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Aliases for EEF2K Gene

Aliases for EEF2K Gene

  • Eukaryotic Elongation Factor 2 Kinase 2 3
  • Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase 3 4
  • EEF-2 Kinase 3 4
  • EC 2.7.11.20 4 63
  • EEF-2K 3 4
  • Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor-2 Kinase 3
  • Calmodulin-Dependent Protein Kinase III 3
  • Eukaroytic Elongation Factor 2 Kinase 3
  • Eukaryotic Elongation Factor-2 Kinase 3
  • Elongation Factor-2 Kinase 3
  • EC 2.7.11 63
  • HSU93850 3

External Ids for EEF2K Gene

Previous GeneCards Identifiers for EEF2K Gene

  • GC16P021638
  • GC16P022227
  • GC16P022184
  • GC00U916980
  • GC16P022125
  • GC16P020462

Summaries for EEF2K Gene

Entrez Gene Summary for EEF2K Gene

  • This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

GeneCards Summary for EEF2K Gene

EEF2K (Eukaryotic Elongation Factor 2 Kinase) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and mTOR signalling. GO annotations related to this gene include calcium ion binding and protein kinase activity. An important paralog of this gene is ALPK1.

UniProtKB/Swiss-Prot for EEF2K Gene

  • Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Tocris Summary for EEF2K Gene

  • Calmodulin-dependent kinases (CaMK) are a family of serine/threonine kinases that mediate many of the second messenger effects of Ca2+. At basal Ca2+ levels, CaMKs are maintained in a dormant state through autoinhibition, which can be relieved by increases in Ca2+ levels.

Gene Wiki entry for EEF2K Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for EEF2K Gene

Genomics for EEF2K Gene

Regulatory Elements for EEF2K Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for EEF2K Gene

Chromosome:
16
Start:
22,206,271 bp from pter
End:
22,288,745 bp from pter
Size:
82,475 bases
Orientation:
Plus strand

Genomic View for EEF2K Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for EEF2K Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for EEF2K Gene

Proteins for EEF2K Gene

  • Protein details for EEF2K Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O00418-EF2K_HUMAN
    Recommended name:
    Eukaryotic elongation factor 2 kinase
    Protein Accession:
    O00418
    Secondary Accessions:
    • Q8N588

    Protein attributes for EEF2K Gene

    Size:
    725 amino acids
    Molecular mass:
    82144 Da
    Quaternary structure:
    • Monomer or homodimer.

neXtProt entry for EEF2K Gene

Proteomics data for EEF2K Gene at MOPED

Selected DME Specific Peptides for EEF2K Gene

Post-translational modifications for EEF2K Gene

  • Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.
  • Ubiquitination at Lys 65, Lys 162, Lys 238, Lys 341, Lys 485, Lys 603, and Lys 705
  • Modification sites at PhosphoSitePlus

Other Protein References for EEF2K Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for EEF2K Gene

Gene Families for EEF2K Gene

Suggested Antigen Peptide Sequences for EEF2K Gene

Graphical View of Domain Structure for InterPro Entry

O00418

UniProtKB/Swiss-Prot:

EF2K_HUMAN :
  • The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain.
  • Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Domain:
  • The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain.
  • Contains 1 alpha-type protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
genes like me logo Genes that share domains with EEF2K: view

Function for EEF2K Gene

Molecular function for EEF2K Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.
UniProtKB/Swiss-Prot Function:
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Enzyme Numbers (IUBMB) for EEF2K Gene

Gene Ontology (GO) - Molecular Function for EEF2K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity TAS 9144159
GO:0004674 protein serine/threonine kinase activity --
GO:0004686 elongation factor-2 kinase activity IDA 9144159
GO:0005509 calcium ion binding IEA --
GO:0005516 calmodulin binding IDA 9144159
genes like me logo Genes that share ontologies with EEF2K: view
genes like me logo Genes that share phenotypes with EEF2K: view

Animal Models for EEF2K Gene

MGI Knock Outs for EEF2K:

Animal Model Products

miRNA for EEF2K Gene

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for EEF2K

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targets and HOMER Transcription for EEF2K Gene

Localization for EEF2K Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for EEF2K Gene COMPARTMENTS Subcellular localization image for EEF2K gene
Compartment Confidence
cytosol 4

Gene Ontology (GO) - Cellular Components for EEF2K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm TAS 9144159
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with EEF2K: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for EEF2K Gene

Pathways & Interactions for EEF2K Gene

genes like me logo Genes that share pathways with EEF2K: view

SIGNOR curated interactions for EEF2K Gene

Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for EEF2K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006414 translational elongation TAS 9144159
GO:0006468 protein phosphorylation --
GO:0008286 insulin receptor signaling pathway TAS --
GO:0031952 regulation of protein autophosphorylation IEA --
GO:0046777 protein autophosphorylation IDA 9144159
genes like me logo Genes that share ontologies with EEF2K: view

Drugs & Compounds for EEF2K Gene

(13) Drugs for EEF2K Gene - From: NovoSeek, ApexBio, Tocris, and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
anisomycin Experimental Pharma JNK, SAPK and p38 activator 0
cyclic amp Experimental Pharma 0
Forskolin Experimental Pharma 0
NH125 Pharma Selective eEF-2 kinase inhibitor, CaM kinase III (eEF-2 kinase) inhibitor 0
Calcium Nutra 0

(6) Additional Compounds for EEF2K Gene - From: Tocris and NovoSeek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
A 484954
142557-61-7
KN 93
139298-40-1
KN 93 phosphate
1188890-41-6
STO-609 acetate
1173022-21-3
glucose
genes like me logo Genes that share compounds with EEF2K: view

Transcripts for EEF2K Gene

mRNA/cDNA for EEF2K Gene

Unigene Clusters for EEF2K Gene

Eukaryotic elongation factor-2 kinase:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for EEF2K

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for EEF2K Gene

No ASD Table

Relevant External Links for EEF2K Gene

GeneLoc Exon Structure for
EEF2K
ECgene alternative splicing isoforms for
EEF2K

Expression for EEF2K Gene

mRNA expression in normal human tissues for EEF2K Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for EEF2K Gene

This gene is overexpressed in Skin - Sun Exposed (Lower leg) (x4.1).

Protein differential expression in normal tissues from HIPED for EEF2K Gene

This gene is overexpressed in Liver, secretome (19.2), Lung (8.9), and Prostate (6.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for EEF2K Gene



SOURCE GeneReport for Unigene cluster for EEF2K Gene Hs.498892

genes like me logo Genes that share expression patterns with EEF2K: view

Primer Products

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt and Protein tissue co-expression partners for EEF2K Gene

Orthologs for EEF2K Gene

This gene was present in the common ancestor of animals.

Orthologs for EEF2K Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia EEF2K 35
  • 88.8 (n)
  • 90.87 (a)
EEF2K 36
  • 91 (a)
OneToOne
dog
(Canis familiaris)
Mammalia EEF2K 35
  • 89.56 (n)
  • 93.24 (a)
EEF2K 36
  • 93 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Eef2k 35
  • 85.91 (n)
  • 90.19 (a)
Eef2k 16
Eef2k 36
  • 90 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia EEF2K 35
  • 99.36 (n)
  • 99.03 (a)
EEF2K 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Eef2k 35
  • 85.73 (n)
  • 90.33 (a)
oppossum
(Monodelphis domestica)
Mammalia EEF2K 36
  • 84 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia EEF2K 36
  • 85 (a)
OneToOne
chicken
(Gallus gallus)
Aves EEF2K 35
  • 74.25 (n)
  • 78.9 (a)
EEF2K 36
  • 79 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia EEF2K 36
  • 71 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia eef2k 35
  • 69.92 (n)
  • 74.06 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.18633 35
zebrafish
(Danio rerio)
Actinopterygii eef2k 35
  • 66.31 (n)
  • 69.51 (a)
eef2k 36
  • 62 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea efk-1 37
  • 63 (a)
efk-1 35
  • 49.28 (n)
  • 45.79 (a)
efk-1 36
  • 36 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.10624 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 44 (a)
ManyToMany
CSA.7709 36
  • 43 (a)
ManyToMany
Species with no ortholog for EEF2K:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for EEF2K Gene

ENSEMBL:
Gene Tree for EEF2K (if available)
TreeFam:
Gene Tree for EEF2K (if available)

Paralogs for EEF2K Gene

Paralogs for EEF2K Gene

genes like me logo Genes that share paralogs with EEF2K: view

Variants for EEF2K Gene

Sequence variations from dbSNP and Humsavar for EEF2K Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type MAF
rs716304 -- 22,260,787(+) CAACA(A/G)TGCGA intron-variant
rs754290 -- 22,212,188(-) TCATC(A/G)TGCAA intron-variant
rs868431 -- 22,220,072(-) ACGTG(A/G)CCACT intron-variant
rs868800 -- 22,240,191(+) CAACA(A/G)GGAGC intron-variant
rs929849 -- 22,210,165(-) TGGGG(A/G)GCACT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for EEF2K Gene

Variant ID Type Subtype PubMed ID
nsv7280 OTHER Inversion 18451855
dgv3n1 OTHER Inversion 15895083
nsv511047 OTHER Inversion 20534489
dgv47n16 OTHER Inversion 17901297
nsv499139 OTHER Inversion 21111241
esv998989 OTHER Inversion 20482838
esv2714070 CNV Deletion 23290073
dgv48n16 CNV Insertion 17901297
nsv1764 CNV Loss 18451855
nsv435668 CNV Deletion 17901297
nsv498860 CNV Loss 21111241
dgv2647n71 CNV Loss 21882294
nsv905598 CNV Gain 21882294
nsv519813 CNV Gain+Loss 19592680
nsv469818 CNV Complex 16826518
esv2662229 CNV Deletion 23128226
dgv834e1 CNV Complex 17122850

Variation tolerance for EEF2K Gene

Residual Variation Intolerance Score: 46.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.91; 79.61% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for EEF2K Gene

HapMap Linkage Disequilibrium report
EEF2K
Human Gene Mutation Database (HGMD)
EEF2K

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for EEF2K Gene

Disorders for EEF2K Gene

Relevant External Links for EEF2K

Genetic Association Database (GAD)
EEF2K
Human Genome Epidemiology (HuGE) Navigator
EEF2K
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
EEF2K
genes like me logo Genes that share disorders with EEF2K: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for EEF2K Gene

Publications for EEF2K Gene

  1. Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. (PMID: 9144159) Ryazanov A.G. … Hait W.N. (Proc. Natl. Acad. Sci. U.S.A. 1997) 2 23 67
  2. Inhibition of eEF2-K by thieno[2,3-b]pyridine analogues. (PMID: 20189382) Lockman J.W. … Adam Willardsen J. (Bioorg. Med. Chem. Lett. 2010) 23 67
  3. Cytoprotective effect of the elongation factor-2 kinase-mediated autophagy in breast cancer cells subjected to growth factor inhibition. (PMID: 20300520) Cheng Y. … Yang J. (PLoS ONE 2010) 23 67
  4. Resveratrol ameliorates high glucose-induced protein synthesis in glomerular epithelial cells. (PMID: 19765649) Lee M.J. … Kasinath B.S. (Cell. Signal. 2010) 23 67
  5. Synaptic activity controls dendritic spine morphology by modulating eEF2-dependent BDNF synthesis. (PMID: 20427644) Verpelli C. … Sala C. (J. Neurosci. 2010) 23 67

Products for EEF2K Gene

  • Addgene plasmids for EEF2K

Sources for EEF2K Gene

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