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EBF2 Gene

protein-coding   GIFtS: 43

GC08M025757
early B-cell factor 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
COE2 1, 2, 3
EBF-2 2, 3
FLJ11500 1, 2
O/E-3 2
OE-3 2
Descriptions
Collier, Olf and EBF 2 2
OLF-1/EBF-LIKE 3 2
early B-cell factor 2 2, 3
metencephalon-mesencephalnon-olfactory transcription factor
1 2
transcription factor COE2 2
External Ids
HGNC: 190901
Entrez Gene: 646412
UniProtKB: Q9HAK23
Ensembl: ENSG000002218187
Search outside databases for aliases for EBF2 gene

Previous GC identifers: GC08M025474 GC08M026054 GC08M025723

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for EBF2:
EBF2 belongs to the conserved Olf/EBF family (see MIM 164343) of helix-loop-helix transcription
factors (Wang et al., 2002 [PubMed 12139918]).[supplied by OMIM]

UniProtKB/Swiss-Prot: COE2_HUMAN, Q9HAK2
Function: Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL,
TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the
Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence
5'-ATTCCCNNGGGAATT-3' (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the EBF2 gene  

Entrez Gene cytogenetic band: 8p21.2   Ensembl cytogenetic band:  8p21.2   HGNC cytogenetic band: 8p21.1

EBF2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M025757:     (about GC identifiers)

Start:
25,757,490 bp from pter
End:
25,958,309 bp from pter
Size:
200,820 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000008.9  NT_023666.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: COE2_HUMAN, Q9HAK2 (See protein sequence)
Recommended Name: Transcription factor COE2  
Size: 575 amino acids; 62650 Da
Subunit: Forms either a homodimer or a heterodimer with a related family member (By similarity)
Subcellular location: Nucleus (Potential)
Secondary accessions: A0PJM4 A6NMF7 Q6DK36 Q6IS86 Q6ISA4

REFSEQ proteins: NP_073150.2  

ENSEMBL proteins: 
ENSP00000386178 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Sigma-Aldrich AQUA Peptides for EBF2  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (EBF2)
Browse Abnova for Human Recombinant Proteins
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
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Antibodies for EBF2: 
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Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for EBF2
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Antibodies from Abcam (EBF2), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for EBF2

Assays for EBF2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5 InterPro domains/families:
 IPR001092 HLH_basic
 IPR002909 IPT_TIG_rcpt
 IPR018350 Transcription_factor_COE_CS
 IPR013783 Ig-like_fold
 IPR003523 Transcription_factor_COE


   GeneDecks  EBF2 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9HAK2

ProtoNet protein and cluster: Q9HAK2

3 Blocks protein families:
IPB001092 Basic helix-loop-helix dimerization domain bHLH
IPB002909 Cell surface receptor IPT/TIG
IPB003523 Transcription factor COE


UniProtKB/Swiss-Prot: COE2_HUMAN, Q9HAK2
Similarity: Belongs to the COE family
Similarity: Contains 1 IPT/TIG domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EBF2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): XM_931923

              Applied Biosystems Silencer® siRNAs for EBF2

              Sigma-Aldrich siRNA for EBF2  
                     Sigma-Aldrich shRNA for EBF2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_022659
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_022659
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_022659 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_022659

UniProtKB/Swiss-Prot: COE2_HUMAN, Q9HAK2
Function: Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL,
TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the
Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence
5'-ATTCCCNNGGGAATT-3' (By similarity)

12 MGI mutant phenotypes (inferred from 5 alleles(MGI details for Ebf2):

adipose tissuebehavior/neurologicalendocrine/exocrine glandgrowth/sizelethality-postnatal
limbs/digits/tailnervous systemnormalreproductive systemrespiratory system
skeletontaste/olfaction

5 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003677 DNA binding IEA--
GO:0005515 protein binding IEA--
GO:0008270 zinc ion binding IEA--
GO:0030528 transcription regulator activity IEA--
GO:0046872 metal ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


  --

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006355 regulation of transcription, DNA-dependent IEA--
GO:0007275 multicellular organismal development IEA--
GO:0045941 positive regulation of transcription IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for EBF2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EBF2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): XM_931923

              Sigma-Aldrich siRNA for EBF2  
                     Sigma-Aldrich shRNA for EBF2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_022659  

REFSEQ mRNAs for EBF2 gene: 

NM_022659.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_022659  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_022659
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_022659
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_022659 

Additional cDNA sequence: 

AK001144.1 AK021562.1 AK304372.1 AY700779.1 BC069665.1 BC069726.1 BC069747.1 BC069768.1 
BC074794.2 BC113478.1 BC113504.1 BC137106.1 BC137107.1 

3 DOTS entries:

DT.75105086  DT.99941220  DT.70102522 

24/30 AceView cDNA sequences (see all 30 ):

AU117744 BC069768 AI457889 BC069726 AK021562 NM_022659 BX282733 BQ878633 
BC074794 BI488508 BC069747 AI200996 AK093878 BQ889735 BC069665 AL706446 
AA911958 AY700779 AA311763 AI016316 AI744592 AW962848 AU144494 W92336 

highest scoring ESTs for EBF2:

AI744592 AK021562 AU117744 BC069665 BC069726 BC069747 BC069768 AK001144 AL701879 AL706446 

Unigene Cluster for EBF2:

Early B-cell factor 2
Hs.710674  [show with all ESTs]
Unigene Representative Sequence: AK001144


GeneLoc Exon Structure

1 Ensembl transcript including schematic representation:
ENST00000408929  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

EBF2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for EBF2

1 / 2 / 3

4 probe-sets matching EBF2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

62452_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AI744592 0.80 1.00 0.91 1

220392_at2, 3 U133-A 1 1.00 1.00 -- -- NM_022659 0.40 1.00 0.76 1

1562477_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

220392_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.710674
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for EBF2 gene from 5/9 species (see all 9 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
EBF21   -- early B-cell factor 2 92.52(n)
99.48(a)
477375  XM_853413.1  XP_858506.1 
chimpanzee
(Pan troglodytes)
EBF21   -- early B-cell factor 2 99.59(n)
99.65(a)
464067  XM_001161196.1  XP_001161196.1 
rat
(Rattus norvegicus)
Ebf21   -- early B-cell factor 2 89.87(n)
99.64(a)
361060  XM_341343.3  XP_341344.3 
mouse
(Mus musculus)
Ebf21, 5 14 (30.50 cM)5
early B-cell factor 21, 5 89.91(n)1
99.48(a)1
135921  NM_010095.31  NP_034225.11 
 AK0530135  AK1392625  (see all 11)
chicken
(Gallus gallus)
EBF21   -- early B-cell factor 2 84.06(n)
98.43(a)
395176  XM_417675.2  XP_417675.2 
About this table        Species with no ortholog for EBF2

ENSEMBL Gene Tree for EBF2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for EBF2 gene
EBF32  EBF42  EBF12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/1304 NCBI SNPs in EBF2 are shown (see all 1304 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 440)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 8 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs45388921,2
A,C,F,H25757689(+) AACAAA/GTAGTA 1 -- ut317Minor allele frequency- G:0.00EU EA WA NA 548
rs38290111,2
A,C,F,H25757578(-) CAGCAC/TGGAAG 1 -- ut3111Minor allele frequency- T:0.27NA EU EA WA 922
rs93143181,2
C,F,H25960305(+) GATTCG/ACTTGA 1 -- ng318Minor allele frequency- A:0.38EU EA WA 838
rs170544771,2
C,F,H25764048(+) ATTGCC/TGCTGG 1 G/S mis111Minor allele frequency- T:0.03NA EU EA WA 980
rs119868941,2
C,F,H25757815(+) ACTACG/ATCAAT 1 -- ut314Minor allele frequency- A:0.03EU EA WA 418
--
rs9011761,2
A,C25959095(-) CAGCCC/TGCAGA 1 -- ng311Minor allele frequency- T:0.00MN 184
--
rs9011751,2
A,C25959096(-) CCAGCC/TCGCAG 1 -- ng311Minor allele frequency- T:0.00MN 184
rs10776691,2
C,F25959370(-) CCTTCT/ATTCAT 1 -- ng311Minor allele frequency- A:0.15MN 184
rs70076771,2
A,C25958418(+) CCCAAA/GTTTGA 1 -- ng310--------
rs10776701,2
C,F25959300(-) TCCAGG/AAGACC 1 -- ng311Minor allele frequency- A:0.15MN 184
About this table

HapMap Linkage Disequilibrium images for EBF2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 609934

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10 PubMed articles for EBF2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 64641 HGNC: 19090 AceView: EBF2 Ensembl:ENSG00000221818 euGenes: HUgn64641
ECgene: EBF2 H-InvDB: EBF2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for EBF2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
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 Predesigned and custom siRNAs for EBF2 Antibodies for EBF2
 Explore super-pooled esiRNAs Proteins for EBF2
 Lentivirus-delivered shRNAs for EBF2 Browse small molecules at Sigma-Aldrich
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 Search Novus for antibodies for EBF2

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