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Aliases & Descriptions for EBAG9
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases EB9 1 , 2 , 5 PDAF 2 RCAS1 1 , 2 , 3 , 5
Descriptions Cancer-associated surface antigen RCAS1 3 Estrogen receptor-binding fragment-associated gene 9 protein 3 cancer associated surface antigen 2 estrogen receptor binding site associated antigen 9 2 estrogen receptor binding site associated, antigen, 9 2
Search outside databases for aliases for EBAG9 genePrevious GC identifers: GC08P109536 GC08P110619 GC08P110220 GC08P110508
Summaries for EBAG9 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for EBAG9 : This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen ismediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that isexpressed at high frequency in a variety of cancers. Two transcript variants differing in the5' UTR, but encoding the same protein, have been identified for this gene. [provided byRefSeq] UniProtKB/Swiss-Prot: RCAS1_HUMAN, O00559 Function : May participate in suppression of cell proliferation and induces apoptotic cell deaththrough activation of interleukin-1-beta converting enzyme (ICE)-like proteases
Gene Wiki entry for EBAG9
Genomic Location for EBAG9
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the EBAG9 gene Entrez Gene cytogenetic band: 8q23 Ensembl cytogenetic band: 8q23.2 HGNC cytogenetic band: 8q23 EBAG9 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 8 GeneLoc Exon Structure
GeneLoc location for GC08P110621:
(about GC identifiers )
Start:
110,621,105 bp from pter
End:
110,646,568 bp from pter
Size:
25,464 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000008.9 NT_008046.15 Proteins for EBAG9
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: RCAS1_HUMAN, O00559 (See
protein sequence )Recommended Name: Receptor-binding cancer antigen expressed on SiSo cells Size : 213 amino acids; 24377 Da
Subunit : Homodimer
Subcellular location : Golgi apparatus membrane; Single-pass type III membrane protein.Note=According to PubMed:10426319, it also exists as a soluble form which has the same biologicalactivities. The existence of such soluble form is however uncertain
Miscellaneous : May serve as a prognostic marker for cancers such as adenocarcinomas of the lung andbreast cancers
Caution : It was initially reported to be a ligand for some putative receptor present on T-, B-,natural killer (NK) cells and various human cell lines. However, PubMed:12672804 showed that itdoes not bind any receptor
Secondary accessions : Q6IB20 Q9BS76
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins (2 alternative transcripts):
NP_004206.1 NP_936056.1 ENSEMBL proteins: ENSP00000337675 ENSP00000276658 ENSP00000379131 Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: EBAG9 5 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for EBAG9: Assays for EBAG9:
Protein
Domains/ Families for EBAG9(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry O00559 ProtoNet protein and cluster: O00559
UniProtKB/Swiss-Prot: RCAS1_HUMAN, O00559 Domain : The coiled coil domain is necessary for the homodimerization
Gene Function for EBAG9
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3 ): NM_004215 Applied Biosystems Silencer ® siRNAs for EBAG9 Sigma-Aldrich siRNA for EBAG9 Sigma-Aldrich shRNA for EBAG9 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_004215                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_004215                                  untagged cDNA clones in CMV expression vector (see all 3 ): BC017729  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_004215 UniProtKB/Swiss-Prot: RCAS1_HUMAN, O00559 Function : May participate in suppression of cell proliferation and induces apoptotic cell deaththrough activation of interleukin-1-beta converting enzyme (ICE)-like proteases Induction : By estrogen
1 Gene Ontology (GO) molecular function term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0016505 apoptotic protease activator activity
NAS 10426319
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Pathways & Interactions for EBAG9
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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Gene Network CentralTM Interacting Genes and Proteins Network for EBAG9 5/6 Interacting proteins for EBAG9 (ENSP00000276658 3 ) via UniProtKB, MINT, and/or STRING (see all 6
)About this table 2 Gene Ontology (GO) biological process terms (links to tree view) :
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Drugs & Compounds for EBAG9 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for EBAG9
Transcripts for EBAG9(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
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shRNA from
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OriGene ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3 ): NM_004215 Sigma-Aldrich siRNA for EBAG9 Sigma-Aldrich shRNA for EBAG9 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_004215 NM_198120
REFSEQ mRNAs for EBAG9 gene (2 alternative transcripts): NM_004215.3 NM_198120.1
Applied Biosystems TaqMan ® Gene Expression Assays: NM_004215 NM_198120
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_004215                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_004215                                  untagged cDNA clones in CMV expression vector (see all 3 ): BC017729  
Additional cDNA sequence: AB007619.1 AF006265.1 AK290651.1 AK308950.1 AY515724.1 AY653072.1 BC005249.1 BC017729.1 BC022506.1 CR456984.1 CR593368.1 CR613560.1 CR621221.1
6 DOTS entries : DT.95092068 DT.99936322 DT.91731766 DT.100781926 DT.120635978 DT.91731760
24/159 AceView cDNA sequences (see all 159
):AI803757 BU785578 AW271599 AI168650 BM272715 AI698984 BM875293 AA479310 BQ549341 BU790091 BX281135 AW272900 BU785557 AW572264 CD108096 AA868695 CA450148 BM313192 BM692472 AI619447 AA812215 BU731215 AI333647 AA632054
highest scoring ESTs for EBAG9 :AB007619 AA027768 AA143053 AA255434 AA479310 AA602624 AA632054 AA757112 AA765519 AA766708
Unigene Cluster for EBAG9: Estrogen receptor binding site associated, antigen, 9 Hs.409368 [show with all ESTs ] Unigene Representative Sequence: BC022506 GeneLoc Exon Structure 3 Ensembl transcripts including schematic representations : ENST00000337573
ENST00000276658
ENST00000395785
Expression for EBAG9
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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EBAG9 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for EBAG9 1 / 2 / 3
5 probe-sets matching EBAG9 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: AAAATGACATSOURCE GeneReport for Unigene cluster: Hs.409368 Expression variation in blood from EXPOLDB for EBAG9
UniProtKB/Swiss-Prot: RCAS1_HUMAN, O00559 Tissue specificity : Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle andheart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. Theprotein is not detected in any of the above organs
Orthologs for EBAG9
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for EBAG9 gene from 5/8 species (see all 8
)
Organism
Gene
Locus
Description
Human Similarity
NCBI accessions
dog (Canis familiaris)
EBAG91
--
estrogen receptor binding site associated, antigen, more
94.05(n) 96.24(a)
403500 NM_001002996.1 NP_001002996.1
chimpanzee (Pan troglodytes)
EBAG91
--
estrogen receptor binding site associated, antigen, more
99.69(n) 100(a)
464339 XM_001136659.1 XP_001136659.1
rat (Rattus norvegicus)
Ebag91
--
estrogen receptor binding site associated, antigen, more
91.08(n) 94.37(a)
299864 NM_001009665.1 NP_001009665.1
mouse (Mus musculus)
Ebag91 , 5
155
estrogen receptor-binding fragment-associated gene more 1, 5
91.08(n) 1 97.65(a) 1
55960 1 NM_019480.4 1 NP_062353.3 1 AA896719 5 AF076524 5 (see all 15 )
chicken (Gallus gallus)
EBAG91
--
estrogen receptor binding site associated, antigen, more
79.66(n) 81.36(a)
420276 NM_001030949.1 NP_001026120.1
About this table Species with no ortholog for EBAG9 ENSEMBL Gene Tree for EBAG9 Paralogs for EBAG9 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Pseudogenes 1 related pseudogene
SNPs/Variants for EBAG9 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for EBAG9 (up to first 250kb)
Disorders & Mutations for EBAG9
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
Novoseek , PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 605772 UniProtKB/Swiss-Prot: RCAS1_HUMAN, O00559
Defects in EBAG9 may be a cause of breast cancers and adenocarcinomas of the lung. It ispresent and overexpressed in many patients suffering from breast carcinomas, its level ofexpression correlates with tumor grade, suggesting that it may be involved in cancer immuneescape. According to PubMed:12672804, it is however not directly a tumor-associated antigen, butit rather modulates surface expression of tumor-associated O-linked glycan Tn when it isoverexpressed, suggesting that it contributes indirectly to the antigenicity of tumor cells
10/32 Novoseek disease relationships for EBAG9 gene (see all 32
)
Disease
Score
Articles
PubMed IDs for Articles with Shared Sentences (# sentences)
tumor escape
71.57
4
16762017 (1), 11102892 (1), 14740005 (1), 15016327 (1)
cervical adenocarcinoma
60.67
2
17981616 (1), 11104614 (1)
cancer
56.56
89
17981616 (3), 19175908 (2), 11374862 (2), 17273743 (2) (see all 51 )
nasal polyps
43.76
6
16551346 (3), 17187008 (2), 14740005 (1)
gallbladder carcinoma
42.17
2
15460847 (1), 16039043 (1)
carcinoma
41.58
27
12457035 (4), 15460847 (2), 12971966 (2), 11562743 (1) (see all 11 )
tumors
41.09
112
14534714 (7), 14981953 (6), 18007080 (6), 15254686 (4) (see all 33 )
adenocarcinoma
41.08
18
11992411 (3), 11104614 (2), 11102892 (2), 11562743 (1) (see all 11 )
invasive cancer
39.46
5
16112176 (3)
metastasis
39.30
21
17727777 (3), 17187007 (2), 16112176 (2), 19175908 (1) (see all 14 )
About this table Genetic Association Database: EBAG9
Medical News for EBAG9 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for EBAG9 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/106 PubMed articles for EBAG9 gene (see all 106
): Overrepresentation of the EBAG9 gene at 8q23 associated with early-stage breast cancers. (PubMed id 11705872) 1, 3, 6 Tsuneizumi M....Nakamura Y. (2001) Isolation of estrogen-responsive genes with a CpG island library. (PubMed id 9418891) 1, 3, 4 Watanabe T....Muramatsu M. (1998) Inhibition of cell growth and induction of apoptotic cell death by the human tumor-associated antigen RCAS1. (PubMed id 10426319) 1, 3, 4 Nakashima M.... Watanabe T. (1999) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983) 3, 4 Olsen J.V....Mann M. (2006) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. (PubMed id 15592455) 3, 4 Rush J.... Comb M.J. (2005) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. (PubMed id 15144186) 3, 4 Brill L.M....Peters E.C. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) The Golgi protein RCAS1 controls cell surface expression of tumor- associated O-linked glycan antigens. (PubMed id 12672804) 3, 4 Engelsberg A....Rehm A. (2003) RCAS1 expression: a potential prognostic marker for adenocarcinomas of the lung. (PubMed id 12138241) 3, 4 Oizumi S.... Dosaka-Akita H. (2002) RCAS1 is associated with ductal breast cancer progression. (PubMed id 12054692) 3, 4 Rousseau J.... Guerette B. (2002)
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Genome Databases showing EBAG9
(According to
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HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Specialized Databases showing EBAG9 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for EBAG9 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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