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EAF2 Gene

protein-coding   GIFtS: 50

GC03P123036
ELL associated factor 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
BM040 1, 2
TRAITS 1, 2, 3
U19 1, 2
Descriptions
ELL associated factor 2 2
ELL-associated factor 2 2
Testosterone-regulated apoptosis inducer and tumor
suppressor protein 3
testosterone regulated apoptosis inducer and tumor
suppressor 2
External Ids
HGNC: 231151
Entrez Gene: 558402
UniProtKB: Q96CJ13
Ensembl: ENSG000001450887
Search outside databases for aliases for EAF2 gene

Previous GC identifers: GC03P121194 GC03P122835 GC03P122874

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1
Function: Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity).
Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of
apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo

Gene Wiki entry for EAF2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the EAF2 gene  

Entrez Gene cytogenetic band: 3q13.33   Ensembl cytogenetic band:  3q13.33   HGNC cytogenetic band: 3q21.1

EAF2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P123036:     (about GC identifiers)

Start:
123,036,724 bp from pter
End:
123,088,063 bp from pter
Size:
51,340 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_005612.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1 (See protein sequence)
Recommended Name: ELL-associated factor 2  
Size: 260 amino acids; 28792 Da
Subunit: Isoform 1 and isoform 2 interact with TCEA1 (By similarity). Interacts with ELL and ELL2
Subcellular location: Nucleus speckle. Note=Colocalizes with ELL to nuclear speckles
Secondary accessions: Q9NZ82
Alternative splicing: 2 isoforms:  Q96CJ1-1   Q96CJ1-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_060926.2  

    ENSEMBL proteins: 
    ENSP00000273668 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (EAF2)
    Human Recombinant Proteins from Abnova (EAF2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005654 nucleoplasm IEA--
    GO:0016607 nuclear speck IEA--
    About this table

    Antibodies for EAF2: 
    Browse Antibodies Central at Invitrogen
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    Sigma-Aldrich Antibodies for EAF2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (EAF2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (EAF2)
    Novus Biologicals Antibody for EAF2

    Assays for EAF2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR019194 ELL-associated_factor


       GeneDecks  EAF2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q96CJ1

    ProtoNet protein and cluster: Q96CJ1

    UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1
    Similarity: Belongs to the EAF family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (EAF2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018456

                  Applied Biosystems Silencer® siRNAs for EAF2

                  Sigma-Aldrich siRNA for EAF2  
                         Sigma-Aldrich shRNA for EAF2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for EAF2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018456
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018456
                                     untagged cDNA clone in CMV expression vector: NM_018456 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_018456

    UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1
    Function: Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity).
    Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of
    apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo
    Induction: By androgen

    6 MGI mutant phenotypes (inferred from 1 allele(MGI details for Eaf2):

    endocrine/exocrine glandlife span-post-weaning/agingreproductive systemskin/coat/nails
    tumorigenesis

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IPI17395368
    GO:0016563 transcription activator activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  EAF2 


    3 Interacting proteins for EAF2 (ENSP000002736683 Q96CJ11) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TFPTP0C1Z61STRING (score=.732) EBI-1245604, EBI-1245626
    ELLENSP000002628093STRING (score=.978)
    VHLENSP000002564743STRING (score=.876)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0006915 apoptosis IEA--
    GO:0045941 positive regulation of transcription IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for EAF2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (EAF2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018456

                  Sigma-Aldrich siRNA for EAF2  
                         Sigma-Aldrich shRNA for EAF2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_018456  

    REFSEQ mRNAs for EAF2 gene: 

    NM_018456.4   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_018456  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018456
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018456
                                     untagged cDNA clone in CMV expression vector: NM_018456 

    Additional cDNA sequence: 

    AF217516.1 AF517829.1 AK301560.1 AY049020.1 BC014209.2 BC014589.1 

    5 DOTS entries:

    DT.409235  DT.100749732  DT.101963803  DT.95234892  DT.92425242 

    24/89 AceView cDNA sequences (see all 89 ):

    BE676892 AI913493 AY049020 AW615277 AI984339 AI376845 BG686288 BF591218 
    BU162139 CD365824 BE673409 AF517829 AI431802 BC014209 BE463704 AI339163 
    AA596045 CA445892 BF476192 AI913739 AI498291 BC014589 BF173204 AW771560 

    highest scoring ESTs for EAF2:

    AI676178 AA557315 AA596045 AA808665 AA971970 AF217516 AF517829 AI207014 AI339163 AI357930 

    Unigene Cluster for EAF2:

    ELL associated factor 2
    Hs.477325  [show with all ESTs]
    Unigene Representative Sequence: BF965153


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000273668  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    EAF2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for EAF2

    1 / 2 / 3

    6 probe-sets matching EAF2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    43990_at2, 3 U95-B 1 1.00 1.00 0.92 1.30 AI676178 1.00 1.00 1.00 1

    84074_at2, 3 U95-D 1 0.56 1.00 -0.07 0.73 AI935105 0.60 1.00 0.82 1

    219551_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018456 0.60 1.00 0.82 1

    1568672_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    219551_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1568673_s_at2 U133Plus2 1 0.27 1.00 -- -- -- -- -- -- --
    GeneDecks  EAF2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TATAATTTAT

    SOURCE GeneReport for Unigene cluster: Hs.477325

    UniProtKB/Swiss-Prot: EAF2_HUMAN, Q96CJ1
    Tissue specificity: Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas,
    spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymphe node, spinal gland,
    stomach, thyroid, trachea, thymus, liver and leukocytes

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for EAF2 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    EAF21   -- ELL associated factor 2 88.98(n)
    85.71(a)
    608554  XM_845607.1  XP_850700.1 
    chimpanzee
    (Pan troglodytes)
    EAF21   -- ELL associated factor 2 99.62(n)
    98.85(a)
    745927  XM_001165769.1  XP_001165769.1 
    cow
    (Bos taurus)
    EAF21   -- ELL associated factor 2 89.83(n)
    88.42(a)
    613523  NM_001046507.1  NP_001039972.1 
    rat
    (Rattus norvegicus)
    Eaf21   -- ELL associated factor 2 84.68(n)
    80.69(a)
    266787  NM_172047.1  NP_742044.1 
    mouse
    (Mus musculus)
    Eaf25 16 ELL associated factor 2   -- BG144504 
    About this table        Species with no ortholog for EAF2

    ENSEMBL Gene Tree for EAF2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for EAF2 gene
    EAF12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/271 NCBI SNPs in EAF2 are shown (see all 271 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 85)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs117132681,2
    A,C123088294(+) atttgC/Ttaaaa 1 -- ng310--------
    rs98840181,2
    C,F,H123046045(+) GTTGGT/CGAAGG 1 G/G syn1 ese37Minor allele frequency- C:0.36EU EA WA NA 562
    rs19203211,2
    A,C,F,H,O123039941(+) GATTAT/ATCAGC 1 -- int111Minor allele frequency- A:0.46EU EA WA NA 976
    rs19203221,2
    A,C,F,H,O123040223(+) TCTATG/TTAGTC 1 -- int120Minor allele frequency- T:0.35MN EU EA WA NA 3334
    rs67674091,2
    A,C,F,H123047679(+) TTACCG/ATGGTT 1 -- int112Minor allele frequency- A:0.32EU EA WA NA 1200
    rs98786161,2
    A,C,F,H123084267(+) atatgC/Tgaaag 1 -- int14Minor allele frequency- T:0.06EU EA WA 416
    --
    rs64386811,2
    A,C,F,H123062289(+) tttgaC/Atggtg 1 -- int17Minor allele frequency- A:0.15EU EA WA NA 560
    rs124963181,2
    A,C,F,H123044190(+) GTTTTG/TGTTCA 1 -- int18Minor allele frequency- T:0.08EU EA WA 836
    --
    rs19203231,2
    A,C,F,H123043737(+) ATAATA/TAATAA 1 -- int14Minor allele frequency- T:0.45EU EA WA 412
    rs19202981,2
    A,C,F,H123080578(-) aaaagA/Gcaaaa 1 -- int14Minor allele frequency- G:0.09EU EA WA 418
    About this table

    HapMap Linkage Disequilibrium images for EAF2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607659

    3 Novoseek disease relationships for EAF2 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    allelic loss 73.16 4 17873910 (1), 12907652 (1)
    prostate cancer 39.55 6 12907652 (2), 17873910 (1)
    tumors 2.44 10 12907652 (5), 17873910 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/12 PubMed articles for EAF2 gene (see all 12 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 55840 HGNC: 23115 AceView: EAF2 Ensembl:ENSG00000145088 euGenes: HUgn55840
    ECgene: EAF2 H-InvDB: EAF2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for EAF2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for EAF2 Antibodies for EAF2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for EAF2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Antibodies for EAF2

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