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EBF1 Gene

protein-coding   GIFtS: 54

GC05M158059
early B-cell factor 1
(Previous name: early B-cell factor )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: EBF)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
COE1 2, 3
EBF 2, 3
FLJ39389 2
FLJ41763 2
O/E-1 2, 3
OE-1 3
OLF1 1, 2
Descriptions
Collier, Olf and EBF transcription factor 1 2
Early B-cell factor 1, 2, 3
early B-cell factor 1 2
olfactory neuronal transcription factor 1 2
External Ids
HGNC: 31261
Entrez Gene: 18792
UniProtKB: Q9UH733
Ensembl: ENSG000001643307
Search outside databases for aliases for EBF1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: COE1_HUMAN, Q9UH73
Function: Transcriptional activator which recognizes variations of the palindromic sequence
5'-ATTCCCNNGGGAATT-3' (By similarity)

Gene Wiki entry for EBF1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q34   Ensembl cytogenetic band:  5q33.3   HGNC cytogenetic band: 5q34

EBF1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M158059:     (about GC identifiers)

Start:
158,058,006 bp from pter
End:
158,459,347 bp from pter
Size:
401,342 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000005.8  NT_023133.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: COE1_HUMAN, Q9UH73 (See protein sequence)
Recommended Name: Transcription factor COE1  
Size: 591 amino acids; 64464 Da
Subunit: Forms either a homodimer or a heterodimer with a related family member (By similarity).
Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes
Subcellular location: Nucleus (Potential)
Secondary accessions: Q8IW11
Alternative splicing: 2 isoforms:  Q9UH73-1   Q9UH73-2   

REFSEQ proteins: NP_076870.1  

ENSEMBL proteins: 
ENSP00000322898 ENSP00000370029 ENSP00000337140 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Sigma-Aldrich AQUA Peptides for EBF1  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (EBF1)
Human Recombinant Proteins from Abnova (EBF1)
              OriGene Purified Recombinant Human Protein: EBF1 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
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Antibodies for EBF1: 
Browse Antibodies Central at Invitrogen
Millipore Mono- and Polyclonal Antibodies for the study of EBF1
Sigma-Aldrich Antibodies for EBF1
R&D Systems Antibodies for EBF1 (EBF-1)
Antibodies from Abcam (EBF1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (EBF1)
Novus Biologicals Antibodies for EBF1

Assays for EBF1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR001092 HLH_basic
 IPR002909 IPT_TIG_rcpt
 IPR018350 Transcription_factor_COE_CS
 IPR003523 Transcription_factor_COE


   GeneDecks  EBF1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9UH73

ProtoNet protein and cluster: Q9UH73

3 Blocks protein families:
IPB001092 Basic helix-loop-helix dimerization domain bHLH
IPB002909 Cell surface receptor IPT/TIG
IPB003523 Transcription factor COE


UniProtKB/Swiss-Prot: COE1_HUMAN, Q9UH73
Similarity: Belongs to the COE family
Similarity: Contains 1 IPT/TIG domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EBF1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EBF1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024007

              Applied Biosystems Silencer® siRNAs for EBF1

              Sigma-Aldrich siRNA for EBF1  
                     Sigma-Aldrich shRNA for EBF1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for EBF1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024007
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024007
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_024007 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_024007

UniProtKB/Swiss-Prot: COE1_HUMAN, Q9UH73
Function: Transcriptional activator which recognizes variations of the palindromic sequence
5'-ATTCCCNNGGGAATT-3' (By similarity)

9 MGI mutant phenotypes (inferred from 1 allele(MGI details for Ebf1):

adipose tissuegrowth/sizehematopoietic systemimmune systemlife span-post-weaning/aging
limbs/digits/tailliver/biliary systemnervous systemskeleton

5 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003677 DNA binding IEA--
GO:0005515 protein binding IDA14594818
GO:0008270 zinc ion binding IEA--
GO:0030528 transcription regulator activity IEA--
GO:0046872 metal ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


2 Millipore Pathways for EBF1
 Selected targets of C/EBPbeta
 Selected targets of C/EBPalpha

   GeneDecks  EBF1 for the pathways selected above  
About GeneDecksing

 Gene Network CentralTM Interacting Genes and Proteins Network for  EBF1 


5/23 Interacting proteins for EBF1 (ENSP000003228983) via UniProtKB, MINT, and/or STRING (see all 23 )
InteractantInteraction Details
GeneCardExternal ID(s)
ZNF423ENSP000002623833STRING (score=.994)
TCF3ENSP000002629653STRING (score=.98)
SPI1ENSP000003677993STRING (score=.967)
IKZF1ENSP000003316143STRING (score=.963)
IKZF3ENSP000002930683STRING (score=.961)
About this table

2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006355 regulation of transcription, DNA-dependent IEA--
GO:0007275 multicellular organismal development IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for EBF1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EBF1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (EBF1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024007

              Sigma-Aldrich siRNA for EBF1  
                     Sigma-Aldrich shRNA for EBF1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_024007  

REFSEQ mRNAs for EBF1 gene: 

NM_024007.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_024007  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024007
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024007
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_024007 

Additional cDNA sequence: 

AF208502.1 AK021803.1 AK096708.1 AK123757.1 AK289712.1 AK303698.1 AK304438.1 BC019927.1 
BC038805.1 BC041178.1 

10 DOTS entries:

DT.97839542  DT.309797  DT.206057  DT.40198193  DT.97839541  DT.101957176  DT.100683327  DT.120818321 
DT.95069897  DT.95356530 

24/105 AceView cDNA sequences (see all 105 ):

AA910081 BM932023 AK021803 BX282724 BM802733 AA746092 AA427900 AI478562 
AA904035 AI493206 AA669979 H88863 AU145682 BM685055 BQ423155 AA761874 
BQ430921 AA814680 AI458258 AI472207 CB990370 AW303324 AA743152 AU119304 

highest scoring ESTs for EBF1:

AF208502 AI381930 BC038805 BC041178 BQ227494 BQ430921 BQ438923 BU597395 BX282724 CB989550 

Unigene Cluster for EBF1:

Early B-cell factor 1
Hs.573143  [show with all ESTs]
Unigene Representative Sequence: NM_024007


GeneLoc Exon Structure

3 Ensembl transcripts including schematic representations:
ENST00000313708  ENST00000380654  ENST00000336546  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

EBF1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for EBF1

1 / 2 / 3

11 probe-sets matching EBF1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

57550_at2, 3 U95-B 1 1.00 1.00 0.91 0.93 W73890 0.20 1.00 0.72 1

78945_at2, 3 U95-E 2 1.00 0.97 -0.16 0.43 AI381930 0.80 1.00 0.91 1

61699_at2, 3 U95-C 1 0.94 1.00 0.82 1.07 AA488889 0.40 1.00 0.76 1

227646_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

232204_at2, 3 U133-B 1 1.00 1.00 -- -- AF208502 0.80 1.00 0.91 1

229487_at2, 3 U133-B 1 1.00 1.00 -- -- W73890 0.20 1.00 0.72 1

233261_at*2, 3 U133-B 1 1.00 1.00 -- -- AU145682 0.40 1.00 0.76 1

227646_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

232204_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

229487_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

233261_at*2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TGCAGTGGTT

SOURCE GeneReport for Unigene cluster: Hs.573143
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for EBF1 gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
EBF11   -- early B-cell factor 1 96(n)
99.32(a)
479312  XM_848259.1  XP_853352.1 
chimpanzee
(Pan troglodytes)
EBF11   -- early B-cell factor 1 99.77(n)
100(a)
462229  XM_001140192.1  XP_001140192.1 
rat
(Rattus norvegicus)
Ebf11   -- early B-cell factor 1 94.27(n)
99.82(a)
116543  NM_053820.1  NP_446272.1 
mouse
(Mus musculus)
Ebf11, 5 11 (20.00 cM)5
early B-cell factor 11, 5 94.08(n)1
100(a)1
135911  NM_007897.21  NP_031923.11 
 AA2503585  AK0129645  (see all 19)
chicken
(Gallus gallus)
EBF11   -- early B-cell factor 1 86.63(n)
98.48(a)
395512  NM_204752.1  NP_990083.1 
About this table        Species with no ortholog for EBF1

ENSEMBL Gene Tree for EBF1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for EBF1 gene
EBF32  EBF22  EBF42  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/1850 NCBI SNPs in EBF1 are shown (see all 1850 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 679)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 5 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs49213101,2
C,F,H158461210(+) AATGTG/ACCCCA 1 -- ng314Minor allele frequency- A:0.03EU EA WA 420
rs12958471,2
H158056451(-) TGTTTT/GGTTAC 1 -- ut315Minor allele frequency- G:0.00MN EU EA WA 598
rs12767071,2
H158058063(-) TCTTTT/GGTATT 1 -- ut314Minor allele frequency- G:0.00EU EA WA 418
rs13682981,2
A,C,F,H158137003(-) ATTTAC/TACAGG 1 Y/Y syn112Minor allele frequency- T:0.35NA EU EA WA 1056
rs20096121,2
A,C,F,H,O158260568(+) GCAACC/GTTACA 1 -- int17Minor allele frequency- G:0.10EU EA WA NA 560
rs15416491,2
A,C,F,H,O158118880(-) TGGTGA/TCAATA 1 -- int115Minor allele frequency- T:0.17NA EU EA WA 1340
rs13636191,2
A,C,F,H,O158231289(+) CATGTC/ATTTCC 1 -- int114Minor allele frequency- A:0.21NS MN EU EA WA 1206
rs19787061,2
A,C,F,H,O158206649(+) ACTGAT/CTTCAA 1 -- int111Minor allele frequency- C:0.44NA EU EA WA 982
rs15416481,2
A,C,F,H,O158119047(-) ATGAGC/TCGTAC 1 -- int116Minor allele frequency- T:0.06NA EA MN NS EU WA 1144
rs13682971,2
A,C,F,H,O158187624(+) TAGTCA/TGTTCT 1 -- int110Minor allele frequency- T:0.43EU EA WA NA 814
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HapMap Linkage Disequilibrium images for EBF1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 164343

1 Novoseek disease relationship for EBF1 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
plasmacytoma 62.36 1 8693297 (1)
About this table

Human Genome Epidemiology Navigator: EBF1 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/59 PubMed articles for EBF1 gene (see all 59 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 1879 HGNC: 3126 AceView: EBF Ensembl:ENSG00000164330 euGenes: HUgn1879
ECgene: EBF1 H-InvDB: EBF1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for EBF1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for EBF1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for EBF1 Antibodies for EBF1
 Explore super-pooled esiRNAs Proteins for EBF1
 Lentivirus-delivered shRNAs for EBF1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

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 Custom Service for Rabbit Pab from Full-length Protein
  
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 Recombinant Proteins
(EBF1)
 Antibodies (EBF1)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for EBF1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for EBF1

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