for
EHMT2
ECgene alternative splicing isoforms for
EHMT2

Expression for EHMT2 Gene

mRNA expression in normal human tissues for EHMT2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for EHMT2 Gene

SOURCE GeneReport for Unigene cluster for EHMT2 Gene Hs.709218

mRNA Expression by UniProt/SwissProt for EHMT2 Gene

Q96KQ7-EHMT2_HUMAN
Tissue specificity: Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
genes like me logo Genes that share expressions with EHMT2: view

Orthologs for EHMT2 Gene

This gene was present in the common ancestor of animals.

Orthologs for EHMT2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia EHMT2 36
  • 99.36 (n)
  • 99.47 (a)
EHMT2 37
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia BAT8 37
  • 92 (a)
OneToOne
EHMT2 36
  • 91.98 (n)
  • 97.9 (a)
dog
(Canis familiaris)
Mammalia EHMT2 36
  • 90.85 (n)
  • 96.99 (a)
EHMT2 37
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ehmt2 36
  • 88.03 (n)
  • 95.61 (a)
Ehmt2 16
Ehmt2 37
  • 90 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia EHMT2 37
  • 92 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia EHMT2 37
  • 68 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ehmt2 36
  • 87.78 (n)
  • 96.03 (a)
lizard
(Anolis carolinensis)
Reptilia EHMT2 37
  • 70 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.7568 36
tropical clawed frog
(Silurana tropicalis)
Amphibia ehmt2 36
  • 66.19 (n)
  • 71.08 (a)
Str.3432 36
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.7796 36
zebrafish
(Danio rerio)
Actinopterygii bat8 36
ehmt2 36
  • 63.46 (n)
  • 63.98 (a)
ehmt2 37
  • 53 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta G9a 37
  • 20 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.2694 36
sea squirt
(Ciona savignyi)
Ascidiacea CSA.117 37
  • 43 (a)
OneToMany
Species with no ortholog for EHMT2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for EHMT2 Gene

ENSEMBL:
Gene Tree for EHMT2 (if available)
TreeFam:
Gene Tree for EHMT2 (if available)

Paralogs for EHMT2 Gene

Selected SIMAP similar genes for EHMT2 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with EHMT2: view

Variants for EHMT2 Gene

Sequence variations from dbSNP and Humsavar for EHMT2 Gene

SNP ID Clin Chr 06 pos Sequence Context AA Info Type MAF
rs486416 -- 31,888,293(-) CATGT(C/T)GTTTC intron-variant
rs535586 -- 31,892,560(-) TAGTC(A/G)GAGGA reference, synonymous-codon
rs555007 -- 31,882,555(-) CCCCT(A/G)TTCCT intron-variant
rs608118 -- 31,886,587(-) GTCAA(C/T)GCCCA reference, synonymous-codon
rs610265 -- 31,886,096(-) ttttt(C/T)ttttt intron-variant

Structural Variations from Database of Genomic Variants (DGV) for EHMT2 Gene

Variant ID Type Subtype PubMed ID
dgv6583n71 CNV Loss 21882294
nsv884472 CNV Loss 21882294
nsv508399 CNV Loss 20534489
nsv509126 CNV Insertion 20534489
dgv6595n71 CNV Loss 21882294
nsv884476 CNV Gain 21882294
dgv6596n71 CNV Loss 21882294
dgv6597n71 CNV Loss 21882294
dgv6598n71 CNV Loss 21882294
nsv884486 CNV Loss 21882294
dgv6599n71 CNV Loss 21882294
dgv6600n71 CNV Loss 21882294
dgv6601n71 CNV Loss 21882294

Relevant External Links for EHMT2 Gene

HapMap Linkage Disequilibrium report
EHMT2
Human Gene Mutation Database (HGMD)
EHMT2

Disorders for EHMT2 Gene

(2) Novoseek inferred disease relationships for EHMT2 Gene

Disease -log(P) Hits PubMed IDs
cancer 0.271 3
tumors 0 3

Relevant External Links for EHMT2

Genetic Association Database (GAD)
EHMT2
Human Genome Epidemiology (HuGE) Navigator
EHMT2
genes like me logo Genes that share disorders with EHMT2: view

No data available for UniProtKB/Swiss-Prot for EHMT2 Gene

Publications for EHMT2 Gene

  1. The G9a gene in the human major histocompatibility complex encodes a novel protein containing ankyrin-like repeats. (PMID: 8457211) Milner C.M. … Campbell R.D. (Biochem. J. 1993) 2 3 4 23
  2. Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3. (PMID: 11316813) Tachibana M. … Shinkai Y. (J. Biol. Chem. 2001) 2 3 4 23
  3. Novel NG36/G9a gene products encoded within the human and mouse MHC class III regions. (PMID: 11707778) Brown S.E. … Sanderson C.M. (Mamm. Genome 2001) 3 4 23
  4. UHRF1 binds G9a and participates in p21 transcriptional regulation in mammalian cells. (PMID: 19056828) Kim J.K. … Pradhan S. (Nucleic Acids Res. 2009) 3 4 23
  5. G9a and Glp methylate lysine 373 in the tumor suppressor p53. (PMID: 20118233) Huang J. … Berger S.L. (J. Biol. Chem. 2010) 3 4 23

Products for EHMT2 Gene

  • Addgene plasmids for EHMT2

Sources for EHMT2 Gene

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