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Aliases & Descriptions for EAF2
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| BM040 1, 2 | | TRAITS 1, 2, 3 | | U19 1, 2 |
| | | Descriptions |
|---|
| ELL associated factor 2 2 | | ELL-associated factor 2 2 | Testosterone-regulated apoptosis inducer and tumor suppressor protein 3 | testosterone regulated apoptosis inducer and tumor suppressor 2 |
|
| | Search outside databases for aliases for EAF2 genePrevious GC identifers: GC03P121194 GC03P122835 GC03P122874 |
Summaries for EAF2(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1Function: Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivoGene Wiki entry for EAF2 |
Genomic Location for EAF2
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the EAF2 gene 
Entrez Gene cytogenetic band: 3q13.33 Ensembl cytogenetic band: 3q13.33 HGNC cytogenetic band: 3q21.1EAF2 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03P123036:
(about GC identifiers)
Start:
|
123,036,724 bp from pter |
End:
|
123,088,063 bp from pter |
Size:
|
51,340 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000003.10 NT_005612.15
| Proteins for EAF2
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1 (See
protein sequence)Recommended Name: ELL-associated factor 2 Size: 260 amino acids; 28792 Da
Subunit: Isoform 1 and isoform 2 interact with TCEA1 (By similarity). Interacts with ELL and ELL2
Subcellular location: Nucleus speckle. Note=Colocalizes with ELL to nuclear speckles
Secondary accessions: Q9NZ82Alternative splicing: 2 isoforms: Q96CJ1-1 Q96CJ1-2 Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_060926.2
ENSEMBL proteins: ENSP00000273668
Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
3 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for EAF2: Assays for EAF2: | Protein
Domains/ Families for EAF2(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q96CJ1
ProtoNet protein and cluster: Q96CJ1 UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1Similarity: Belongs to the EAF family | Gene Function for EAF2
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018456
Applied Biosystems Silencer® siRNAs for EAF2
Sigma-Aldrich siRNA for EAF2  Sigma-Aldrich shRNA for EAF2  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_018456                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_018456                                  untagged cDNA clone in CMV expression vector: NM_018456 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_018456
UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1Function: Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivoInduction: By androgen6 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Eaf2):
2 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions for EAF2
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
Gene Network CentralTM Interacting Genes and Proteins Network for EAF2 
3 Interacting proteins for EAF2 (ENSP000002736683 Q96CJ11) via UniProtKB, MINT, and/or STRINGAbout this table
3 Gene Ontology (GO) biological process terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006355 | regulation of transcription, DNA-dependent |
IEA | -- | | GO:0006915 | apoptosis |
IEA | -- | | GO:0045941 | positive regulation of transcription |
IEA | -- | About this table
|
Drugs & Compounds for EAF2(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for EAF2
|
Transcripts for EAF2(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018456
Sigma-Aldrich siRNA for EAF2  Sigma-Aldrich shRNA for EAF2  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_018456 REFSEQ mRNAs for EAF2 gene: NM_018456.4
Applied Biosystems TaqMan ® Gene Expression Assays: NM_018456               OriGene GFP tagged cDNA clone in CMV expression vector: NM_018456                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_018456                                  untagged cDNA clone in CMV expression vector: NM_018456  Additional cDNA sequence: AF217516.1 AF517829.1 AK301560.1 AY049020.1 BC014209.2 BC014589.1 5 DOTS entries: DT.409235 DT.100749732 DT.101963803 DT.95234892 DT.92425242 24/89 AceView cDNA sequences (see all 89
):BE676892 AI913493 AY049020 AW615277 AI984339 AI376845 BG686288 BF591218 BU162139 CD365824 BE673409 AF517829 AI431802 BC014209 BE463704 AI339163 AA596045 CA445892 BF476192 AI913739 AI498291 BC014589 BF173204 AW771560
highest scoring ESTs for EAF2:AI676178 AA557315 AA596045 AA808665 AA971970 AF217516 AF517829 AI207014 AI339163 AI357930 Unigene Cluster for EAF2: ELL associated factor 2 Hs.477325 [show with all ESTs]Unigene Representative Sequence: BF965153
GeneLoc Exon Structure
1 Ensembl transcript including schematic representation: ENST00000273668
|
Expression for EAF2
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| EAF2 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for EAF2
1 / 2 / 3 6 probe-sets matching EAF2 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: TATAATTTAT
SOURCE GeneReport for Unigene cluster: Hs.477325 UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1Tissue specificity: Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymphe node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes |
Orthologs for EAF2
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for EAF2 gene from 5/9 species (see all 9
)
About this table Species with no ortholog for EAF2
ENSEMBL Gene Tree for EAF2 | Paralogs for EAF2(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for EAF2 gene
- EAF12
|
SNPs/Variants for EAF2(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for EAF2 (up to first 250kb)
|
Disorders & Mutations for EAF2
(in which this Gene is Involved, According to
OMIM, UniProtKB,
Novoseek, PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 607659
3 Novoseek disease relationships for EAF2 gene
About this table
|
Medical News for EAF2(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for EAF2 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/12 PubMed articles for EAF2 gene (see all 12
):- ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties. (PubMed id 12446457)1, 2, 3, 4 Simone F.... Thirman M.J. (2003)
- Suppression of prostate tumor growth by U19, a novel testosterone-regulated apoptosis inducer. (PubMed id 12907652)1, 2, 3, 4 Xiao W....Wang Z. (2003)
- ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL. (PubMed id 16006523)1, 3, 4 Kong S.E.... Conaway R.C. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. (PubMed id 11042152)3, 4 Zhang Q.H....Chen Z. (2000)
- FB1, an E2A fusion partner in childhood leukemia, interacts with U19/EAF2 and inhibits its transcriptional activity. (PubMed id 17395368)1, 3 Jiang F....Wang Z. (2007)
- A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)4 Dephoure N.... Gygi S.P. (2008)
- ELL binding regulates U19/Eaf2 intracellular localization, stability, and transactivation. (PubMed id 16114057)3 Xiao W....Wang Z. (2006)
- Identification of a novel tissue-specific transcriptional activator FESTA as a protein that interacts with the transcription elongation factor S-II. (PubMed id 12761297)3 Saso K....Sekimizu K. (2003)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)3 Strausberg R.L....Marra M.A. (2002)
|
Search for EAF2
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing EAF2
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing EAF2
(According to HUGE)
About This Section
| -- |
Specialized Databases showing EAF2(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for EAF2(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for EAF2:

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Incremental update: 13 Oct 2009
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