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EARS2 Gene

protein-coding   GIFtS: 50

GC16M023440
glutamyl-tRNA synthetase 2, mitochondrial (putative)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.1.1.17 3
GluRS 3
KIAA1970 1, 2, 3
MSE1 1, 2
Descriptions
Glutamate--tRNA ligase 3
glutamate tRNA ligase 2, mitochondrial 2
glutamyl-tRNA synthetase 2 2
glutamyl-tRNA synthetase 2, mitochondrial (putative) 2
External Ids
HGNC: 294191
Entrez Gene: 1244542
UniProtKB: Q5JPH63
Ensembl: ENSG000001033567
Search outside databases for aliases for EARS2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: SYEM_HUMAN, Q5JPH6
Function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is
first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) (By
similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the EARS2 gene  

Entrez Gene cytogenetic band: 16p12.1   Ensembl cytogenetic band:  16p12.1   HGNC cytogenetic band: 16p12.3

EARS2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M023440:     (about GC identifiers)

Start:
23,440,835 bp from pter
End:
23,476,197 bp from pter
Size:
35,363 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000016.8  NT_010393.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SYEM_HUMAN, Q5JPH6 (See protein sequence)
Recommended Name: Probable glutamyl-tRNA synthetase, mitochondrial precursor  
Size: 523 amino acids; 58689 Da
Subcellular location: Mitochondrion matrix (By similarity)
Secondary accessions: B3KTT2 Q8TF31

REFSEQ proteins: NP_001077083.1  

ENSEMBL proteins: 
ENSP00000343488 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (KIAA1970)
Human Recombinant Proteins from Abnova (KIAA1970)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005739 mitochondrion IEA--
GO:0005759 mitochondrial matrix IEA--
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Antibodies for EARS2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (KIAA1970), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (KIAA1970)
Novus Biologicals Antibodies for EARS2

Assays for EARS2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5 InterPro domains/families:
 IPR001412 aa-tRNA-synth_I_CS
 IPR004527 Glu-tRNA-synth_Ic_bac/mito
 IPR008925 aa-tRNA-synth_I_codon-bd
 IPR000924 Glu/Gln-tRNA-synth_Ic
 IPR014729 Rossmann-like_a/b/a_fold


   GeneDecks  EARS2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q5JPH6

ProtoNet protein and cluster: Q5JPH6

1 Blocks protein family: IPB000924 Glutamyl-tRNA synthetase signature

UniProtKB/Swiss-Prot: SYEM_HUMAN, Q5JPH6
Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EARS2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NR_003501

              Applied Biosystems Silencer® siRNAs for EARS2

              Sigma-Aldrich siRNA for EARS2  
                     Sigma-Aldrich shRNA for EARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001083614
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001083614
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001083614 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001083614

UniProtKB/Swiss-Prot: SYEM_HUMAN, Q5JPH6
Function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is
first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) (By
similarity)
Catalytic activity: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Enzyme Number (IUBMB): EC 6.1.1.17 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0004818 glutamate-tRNA ligase activity IEA--
GO:0005524 ATP binding IEA--
GO:0016874 ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


2 Sigma-Aldrich "Your Favorite Gene" Pathways for  EARS2  (Your Favorite Gene powered by Ingenuity) 
 Glutamate Metabolism
 Aminoacyl-tRNA Biosynthesis

   GeneDecks  EARS2 for the pathways selected above  
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3 Kegg Pathways  (Kegg details for EARS2):
 hsa00251 Glutamate metabolism
 hsa00860 Porphyrin and chlorophyll metabolism
 hsa00970 Aminoacyl-tRNA biosynthesis

   GeneDecks  EARS2 for the pathways selected above  
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5/37 Interacting proteins for EARS2 (ENSP000003434883) via UniProtKB, MINT, and/or STRING (see all 37 )
InteractantInteraction Details
GeneCardExternal ID(s)
GLULENSP000003565373STRING (score=.907)
GLUD2ENSP000003275893STRING (score=.905)
GLUD1ENSP000002778653STRING (score=.904)
GOT2ENSP000002452063STRING (score=.904)
GOT1ENSP000003595393STRING (score=.901)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006424 glutamyl-tRNA aminoacylation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for EARS2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (EARS2)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NR_003501

              Sigma-Aldrich siRNA for EARS2  
                     Sigma-Aldrich shRNA for EARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001083614  

REFSEQ mRNAs for EARS2 gene: 

NM_001083614.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001083614  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001083614
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001083614
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001083614 

Additional cDNA sequence: 

AB075850.1 AF088053.1 AK095998.1 AL832489.1 BC020592.1 BC040013.1 BX648129.1 CR613598.1 

18 DOTS entries:

DT.100844821  DT.448238  DT.100781219  DT.120691069  DT.100008435  DT.100027545  DT.95275717  DT.95099257 
DT.120691009  DT.120691084  DT.91713220  DT.95199993  DT.95218806  DT.100781217  DT.120690960  DT.120690994 
DT.120691020  DT.97784815 

highest scoring ESTs for EARS2:

AB075850 AI547137 AI929390 AK095998 AL520724 AL559488 AL832489 BC040013 BE242636 BE612832 

Unigene Cluster for EARS2:

Glutamyl-tRNA synthetase 2, mitochondrial (putative)
Hs.620541  [show with all ESTs]
Unigene Representative Sequence: NM_001083614


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for EARS2

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12
SP1:                                                                                          
SP2:        -     -     -                                                                     
SP3:                                                                                          
SP4:                    -                                                                     

About this scheme

ECgene alternative splicing isoforms for EARS2

1 Ensembl transcript including schematic representation:
ENST00000341597  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

EARS2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for EARS2

1 / 2 / 3

7 probe-sets matching EARS2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
49397_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI547137 0.60 1.00 0.82 1

227374_at2, 3 U133-B 1 1.00 1.00 -- -- AA833716 0.20 0.50 0.38 1

225993_at2, 3 U133-B 1 1.00 1.00 -- -- AL520723 0.20 1.00 0.72 1

1563796_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1555437_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

227374_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

225993_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  EARS2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.620541
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for EARS2 gene from 5/18 species (see all 18 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
EARS21   -- glutamyl-tRNA synthetase 2, mitochondrial (putative) 86.62(n)
86.04(a)
479807  XM_536934.2  XP_536934.2 
chimpanzee
(Pan troglodytes)
EARS21   -- glutamyl-tRNA synthetase 2, mitochondrial (putative) 99.04(n)
99.04(a)
744351  XM_001162700.1  XP_001162700.1 
rat
(Rattus norvegicus)
LOC6852451   -- similar to CG4573-PA 85.08(n)
86.39(a)
685245  XM_001062991.1  XP_001062991.1 
mouse
(Mus musculus)
Ears21, 5 75
glutamyl-tRNA synthetase 2 (mitochondrial)(putative)1, 5 84.83(n)1
85.09(a)1
674171  NM_026140.21  NP_080416.11 
 AK0143245  AK0289955  (see all 15)
chicken
(Gallus gallus)
EARS21   -- glutamyl-tRNA synthetase 2, mitochondrial (putative) 69.45(n)
68.64(a)
427672  NM_001031467.1  NP_001026638.1 
About this table        Species with no ortholog for EARS2

ENSEMBL Gene Tree for EARS2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/186 NCBI SNPs in EARS2 are shown (see all 186 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 66)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 16 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs14681381,2
A,C,F,H,O23443181(-) GATGTG/TTCGGG 1 -- ut319Minor allele frequency- T:0.27MN EU EA WA NA 2228
rs64976711,2
A,C,F,H23444185(+) CATACC/TAGATC 1 S/G mis18Minor allele frequency- T:0.19EU EA WA 838
rs73427351,2
C,F,H23441831(+) aagcgA/Gtcaaa 1 -- ut318Minor allele frequency- G:0.01EU EA WA 838
--
rs97071,2
A,C,F23441599(-) tgctcT/Cggaac 1 -- ut31 ese31Minor allele frequency- C:0.11MN 184
rs133349571,2
C,F,H23441037(+) GAACAA/CCTTTT 1 -- ut314Minor allele frequency- C:0.04EU NA 246
--
rs64976691,2
A,C23441750(+) gcctcC/Gatccc 1 -- ut31 ese30--------
--
rs110745651,2
A,C23440509(+) cttttA/Gtgact 1 -- ng510--------
rs116475571,2
C23442438(+) CTTGGA/GGCCTG 1 -- ut31 ese30--------
--
rs30713791,2
F23442809(-) aaaca-/ACAGA 
        
gattt
1 -- ut314Minor allele frequency- ACAGA:0.21EU NA MN 380
rs118645691,2
H23441973(+) tagaaG/Aaaggc 1 -- ut314Minor allele frequency- A:0.00EU EA WA 390
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HapMap Linkage Disequilibrium images for EARS2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 612799

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

7 PubMed articles for EARS2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 124454 HGNC: 29419 Ensembl:ENSG00000103356 euGenes: HUgn124454 ECgene: EARS2
H-InvDB: EARS2
(According to HUGE)
About This Section

HUGE: KIAA1970
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for EARS2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for EARS2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for EARS2 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for EARS2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

 Recombinant Proteins (KIAA1970)
 Antibodies (KIAA1970)
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 Custom Service for Rabbit Pab from Full-length Protein
  
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 Recombinant Proteins
(KIAA1970)
 Antibodies (KIAA1970)
 Search OriGene for EARS2
 Search Tocris compounds for EARS2




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for EARS2

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