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Aliases for EEF2K Gene

Aliases for EEF2K Gene

  • Eukaryotic Elongation Factor 2 Kinase 2 3 5
  • Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase 3 4
  • EEF-2 Kinase 3 4
  • EC 2.7.11.20 4 63
  • EEF-2K 3 4
  • Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor-2 Kinase 3
  • Calmodulin-Dependent Protein Kinase III 3
  • Eukaroytic Elongation Factor 2 Kinase 3
  • Eukaryotic Elongation Factor-2 Kinase 3
  • Elongation Factor-2 Kinase 3
  • EC 2.7.11 63
  • HSU93850 3

External Ids for EEF2K Gene

Previous GeneCards Identifiers for EEF2K Gene

  • GC16P021638
  • GC16P022227
  • GC16P022184
  • GC00U916980
  • GC16P022125
  • GC16P020462

Summaries for EEF2K Gene

Entrez Gene Summary for EEF2K Gene

  • This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

GeneCards Summary for EEF2K Gene

EEF2K (Eukaryotic Elongation Factor 2 Kinase) is a Protein Coding gene. Diseases associated with EEF2K include anoxia. Among its related pathways are Signaling by GPCR and AMPK signaling pathway. GO annotations related to this gene include calcium ion binding and protein kinase activity. An important paralog of this gene is ALPK1.

UniProtKB/Swiss-Prot for EEF2K Gene

  • Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Tocris Summary for EEF2K Gene

  • Calmodulin-dependent kinases (CaMK) are a family of serine/threonine kinases that mediate many of the second messenger effects of Ca2+. At basal Ca2+ levels, CaMKs are maintained in a dormant state through autoinhibition, which can be relieved by increases in Ca2+ levels.

Gene Wiki entry for EEF2K Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for EEF2K Gene

Genomics for EEF2K Gene

Regulatory Elements for EEF2K Gene

Promoters for EEF2K Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around EEF2K on UCSC Golden Path with GeneCards custom track

Genomic Location for EEF2K Gene

Chromosome:
16
Start:
22,206,271 bp from pter
End:
22,288,745 bp from pter
Size:
82,475 bases
Orientation:
Plus strand

Genomic View for EEF2K Gene

Genes around EEF2K on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
EEF2K Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for EEF2K Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for EEF2K Gene

Proteins for EEF2K Gene

  • Protein details for EEF2K Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O00418-EF2K_HUMAN
    Recommended name:
    Eukaryotic elongation factor 2 kinase
    Protein Accession:
    O00418
    Secondary Accessions:
    • Q8N588

    Protein attributes for EEF2K Gene

    Size:
    725 amino acids
    Molecular mass:
    82144 Da
    Quaternary structure:
    • Monomer or homodimer.

neXtProt entry for EEF2K Gene

Proteomics data for EEF2K Gene at MOPED

Selected DME Specific Peptides for EEF2K Gene

Post-translational modifications for EEF2K Gene

  • Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.
  • Ubiquitination at Lys 65, Lys 162, Lys 238, Lys 341, Lys 485, Lys 603, and Lys 705
  • Modification sites at PhosphoSitePlus

Other Protein References for EEF2K Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for EEF2K Gene

Gene Families for EEF2K Gene

Suggested Antigen Peptide Sequences for EEF2K Gene

Graphical View of Domain Structure for InterPro Entry

O00418

UniProtKB/Swiss-Prot:

EF2K_HUMAN :
  • The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain.
  • Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Domain:
  • The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain.
  • Contains 1 alpha-type protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
genes like me logo Genes that share domains with EEF2K: view

Function for EEF2K Gene

Molecular function for EEF2K Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.
UniProtKB/Swiss-Prot Function:
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Enzyme Numbers (IUBMB) for EEF2K Gene

Gene Ontology (GO) - Molecular Function for EEF2K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004674 protein serine/threonine kinase activity IEA --
GO:0005516 calmodulin binding IEA,IDA 9144159
genes like me logo Genes that share ontologies with EEF2K: view
genes like me logo Genes that share phenotypes with EEF2K: view

Animal Models for EEF2K Gene

MGI Knock Outs for EEF2K:

Animal Model Products

miRNA for EEF2K Gene

Inhibitory RNA Products

In Situ Assay Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for EEF2K Gene

Localization for EEF2K Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for EEF2K Gene COMPARTMENTS Subcellular localization image for EEF2K gene
Compartment Confidence
cytosol 5
nucleus 2
extracellular 1
golgi apparatus 1

No data available for Subcellular locations from UniProtKB/Swiss-Prot and Gene Ontology (GO) - Cellular Components for EEF2K Gene

Pathways & Interactions for EEF2K Gene

genes like me logo Genes that share pathways with EEF2K: view

SIGNOR curated interactions for EEF2K Gene

Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for EEF2K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006414 translational elongation TAS 9144159
GO:0006468 protein phosphorylation IEA --
GO:0008286 insulin receptor signaling pathway TAS --
GO:0043066 negative regulation of apoptotic process IEA --
GO:0045807 positive regulation of endocytosis IEA --
genes like me logo Genes that share ontologies with EEF2K: view

Drugs & Compounds for EEF2K Gene

(14) Drugs for EEF2K Gene - From: Novoseek, ApexBio, Tocris, and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
calcium Nutra 0
NH125 Pharma Selective eEF-2 kinase inhibitor, CaM kinase III (eEF-2 kinase) inhibitor 0
KN-62 Pharma CaM kinase II inhibitor, Non-competitive P2X7 antagonist 0

(6) Additional Compounds for EEF2K Gene - From: Tocris and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Arcyriaflavin A
118458-54-1
Autocamtide-2-related inhibitory peptide
167114-91-2
KN 93
139298-40-1
STO-609 acetate
1173022-21-3

(5) Tocris Compounds for EEF2K Gene

Compound Action Cas Number
Arcyriaflavin A Potent cdk4/cyclin D1 and CaM Kinase II inhibitor. Antiviral agent (anti-HCMV) 118458-54-1
Autocamtide-2-related inhibitory peptide Selective CaM kinase II inhibitor 167114-91-2
KN 93 CaM kinase II inhibitor; also K+ channel blocker (KV) 139298-40-1
KN-62 Non-competitive P2X7 antagonist 127191-97-3
STO-609 acetate Selective CaM kinase kinase inhibitor 1173022-21-3

(1) ApexBio Compounds for EEF2K Gene

Compound Action Cas Number
NH125 Selective eEF-2 kinase inhibitor 278603-08-0
genes like me logo Genes that share compounds with EEF2K: view

Transcripts for EEF2K Gene

Unigene Clusters for EEF2K Gene

Eukaryotic elongation factor-2 kinase:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for EEF2K Gene

No ASD Table

Relevant External Links for EEF2K Gene

GeneLoc Exon Structure for
EEF2K
ECgene alternative splicing isoforms for
EEF2K

Expression for EEF2K Gene

mRNA expression in normal human tissues for EEF2K Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for EEF2K Gene

This gene is overexpressed in Skin - Sun Exposed (Lower leg) (x4.1).

Protein differential expression in normal tissues from HIPED for EEF2K Gene

This gene is overexpressed in Liver, secretome (19.2), Lung (8.9), and Prostate (6.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for EEF2K Gene



SOURCE GeneReport for Unigene cluster for EEF2K Gene Hs.498892

genes like me logo Genes that share expression patterns with EEF2K: view

Primer Products

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt and Protein tissue co-expression partners for EEF2K Gene

Orthologs for EEF2K Gene

This gene was present in the common ancestor of animals.

Orthologs for EEF2K Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia EEF2K 35
  • 88.8 (n)
  • 90.87 (a)
EEF2K 36
  • 91 (a)
OneToOne
dog
(Canis familiaris)
Mammalia EEF2K 35
  • 89.56 (n)
  • 93.24 (a)
EEF2K 36
  • 93 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Eef2k 35
  • 85.91 (n)
  • 90.19 (a)
Eef2k 16
Eef2k 36
  • 90 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia EEF2K 35
  • 99.36 (n)
  • 99.03 (a)
EEF2K 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Eef2k 35
  • 85.73 (n)
  • 90.33 (a)
oppossum
(Monodelphis domestica)
Mammalia EEF2K 36
  • 84 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia EEF2K 36
  • 85 (a)
OneToOne
chicken
(Gallus gallus)
Aves EEF2K 35
  • 74.25 (n)
  • 78.9 (a)
EEF2K 36
  • 79 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia EEF2K 36
  • 71 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia eef2k 35
  • 69.92 (n)
  • 74.06 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.18633 35
zebrafish
(Danio rerio)
Actinopterygii eef2k 35
  • 66.31 (n)
  • 69.51 (a)
eef2k 36
  • 62 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea efk-1 37
  • 63 (a)
efk-1 35
  • 49.28 (n)
  • 45.79 (a)
efk-1 36
  • 36 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.10624 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 44 (a)
ManyToMany
CSA.7709 36
  • 43 (a)
ManyToMany
Species with no ortholog for EEF2K:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for EEF2K Gene

ENSEMBL:
Gene Tree for EEF2K (if available)
TreeFam:
Gene Tree for EEF2K (if available)

Paralogs for EEF2K Gene

Paralogs for EEF2K Gene

genes like me logo Genes that share paralogs with EEF2K: view

Variants for EEF2K Gene

Sequence variations from dbSNP and Humsavar for EEF2K Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type
rs9935059 - 22,225,797(+) TGGCC(A/G)TGATG nc-transcript-variant, reference, missense
rs17841292 - 22,225,952(+) GGTCC(C/G)CGGCA nc-transcript-variant, reference, missense
rs147978363 A colorectal adenocarcinoma sample 22,257,356(+) GGAGA(C/T)GGGCA nc-transcript-variant, reference, missense
rs56137739 - 22,260,527(+) ACCAC(A/C/T)GGGTG nc-transcript-variant, reference, synonymous-codon, missense
VAR_041536 -

Structural Variations from Database of Genomic Variants (DGV) for EEF2K Gene

Variant ID Type Subtype PubMed ID
nsv7280 OTHER Inversion 18451855
dgv3n1 OTHER Inversion 15895083
nsv511047 OTHER Inversion 20534489
dgv47n16 OTHER Inversion 17901297
nsv499139 OTHER Inversion 21111241
esv998989 OTHER Inversion 20482838
esv2714070 CNV Deletion 23290073
dgv48n16 CNV Insertion 17901297
nsv1764 CNV Loss 18451855
nsv435668 CNV Deletion 17901297
nsv498860 CNV Loss 21111241
dgv2647n71 CNV Loss 21882294
nsv905598 CNV Gain 21882294
nsv519813 CNV Gain+Loss 19592680
nsv469818 CNV Complex 16826518
esv2662229 CNV Deletion 23128226
dgv834e1 CNV Complex 17122850

Variation tolerance for EEF2K Gene

Residual Variation Intolerance Score: 46.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.91; 79.61% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for EEF2K Gene

HapMap Linkage Disequilibrium report
EEF2K
Human Gene Mutation Database (HGMD)
EEF2K

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for EEF2K Gene

Disorders for EEF2K Gene

MalaCards: The human disease database

(1) MalaCards diseases for EEF2K Gene - From: GeneCards

Disorder Aliases PubMed IDs
anoxia
- elite association - COSMIC cancer census association via MalaCards
Search EEF2K in MalaCards View complete list of genes associated with diseases

Relevant External Links for EEF2K

Genetic Association Database (GAD)
EEF2K
Human Genome Epidemiology (HuGE) Navigator
EEF2K
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
EEF2K
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
EEF2K
genes like me logo Genes that share disorders with EEF2K: view

No data available for UniProtKB/Swiss-Prot and Genatlas for EEF2K Gene

Publications for EEF2K Gene

  1. Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. (PMID: 9144159) Ryazanov A.G. … Hait W.N. (Proc. Natl. Acad. Sci. U.S.A. 1997) 2 3 4 23 67
  2. Cytoprotective effect of the elongation factor-2 kinase-mediated autophagy in breast cancer cells subjected to growth factor inhibition. (PMID: 20300520) Cheng Y. … Yang J. (PLoS ONE 2010) 3 23
  3. Immune reactions against elongation factor 2 kinase: specific pathogenesis of gastric ulcer from Helicobacter pylori infection. (PMID: 19636416) Ayada K. … Oguma K. (Clin. Dev. Immunol. 2009) 3 23
  4. Levels of mTOR and its downstream targets 4E-BP1, eEF2, and eEF2 kinase in relationships with tau in Alzheimer's disease brain. (PMID: 16098202) Li X. … Pei J.J. (FEBS J. 2005) 3 23
  5. Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398. (PMID: 14709557) Browne G.J. … Proud C.G. (J. Biol. Chem. 2004) 3 23

Products for EEF2K Gene

Sources for EEF2K Gene

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