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Aliases & Descriptions for E2F8
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| E2F-8 3 | | FLJ23311 1, 2 |
| | | Descriptions |
|---|
| E2F family member 8 2 | | E2F transcription factor 8 2 |
|
| | Search outside databases for aliases for E2F8 gene |
Summaries for E2F8(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for E2F8: E2F transcription factors, such as E2F8, are essential for orchestrating expression of genes required for cell cycle progression and proliferation (Christensen et al., 2005 [PubMed 16179649]).[supplied by OMIM] UniProtKB/Swiss-Prot: E2F8_HUMAN, A0AVK6Function: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession |
Genomic Location for E2F8
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the E2F8 gene 
Entrez Gene cytogenetic band: 11p15.1 Ensembl cytogenetic band: 11p15.1 HGNC cytogenetic band: 11p15E2F8 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 11 GeneLoc Exon Structure GeneLoc location for GC11M019203:
(about GC identifiers)
Start:
|
19,202,186 bp from pter |
End:
|
19,219,965 bp from pter |
Size:
|
17,780 bases |
Orientation:
|
minus strand |
RefSeq DNA sequence:- NC_000011.8 NT_009237.17
| Proteins for E2F8
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: E2F8_HUMAN, A0AVK6 (See
protein sequence)Recommended Name: Transcription factor E2F8 Size: 867 amino acids; 94166 Da
Subunit: Forms heterodimers with E2F7 and, to a lesser extent, homodimers. Dimerization is important for DNA binding
Subcellular location: Nucleus
Sequence caution: Sequence=AAH28244.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part;
Secondary accessions: Q2VPJ3 Q3C1U6 Q5BKY4 Q8N340 Q9H5M0Post-translational modifications:
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_078956.2
ENSEMBL proteins: ENSP00000250024 ENSP00000379463
Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 
2 Gene Ontology (GO) cellular component terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005634 | nucleus |
IEA | -- | | GO:0005667 | transcription factor complex |
IEA | -- | About this table
Antibodies for E2F8: Assays for E2F8: | Protein
Domains/ Families for E2F8(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry A0AVK6
ProtoNet protein and cluster: A0AVK6 UniProtKB/Swiss-Prot: E2F8_HUMAN, A0AVK6Domain: Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer (By similarity)Similarity: Belongs to the E2F/DP family | Gene Function for E2F8
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|                OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_024680
Applied Biosystems Silencer® siRNAs for E2F8
Sigma-Aldrich siRNA for E2F8  Sigma-Aldrich shRNA for E2F8  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024680                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_024680                                  untagged cDNA clones in CMV expression vector (see all 2): BC028244 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_024680
UniProtKB/Swiss-Prot: E2F8_HUMAN, A0AVK6Function: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession7 MGI mutant phenotypes (inferred from 2 alleles ) (MGI details for E2f8):
1 Gene Ontology (GO) molecular function term (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0003700 | transcription factor activity |
IEA | -- | About this table | Pathways & Interactions for E2F8
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
3 Interacting proteins for E2F8 (ENSP000002500243 A0AVK62) via UniProtKB, MINT, and/or STRINGAbout this table
3 Gene Ontology (GO) biological process terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006350 | transcription |
IEA | -- | | GO:0006355 | regulation of transcription, DNA-dependent |
IEA | -- | | GO:0007049 | cell cycle |
IEA | -- | About this table
|
Drugs & Compounds for E2F8(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for E2F8
|
Transcripts for E2F8(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|                OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_024680
Sigma-Aldrich siRNA for E2F8  Sigma-Aldrich shRNA for E2F8  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_024680 REFSEQ mRNAs for E2F8 gene: NM_024680.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_024680               OriGene GFP tagged cDNA clone in CMV expression vector: NM_024680                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_024680                                  untagged cDNA clones in CMV expression vector (see all 2): BC028244  Additional cDNA sequence: AB231781.1 AB231782.1 AB231783.1 AK026964.1 AK055206.1 AK225338.1 AK292688.1 BC028244.1 BC090877.1 BC108700.1 BC126400.1 BC126402.1 CR605237.1 6 DOTS entries: DT.309870 DT.101983392 DT.95359923 DT.120737908 DT.100772254 DT.75148880 24/37 AceView cDNA sequences (see all 37
):NM_024680 CR605237 CF593498 CB959621 BF055385 CD251827 BC028244 AI351690 BU164108 BG165330 BM972658 AW051975 BG698178 AA693962 BX363885 AA430401 AL597376 BX474754 AW268843 BX504614 AK055206 AI005104 AK026964 BI858526
highest scoring ESTs for E2F8:AI005104 AL597376 BQ214482 BX504614 CB959621 CD109365 AA453131 AA476328 AA693962 AA746407 Unigene Cluster for E2F8: E2F transcription factor 8 Hs.523526 [show with all ESTs]Unigene Representative Sequence: NM_024680
GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for E2F8
| ExUns: | 1a | · | 1b | · | 1c | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | ^ | 5 | ^ | 6a | · | 6b | ^ | 7a | · | 7b | ^ | 8a | · | 8b | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12a | · | 12b | ^ | 13 | ^ | 14 | |
| SP1: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | - |   | - |   | - |   | - |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for E2F8
2 Ensembl transcripts including schematic representations: ENST00000250024
ENST00000396159
|
Expression for E2F8
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| E2F8 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for E2F8
1 / 2 / 3 3 probe-sets matching E2F8 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: GATTTTGAGG
SOURCE GeneReport for Unigene cluster: Hs.523526
|
Orthologs for E2F8
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for E2F8 gene from 5/9 species (see all 9
)
About this table Species with no ortholog for E2F8
ENSEMBL Gene Tree for E2F8 | Paralogs for E2F8(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for E2F8 gene
- E2F72
|
SNPs/Variants for E2F8(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for E2F8 (up to first 250kb)
|
Disorders & Mutations for E2F8
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 612047
|
Medical News for E2F8(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for E2F8 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/12 PubMed articles for E2F8 gene (see all 12
):- Characterization of E2F8, a novel E2F-like cell-cycle regulated repressor of E2F-activated transcription. (PubMed id 16179649)3, 4 Christensen J.... Helin K. (2005)
- Cloning and characterization of mouse E2F8, a novel mammalian E2F family member capable of blocking cellular proliferation. (PubMed id 15722552)2, 3 Maiti B....Leone G. (2005)
- E2F-8: an E2F family member with a similar organization of DNA- binding domains to E2F-7. (PubMed id 15897886)3, 4 Logan N....La Thangue N.B. (2005)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)3, 4 Ota T....Sugano S. (2004)
- Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. (PubMed id 18220336)4 Cantin G.T....Yates J.R. III (2008)
- DNA-damage response control of E2F7 and E2F8. (PubMed id 18202719)3 Zalmas L.P....La Thangue N.B. (2008)
- Synergistic function of E2F7 and E2F8 is essential for cell survival and embryonic development. (PubMed id 18194653)4 Li J.... de Bruin A. (2008)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)4 Gerhard D.S....Malek J. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)3 Strausberg R.L....Marra M.A. (2002)
- Construction and characterization of a full length-enriched and a 5'- end-enriched cDNA library. (PubMed id 9373149)3 Suzuki Y.... Sugano S. (1997)
|
Search for E2F8
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing E2F8
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing E2F8
(According to HUGE)
About This Section
| -- |
Specialized Databases showing E2F8(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for E2F8(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for E2F8:

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| | Recombinant Proteins (E2F8) | | Antibodies (E2F8) |
| | | Search Tocris compounds for E2F8 |
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Incremental update: 13 Oct 2009
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