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Aliases for EEF2K Gene

Aliases for EEF2K Gene

  • Eukaryotic Elongation Factor 2 Kinase 2 3
  • Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase 3 4
  • EEF-2 Kinase 3 4
  • EC 2.7.11.20 4 64
  • EEF-2K 3 4
  • Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor-2 Kinase 3
  • Calmodulin-Dependent Protein Kinase III 3
  • Eukaroytic Elongation Factor 2 Kinase 3
  • Eukaryotic Elongation Factor-2 Kinase 3
  • Elongation Factor-2 Kinase 3
  • EC 2.7.11 64
  • HSU93850 3

External Ids for EEF2K Gene

Summaries for EEF2K Gene

Entrez Gene Summary for EEF2K Gene

  • This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

GeneCards Summary for EEF2K Gene

EEF2K (Eukaryotic Elongation Factor 2 Kinase) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Insulin receptor signalling cascade. GO annotations related to this gene include calcium ion binding and calmodulin binding. An important paralog of this gene is ALPK1.

UniProtKB/Swiss-Prot for EEF2K Gene

  • Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Tocris Summary for EEF2K Gene

  • Calmodulin-dependent kinases (CaMK) are a family of serine/threonine kinases that mediate many of the second messenger effects of Ca2+. At basal Ca2+ levels, CaMKs are maintained in a dormant state through autoinhibition. Increases in Ca2+ levels allows calmodulin to relieve this autoinhibition and activate the kinase activity. There are two main classes of CaMKs; mulitfunctional CaMKs (CaMKK, CaMKI, CaMKII and CaMKIV) which have multiple downstream targets and substrate-specific CaMKs (CaMKIII) which have only one known downstream target. All CaMKs, with the exception of CaMKII, exist as monomers and most are expressed ubiquitously. Some subtypes display specific distributions, for example, CaMKIVbeta is expressed exclusively in cerebellar granule cells. CaMKs have numerous cellular functions and they influence processes as diverse as gene transcription, cell survival, apoptosis, cytoskeletal re-organization and learning and memory.

Gene Wiki entry for EEF2K Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for EEF2K Gene

Genomics for EEF2K Gene

Genomic Location for EEF2K Gene

Start:
22,206,271 bp from pter
End:
22,288,745 bp from pter
Size:
82,475 bases
Orientation:
Plus strand

Genomic View for EEF2K Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for EEF2K Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for EEF2K Gene

Regulatory Elements for EEF2K Gene

Proteins for EEF2K Gene

  • Protein details for EEF2K Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O00418-EF2K_HUMAN
    Recommended name:
    Eukaryotic elongation factor 2 kinase
    Protein Accession:
    O00418
    Secondary Accessions:
    • Q8N588

    Protein attributes for EEF2K Gene

    Size:
    725 amino acids
    Molecular mass:
    82144 Da
    Quaternary structure:
    • Monomer or homodimer.

neXtProt entry for EEF2K Gene

Proteomics data for EEF2K Gene at MOPED

Selected DME Specific Peptides for EEF2K Gene

Post-translational modifications for EEF2K Gene

  • Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.
  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys65, Lys162, Lys238, Lys341, Lys347, Lys485, Lys603, and Lys705

Other Protein References for EEF2K Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Domains for EEF2K Gene

Gene Families for EEF2K Gene

UniProtKB/Swiss-Prot:

EF2K_HUMAN
Domain:
  • The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain.:
    • O00418
  • Contains 1 alpha-type protein kinase domain.:
    • O00418
Family:
  • Belongs to the protein kinase superfamily. Alpha-type protein kinase family.:
    • O00418
genes like me logo Genes that share domains with EEF2K: view

Function for EEF2K Gene

Molecular function for EEF2K Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.
UniProtKB/Swiss-Prot EnzymeRegulation: Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.
UniProtKB/Swiss-Prot Function: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Enzyme Numbers (IUBMB) for EEF2K Gene

Gene Ontology (GO) - Molecular Function for EEF2K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity TAS 9144159
GO:0004674 protein serine/threonine kinase activity --
GO:0004686 elongation factor-2 kinase activity IDA 9144159
GO:0005509 calcium ion binding IEA --
GO:0005516 calmodulin binding IDA 9144159
genes like me logo Genes that share ontologies with EEF2K: view

Phenotypes for EEF2K Gene

MGI mutant phenotypes for EEF2K:
inferred from 1 alleles
GenomeRNAi human phenotypes for EEF2K:
genes like me logo Genes that share phenotypes with EEF2K: view

Animal Models for EEF2K Gene

MGI Knock Outs for EEF2K:

miRNA for EEF2K Gene

miRTarBase miRNAs that target EEF2K

No data available for Transcription Factor Targeting and HOMER Transcription for EEF2K Gene

Localization for EEF2K Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for EEF2K Gene COMPARTMENTS Subcellular localization image for EEF2K gene
Compartment Confidence
cytosol 4
nucleus 2
endoplasmic reticulum 1
extracellular 1
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for EEF2K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm TAS 9144159
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with EEF2K: view

Pathways for EEF2K Gene

genes like me logo Genes that share pathways with EEF2K: view

Gene Ontology (GO) - Biological Process for EEF2K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006414 translational elongation TAS 9144159
GO:0006468 protein phosphorylation --
GO:0008286 insulin receptor signaling pathway TAS --
GO:0031952 regulation of protein autophosphorylation IEA --
GO:0046777 protein autophosphorylation IDA 23184662
genes like me logo Genes that share ontologies with EEF2K: view

Compounds for EEF2K Gene

(5) Tocris Compounds for EEF2K Gene

Compound Action Cas Number
A 484954 CaM kinase III (eEF-2 kinase) inhibitor [142557-61-7]
Arcyriaflavin A Potent cdk4/cyclin D1 and CaM Kinase II inhibitor. Antiviral agent (anti-HCMV) [118458-54-1]
KN-93 CaM kinase II inhibitor. Also K+ channel blocker (KV) [139298-40-1]
NH 125 CaM kinase III (eEF-2 kinase) inhibitor [278603-08-0]
STO-609 acetate Selective CaM kinase kinase inhibitor [52029-86-4]

(1) HMDB Compounds for EEF2K Gene

Compound Synonyms Cas Number PubMed IDs
Calcium
  • Ca
7440-70-2

(14) Novoseek inferred chemical compound relationships for EEF2K Gene

Compound -log(P) Hits PubMed IDs
rottlerin 72.5 5
rapamycin 64.3 8
anisomycin 47.5 5
atp 29.3 5
nmda 23.4 2
genes like me logo Genes that share compounds with EEF2K: view

Transcripts for EEF2K Gene

mRNA/cDNA for EEF2K Gene

Unigene Clusters for EEF2K Gene

Eukaryotic elongation factor-2 kinase:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for EEF2K Gene

No ASD Table

Relevant External Links for EEF2K Gene

GeneLoc Exon Structure for
EEF2K
ECgene alternative splicing isoforms for
EEF2K

Expression for EEF2K Gene

mRNA expression in normal human tissues for EEF2K Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for EEF2K Gene

This gene is overexpressed in Skin - Sun Exposed (Lower leg) (4.1).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for EEF2K Gene

SOURCE GeneReport for Unigene cluster for EEF2K Gene Hs.498892

genes like me logo Genes that share expressions with EEF2K: view

Orthologs for EEF2K Gene

This gene was present in the common ancestor of animals.

Orthologs for EEF2K Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia EEF2K 36
  • 99.36 (n)
  • 99.03 (a)
EEF2K 37
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia EEF2K 36
  • 88.8 (n)
  • 90.87 (a)
EEF2K 37
  • 91 (a)
OneToOne
dog
(Canis familiaris)
Mammalia EEF2K 36
  • 89.56 (n)
  • 93.24 (a)
EEF2K 37
  • 93 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Eef2k 36
  • 85.91 (n)
  • 90.19 (a)
Eef2k 16
Eef2k 37
  • 90 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia EEF2K 37
  • 84 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia EEF2K 37
  • 85 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Eef2k 36
  • 85.73 (n)
  • 90.33 (a)
chicken
(Gallus gallus)
Aves EEF2K 36
  • 74.25 (n)
  • 78.9 (a)
EEF2K 37
  • 79 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia EEF2K 37
  • 71 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.18633 36
tropical clawed frog
(Silurana tropicalis)
Amphibia eef2k 36
  • 69.92 (n)
  • 74.06 (a)
zebrafish
(Danio rerio)
Actinopterygii eef2k 36
  • 66.31 (n)
  • 69.51 (a)
eef2k 37
  • 62 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea efk-1 36
  • 49.28 (n)
  • 45.79 (a)
efk-1 37
  • 36 (a)
OneToMany
efk-1 38
  • 63 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.10624 36
sea squirt
(Ciona savignyi)
Ascidiacea -- 37
  • 44 (a)
ManyToMany
CSA.7709 37
  • 43 (a)
ManyToMany
Species with no ortholog for EEF2K:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for EEF2K Gene

ENSEMBL:
Gene Tree for EEF2K (if available)
TreeFam:
Gene Tree for EEF2K (if available)

Paralogs for EEF2K Gene

Paralogs for EEF2K Gene

genes like me logo Genes that share paralogs with EEF2K: view

Variants for EEF2K Gene

Sequence variations from dbSNP and Humsavar for EEF2K Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type MAF
rs716304 -- 22,260,787(+) CAACA(A/G)TGCGA intron-variant
rs754290 -- 22,212,188(-) TCATC(A/G)TGCAA intron-variant
rs868431 -- 22,220,072(-) ACGTG(A/G)CCACT intron-variant
rs868800 -- 22,240,191(+) CAACA(A/G)GGAGC intron-variant
rs929849 -- 22,210,165(-) TGGGG(A/G)GCACT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for EEF2K Gene

Variant ID Type Subtype PubMed ID
nsv7280 OTHER Inversion 18451855
dgv3n1 OTHER Inversion 15895083
nsv511047 OTHER Inversion 20534489
dgv47n16 OTHER Inversion 17901297
nsv499139 OTHER Inversion 21111241
esv998989 OTHER Inversion 20482838
esv2714070 CNV Deletion 23290073
dgv48n16 CNV Insertion 17901297
nsv1764 CNV Loss 18451855
nsv435668 CNV Deletion 17901297
nsv498860 CNV Loss 21111241
dgv2647n71 CNV Loss 21882294
nsv905598 CNV Gain 21882294
nsv519813 CNV Gain+Loss 19592680
nsv469818 CNV Complex 16826518
esv2662229 CNV Deletion 23128226
dgv834e1 CNV Complex 17122850

Relevant External Links for EEF2K Gene

HapMap Linkage Disequilibrium report
EEF2K
Human Gene Mutation Database (HGMD)
EEF2K

Disorders for EEF2K Gene

(5) Novoseek inferred disease relationships for EEF2K Gene

Disease -log(P) Hits PubMed IDs
glioblastoma 15.4 5
glioma 14.5 2
breast cancer 0 3
tumors 0 3
cancer 0 4

Relevant External Links for EEF2K

Genetic Association Database (GAD)
EEF2K
Human Genome Epidemiology (HuGE) Navigator
EEF2K
genes like me logo Genes that share disorders with EEF2K: view

No data available for UniProtKB/Swiss-Prot for EEF2K Gene

Publications for EEF2K Gene

  1. Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase. (PMID: 9144159) Ryazanov A.G. … Hait W.N. (Proc. Natl. Acad. Sci. U.S.A. 1997) 2 3 4 23
  2. A novel method to identify protein kinase substrates: eEF2 kinase is phosphorylated and inhibited by SAPK4/p38delta. (PMID: 11500363) Knebel A. … Cohen P. (EMBO J. 2001) 3 4 23
  3. Regulation of elongation factor 2 kinase by p90(RSK1) and p70 S6 kinase. (PMID: 11500364) Wang X. … Proud C.G. (EMBO J. 2001) 3 4 23
  4. Stimulation of the AMP-activated protein kinase leads to activation of eukaryotic elongation factor 2 kinase and to its phosphorylation at a novel site, serine 398. (PMID: 14709557) Browne G.J. … Proud C.G. (J. Biol. Chem. 2004) 3 4 23
  5. Mapping the functional domains of elongation factor-2 kinase. (PMID: 11015200) Pavur K.S. … Ryazanov A.G. (Biochemistry 2000) 3 4 23

Products for EEF2K Gene

  • Addgene plasmids for EEF2K

Sources for EEF2K Gene

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