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ECT2 Gene

protein-coding   GIFtS: 63
GCID: GC03P172468

Epithelial Cell Transforming 2

(Previous name: epithelial cell transforming sequence 2 oncogene)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Epithelial Cell Transforming 21 2
Epithelial Cell Transforming Sequence 2 Oncogene1 2
Epithelial Cell-Transforming Sequence 2 Oncogene2 3
ARHGEF312
Protein ECT22

External Ids:    HGNC: 31551   Entrez Gene: 18942   Ensembl: ENSG000001143467   OMIM: 6005865   UniProtKB: Q9H8V33   

Export aliases for ECT2 gene to outside databases

Previous GC identifers: GC03P169518 GC03P173499 GC03P173870 GC03P173793 GC03P173773 GC03P173951 GC03P169843


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for ECT2 Gene:
The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related
to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with
the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed
that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is
expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in
the regulation of cytokinesis. Several transcript variants encoding two different isoforms have been found for
this gene. (provided by RefSeq, Apr 2012)

GeneCards Summary for ECT2 Gene:
ECT2 (epithelial cell transforming 2) is a protein-coding gene. Diseases associated with ECT2 include focal segmental glomerulosclerosis, and oral cancer. GO annotations related to this gene include protein homodimerization activity and signal transducer activity. An important paralog of this gene is TOPBP1.

UniProtKB/Swiss-Prot: ECT2_HUMAN, Q9H8V3
Function: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine
nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for
signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex
that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring
formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the
equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in
metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in
the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a
polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite
outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1
through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately
drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast
cancer cells leading to their cell death

Gene Wiki entry for ECT2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000003.12  NT_005612.17  NC_018914.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the ECT2 gene promoter:
         E2F-3a   E2F-4   E2F-5   HOXA3   E2F-2   c-Ets-1   E2F-1   E2F   FOXO1a   FOXO1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): ECT2 promoter sequence
   Search Chromatin IP Primers for ECT2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ECT2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q26.1-q26.2   Ensembl cytogenetic band:  3q26.31   HGNC cytogenetic band: 3q26.1-q26.2

ECT2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ECT2 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P172468:  view genomic region     (about GC identifiers)

Start:
172,468,472 bp from pter      End:
172,539,264 bp from pter
Size:
70,793 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: ECT2_HUMAN, Q9H8V3 (See protein sequence)
Recommended Name: Protein ECT2  
Size: 914 amino acids; 103505 Da
Subunit: Interacts with NR1I3 (By similarity). Homodimer. Homooligomer. Found in the centralspindlin complex.
Interacts (Thr-359 phosphorylated form) with PARD6A; the interaction is observed in cancer cells. Interacts
(Thr-359 phosphorylated form) with PRKCI; the interaction is observed in cancer cells. Interacts with PKP4; the
interaction is observed at the midbody. Interacts with RACGAP1; the interaction is direct, occurs in a
microtubule-dependent manner, is inhibited in metaphase by phosphorylation of ECT2 on Thr-373 and is stimulated
in early anaphase by dephosphorylation of ECT2 probably on Thr-373 through CDK1 activity. Interacts with PLK1;
the interaction is stimulated upon its phosphorylation on Thr-444. Associates with RACGAP1 at anaphase and during
cytokinesis. Interacts with KIF23, PARD3, PARD6A, PARD6B and PRKCQ
Sequence caution: Sequence=BAA91624.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
1 PDB 3D structure from and Proteopedia for ECT2:
3L46 (3D)    
Secondary accessions: Q0MT80 Q2M269 Q6U836 Q9NSV8 Q9NVW9
Alternative splicing: 4 isoforms:  Q9H8V3-1   Q9H8V3-2   Q9H8V3-3   Q9H8V3-4   (May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ECT2: NX_Q9H8V3

Explore proteomics data for ECT2 at MOPED

Post-translational modifications: 

  • Phosphorylated by PLK1 in vitro. Hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at
    Thr-373 occurs during the G2/M phase, relieves its auto-inhibition status and stimulates its GEF activity.
    Phosphorylation at Thr-444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange
    activation. Dephosphorylated at the time of cytokinesis. Phosphorylation at Thr-359 is required for its
    transformation activity in cancer cells1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See ECT2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001245244.1  NP_001245245.1  NP_060568.3  

    ENSEMBL proteins: 
     ENSP00000232458   ENSP00000376457   ENSP00000415876   ENSP00000403501   ENSP00000403446  
     ENSP00000412331   ENSP00000391695   ENSP00000412028   ENSP00000389108   ENSP00000412259  
     ENSP00000411674   ENSP00000397788   ENSP00000443160   ENSP00000401910  
    Reactome Protein details: Q9H8V3

    ECT2 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
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    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for ECT2
    OriGene Custom MassSpec
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    Novus Biologicals ECT2 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for ECT2

    ECT2 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of ECT2
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    Abcam antibodies for ECT2
    Cloud-Clone Corp. Antibodies for ECT2
    ThermoFisher Antibodies for ECT2
    LSBio Antibodies in human, mouse, rat for ECT2

    ECT2 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for ECT2
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for ECT2
    Cloud-Clone Corp. CLIAs for ECT2


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ARHGEF: Rho guanine nucleotide exchange factors

    Selected InterPro protein domains (see all 6):
     IPR001331 GDS_CDC24_CS
     IPR000219 DH-domain
     IPR026817 Ect2
     IPR001849 Pleckstrin_homology
     IPR011993 PH_like_dom

    Graphical View of Domain Structure for InterPro Entry Q9H8V3

    ProtoNet protein and cluster: Q9H8V3

    3 Blocks protein domains:
    IPB000219 DH domain
    IPB001357 BRCT domain
    IPB001849 Pleckstrin-like


    UniProtKB/Swiss-Prot: ECT2_HUMAN, Q9H8V3
    Domain: The BRCT domain 1 and 2 are required for the intramolecular interaction, but not for the intermolecular
    oligomerization. The BRCT domains negatively inhibit its GEF activity in interphase cells. The same BRCT domains
    may act as a positive regulatory motif for the completion of cytokinesis after the breakdown of nuclear membrane
    during mitosis
    Similarity: Contains 2 BRCT domains
    Similarity: Contains 1 DH (DBL-homology) domain
    Similarity: Contains 1 PH domain


    ECT2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ECT2_HUMAN, Q9H8V3
    Function: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine
    nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for
    signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex
    that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring
    formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the
    equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in
    metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in
    the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a
    polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite
    outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1
    through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately
    drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast
    cancer cells leading to their cell death
    Induction: Up-regulated by calcium in cells forming cell-cell contact sites. Up-regulated by DNA damaging agents
    like H(2)O(2) or ionizing radiation (IR)

         Genatlas biochemistry entry for ECT2:
    mouse Ect2 related proto-oncogene 2

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004871signal transducer activity IMP12761501
    GO:0005089Rho guanyl-nucleotide exchange factor activity IDA12761501
    GO:0005096GTPase activator activity IMP15642749
    GO:0005515protein binding IPI15254234
    GO:0017048Rho GTPase binding IEA--
         
    ECT2 for ontologies           About GeneDecksing


    Phenotypes:
         3 GenomeRNAi human phenotypes for ECT2:
     Cell division defect  Increased 8N DNA content  Increased cell death in breast 

         3 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Ect2):
     embryogenesis  mortality/aging  no phenotypic analysis 

    ECT2 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Ect2tm1.2Cjd for ECT2

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ECT2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for ECT2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for ECT2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for ECT2

    miRNA
    Products:
        
    miRTarBase miRNAs that target ECT2:
    hsa-mir-193b-3p (MIRT016363), hsa-mir-26b-5p (MIRT029452), hsa-mir-30c-5p (MIRT047830), hsa-mir-215-5p (MIRT024659), hsa-mir-192-5p (MIRT026504), hsa-mir-96-5p (MIRT027900), hsa-mir-132-3p (MIRT021839), hsa-mir-27a-3p (MIRT028718)

    Block miRNA regulation of human, mouse, rat ECT2 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ECT2 (see all 20):
    hsa-miR-302d hsa-miR-520e hsa-miR-520d-5p hsa-miR-449c* hsa-miR-624* hsa-miR-302e hsa-miR-223 hsa-miR-520a-3p
    SwitchGear 3'UTR luciferase reporter plasmidECT2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for ECT2
    Predesigned siRNA for gene silencing in human, mouse, rat ECT2

    Gene Editing
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    Clone
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    OriGene clones in human, mouse for ECT2 (see all 9)
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    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ECT2 (NM_018098)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ECT2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ECT2

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for ECT2 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ECT2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ECT2_HUMAN, Q9H8V3: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell
    junction. Cell junction, tight junction. Note=Sequestered within the nucleus during interphase. Dispersed
    throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the
    centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase
    and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization
    to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton5
    nucleus5
    cytosol4

    Gene Ontology (GO): Selected cellular component terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005737cytoplasm IDA--
    GO:0005829cytosol TAS--
    GO:0005911cell-cell junction IDA15254234
    GO:0005923tight junction IDA15254234

    ECT2 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ECT2 About   (see all 10)  
    See pathways by source

    SuperPathContained pathways About
    1p75 NTR receptor-mediated signalling
    p75 NTR receptor-mediated signalling0.74
    NRAGE signals death through JNK0.71
    Cell death signalling via NRAGE, NRIF and NADE0.74
    G alpha (12/13) signalling events0.44
    2Signaling by GPCR
    Signaling by GPCR0.90
    Signal Transduction0.58
    GPCR downstream signaling0.90
    3Rho GTPase cycle
    Rho GTPase cycle1.00
    Signaling by Rho GTPases1.00
    4Signaling by FGFR
    Signalling by NGF0.71
    5Regulation of RhoA activity
    Regulation of RhoA activity

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    3 GeneGo (Thomson Reuters) Pathways for ECT2
        G-protein signaling Regulation of RAC1 activity
    G-protein signaling Regulation of CDC42 activity
    G-protein signaling RhoA regulation pathway

    3 BioSystems Pathways for ECT2
        Gastric cancer network 1
    PLK1 signaling events
    Regulation of RhoA activity

    3 Reactome Pathways for ECT2
        G alpha (12/13) signalling events
    NRAGE signals death through JNK
    Rho GTPase cycle



    ECT2 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Array including ECT2: 
              Prostate Cancer in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for ECT2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for ECT2 (Q9H8V31, 2, 3 ENSP000002324584) via UniProtKB, MINT, STRING, and/or I2D (see all 636)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RACGAP1Q9H0H52, 3, ENSP000003098714MINT-6166634 MINT-7947479 I2D: score=2 STRING: ENSP00000309871
    NCK1P163331, 3, ENSP000002889864EBI-1054039,EBI-389883 I2D: score=2 STRING: ENSP00000288986
    PLK1P533502, 3, ENSP000003000934MINT-7947479 I2D: score=1 STRING: ENSP00000300093
    PIK3R1P279862, 3, ENSP000002743354MINT-8111919 I2D: score=3 STRING: ENSP00000274335
    GRB2P629933, ENSP000003390074I2D: score=2 STRING: ENSP00000339007
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 26):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000902cell morphogenesis IEA--
    GO:0000910cytokinesis IMP16236794
    GO:0007264small GTPase mediated signal transduction TAS--
    GO:0015031protein transport IEA--
    GO:0032147activation of protein kinase activity IDA15254234

    ECT2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ECT2



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for ECT2 gene (3 alternative transcripts): 
    NM_001258315.1  NM_001258316.1  NM_018098.5  

    Unigene Cluster for ECT2:

    Epithelial cell transforming sequence 2 oncogene
    Hs.518299  [show with all ESTs]
    Unigene Representative Sequence: NM_001258316
    17 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000232458(uc003fii.2 uc003fij.1 uc003fik.1) ENST00000392692(uc010hwv.1 uc003fil.1)
    ENST00000417960(uc003fih.2) ENST00000428567 ENST00000366090 ENST00000426894
    ENST00000366254 ENST00000415665 ENST00000438041 ENST00000441497 ENST00000444250
    ENST00000437296(uc003fim.1) ENST00000460860 ENST00000486027 ENST00000487073
    ENST00000540509 ENST00000427830
    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate ECT2 (see all 20):
    hsa-miR-302d hsa-miR-520e hsa-miR-520d-5p hsa-miR-449c* hsa-miR-624* hsa-miR-302e hsa-miR-223 hsa-miR-520a-3p
    SwitchGear 3'UTR luciferase reporter plasmidECT2 3' UTR sequence
    Inhib. RNA
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    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: ECT2 (NM_018098)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ECT2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ECT2
    Primer
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      QuantiTect SYBR Green Assays in human, mouse, rat ECT2
      QuantiFast Probe-based Assays in human, mouse, rat ECT2

    Additional mRNA sequence: 

    AK001323.1 AK001515.1 AK023267.1 AK027713.1 AK308391.1 AK314581.1 AL137710.1 AY376439.1 
    BC006838.1 BC006987.1 BC070038.1 BC112086.1 DQ847274.1 

    17 DOTS entries:

    DT.97792898  DT.91760878  DT.95197878  DT.120841247  DT.95197867  DT.120841246  DT.120841265  DT.95197895 
    DT.100790050  DT.95197876  DT.120841248  DT.95197881  DT.95197893  DT.97770594  DT.99929308  DT.99990338 
    DT.97789650 

    Selected AceView cDNA sequences (see all 181):

    AW511311 CB143701 AI738508 AK027713 BM995738 BG613806 AU133302 AA992180 
    CD652117 CB120629 BU161769 AL137710 BQ925030 AK001515 BQ229951 AA343765 
    AU128730 BM827335 AI038579 AA279942 AW504433 CB128759 AI589007 AI354483 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for ECT2 (see all 13)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11a · 11b ^ 12a · 12b ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17 ^ 18 ^
    SP1:                                            -                                                                                                               
    SP2:                                                                                                                                                            
    SP3:                    -     -                 -                                                                                                               
    SP4:                                                                                                                                                            
    SP5:                    -     -                                                                                                                                 

    ExUns: 19 ^ 20 ^ 21 ^ 22a · 22b ^ 23a · 23b ^ 24a · 24b ^ 25a · 25b ^ 26 ^ 27a · 27b ^ 28a · 28b
    SP1:                                      -     -                                                   
    SP2:                                      -     -                             -     -               
    SP3:                                                                                                
    SP4:                                      -     -                             -                     
    SP5:                                                                                                


    ECgene alternative splicing isoforms for ECT2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ECT2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TATGTGCCAC
    ECT2 Expression
    About this image


    ECT2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 5) fully expand
     
     Neural Tube (Nervous System)    fully expand to see all 3 entries
             Mesencephalic Ventricular Zone
     
     Heart (Cardiovascular System)
             Cardiac Fibroblasts Myocardium
     
     Fibroblasts
             Cardiac Fibroblasts Myocardium
     
     Gonad (Reproductive System)
             Primordial Germ Cells Primitive Gonad
     
     Brain (Nervous System)
             Hypothalamus
    ECT2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ECT2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.518299

    UniProtKB/Swiss-Prot: ECT2_HUMAN, Q9H8V3
    Tissue specificity: Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma,
    primary non-small cell lung cancer tumors and lung adenocarcinoma

        Pathway & Disease-focused RT2 Profiler PCR Array including ECT2: 
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for ECT2 gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ect21 , 5 ect2 oncogene1, 5 87.15(n)1
    91.35(a)1
      3 (10.82 cM)5
    136051  NM_007900.31  NP_031926.21 
     270972225 
    chicken
    (Gallus gallus)
    Aves ECT21 epithelial cell transforming sequence 2 oncogene 78.93(n)
    83.13(a)
      424983  XM_004943470.1  XP_004943527.1 
    lizard
    (Anolis carolinensis)
    Reptilia ECT26
    epithelial cell transforming sequence 2 oncogene
    73(a)
    1 ↔ 1
    3(6098697-6147447)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia ect21 epithelial cell transforming sequence 2 oncogene 74.46(n)
    77.99(a)
      100127840  NM_001113146.1  NP_001106617.1 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.126792 Transcribed sequence with weak similarity to protein more 71.29(n)    BI876682.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta pbl1 pebble 47.41(n)
    39.45(a)
      38879  NM_079241.3  NP_523965.2 
    worm
    (Caenorhabditis elegans)
    Secernentea ect-26
    Protein ECT-2, isoform b (ect-2) mRNA, complete cd...
    18(a)
    1 ↔ 1
    II(10778395-10783289) WBGene00002297


    ENSEMBL Gene Tree for ECT2 (if available)
    TreeFam Gene Tree for ECT2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ECT2 gene
    TOPBP12  
    1 SIMAP similar gene for ECT2 using alignment to 10 protein entries:     ECT2_HUMAN (see all proteins):
    TOPBP1

    ECT2 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for ECT2
    PGOHUM00000245309


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ECT2 (see all 1446)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0359754
    A breast cancer sample4--see VAR_0359752 T P mis40--------
    rs1877600451,2
    --172466480(+) TTGCCG/TTTGCA 3 -- us2k10--------
    rs738802561,2
    C,F--172466541(+) CCAGAC/TACCAG 3 -- us2k14Minor allele frequency- T:0.24WA CSA 124
    rs1387576961,2
    --172466748(+) CCTACA/TGTTTC 3 -- us2k10--------
    rs1911312851,2
    --172467041(+) TACCCA/GTTTAT 3 -- us2k10--------
    rs800351591,2
    C,F--172467113(+) CTTAAA/TATTCA 3 -- us2k12Minor allele frequency- T:0.03NA 122
    rs789287271,2
    --172467177(+) GAAAGA/CGGTGC 3 -- us2k10--------
    rs1837676821,2
    --172467456(+) GATTGA/CCTGAC 3 -- us2k10--------
    rs1473043121,2
    --172467618(+) GAACTA/GTGGAA 3 -- us2k10--------
    rs130705601,2
    C,F--172467699(+) AATTAC/TTTTCA 3 -- us2k1 tfbs38Minor allele frequency- T:0.41MN EA NA WA 1616

    HapMap Linkage Disequilibrium report for ECT2 (172468472 - 172539264 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for ECT2:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv877991CNV Loss21882294
    nsv877990CNV Loss21882294
    nsv7356OTHER Inversion18451855

    Site Specific Mutation Identification with PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 600586    OMIM disorders: --

    20 diseases for ECT2:    
    About MalaCards
    focal segmental glomerulosclerosis    oral cancer    lung adenocarcinoma    esophageal cancer
    astrocytoma    breast and colorectal cancer    esophagitis    pancreatic cancer
    adenocarcinoma    pancreatitis    squamous cell carcinoma    obesity
    lung cancer    prostate cancer    prostatitis    endotheliitis
    colorectal cancer    malaria    breast cancer    neuronitis


    ECT2 for disorders           About GeneDecksing

    Genetic Association Database (GAD): ECT2
    Human Genome Epidemiology (HuGE) Navigator: ECT2 (1 document)

    Export disorders for ECT2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ECT2 gene, integrated from 10 sources (see all 79):
    (articles sorted by number of sources associating them with ECT2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis. (PubMed id 10579713)1, 2, 3 Tatsumoto T.... Miki T. (J. Cell Biol. 1999)
    2. The tandem BRCT domains of Ect2 are required for both negative and positive regulation of Ect2 in cytokinesis. (PubMed id 15545273)1, 2, 9 Kim J.E.... Chen J. (J. Biol. Chem. 2005)
    3. Rho exchange factor ECT2 is induced by growth factors and regulates cytokinesis through the N-terminal cell cycle regulator-related domains. (PubMed id 14587037)1, 2, 9 Saito S.... Miki T. (J. Cell. Biochem. 2003)
    4. Phosphorylation of the cytokinesis regulator ECT2 at G2/M phase stimulates association of the mitotic kinase Plk1 and accumulation of GTP-bound RhoA. (PubMed id 16247472)1, 2, 9 Niiya F.... Miki T. (Oncogene 2006)
    5. Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation. (PubMed id 19468300)1, 2, 9 Wolfe B.A.... Glotzer M. (PLoS Biol. 2009)
    6. The armadillo protein p0071 regulates Rho signalling during cytokinesis. (PubMed id 17115030)1, 2, 9 Wolf A.... Hatzfeld M. (Nat. Cell Biol. 2006)
    7. Oncogenic activity of Ect2 is regulated through protein kinase C iota-mediated phosphorylation. (PubMed id 21189248)1, 2 Justilien V.... Fields A.P. (J. Biol. Chem. 2011)
    8. The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage. (PubMed id 21373644)1, 2 Srougi M.C. and Burridge K. (PLoS ONE 2011)
    9. A large-scale candidate gene association study of age at menarche and age at natural menopause. (PubMed id 20734064)1, 4 He C....Hunter D.J. (Hum. Genet. 2010)
    10. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. (PubMed id 19468302)1, 2 Burkard M.E.... Jallepalli P.V. (PLoS Biol. 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 1894 HGNC: 3155 AceView: ECT2 Ensembl:ENSG00000114346 euGenes: HUgn1894
    ECgene: ECT2 H-InvDB: ECT2

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for ECT2 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for ECT2 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ECT2 gene:
    Search GeneIP for patents involving ECT2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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