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EEF1A1 Gene

protein-coding   GIFtS: 68
GCID: GC06M074225

Eukaryotic Translation Elongation Factor 1 Alpha 1

(Previous name: leukocyte receptor cluster (LRC) member 7)
(Previous symbols: EF1A, EEF1A, LENG7)
  See EEF1A1-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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This gene clusters with an RNA gene
Subcategory (RNA class): lncRNA

Quality score for the ORGUL clustered with this gene is 3

Aliases
Eukaryotic Translation Elongation Factor 1 Alpha 11 2     EEF-12
EF1A1 2 3 5     GRAF-1EF2
EEF1A1 2 3     HNGC:163032
LENG71 2 3     PTI12
Leukocyte Receptor Cluster (LRC) Member 71 2     Cervical Cancer Suppressor 32
Elongation Factor Tu2 3     CTCL Tumor Antigen2
Eukaryotic Elongation Factor 1 A-12 3     EF1a-Like Protein2
Leukocyte Receptor Cluster Member 72 3     Elongation Factor 1 Alpha Subunit2
EF-1-alpha-12 3     Elongation Factor 1-Alpha 12
EF-Tu2 3     Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 142
eEF1A-12 3     Glucocorticoid Receptor AF-1 Specific Elongation Factor2
CCS-32     Prostate Tumor-Inducing Protein 12
CCS32     Translation Elongation Factor 1 Alpha 1-Like 142
EE1A12     

External Ids:    HGNC: 31891   Entrez Gene: 19152   Ensembl: ENSG000001565087   OMIM: 1305905   UniProtKB: P681043   
ORGUL members:         

Export aliases for EEF1A1 gene to outside databases

Previous GC identifers: GC06M074183 GC06M074221 GC06M074222 GC06M074282


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for EEF1A1 Gene:
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the
enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta,
lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal
muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been
found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
(provided by RefSeq, Jul 2008)

GeneCards Summary for EEF1A1 Gene:
EEF1A1 (eukaryotic translation elongation factor 1 alpha 1) is a protein-coding gene. Diseases associated with EEF1A1 include tinea nigra, and felty's syndrome. GO annotations related to this gene include GTP binding and GTPase activity. An important paralog of this gene is EEF1A2.

UniProtKB/Swiss-Prot: EF1A1_HUMAN, P68104
Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during
protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific
transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus
involved importantly in Th1 cytokine production

Gene Wiki entry for EEF1A1 (Eukaryotic translation elongation factor 1 alpha 1) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000006.11  NC_018917.2  NT_025741.16  
Regulatory elements:
   Regulatory transcription factor binding sites in the EEF1A1 gene promoter:
         SRF   NF-1   SRF (504 AA)   Pax-2   Pax-2a   Tal-1beta   HSF2   ITF-2   Pax-4a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): EEF1A1 promoter sequence
   Search Chromatin IP Primers for EEF1A1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat EEF1A1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6q14.1   Ensembl cytogenetic band:  6q13   HGNC cytogenetic band: 6q14.1

EEF1A1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
EEF1A1 gene location

GeneLoc information about chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06M074225:  view genomic region     (about GC identifiers)

Start:
74,225,473 bp from pter      End:
74,233,520 bp from pter
Size:
8,048 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: EF1A1_HUMAN, P68104 (See protein sequence)
Recommended Name: Elongation factor 1-alpha 1  
Size: 462 amino acids; 50141 Da
Subunit: Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1,
TXK, XPO5 and KARS. May interact with ERGIC2. Interacts with IFIT1 (via TPR repeats 4-7) (By similarity).
Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor
stimulation
Sequence caution: Sequence=AAA52367.1; Type=Erroneous initiation;
1 PDB 3D structure from and Proteopedia for EEF1A1:
1SYW (3D)    
Secondary accessions: P04719 P04720

Explore the universe of human proteins at neXtProt for EEF1A1: NX_P68104

Explore proteomics data for EEF1A1 at MOPED

Post-translational modifications: 

  • ISGylated1
  • Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency1
  • Ubiquitination2 at Lys20, Lys30, Lys41, Lys44, Lys62, Lys146, Lys154, Lys165, Lys172, Lys179,
                                 Lys180, Lys212, Lys255, Lys273, Lys318, Lys392, Lys395, Lys408, Lys439
  • Glycosylation2 at Asn284
  • Modification sites at PhosphoSitePlus

  • See EEF1A1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_001393.1  
    ENSEMBL proteins: 
     ENSP00000339063   ENSP00000339053   ENSP00000330054   ENSP00000348651   ENSP00000392366  
    Reactome Protein details: P68104

    EEF1A1 Human Recombinant Protein Products:

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    Novus Biologicals EEF1A1 Proteins
    Novus Biologicals EEF1A1 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
    Search eBioscience for Proteins for EEF1A1 

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    Abcam antibodies for EEF1A1
    Browse Antibodies at Cloud-Clone Corp.
    ThermoFisher Antibodies for EEF1A1
    LSBio Antibodies in human, mouse, rat for EEF1A1

    EEF1A1 Assay Products:

    EMD Millipore Kits and Assays for the Analysis of EEF1A1
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    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for EEF1A1 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    Selected InterPro protein domains (see all 7):
     IPR027417 P-loop_NTPase
     IPR009001 Transl_elong_EF1A/Init_IF2_C
     IPR000795 EF_GTP-bd_dom
     IPR004160 Transl_elong_EFTu/EF1A_C
     IPR004161 Transl_elong_EFTu/EF1A_2

    Graphical View of Domain Structure for InterPro Entry P68104

    ProtoNet protein and cluster: P68104

    UniProtKB/Swiss-Prot: EF1A1_HUMAN, P68104
    Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily


    Find genes that share domains with EEF1A1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: EF1A1_HUMAN, P68104
    Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during
    protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific
    transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus
    involved importantly in Th1 cytokine production
    Induction: By homocysteine (HC), may mediate accelerated synthesis of free thiol-containing proteins in response
    to HC-induced oxidative stress

         Genatlas biochemistry entry for EEF1A1:
    eukaryotic translation elongation factor 1,alpha 1,ubiquitously expressed

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003746translation elongation factor activity TAS17595531
    GO:0003924GTPase activity IEA--
    GO:0005515protein binding IPI15161933
    GO:0005525GTP binding TAS3512269
    GO:0019901protein kinase binding IPI17595531
         
    Find genes that share ontologies with EEF1A1           About GenesLikeMe


    Phenotypes:
         6 GenomeRNAi human phenotypes for EEF1A1:
     Decreased influenza A H1N1 (A/  Decreased influenza A/WSN/33 r  Decreased melanin production  Increased G1 DNA content 
     Increased cell death in HCC-19  Synthetic lethal with Ras 

         5 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Eef1a1):
     hematopoietic system  immune system  mortality/aging  no phenotypic analysis  tumorigenesis 

    Find genes that share phenotypes with EEF1A1           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for EEF1A1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for EEF1A1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for EEF1A1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for EEF1A1

    miRNA
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    miRTarBase miRNAs that target EEF1A1:
    hsa-mir-92a-3p (MIRT049131), hsa-mir-93-3p (MIRT038724), hsa-mir-197-3p (MIRT048051), hsa-mir-17-5p (MIRT050841), hsa-let-7g-5p (MIRT046556), hsa-mir-324-3p (MIRT042916), hsa-mir-421 (MIRT039430), hsa-mir-221-3p (MIRT046863), hsa-mir-100-5p (MIRT048484), hsa-mir-1226-3p (MIRT036484), hsa-mir-125a-5p (MIRT045725), hsa-mir-30e-5p (MIRT044159), hsa-mir-484 (MIRT041803), hsa-mir-615-3p (MIRT040041), hsa-mir-30c-5p (MIRT047870), hsa-mir-652-3p (MIRT039465), hsa-mir-339-5p (MIRT042754), hsa-mir-1260b (MIRT052768), hsa-mir-26b-5p (MIRT050042), hsa-mir-501-5p (MIRT041144), hsa-mir-18a-5p (MIRT050679), hsa-mir-505-3p (MIRT041069), hsa-mir-24-3p (MIRT050378), hsa-mir-497-5p (MIRT041183), hsa-mir-185-5p (MIRT045351), hsa-mir-15b-5p (MIRT046401), hsa-mir-96-5p (MIRT048716), hsa-mir-16-5p (MIRT031618), hsa-mir-106b-5p (MIRT044291), hsa-mir-125b-5p (MIRT045935), hsa-mir-93-5p (MIRT048759), hsa-mir-361-3p (MIRT038270), hsa-mir-186-5p (MIRT044909), hsa-mir-877-5p (MIRT037373), hsa-mir-423-3p (MIRT042476), hsa-mir-340-3p (MIRT043857), hsa-mir-30a-5p (MIRT049950), hsa-let-7b-5p (MIRT051968), hsa-mir-23b-3p (MIRT046331), hsa-mir-25-3p (MIRT050247), hsa-mir-455-3p (MIRT037887), hsa-mir-296-3p (MIRT038433)

    Block miRNA regulation of human, mouse, rat EEF1A1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate EEF1A1 (see all 42):
    hsa-miR-548k hsa-miR-371-5p hsa-miR-133a hsa-miR-3613-3p hsa-miR-29a* hsa-miR-548am hsa-miR-1237 hsa-miR-548e
    SwitchGear 3'UTR luciferase reporter plasmidEEF1A1 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat EEF1A1

    Gene Editing
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    GenScript: all cDNA clones in your preferred vector: EEF1A1 (NM_001402)
    Sino Biological Human cDNA Clone for EEF1A1
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for EEF1A1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat EEF1A1
    Addgene plasmids for EEF1A1 

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for EEF1A1

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    eBioscience FlowRNA Probe Sets ( VA1-10418) for EEF1A1 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    EF1A1_HUMAN, P68104: Cytoplasm. Nucleus. Note=Colocalizes with DLC1 at actin-rich regions in the cell periphery
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol4
    chloroplast2
    cytoskeleton2
    mitochondrion2
    plasma membrane1

    Gene Ontology (GO): Selected cellular component terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005615extracellular space IDA--
    GO:0005634nucleus IDA9852145
    GO:0005730nucleolus IDA9852145
    GO:0005737cytoplasm TAS3512269
    GO:0005829cytosol TAS--

    Find genes that share ontologies with EEF1A1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for EEF1A1 About   (see all 8)  
    See pathways by source

    SuperPathContained pathways About
    1Influenza Viral RNA Transcription and Replication
    Peptide chain elongation0.94
    Translation0.82
    Eukaryotic Translation Elongation0.93
    2Gene Expression
    Gene Expression0.40
    3Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
    Metabolism of proteins0.30
    4Translational Control
    Translational Control
    5Translation Factors
    Translation Factors


    Find genes that share SuperPaths with EEF1A1           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for EEF1A1
        Translational Control

    2 BioSystems Pathways for EEF1A1
        EGFR1 Signaling Pathway
    Translation Factors


    3 Reactome Pathways for EEF1A1
        Peptide chain elongation
    Eukaryotic Translation Elongation
    HSF1 activation


    2 Kegg Pathways  (Kegg details for EEF1A1):
        RNA transport
    Legionellosis

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for EEF1A1
    Interactions:

        GeneGlobe Interaction Network for EEF1A1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for EEF1A1 (P681041, 2, 3 ENSP000003300544) via UniProtKB, MINT, STRING, and/or I2D (see all 1676)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ILF2Q129052, ENSP000003550114MINT-6489539 MINT-6488590 MINT-7947479 MINT-6488791 MINT-7945693 MINT-6489355 MINT-8076347 MINT-8076071 MINT-8076236 STRING: ENSP00000355011
    YWHAZP631041, 2, 3, ENSP000003095034EBI-352162,EBI-347088 MINT-3297472 MINT-7900157 I2D: score=1 STRING: ENSP00000309503
    RUVBL2Q9Y2302, 3MINT-6489217 MINT-7947479 MINT-7945693 MINT-6489355 MINT-6488488 MINT-8076347 MINT-8076071 MINT-8076236 I2D: score=1 
    SF3B3Q153932, 3MINT-6489539 MINT-6488590 MINT-7947479 MINT-6488791 MINT-6542674 MINT-7945693 MINT-6489355 I2D: score=1 
    RPS15AP622442, 3, ENSP000003186464MINT-6489539 MINT-6488590 MINT-6488791 MINT-6542674 MINT-6489355 I2D: score=3 STRING: ENSP00000318646
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-templated IEA--
    GO:0006355regulation of transcription, DNA-templated IEA--
    GO:0006412translation TAS--
    GO:0006414translational elongation TAS8812466
    GO:0010467gene expression TAS--

    Find genes that share ontologies with EEF1A1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for EEF1A1 (EF1A1)

    4 HMDB Compounds for EEF1A1    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine diphosphate5'-GDP (see all 10)146-91-8--
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--
    PhosphateNFB Orthophosphate (see all 13)14265-44-2--
    WaterDihydrogen oxide (see all 2)7732-18-5--

    1 DrugBank Compound for EEF1A1    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Guanosine-5'-Diphosphate-- 146-91-8target--10592235

    Selected Novoseek inferred chemical compound relationships for EEF1A1 gene (see all 59)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    kirromycin 90.6 12 9405422 (2), 12370016 (1), 2207147 (1), 10588048 (1) (see all 9)
    aminoacyl-trna 90.2 99 19833920 (5), 2011527 (3), 12379845 (3), 2110000 (3) (see all 46)
    aurodox 83.6 8 2110000 (3), 12370016 (1), 7851417 (1)
    gtp 82.7 165 18773979 (4), 2011527 (3), 15952884 (3), 2157708 (3) (see all 79)
    gdp 82 59 2110000 (3), 8665868 (2), 18773979 (2), 8722020 (2) (see all 38)
    ge2270a 78.5 3 8494369 (1), 9209782 (1), 12370016 (1)
    pulvomycin 77.4 5 15581367 (3), 9405422 (2)
    trna 76.4 42 18336835 (3), 18221514 (2), 15952884 (2), 12354112 (2) (see all 27)
    smpb 74.5 9 16601123 (4), 11904186 (1), 11694632 (1), 16381838 (1)
    xanthosine 5'-triphosphate 67.5 1 8446899 (1)



    Find genes that share compounds with EEF1A1           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for EEF1A1 gene (2 alternative transcripts): 
    NM_001402.5  NM_001403.1  

    Unigene Clusters for EEF1A1:

    Eukaryotic translation elongation factor 1 alpha 1
    Hs.535192  [show with all ESTs], Hs.586423  [show with all ESTs]
    Unigene Representative Sequences: NM_001402, BC001412
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000316292(uc003phi.3 uc003phj.3 uc021zbs.1) ENST00000491404
    ENST00000309268(uc003phl.3 uc003phm.1) ENST00000490569 ENST00000331523
    ENST00000495333 ENST00000488500 ENST00000356303 ENST00000455918
    miRNA
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    Block miRNA regulation of human, mouse, rat EEF1A1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate EEF1A1 (see all 42):
    hsa-miR-548k hsa-miR-371-5p hsa-miR-133a hsa-miR-3613-3p hsa-miR-29a* hsa-miR-548am hsa-miR-1237 hsa-miR-548e
    SwitchGear 3'UTR luciferase reporter plasmidEEF1A1 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat EEF1A1
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    GenScript: all cDNA clones in your preferred vector: EEF1A1 (NM_001402)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for EEF1A1
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    Addgene plasmids for EEF1A1 
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    Flow Cytometry
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    eBioscience FlowRNA Probe Sets ( VA1-10418) for EEF1A1 

    Additional mRNA sequence: 

    AF174496.1 AF328730.1 AK024888.1 AK026650.1 AK055058.1 AK058163.1 AK098510.1 AK098518.1 
    AK098587.1 AK098623.1 AK127384.1 AK222515.1 AK222519.1 AK222523.1 AK222551.1 AK222982.1 
    AK223030.1 AK223042.1 AK223046.1 AK289374.1 AK289442.1 AK290691.1 AK292639.1 AK315827.1 
    BC001412.1 BC002845.1 BC006102.1 BC008587.1 BC009733.1 BC009875.2 BC010735.1 BC012509.1 
    BC012891.1 BC014224.2 BC014377.1 BC014892.1 BC018150.1 BC018641.2 BC018850.2 BC019050.2 
    BC020477.2 BC021686.1 BC022412.1 BC028674.1 BC029337.1 BC029343.1 BC029997.1 BC035877.1 
    BC038339.1 BC057391.1 BC063511.1 BC065761.1 BC066893.1 BC066894.1 BC070131.1 BC071727.1 
    BC071741.1 BC071841.1 BC072385.1 BC082268.1 BC111051.1 EF219140.1 EF362804.1 M29548.1 
    X03558.1 X16869.1 

    Selected DOTS entries (see all 1167):

    DT.100894282  DT.100748056  DT.92071614  DT.92071332  DT.100893975  DT.100770577  DT.91892031  DT.100894339 
    DT.101962924  DT.100894535  DT.100894180  DT.85101477  DT.100747923  DT.100894204  DT.91994360  DT.92342128 
    DT.95377109  DT.95377203  DT.100894067  DT.97870655  DT.95376680  DT.100857960  DT.100894270  DT.100894582 

    Selected AceView cDNA sequences (see all 2606):

    BX427169 BC082268 CR626524 AA341431 CR608408 CR608952 AA190937 CR607024 
    CR604567 CR611904 CR609969 CR603064 CR595015 L13815 BQ774922 CR604465 
    CR621775 CR593746 CR591058 BX387535 CR595294 CR598223 CR607264 CR591544 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for EEF1A1    About this scheme

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6
    SP1:                                          
    SP2:                                          
    SP3:                                          


    ECgene alternative splicing isoforms for EEF1A1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    EEF1A1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TGTGTTGAGA
    EEF1A1 Expression
    About this image


    EEF1A1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 22) fully expand
     
     Brain (Nervous System)    fully expand to see all 21 entries
             Thalamus
             Septum   
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Liver (Hepatobiliary System)
             Hepatocytes Liver Lobule
     
     Ovary (Reproductive System)
             Oviduct
     
     Pancreas (Endocrine System)
             Islets of Langerhans
    EEF1A1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    EEF1A1 Protein Expression

    SOURCE GeneReport for Unigene clusters: Hs.535192 Hs.586423

    UniProtKB/Swiss-Prot: EF1A1_HUMAN, P68104
    Tissue specificity: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal
    muscle

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for EEF1A1 gene from Selected species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Eef1a11 , 5 eukaryotic translation elongation factor 1 alpha 11, 5 91.85(n)1
    99.78(a)1
      9 (43.65 cM)5
    136271  NM_010106.21  NP_034236.21 
     784784495 
    chicken
    (Gallus gallus)
    Aves EEF1A11 eukaryotic translation elongation factor 1 alpha 1 86.29(n)
    99.13(a)
      373963  NM_204157.2  NP_989488.2 
    lizard
    (Anolis carolinensis)
    Reptilia EEF1A16
    eukaryotic translation elongation factor 1 alpha 1...
    99(a)
    1 ↔ 1
    GL343341.1(112716-119283)
    African clawed frog
    (Xenopus laevis)
    Amphibia BC041196.12   -- 85.88(n)    BC041196.1 
    zebrafish
    (Danio rerio)
    Actinopterygii BC060907.12   -- 84.65(n)   336334  BC060907.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Ef1alpha48D3 translational elongation translation
    elongation more
    85(a)
    (best of 2)
      48D1   --
    worm
    (Caenorhabditis elegans)
    Secernentea eft-33 Elongation factor 1-alpha 83(a)   III(7041894-7043435)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes TEF1(YPR080W)4 Translational elongation factor EF-1 alpha; also encoded more   --   16(700594-701970) 856195  NP_015405.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT1G079401 AT1G07940 71.99(n)
    77.98(a)
      837309  NM_001035916.2  NP_001030993.1 
    rice
    (Oryza sativa)
    Liliopsida Os03g01775001 Os03g0177500 71.66(n)
    79.19(a)
      4331811  NM_001055681.1  NP_001049146.1 


    ENSEMBL Gene Tree for EEF1A1 (if available)
    TreeFam Gene Tree for EEF1A1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

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    Paralogs for EEF1A1 gene
    EEF1A22  
    11 SIMAP similar genes for EEF1A1 using alignment to 13 protein entries:     EF1A1_HUMAN (see all proteins):
    EEF1A1P5    EEF1A1L14    PTI-1    EEF1A2    EEF1A    HBS1L
    GSPT1    GSPT2    GFM1    GFM2    GUF1

    Find genes that share paralogs with EEF1A1           About GenesLikeMe


    Selected Pseudogenes.org Pseudogenes for EEF1A1 (see all 34)
    PGOHUM00000238385 PGOHUM00000242326 PGOHUM00000239553 PGOHUM00000239691 PGOHUM00000239752


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

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    Selected SNPs for EEF1A1 (see all 246)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 6 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs121111431,2
    C--74224991(+) GTCAGT/CGTAAA 1 -- ds50014Minor allele frequency- C:0.10NA WA EA 242
    rs1820250321,2
    --74225103(+) CTGCCA/GAGGCC 1 -- ds50010--------
    rs1509229241,2
    --74225131(+) TAAAAA/GATAAA 1 -- ds50010--------
    rs77513421,2
    C,F,A,H--74225145(+) CAGATT/GAACTG 1 -- ds500122Minor allele frequency- G:0.45NS EA NA WA CSA 2348
    rs1394751371,2
    C--74225152(+) ACTGGG/TAAAGA 1 -- ds50010--------
    rs1169146581,2
    C,F--74225169(+) ACAGGC/AAGATC 1 -- ds50011Minor allele frequency- A:0.02EA 120
    rs1854062251,2
    --74225186(+) GCCCAA/GGAGTT 1 -- ds50010--------
    rs1890296041,2
    --74225230(+) TTAGCC/GAGGCG 1 -- ds50010--------
    rs1497337971,2
    --74225235(+) CAGGCA/GTGGTG 1 -- ds50010--------
    rs37571131,2
    C,F,H--74225333(+) CAGGGC/AAGGAG 1 -- ds50017Minor allele frequency- A:0.01NS EA 862

    HapMap Linkage Disequilibrium report for EEF1A1 (74225473 - 74233520 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for EEF1A1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2675873CNV Deletion23128226
    nsv886159CNV Loss21882294
    nsv5350CNV Loss18451855
    nsv463153CNV Loss19166990
    nsv886158CNV Gain21882294

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing EEF1A1
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

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    OMIM gene information: 130590    OMIM disorders: --

    3 diseases for EEF1A1:    
    About MalaCards
    tinea nigra    felty's syndrome    cervical cancer

    1 disease from the University of Copenhagen DISEASES database for EEF1A1:
    Tinea nigra

    Find genes that share disorders with EEF1A1           About GenesLikeMe

    Selected Novoseek inferred disease relationships for EEF1A1 gene (see all 17)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    mimicry 40.5 3 12370017 (1), 15317456 (1)
    sarcoma rous 13.5 2 16099414 (1), 9099894 (1)
    prostate carcinoma 13.3 15 16707856 (4), 8988032 (3), 7542776 (3), 9465091 (1) (see all 5)
    shock 12.7 12 12417652 (1), 19523215 (1), 12641431 (1), 12747404 (1) (see all 11)
    necrosis 4.02 2 18688046 (1), 19856081 (1)
    leukemia 0 3 16099414 (1), 18388914 (1), 17609656 (1), 11082319 (1)
    vesicular stomatitis 0 1 12529558 (1)
    hepatocellular carcinoma 0 1 17825975 (1)
    colon carcinoma 0 2 8988032 (1), 10216286 (1)
    benign prostatic hyperplasia 0 3 16707856 (1), 8988032 (1), 7542776 (1)

    Genetic Association Database (GAD): EEF1A1

    Export disorders for EEF1A1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

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    PubMed articles for EEF1A1 gene, integrated from 10 sources (see all 485):
    (articles sorted by number of sources associating them with EEF1A1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. (PubMed id 12426392)1, 2, 9 Bohnsack M.T.... Goerlich D. (EMBO J. 2002)
    2. Phosphorylation of eEF1A1 at Ser300 by TbetaR-I results in inhibition of mRNA translation. (PubMed id 20832312)1, 2 Lin K.W.... Souchelnytskyi S. (Curr. Biol. 2010)
    3. The SAM domain of the RhoGAP DLC1 binds EF1A1 to regulate cell migration. (PubMed id 19158340)1, 2 Zhong D.... Low B.C. (J. Cell Sci. 2009)
    4. Male germ cell expression of the PAS domain kinase PASKIN and its novel target eukaryotic translation elongation factor eEF1A1. (PubMed id 17595531)1, 2 Eckhardt K.... Wenger R.H. (Cell. Physiol. Biochem. 2007)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    6. Exportin-5-mediated nuclear export of eukaryotic elongation factor 1A and tRNA. (PubMed id 12426393)1, 2 Calado A.... Kutay U. (EMBO J. 2002)
    7. Extensive gene duplications and a large inversion characterize the human leukocyte receptor cluster. (PubMed id 10941842)1, 3 Wende H.... Ziegler A. (Immunogenetics 2000)
    8. Assignment of human elongation factor 1alpha genes: EEF1A maps to chromosome 6q14 and EEF1A2 to 20q13.3. (PubMed id 8812466)1, 3 Lund A.... Tommerup N. (Genomics 1996)
    9. Retropseudogenes constitute the major part of the human elongation factor 1 alpha gene family. (PubMed id 2183196)1, 2 Madsen H.O.... Hjorth J.P. (Nucleic Acids Res. 1990)
    10. Murine elongation factor 1 alpha (EF-1 alpha) is posttranslationally modified by novel amide-linked ethanolamine-phosphoglycerol moieties. Addition of ethanolamine-phosphoglycerol to specific glutamic acid residues on EF-1 alpha. (PubMed id 2569467)1, 2 Whiteheart S.W.... Hart G.W. (J. Biol. Chem. 1989)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

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    Entrez Gene: 1915 HGNC: 3189 AceView: EEF1A1 Ensembl:ENSG00000156508 euGenes: HUgn1915
    ECgene: EEF1A1 Kegg: 1915 H-InvDB: EEF1A1

    (According to HUGE)
    About This Section

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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for EEF1A1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for EEF1A1 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for EEF1A1 gene:
    Search GeneIP for patents involving EEF1A1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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