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ECOP Gene

protein-coding GIFtS: 40

GC07M055506

EGFR-coamplified and overexpressed protein
symbol from the EntrezGene Database
NOT approved by the HUGO Gene Nomenclature Committee (HGNC).

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
DKFZp564K0822 ^²
ECop ^³
FLJ20532 ^²
GASP ^², ³, ⁵

Descriptions
EGFR-coamplified and overexpressed protein ^²
Glioblastoma-amplified secreted protein ^³
Putative NF-kappa-B-activating protein 055N ^³

External Ids
Entrez Gene: 81552²
UniProtKB: Q96AW1³
Ensembl: ENSG00000154978⁷

Search outside databases for aliases for ECOP gene

Previous GC identifer: GC07M055313

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: ECOP_HUMAN, Q96AW1

Function: Increases the transcriptional activity of NFKB1 by facilitating its nuclear

translocation, DNA-binding and associated apoptotic response, when overexpressed

Gene Wiki entry for ECOP

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the ECOP gene

Entrez Gene cytogenetic band: 7p11.2 Ensembl cytogenetic band: 7p11.2

ECOP Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7 GeneLoc Exon Structure

GeneLoc location for GC07M055506: (about GC identifiers)

Start:	55,505,800 bp from pter
End:	55,607,694 bp from pter
Size:	101,895 bases
Orientation:	minus strand

1 alternative location:

Chr7-,CRA_TCAG 55,542,585-55,644,447

RefSeq DNA sequence:

NC_000007.12 NT_033968.5 NT_079592.2

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: ECOP_HUMAN, Q96AW1 (See protein sequence)

Recommended Name: EGFR-coamplified and overexpressed protein precursor

Size: 172 amino acids; 19224 Da

Subcellular location: Nucleus. Membrane; Single-pass type I membrane protein (Potential). Note=When

overexpressed, localizes in the nucleus and perinuclear regions

Sequence caution: Sequence=AAK82888.1; Type=Erroneous translation; Note=Wrong choice of frame;

Secondary accessions: B2RAT4 Q6FIE3 Q8NBN8 Q96RE5 Q9H0W4

REFSEQ proteins: NP_110423.3

ENSEMBL proteins:

ENSP00000285279

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for ECOP:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for ECOP

Assays for ECOP:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ProtoNet protein and cluster: Q96AW1

UniProtKB/Swiss-Prot: ECOP_HUMAN, Q96AW1

Similarity: Belongs to the ECOP family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (ECOP)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_030796

Applied Biosystems Silencer® siRNAs for ECOP

Sigma-Aldrich siRNA for ECOP

Sigma-Aldrich shRNA for ECOP

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_030796
Myc/DDK tagged cDNA clone in CMV expression vector: NM_030796
untagged cDNA clone in CMV expression vector: NM_030796

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_030796

UniProtKB/Swiss-Prot: ECOP_HUMAN, Q96AW1

Function: Increases the transcriptional activity of NFKB1 by facilitating its nuclear

translocation, DNA-binding and associated apoptotic response, when overexpressed

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for ECOP

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (ECOP)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_030796

Sigma-Aldrich siRNA for ECOP

Sigma-Aldrich shRNA for ECOP

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_030796

REFSEQ mRNAs for ECOP gene:

NM_030796.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_030796

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_030796
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_030796
                                 untagged cDNA clone in CMV expression vector: NM_030796

Additional cDNA sequence:

AB097004.1 AF395824.1 AK000539.1 AK075391.1 AK090825.1 AK092992.1 AK126848.1 AK225755.1

AK309288.1 AK314338.1 AK315882.1 AL136610.1 BC016650.1 CR533483.1 CR606724.1

24 DOTS entries:

DT.97772732 DT.455806 DT.100731418 DT.91859296 DT.100031062 DT.100815455 DT.100815453 DT.121100173

DT.99964077 DT.100815458 DT.121100241 DT.100815457 DT.102844864 DT.121100138 DT.121100140 DT.101967680

DT.121100152 DT.121100224 DT.121100226 DT.121100237 DT.121460203 DT.121460411 DT.95123860 DT.121460604

24/378 AceView cDNA sequences (see all 378 ):

BQ638915 AK000539 CD107342 BM693055 CD518325 CA437966 BU507685 CR606724

BM666268 CD108757 BM555009 AA862278 T30346 BQ001936 BG110964 AI097627

BQ232964 BU075594 CD366489 AA071418 BQ421973 BX956224 AX746636 CA748494

highest scoring ESTs for ECOP:

AB097004 AK075391 AL136610 AL558370 AU280098 BC016650 BF795397 BG394478 BG701057 BI547098

Unigene Cluster for ECOP:

EGFR-coamplified and overexpressed protein

Hs.488307 [show with all ESTs]

Unigene Representative Sequence: AK126848

GeneLoc Exon Structure

5/14 Alternative Splicing Database (ASD) splice patterns (SP) for ECOP (see all 14 )

ExUns:	1	^	2a	·	2b	·	2c	^	3a	·	3b	·	3c	^	4a	·	4b	^	5	^	6	^	7	^	8	^	9	^	10	^	11	^	12a	·	12b	^	13	^	14a	·	14b	^	15a	·	15b	·	15c	^	16	^	17a	·
SP1:																	-		-		-		-		-		-				-						-		-		-
SP2:																							-		-		-				-						-		-		-
SP3:			-		-		-		-		-		-		-		-		-		-		-		-		-				-
SP4:									-		-		-		-		-		-		-		-		-		-				-						-		-		-
SP5:									-		-		-						-		-		-		-		-				-						-		-		-

ExUns:	17b	·	17c	·	17d	·	17e	·	17f	·	17g	·	17h	·	17i
SP1:
SP2:
SP3:
SP4:
SP5:

About this scheme

1 Ensembl transcript including schematic representation:

ENST00000285279

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

ECOP expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for ECOP

¹ / ² / ³

3 probe-sets matching ECOP gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
34830_at²^{, 3}	U95-A	2	1.00	0.53	1.00	1.00	W25986	0.60	0.58	0.59	1
208091_s_at²^{, 3}	U133-A	2	1.00	0.55	--	--	NM_030796	0.60	1.00	0.82	1
208091_s_at²	U133Plus2	2	1.00	0.55	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: CTTTTCCTCT

SOURCE GeneReport for Unigene cluster: Hs.488307

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for ECOP gene from 5/8 species (see all 8 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	LOC608562¹	--	similar to EGFR-coamplified and overexpressed protein	89.46(n) 93.55(a)	608562 XM_845618.1 XP_850711.1
chimpanzee (Pan troglodytes)	LOC741927¹	--	EGFR-coamplified and overexpressed protein	99.81(n) 100(a)	741927 XM_001157372.1 XP_001157372.1
cow (Bos taurus)	ECOP¹	--	EGFR-coamplified and overexpressed protein	87.15(n) 88.55(a)	767861 NM_001076972.1 NP_001070440.1
rat (Rattus norvegicus)	Ecop¹	--	EGFR-coamplified and overexpressed protein	83.91(n) 89.53(a)	362374 XM_342698.3 XP_342699.3
mouse (Mus musculus)	AW146242^{1, 5}	6⁵	expressed sequence AW146242^{1, 5}	84.11(n)¹ 89.53(a)¹	232023¹ NM_146168.1¹ NP_666280.1¹ AA474160⁵ AI429552⁵ (see all 15)

About this table Species with no ortholog for ECOP

ENSEMBL Gene Tree for ECOP

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/492 NCBI SNPs in ECOP are shown (see all 492 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 112)

AB	SNP ID	Valid	Chr 7 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs17172486^1,2	F,H	55505587(+)	`TCAGCG/AATGCT`	1	--	ng5¹		7		NA EU EA WA	560
--	rs1722985^1,2	C,F	55505397(-)	`attttA/Gtgttt`	1	--	ng5¹		4		EA NA	146
	rs815957^1,2	A,C,F,H	55508201(+)	`GGGTCA/GGTGTA`	1	T/T	syn¹ ese³		9		MN EA NA EU WA	806
--	rs11238366^1,2	A,C	55508213(+)	`TAGGGC/TGGCCC`	1	P/P	syn¹ ese³		0	--	--	--	--
	rs1723898^1,2	A,C,F,H,O	55537799(-)	`TTAGGT/GCCCCA`	1	--	int¹		8		EA NA MN EU WA	648
	rs1880528^1,2	A,C,F,H,O	55589218(-)	`TAAAGT/AGGTCT`	1	--	int¹		15		NA EU EA WA	1392
	rs2222589^1,2	A,C,F,H,O	55588742(-)	`GTATCA/GTTGGT`	1	--	int¹		7		EA NA EU	504
	rs6957545^1,2	A,C,F,H	55518187(+)	`AAGCAT/CCTGCC`	1	--	int¹		4		EU EA WA	366
	rs1404564^1,2	C,F,H,O	55594444(-)	`GCCAAG/TAGTGG`	1	--	int¹		14		NA EA EU WA	1218
	rs815951^1,2	C,F,H,O	55527701(+)	`GGGTCC/TGGGAC`	1	--	int¹		10		EU EA WA NA	598

About this table

HapMap Linkage Disequilibrium images for ECOP (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 611915

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/16 PubMed articles for ECOP gene (see all 16 ):

ECop (EGFR-coamplified and overexpressed protein), a novel protein, regulates NF-kappaB transcriptional activity and associated apoptotic response in an IkappaBalpha-dependent manner. (PubMed id 15735698)^{1, 3, 4} Park S. and James C.D. (2005)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways. (PubMed id 12761501)^{3, 4} Matsuda A....Sugano S. (2003)
Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)^{3, 4} Wiemann S.... Poustka A. (2001)
Molecular markers in patients with chronic wounds to guide surgical debridement. (PubMed id 17515955)³ Brem H....Tomic-Canic M. (2007)
The LIFEdb database in 2006. (PubMed id 16381901)³ Mehrle A....Wiemann S. (2006)
Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. (PubMed id 16303743)³ Otsuki T....Isogai T. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)⁴ Gerhard D.S....Malek J. (2004)
From ORFeome to biology: a functional genomics pipeline. (PubMed id 15489336)³ Wiemann S....Poustka A. (2004)
Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. (PubMed id 15146197)³ Brandenberger R.... Stanton L.W. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 81552

AceView: DKFZP564K0822

Ensembl:ENSG00000154978

euGenes: HUgn81552

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for ECOP
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section

Products for ECOP:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for ECOP-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for ECOP		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for ECOP		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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Search Tocris compounds for ECOP

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for ECOP

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ECOP Gene at Home site.
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ECOP Gene

protein-coding GIFtS: 40

Aliases &
Descriptions
for ECOP

Summaries
for ECOP

Genomic Location
for ECOP

Proteins
for ECOP

Protein Domains/
Families
for ECOP

Gene Function
for ECOP

Pathways & Interactions
for ECOP

Drugs & Compounds
for ECOP

Transcripts
for ECOP

Expression
for ECOP

Orthologs
for ECOP

Paralogs
for ECOP

SNPs/Variants
for ECOP

Disorders & Mutations
for ECOP

Medical News
for ECOP

Publications
for ECOP

Search for ECOP

Genome Databases showing ECOP

Other Databases showing ECOP

Specialized Databases showing ECOP

Licensable Technologies
for ECOP

Services
for ECOP

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

ECOP Gene

protein-coding GIFtS: 40

Aliases & Descriptionsfor ECOP

Summariesfor ECOP

Genomic Locationfor ECOP

Proteinsfor ECOP

Protein Domains/Familiesfor ECOP

Gene Functionfor ECOP

Pathways & Interactionsfor ECOP

Drugs & Compoundsfor ECOP

Transcriptsfor ECOP

Expression for ECOP

Orthologsfor ECOP

Paralogsfor ECOP

SNPs/Variantsfor ECOP

Disorders & Mutationsfor ECOP

Medical Newsfor ECOP

Publicationsfor ECOP

Search for ECOP

Genome Databases showing ECOP

Other Databases showing ECOP

Specialized Databases showing ECOP

Licensable Technologiesfor ECOP

Servicesfor ECOP

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for ECOP

Summaries
for ECOP

Genomic Location
for ECOP

Proteins
for ECOP

Protein Domains/
Families
for ECOP

Gene Function
for ECOP

Pathways & Interactions
for ECOP

Drugs & Compounds
for ECOP

Transcripts
for ECOP

Expression
for ECOP

Orthologs
for ECOP

Paralogs
for ECOP

SNPs/Variants
for ECOP

Disorders & Mutations
for ECOP

Medical News
for ECOP

Publications
for ECOP

Licensable Technologies
for ECOP

Services
for ECOP