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EDEM2 Gene

protein-coding   GIFtS: 54

GC20M033167
ER degradation enhancer, mannosidase alpha-like 2
(Previous name: chromosome 20 open reading frame 31 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: C20orf49, C20orf31)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
C20orf31 2, 3
C20orf49 2, 3
FLJ10783 1, 2
OTTHUMP00000030728 2
bA4204.1 1, 2
Descriptions
ER degradation enhancer, mannosidase alpha-like 2 2
ER degradation-enhancing-mannosidase-like protein 2 2
chromosome 20 open reading frame 31 1
External Ids
HGNC: 158771
Entrez Gene: 557412
UniProtKB: Q9BV943
Ensembl: ENSG000000882987
Search outside databases for aliases for EDEM2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for EDEM2:
In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and
degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs
to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed
15537790]).[supplied by OMIM]

Gene Wiki entry for EDEM2
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the EDEM2 gene  

Entrez Gene cytogenetic band: 20q11.22   Ensembl cytogenetic band:  20q11.22   HGNC cytogenetic band: 20q11.22

EDEM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M033167:     (about GC identifiers)

Start:
33,166,831 bp from pter
End:
33,198,828 bp from pter
Size:
31,998 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000020.9  NT_028392.5  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: EDEM2_HUMAN, Q9BV94 (See protein sequence)
Recommended Name: ER degradation-enhancing alpha-mannosidase-like 2 precursor  
Size: 578 amino acids; 64753 Da
Subcellular location: Secreted (Potential)
Secondary accessions: Q6GU33 Q6IA89 Q6UWZ4 Q9H4U0 Q9H886 Q9NTL9 Q9NVE6
Alternative splicing: 2 isoforms:  Q9BV94-1   Q9BV94-2   

REFSEQ proteins (2 alternative transcripts): 
NP_001138497.1  NP_060687.2  


ENSEMBL proteins: 
ENSP00000363616 ENSP00000363615 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (C20orf31)
Human Recombinant Proteins from Abnova (EDEM2)
              OriGene Purified Recombinant Human Protein: EDEM2 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005576 extracellular region IEA--
GO:0016020 membrane IEA--
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Antibodies for EDEM2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for EDEM2
Browse R&D Systems for Antibodies
Antibodies from Abcam (C20orf31), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (EDEM2)
Novus Biologicals Antibodies for EDEM2

Assays for EDEM2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR001382 Glyco_hydro_47


   GeneDecks  EDEM2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9BV94

ProtoNet protein and cluster: Q9BV94

1 Blocks protein family: IPB001382 Glycosyl hydrolase family 47 signature

UniProtKB/Swiss-Prot: EDEM2_HUMAN, Q9BV94
Similarity: Belongs to the glycosyl hydrolase 47 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EDEM2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EDEM2)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NR_026728

              Applied Biosystems Silencer® siRNAs for EDEM2

              Sigma-Aldrich siRNA for EDEM2  
                     Sigma-Aldrich shRNA for EDEM2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for EDEM2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001145025
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001145025
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001145025 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_018217

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity IEA--
GO:0005509 calcium ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  EDEM2  (Your Favorite Gene powered by Ingenuity) 
 N-Glycan Biosynthesis

   GeneDecks  EDEM2 for the pathways selected above  
About GeneDecksing


5 Interacting proteins for EDEM2 (Q9BV942 ENSP000003636163) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
RPS3AP612472MINT-63932
RPS3AP47P612472MINT-63932
RPS3AP49P612472MINT-63932
XBP1ENSP000003431553STRING (score=.834)
CANXENSP000002474613STRING (score=.819)
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for EDEM2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EDEM2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EDEM2)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NR_026728

              Sigma-Aldrich siRNA for EDEM2  
                     Sigma-Aldrich shRNA for EDEM2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_018217  

REFSEQ mRNAs for EDEM2 gene (2 alternative transcripts): 

NM_001145025.1   NM_018217.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_018217  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001145025
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001145025
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001145025 

Additional cDNA sequence: 

AF086313.1 AK001645.1 AK023931.1 AK096016.1 AK296878.1 AK298794.1 AK300212.1 AY007088.1 
AY358580.1 BC001371.2 BC016184.1 BC103840.1 BC133653.1 CR457266.1 CR590035.1 CR591753.1 
CR593253.1 CR594587.1 CR598404.1 CR600513.1 CR601922.1 CR602215.1 CR610779.1 CR612837.1 
CR615992.1 CR624527.1 CR625751.1 XM_001130700.1 

24/37 DOTS entries (see all 37 ):

DT.445883  DT.100703523  DT.100720205  DT.447267  DT.95280269  DT.120830406  DT.92441802  DT.100820856 
DT.120830342  DT.120830494  DT.100844454  DT.91969069  DT.95074569  DT.97845990  DT.95146194  DT.120830318 
DT.100844459  DT.91737497  DT.120830416  DT.65287049  DT.100038418  DT.100844462  DT.100844469  DT.120830519 

24/367 AceView cDNA sequences (see all 367 ):

BQ933418 BQ058216 CA487605 BM822970 BI829386 BQ642190 BM842053 BM773355 
BQ893922 AU141822 BC016184 CR600513 CR602215 BQ222086 AK023931 BQ061510 
BU149362 BQ057954 BU931330 BQ935352 BQ061766 BU526886 BI824035 BU174185 

highest scoring ESTs for EDEM2:

AI364618 BU148537 AA321441 AI421662 AW166086 BF183479 BG015984 BX331549 R59996 AL703210 

Unigene Cluster for EDEM2:

ER degradation enhancer, mannosidase alpha-like 2
Hs.356273  [show with all ESTs]
Unigene Representative Sequence: AK296878


GeneLoc Exon Structure

5/12 Alternative Splicing Database (ASD) splice patterns (SP) for EDEM2 (see all 12 )

ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9a · 9b · 9c ^ 10a · 10b · 10c ^ 11 ^ 12
SP1:                    -     -           -                                   -                                                                     
SP2:                    -     -     -     -                                   -                                                                     
SP3:                                      -     -                             -                                                                     
SP4:                    -     -           -                                                                                                         
SP5:                    -     -           -                             -     -     -     -     -                                                   

About this scheme

ECgene alternative splicing isoforms for EDEM2

2 Ensembl transcripts including schematic representations:
ENST00000374492  ENST00000374491  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

EDEM2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for EDEM2

1 / 2 / 3

3 probe-sets matching EDEM2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
55652_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI191318 0.60 0.60 0.60 1

218282_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018217 0.40 0.67 0.55 1

218282_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  EDEM2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: ACCAGGCAAG

SOURCE GeneReport for Unigene cluster: Hs.356273
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for EDEM2 gene from 5/11 species (see all 11 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
EDEM21   -- ER degradation enhancer, mannosidase alpha-like 2 91.91(n)
96.53(a)
477209  XM_534400.2  XP_534400.1 
chimpanzee
(Pan troglodytes)
EDEM21   -- ER degradation enhancer, mannosidase alpha-like 2 99.37(n)
99.31(a)
458197  XM_514601.2  XP_514601.1 
cow
(Bos taurus)
EDEM21   -- ER degradation enhancer, mannosidase alpha-like 2 91.41(n)
96.19(a)
513253  XM_881672.2  XP_886765.2 
rat
(Rattus norvegicus)
Edem21   -- ER degradation enhancer, mannosidase alpha-like 2 89.64(n)
94.44(a)
296304  NM_001004230.1  NP_001004230.1 
mouse
(Mus musculus)
Edem21, 5 25
ER degradation enhancer, mannosidase alpha-like 21, 5 88.77(n)1
94.1(a)1
1086871  NM_145537.11  NP_663512.11 
 AI3273545  AK0291645  (see all 16)
About this table        Species with no ortholog for EDEM2

ENSEMBL Gene Tree for EDEM2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for EDEM2 gene
EDEM12  EDEM32  

Pseudogenes
1 related pseudogene


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/261 NCBI SNPs in EDEM2 are shown (see all 261 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 69)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 20 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs37464291,2
A,C,F,H33167268(-) TCGACA/GCGGTG 1 T/A mis118Minor allele frequency- N:0.00EA NA EU WA 2436
--
rs11245111,2
C,F,H33200358(-) tccagT/Ggttgg 1 -- ng314Minor allele frequency- G:0.45EU EA WA 418
rs170922971,2
C,F,H33166795(+) CATGAG/AACAGC 1 -- ng5111Minor allele frequency- A:0.22NA EU EA WA 980
rs60602481,2
C,F,H33167105(+) TGCTCC/TGTTTG 1 R/Q mis1 ese38Minor allele frequency- T:0.03EU EA WA 840
rs60602701,2
C,F,H33200475(+) atgagG/Tagttt 1 -- ng318Minor allele frequency- T:0.17EU EA WA 788
rs170923781,2
C,F,H33199849(+) CTTTAC/TCCCTG 1 -- ng317Minor allele frequency- T:0.28NA EU EA WA 550
--
rs10520561,2
C,F33166968(-) CACTTC/TTCAGC 1 L/F mis1 ese34Minor allele frequency- T:0.01EU EA WA 420
--
rs115415031,2
H33198346(-) GAATGC/TCTTTC 1 A/V mis1 trp34Minor allele frequency- T:0.00EU EA WA 406
--
rs61208501,2
H33198289(+) ACTACC/ATGCCC 1 I/S mis14Minor allele frequency- A:0.00EU EA WA 416
rs72620721,2
C,F,H33170170(+) ATTGCG/ACTTTC 1 S/S syn1 ese311Minor allele frequency- A:0.13EU EA WA NA 980
About this table

HapMap Linkage Disequilibrium images for EDEM2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 610302

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/11 PubMed articles for EDEM2 gene (see all 11 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 55741 HGNC: 15877 AceView: C20orf31 Ensembl:ENSG00000088298 euGenes: HUgn55741
ECgene: EDEM2 H-InvDB: EDEM2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for EDEM2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for EDEM2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for EDEM2 Antibodies for EDEM2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for EDEM2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

 Recombinant Proteins (EDEM2)
 Antibodies (EDEM2)
 Chimera RNAi (EDEM2)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
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 Recombinant Proteins
(C20orf31)
 Antibodies (C20orf31)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for EDEM2




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for EDEM2

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