V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

EDEM1 Gene

protein-coding   GIFtS: 56

GC03P005203
ER degradation enhancer, mannosidase alpha-like 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EDEM 1, 2, 3
FLJ51559 2
FLJ51560 2
KIAA0212 1, 2, 3
Descriptions
ER degradation enhancer, mannosidase alpha-like 1 2
ER degradation enhancing alpha mannosidase-like 2
External Ids
HGNC: 189671
Entrez Gene: 96952
UniProtKB: Q926113
Ensembl: ENSG000001341097
Search outside databases for aliases for EDEM1 gene

Previous GC identifers: GC03P005221 GC03P005204

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: EDEM1_HUMAN, Q92611
Function: Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding,
from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation
(ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. It
lacks mannosidase activity

Gene Wiki entry for EDEM1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the EDEM1 gene  

Entrez Gene cytogenetic band: 3p26.2   Ensembl cytogenetic band:  3p26.2   HGNC cytogenetic band: 3p26.1

EDEM1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P005203:     (about GC identifiers)

Start:
5,204,433 bp from pter
End:
5,236,642 bp from pter
Size:
32,210 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_022517.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: EDEM1_HUMAN, Q92611 (See protein sequence)
Recommended Name: ER degradation-enhancing alpha-mannosidase-like 1  
Size: 657 amino acids; 73768 Da
Subunit: Interacts with DERL2 and DERL3
Subcellular location: Endoplasmic reticulum membrane; Single-pass type II membrane protein
(Potential)

REFSEQ proteins: NP_055489.1  

ENSEMBL proteins: 
ENSP00000256497 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (EDEM1)
Human Recombinant Proteins from Abnova (EDEM1)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

5 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005783 endoplasmic reticulum IEA--
GO:0005789 endoplasmic reticulum membrane IEA--
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
GO:0030176 integral to endoplasmic reticulum membrane ISS--
About this table

Antibodies for EDEM1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for EDEM1
Browse R&D Systems for Antibodies
Antibodies from Abcam (EDEM1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (EDEM1)
Novus Biologicals Antibody for EDEM1

Assays for EDEM1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

1 InterPro domain/family:
 IPR001382 Glyco_hydro_47


   GeneDecks  EDEM1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q92611

ProtoNet protein and cluster: Q92611

1 Blocks protein family: IPB001382 Glycosyl hydrolase family 47 signature

UniProtKB/Swiss-Prot: EDEM1_HUMAN, Q92611
Similarity: Belongs to the glycosyl hydrolase 47 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EDEM1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EDEM1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014674

              Applied Biosystems Silencer® siRNAs for EDEM1

              Sigma-Aldrich siRNA for EDEM1  
                     Sigma-Aldrich shRNA for EDEM1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for EDEM1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014674
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014674
                                 untagged cDNA clone in CMV expression vector: NM_014674 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_014674

UniProtKB/Swiss-Prot: EDEM1_HUMAN, Q92611
Function: Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding,
from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation
(ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. It
lacks mannosidase activity

1 MGI phenotypic allele for Edem1 (no phenotypes)


3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity IEA--
GO:0005509 calcium ion binding IEA--
GO:0005515 protein binding IPI16449189
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Sigma-Aldrich "Your Favorite Gene" Pathway for  EDEM1  (Your Favorite Gene powered by Ingenuity) 
 N-Glycan Degradation

   GeneDecks  EDEM1 for the pathways selected above  
About GeneDecksing

 Gene Network CentralTM Interacting Genes and Proteins Network for  EDEM1 


5/13 Interacting proteins for EDEM1 (ENSP000002564973 Q926111, 2) via UniProtKB, MINT, and/or STRING (see all 13 )
InteractantInteraction Details
GeneCardExternal ID(s)
BZW1Q9BUY01EBI-1056336, EBI-1046727
SSR1P433072MINT-4546760
CANXENSP000002474613STRING (score=.962)
DERL2ENSP000001587713STRING (score=.935)
XBP1ENSP000003431553STRING (score=.934)
About this table

2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006986 response to unfolded protein IEA--
GO:0030433 ER-associated protein catabolic process ISS--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for EDEM1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EDEM1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EDEM1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014674

              Sigma-Aldrich siRNA for EDEM1  
                     Sigma-Aldrich shRNA for EDEM1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_014674  

REFSEQ mRNAs for EDEM1 gene: 

NM_014674.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_014674  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014674
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014674
                                 untagged cDNA clone in CMV expression vector: NM_014674 

Additional cDNA sequence: 

AK292643.1 AK298511.1 AK302065.1 BC019088.1 CR597249.1 CR621147.1 D86967.1 

11 DOTS entries:

DT.95370360  DT.120888966  DT.99938746  DT.95365988  DT.100785750  DT.120888884  DT.120888904  DT.120888929 
DT.91878434  DT.92045737  DT.92462892 

24/152 AceView cDNA sequences (see all 152 ):

BM469438 AA885205 AA258630 BU621704 BQ434131 BM542988 N32546 BF511061 
CA431686 CK904356 BX352520 BC019088 BX956626 AA287304 BU618490 BU623214 
BG057331 AA250852 AA369222 CD246059 BE046428 AI142424 CB135001 BG255753 

highest scoring ESTs for EDEM1:

D86967 AA287304 AA885205 AI418151 AI923399 AL692059 AL704145 AW340040 AW499811 AW500254 

Unigene Cluster for EDEM1:

ER degradation enhancer, mannosidase alpha-like 1
Hs.224616  [show with all ESTs]
Unigene Representative Sequence: NM_014674


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000256497  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

EDEM1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for EDEM1

1 / 2 / 3

3 probe-sets matching EDEM1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
31898_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D86967 1.00 1.00 1.00 1

203279_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014674 0.60 1.00 0.82 1

203279_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  EDEM1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AGACATTCCT

SOURCE GeneReport for Unigene cluster: Hs.224616

Expression variation in blood from EXPOLDB for EDEM1

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for EDEM1 gene from 5/12 species (see all 12 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
EDEM11   -- ER degradation enhancer, mannosidase alpha-like 1 88.76(n)
92.64(a)
476547  XM_533753.2  XP_533753.2 
rat
(Rattus norvegicus)
Edem11   -- ER degradation enhancer, mannosidase alpha-like 1 88.75(n)
93.37(a)
297504  XM_238366.4  XP_238366.4 
mouse
(Mus musculus)
Edem11, 5 65
ER degradation enhancer, mannosidase alpha-like 11, 5 88.5(n)1
93.84(a)1
1921931  NM_138677.21  NP_619618.11 
 AA7173285  AB0428285  (see all 29)
chicken
(Gallus gallus)
EDEM11   -- ER degradation enhancer, mannosidase alpha-like 1 78.05(n)
84.07(a)
416110  NM_001006143.1  NP_001006143.1 
zebrafish
(Danio rerio)
zgc558162   -- similar to ER degradation enhancing alpha mannosidase-like 75.86(n) 394164  BC045461.1 
About this table        Species with no ortholog for EDEM1

ENSEMBL Gene Tree for EDEM1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for EDEM1 gene
EDEM22  EDEM32  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/119 NCBI SNPs in EDEM1 are shown (see all 119 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 68)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 3 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs4610771,2
A,C,F,H5235130(-) CCCAGG/TATTTC 1 -- ut31 ese35Minor allele frequency- T:0.07MN EU EA WA 592
rs4577121,2
A,C,F,H5236661(-) TAAAAG/AAGAAA 1 -- ng3110Minor allele frequency- A:0.05NS EA EU WA 1790
rs26486461,2
A,C,F,H5233985(-) AGTCAG/ACCCTG 1 -- ut314Minor allele frequency- A:0.17EU EA WA 412
rs3704761,2
C,F,H,O5233431(-) TACCAT/CCCAAA 1 -- ut318Minor allele frequency- C:0.18EA NA MN EU WA 666
--
rs67658981,2
A,C,F,H5202663(+) GACAGG/AAGCTG 1 -- ng514Minor allele frequency- A:0.40EU EA WA 412
rs76257111,2
A,C,F,H5233236(+) TTGGGG/CTCTTC 1 -- ut314Minor allele frequency- C:0.12EU EA WA 412
rs4643921,2
A,C,F5235299(-) CCACAT/CTGAGC 1 -- ut31 ese31Minor allele frequency- C:0.45MN 184
rs76214281,2
A,C,H5234480(+) GTTAAA/TTACAC 1 -- ut31 ese34Minor allele frequency- T:0.00EU EA WA 382
--
rs3419841,2
A,C,F5233078(-) ctactC/Tgggag 1 -- ut31 ese31Minor allele frequency- T:0.45MN 184
rs4570161,2
A,C5237076(-) CACACA/GCTTCC 1 -- ng310--------
About this table

HapMap Linkage Disequilibrium images for EDEM1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607673

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/29 PubMed articles for EDEM1 gene (see all 29 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 9695 HGNC: 18967 AceView: EDEM1 Ensembl:ENSG00000134109 euGenes: HUgn9695
ECgene: EDEM1 H-InvDB: EDEM1
(According to HUGE)
About This Section

HUGE: KIAA0212
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for EDEM1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for EDEM1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for EDEM1 Antibodies for EDEM1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for EDEM1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
 Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
 Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
 Recombinant & Natural Proteins  

 Recombinant Proteins (EDEM1)
 Antibodies (EDEM1)
 Chimera RNAi (EDEM1)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(EDEM1)
 Antibodies (EDEM1)
 Search OriGene for EDEM1
 Search Tocris compounds for EDEM1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for EDEM1

GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

Random Gene From GIFtS : about GIFtS

Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

Developed at the Crown Human Genome Center & Weizmann Institute of Science



The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
EDEM1 Gene at Home site.
Version 2.41.1
server5.xennexinc.com