V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

ECD Gene

protein-coding   GIFtS: 54

GC10M074563
ecdysoneless homolog (Drosophila)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
GCR2 1, 2
HSGT1 2
OTTHUMP00000019795 2
SGT1 3
hSGT1 1, 3
Descriptions
Protein ecdysoneless homolog 3
Suppressor of GCR2 3
ecdysoneless homolog (Drosophila) 2
suppressor of S. cerevisiae gcr2 2
External Ids
HGNC: 170291
Entrez Gene: 113192
UniProtKB: O959053
Ensembl: ENSG000001228827
Search outside databases for aliases for ECD gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: SGT1_HUMAN, O95905
Function: Novel regulator of p53 stability and function. May also be a transcriptional activator
required for the expression of glycolytic genes

Gene Wiki entry for ECD

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ECD gene  

Entrez Gene cytogenetic band: 10q22.1   Ensembl cytogenetic band:  10q22.1   HGNC cytogenetic band: 10q22.3

ECD Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10M074563:     (about GC identifiers)

Start:
74,564,289 bp from pter
End:
74,597,823 bp from pter
Size:
33,535 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000010.9  NT_008583.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SGT1_HUMAN, O95905 (See protein sequence)
Recommended Name: Protein SGT1  
Size: 644 amino acids; 72758 Da
Subunit: Interacts with TP53 and MDM2
Subcellular location: Cytoplasm. Nucleus

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (3 alternative transcripts): 
    NP_001129224.1  NP_001129225.1  NP_009196.1  


    ENSEMBL proteins: 
    ENSP00000362070 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (SGT1)
    Human Recombinant Proteins from Abnova (HSGT1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005737 cytoplasm IEA--
    About this table

    Antibodies for ECD: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for ECD
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (SGT1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (HSGT1)
    Novus Biologicals Antibodies for ECD

    Assays for ECD: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR010770 SGT1


       GeneDecks  ECD for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O95905

    ProtoNet protein and cluster: O95905

    1 Blocks protein family: IPB010770 SGT1

    UniProtKB/Swiss-Prot: SGT1_HUMAN, O95905
    Similarity: Belongs to the SGT1 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ECD)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (HSGT1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_001135752

                  Applied Biosystems Silencer® siRNAs for ECD

                  Sigma-Aldrich siRNA for ECD  
                         Sigma-Aldrich shRNA for ECD  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001135752
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001135752
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_001135752 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001135752

    UniProtKB/Swiss-Prot: SGT1_HUMAN, O95905
    Function: Novel regulator of p53 stability and function. May also be a transcriptional activator
    required for the expression of glycolytic genes

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003713 transcription coactivator activity TAS9928932
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    3 Interacting proteins for ECD (ENSP000003620703) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    NR3C1ENSP000002315093STRING (score=.766)
    GMCL1LENSP000002825703STRING (score=.716)
    HSP90AA2ENSP000003351533STRING (score=.716)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006110 regulation of glycolysis TAS9928932
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0006366 transcription from RNA polymerase II promoter TAS9928932
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ECD

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ECD)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (HSGT1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_001135752

                  Sigma-Aldrich siRNA for ECD  
                         Sigma-Aldrich shRNA for ECD  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001135752  NM_001135753  NM_007265  

    REFSEQ mRNAs for ECD gene (3 alternative transcripts): 

    NM_001135752.1   NM_001135753.1   NM_007265.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001135752  NM_001135753  NM_007265  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001135752
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001135752
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_001135752 

    Additional cDNA sequence: 

    AK022616.1 AK225519.1 AK315711.1 BC000721.2 BC103720.1 CR457407.1 D88208.1 

    16 DOTS entries:

    DT.91916541  DT.101980786  DT.100782194  DT.443126  DT.100694870  DT.102830443  DT.91758695  DT.91758697 
    DT.91938516  DT.100657820  DT.100782191  DT.100782195  DT.91758694  DT.91758703  DT.100782196  DT.86855228 

    24/232 AceView cDNA sequences (see all 232 ):

    N70677 D88208 BG232035 AI890809 CB215450 AA457335 BU753567 AU124922 
    CF529380 AA894689 AA312695 BP356929 BM129820 BP359476 BM458876 BM129619 
    AI289462 AA767475 BP336714 BQ027657 BM681393 BM727744 CA415164 BC000721 

    highest scoring ESTs for ECD:

    D88208 AA306425 AA306721 AA312695 AK022616 AL520988 AU124922 AW955141 BC000721 BE279473 

    Unigene Cluster for ECD:

    Ecdysoneless homolog (Drosophila)
    Hs.631822  [show with all ESTs]
    Unigene Representative Sequence: NM_001135752


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for ECD

    ExUns: 1 ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14 ^ 15
    SP1:                                                                                                                        
    SP2:                          -                             -                                                               
    SP3:                          -                                                                                             
    SP4:        -     -     -                                                                                                   

    About this scheme

    ECgene alternative splicing isoforms for ECD

    1 Ensembl transcript including schematic representation:
    ENST00000372979  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ECD expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ECD

    1 / 2 / 3

    3 probe-sets matching ECD gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    33746_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 D88208 1.00 1.00 1.00 1

    203013_at2, 3 U133-A 1 1.00 1.00 -- -- NM_007265 0.60 1.00 0.82 1

    203013_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  ECD for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGAGTGCAGC

    SOURCE GeneReport for Unigene cluster: Hs.631822

    UniProtKB/Swiss-Prot: SGT1_HUMAN, O95905
    Tissue specificity: Highly expressed in muscle and heart

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ECD gene from 5/15 species (see all 15 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    ECD1   -- ecdysoneless homolog (Drosophila) 88.91(n)
    86.78(a)
    479244  XM_536386.2  XP_536386.1 
    chimpanzee
    (Pan troglodytes)
    ECD1   -- ecdysoneless homolog (Drosophila) 97.36(n)
    96.74(a)
    450892  XM_001141366.1  XP_001141366.1 
    cow
    (Bos taurus)
    ECD1   -- ecdysoneless homolog (Drosophila) 89.46(n)
    85.67(a)
    524184  NM_001046271.1  NP_001039736.1 
    mouse
    (Mus musculus)
    Ecd1, 5 145
    ecdysoneless homolog (Drosophila)1, 5 82.23(n)1
    79.5(a)1
    706011  NM_027475.21  NP_081751.11 
     AK0176815  AK0286645  (see all 15)
    chicken
    (Gallus gallus)
    ECD1   -- ecdysoneless homolog (Drosophila) 64.66(n)
    60.79(a)
    423638  XM_421522.2  XP_421522.2 
    About this table        Species with no ortholog for ECD

    ENSEMBL Gene Tree for ECD
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/187 NCBI SNPs in ECD are shown (see all 187 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 72)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 10 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs79086061,2
    A,C,F,H74563969(+) TGAATG/CAGTTT 1 -- ng5111Minor allele frequency- C:0.25EU EA WA NA 978
    rs37365181,2
    A,C,F,H74569140(+) GACTTC/GAGTTA 3 E/Q mis1 ese314Minor allele frequency- G:0.24EA NA EU WA 2612
    rs38126191,2
    A,C,F,H74593568(+) CAAACC/TGAGTG 3 R/Q mis1 ese317Minor allele frequency- T:0.25EU EA WA NA 1696
    rs22719031,2
    A,C,F,H74564264(+) TTTACT/CAAATG 1 -- ng519Minor allele frequency- C:0.25EA EU WA NA 2018
    rs22719041,2
    A,C,F,H74564381(+) TGTGAT/CCGGTG 3 G/D mis1 ese316Minor allele frequency- C:0.19NA EU EA WA 1472
    rs125713901,2
    C,F,H74563938(+) atgttA/Gtgcct 1 -- ng514Minor allele frequency- G:0.15EU EA WA 420
    rs70835321,2
    H74569434(+) AAGGTC/TTTCTA 3 D/N mis1 ese34Minor allele frequency- T:0.00EU EA WA 412
    rs70834181,2
    H74569401(+) ATCCTC/TTGGGG 3 E/K mis1 ese34Minor allele frequency- T:0.00EU EA WA 416
    rs361521341,2
    F74566670(+) ACTCAT/CTAGGC 3 S/N mis11Minor allele frequency- C:0.11NA 70
    --
    rs122464841,2
    A74599715(+) taaatG/Ttatta 1 -- ng31 tfbs30--------
    About this table

    HapMap Linkage Disequilibrium images for ECD (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/15 PubMed articles for ECD gene (see all 15 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 11319 HGNC: 17029 AceView: HSGT1 Ensembl:ENSG00000122882 euGenes: HUgn11319
    ECgene: ECD H-InvDB: ECD
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ECD:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for ECD-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for ECD Antibodies for ECD
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ECD Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

     Recombinant Proteins (HSGT1)
     Antibodies (HSGT1)
     Chimera RNAi (HSGT1)
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Recombinant Proteins
    (SGT1)
     Antibodies (SGT1)
     Search OriGene for ECD
     Search Tocris compounds for ECD




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for ECD

    GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

    Random Gene From GIFtS : about GIFtS

    Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

    Developed at the Crown Human Genome Center & Weizmann Institute of Science



    The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
    ECD Gene at Home site.
    Version 2.41.1
    server5.xennexinc.com