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ECM2 Gene

protein-coding   GIFtS: 51

GC09M094297
extracellular matrix protein 2, female organ and adipocyte specific
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
MGC126355 2
MGC126356 2
OTTHUMP00000021654 2
OTTHUMP00000063687 2
Descriptions
Matrix glycoprotein SC1/ECM2 2, 3
extracellular matrix protein 2 2
extracellular matrix protein 2, female organ and adipocyte
specific 2
External Ids
HGNC: 31541
Entrez Gene: 18422
UniProtKB: O947693
Ensembl: ENSG000001068237
Search outside databases for aliases for ECM2 gene

Previous GC identifers: GC09M086084 GC09M086985 GC09M088556 GC09M090599 GC09M090597 GC09M092335

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ECM2:
ECM2 encodes extracellular matrix protein 2, so named because it shares extensive similarity with
known extracelluar matrix protiens. [provided by RefSeq]

UniProtKB/Swiss-Prot: ECM2_HUMAN, O94769
Function: Promotes matrix assembly and cell adhesiveness (By similarity)

Gene Wiki entry for ECM2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ECM2 gene  

Entrez Gene cytogenetic band: 9q22.3   Ensembl cytogenetic band:  9q22.31   HGNC cytogenetic band: 9q22.3

ECM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09M094297:     (about GC identifiers)

Start:
94,295,657 bp from pter
End:
94,338,158 bp from pter
Size:
42,502 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000009.10  NT_008470.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: ECM2_HUMAN, O94769 (See protein sequence)
Recommended Name: Extracellular matrix protein 2 precursor  
Size: 699 amino acids; 79789 Da
Subunit: Interacts with numerous extracellular matrix proteins (By similarity)
Subcellular location: Secreted, extracellular space, extracellular matrix
Secondary accessions: B2R730 Q5T9F2

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_001384.1  

    ENSEMBL proteins: 
    ENSP00000344758 ENSP00000378905 ENSP00000364690 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005578 proteinaceous extracellular matrix TAS9790758
    About this table

    Antibodies for ECM2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for ECM2

    Assays for ECM2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR001611 Leu-rich_rpt
     IPR001007 VWF_C


       GeneDecks  ECM2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O94769

    ProtoNet protein and cluster: O94769

    1 Blocks protein family: IPB001007 von Willebrand factor

    UniProtKB/Swiss-Prot: ECM2_HUMAN, O94769
    Similarity: Belongs to the small leucine-rich proteoglycan (SLRP) family. Class I subfamily
    Similarity: Contains 14 LRR (leucine-rich) repeats
    Similarity: Contains 1 VWFC domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ECM2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001393

                  Applied Biosystems Silencer® siRNAs for ECM2

                  Sigma-Aldrich siRNA for ECM2  
                         Sigma-Aldrich shRNA Panels and shRNA for ECM2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001393
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001393
                                     untagged cDNA clone in CMV expression vector: NM_001393 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001393

    UniProtKB/Swiss-Prot: ECM2_HUMAN, O94769
    Function: Promotes matrix assembly and cell adhesiveness (By similarity)

    Genatlas biochemistry entry for ECM2:
    extracellular matrix 2,predominantly expressed in adipose tissue,mammary gland,ovary and uterus

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005178 integrin binding TAS9790758
    GO:0005515 protein binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    4 Interacting proteins for ECM2 (ENSP000003447583) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EFEMP2ENSP000003099533STRING (score=.843)
    EFEMP1ENSP000003475963STRING (score=.805)
    FBLN5ENSP000002676203STRING (score=.775)
    PSMC3IPENSP000002537893STRING (score=.712)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007160 cell-matrix adhesion TAS9790758
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ECM2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ECM2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_001393

                  Sigma-Aldrich siRNA for ECM2  
                         Sigma-Aldrich shRNA Panels and shRNA for ECM2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001393  

    REFSEQ mRNAs for ECM2 gene: 

    NM_001393.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001393  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_001393
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_001393
                                     untagged cDNA clone in CMV expression vector: NM_001393 

    Additional cDNA sequence: 

    AB011792.1 AK296458.1 AK298278.1 AK303922.1 AK308485.1 AK312820.1 BC036806.2 BC105958.1 
    BC107493.1 BX537976.1 

    10 DOTS entries:

    DT.307831  DT.40219665  DT.101981464  DT.121154242  DT.40128605  DT.97837799  DT.100642041  DT.100678234 
    DT.100742270  DT.40269779 

    24/61 AceView cDNA sequences (see all 61 ):

    CA436989 AW058335 AI090981 AI814725 C16120 CB162125 CA420271 CB162823 
    AA677468 BX537976 BF431290 BX642447 N45178 AW779895 AL711993 BX509899 
    AI123569 AI057125 AB011792 CB962137 CD000459 AI923371 CA414686 BQ181757 

    highest scoring ESTs for ECM2:

    AB011792 AI057125 AI061071 AI761978 AI767691 AI829266 AI923371 AL705981 AL711993 AW058335 

    Unigene Cluster for ECM2:

    Extracellular matrix protein 2, female organ and adipocyte specific
    Hs.117060  [show with all ESTs]
    Unigene Representative Sequence: AB011792


    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for ECM2 (see all 7 )

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b · 10c ^ 11a · 11b · 11c · 11d ^ 12
    SP1:                                      -     -                 -     -                                       
    SP2:                                      -     -           -                                                   
    SP3:                                                        -     -     -                                       
    SP4:                                                                                                            
    SP5:              -                                                                                             

    About this scheme

    ECgene alternative splicing isoforms for ECM2

    3 Ensembl transcripts including schematic representations:
    ENST00000344604  ENST00000395534  ENST00000375540  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ECM2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ECM2

    1 / 2 / 3

    6 probe-sets matching ECM2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    39673_i_at2, 3 U95-A 1 1.00 1.00 0.99 1.09 AB011792 1.00 1.00 1.00 1
    78443_at2, 3 U95-D 1 1.00 1.00 0.86 1.12 AI057125 0.60 1.00 0.82 1

    39674_r_at2, 3 U95-A 1 1.00 1.00 0.96 0.80 AB011792 1.00 1.00 1.00 1

    206101_at2, 3 U133-A 1 1.00 1.00 -- -- NM_001393 0.60 1.00 0.82 1

    206101_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1568779_a_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  ECM2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGTAAAATTA

    SOURCE GeneReport for Unigene cluster: Hs.117060

    Expression variation in blood from EXPOLDB for ECM2

    UniProtKB/Swiss-Prot: ECM2_HUMAN, O94769
    Tissue specificity: Expressed predominantly in adipose tissue as well as female-specific organs
    such as mammary gland, ovary, and uterus

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ECM2 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    ECM21   -- extracellular matrix protein 2, female organ and adipocyte more 83.01(n)
    79.31(a)
    476361  XM_533562.2  XP_533562.2 
    chimpanzee
    (Pan troglodytes)
    ECM21   -- extracellular matrix protein 2, female organ and adipocyte more 99.19(n)
    99.19(a)
    744012  XM_001148362.1  XP_001148362.1 
    cow
    (Bos taurus)
    ECM21   -- extracellular matrix protein 2, female organ and adipocyte more 85.33(n)
    80.59(a)
    533916  NM_001034597.1  NP_001029769.1 
    mouse
    (Mus musculus)
    Ecm21, 5 135
    extracellular matrix protein 2, female organ and adipocyte more1, 5 80.9(n)1
    76.24(a)1
    4078001  NM_001012324.21  NP_001012324.11 
     AK0185615  AK0388125  (see all 12)
    chicken
    (Gallus gallus)
    ECM21   -- extracellular matrix protein 2, female organ and adipocyte more 67.44(n)
    62.48(a)
    415953  XM_414297.2  XP_414297.1 
    About this table        Species with no ortholog for ECM2

    ENSEMBL Gene Tree for ECM2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for ECM2 gene
    BGN2  DCN2  ASPN2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section

      --
    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603479

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/13 PubMed articles for ECM2 gene (see all 13 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 1842 HGNC: 3154 AceView: ECM2 Ensembl:ENSG00000106823 euGenes: HUgn1842
    ECgene: ECM2 H-InvDB: ECM2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ECM2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for ECM2 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ECM2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Search Novus for antibodies for ECM2

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