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E2F8 Gene

protein-coding   GIFtS: 53

GC11M019203
E2F transcription factor 8
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
E2F-8 3
FLJ23311 1, 2
Descriptions
E2F family member 8 2
E2F transcription factor 8 2
External Ids
HGNC: 247271
Entrez Gene: 797332
UniProtKB: A0AVK63
Ensembl: ENSG000001291737
Search outside databases for aliases for E2F8 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for E2F8:
E2F transcription factors, such as E2F8, are essential for orchestrating expression of genes
required for cell cycle progression and proliferation (Christensen et al., 2005 [PubMed
16179649]).[supplied by OMIM]

UniProtKB/Swiss-Prot: E2F8_HUMAN, A0AVK6
Function: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP
proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1
transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for
normal cell cycle progession

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the E2F8 gene  

Entrez Gene cytogenetic band: 11p15.1   Ensembl cytogenetic band:  11p15.1   HGNC cytogenetic band: 11p15

E2F8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M019203:     (about GC identifiers)

Start:
19,202,186 bp from pter
End:
19,219,965 bp from pter
Size:
17,780 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000011.8  NT_009237.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: E2F8_HUMAN, A0AVK6 (See protein sequence)
Recommended Name: Transcription factor E2F8  
Size: 867 amino acids; 94166 Da
Subunit: Forms heterodimers with E2F7 and, to a lesser extent, homodimers. Dimerization is
important for DNA binding
Subcellular location: Nucleus
Sequence caution: Sequence=AAH28244.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence.
Sequence of unknown origin in the N-terminal part;
Secondary accessions: Q2VPJ3 Q3C1U6 Q5BKY4 Q8N340 Q9H5M0

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_078956.2  

    ENSEMBL proteins: 
    ENSP00000250024 ENSP00000379463 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (E2F8)
    Human Recombinant Proteins from Abnova (E2F8)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005667 transcription factor complex IEA--
    About this table

    Antibodies for E2F8: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for E2F8
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (E2F8), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (E2F8)
    Novus Biologicals Antibody for E2F8

    Assays for E2F8: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR003316 E2F_TDP
     IPR011991 Wing_hlx_DNA_bd
     IPR015633 E2F


       GeneDecks  E2F8 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry A0AVK6

    ProtoNet protein and cluster: A0AVK6

    UniProtKB/Swiss-Prot: E2F8_HUMAN, A0AVK6
    Domain: Both DNA-binding domains are required for DNA-binding and are proposed to form an
    intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer
    (By similarity)
    Similarity: Belongs to the E2F/DP family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (E2F8)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (E2F8)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_024680

                  Applied Biosystems Silencer® siRNAs for E2F8

                  Sigma-Aldrich siRNA for E2F8  
                         Sigma-Aldrich shRNA for E2F8  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for E2F8
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_024680
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_024680
                                     untagged cDNA clones in CMV expression vector (see all 2): BC028244 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_024680

    UniProtKB/Swiss-Prot: E2F8_HUMAN, A0AVK6
    Function: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP
    proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1
    transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for
    normal cell cycle progession

    7 MGI mutant phenotypes (inferred from 2 alleles(MGI details for E2f8):

    cellularembryogenesisgrowth/sizelethality-prenatal/perinatal
    life span-post-weaning/agingnormal

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700 transcription factor activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    3 Interacting proteins for E2F8 (ENSP000002500243 A0AVK62) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    E2F7Q96AV82MINT-6541638 MINT-6541689 MINT-6541625 MINT-6541666
    CDC6ENSP000002097283STRING (score=.774)
    --ENSP000003598673STRING (score=.873)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0007049 cell cycle IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for E2F8

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (E2F8)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (E2F8)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_024680

                  Sigma-Aldrich siRNA for E2F8  
                         Sigma-Aldrich shRNA for E2F8  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_024680  

    REFSEQ mRNAs for E2F8 gene: 

    NM_024680.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_024680  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_024680
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_024680
                                     untagged cDNA clones in CMV expression vector (see all 2): BC028244 

    Additional cDNA sequence: 

    AB231781.1 AB231782.1 AB231783.1 AK026964.1 AK055206.1 AK225338.1 AK292688.1 BC028244.1 
    BC090877.1 BC108700.1 BC126400.1 BC126402.1 CR605237.1 

    6 DOTS entries:

    DT.309870  DT.101983392  DT.95359923  DT.120737908  DT.100772254  DT.75148880 

    24/37 AceView cDNA sequences (see all 37 ):

    NM_024680 CR605237 CF593498 CB959621 BF055385 CD251827 BC028244 AI351690 
    BU164108 BG165330 BM972658 AW051975 BG698178 AA693962 BX363885 AA430401 
    AL597376 BX474754 AW268843 BX504614 AK055206 AI005104 AK026964 BI858526 

    highest scoring ESTs for E2F8:

    AI005104 AL597376 BQ214482 BX504614 CB959621 CD109365 AA453131 AA476328 AA693962 AA746407 

    Unigene Cluster for E2F8:

    E2F transcription factor 8
    Hs.523526  [show with all ESTs]
    Unigene Representative Sequence: NM_024680


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for E2F8

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14
    SP1:                                                                                -     -     -     -     -                     
    SP2:                                                                                                                              
    SP3:              -     -                                                                                                         
    SP4:                    -                                                                                                         

    About this scheme

    ECgene alternative splicing isoforms for E2F8

    2 Ensembl transcripts including schematic representations:
    ENST00000250024  ENST00000396159  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    E2F8 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for E2F8

    1 / 2 / 3

    3 probe-sets matching E2F8 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    57790_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AI005104 0.80 1.00 0.91 1

    219990_at2, 3 U133-A 2 1.00 0.95 -- -- NM_024680 0.60 1.00 0.82 1

    219990_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
    GeneDecks  E2F8 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GATTTTGAGG

    SOURCE GeneReport for Unigene cluster: Hs.523526
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for E2F8 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    E2F81   -- E2F transcription factor 8 90.01(n)
    89.93(a)
    476885  XM_534087.2  XP_534087.2 
    chimpanzee
    (Pan troglodytes)
    E2F81   -- E2F transcription factor 8 99.35(n)
    98.96(a)
    451072  XM_001173623.1  XP_001173623.1 
    cow
    (Bos taurus)
    E2F81   -- E2F transcription factor 8 88.44(n)
    84.69(a)
    786629  XM_001254260.1  XP_001254261.1 
    rat
    (Rattus norvegicus)
    E2f81   -- E2F transcription factor 8 84.1(n)
    83.36(a)
    308607  XM_218601.4  XP_218601.4 
    mouse
    (Mus musculus)
    E2f81, 5 75
    E2F transcription factor 81, 5 82.75(n)1
    84.19(a)1
    1089611  NM_001013368.41  NP_001013386.21 
     AA4100485  AI2562885  (see all 19)
    About this table        Species with no ortholog for E2F8

    ENSEMBL Gene Tree for E2F8
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for E2F8 gene
    E2F72  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/91 NCBI SNPs in E2F8 are shown (see all 91 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 67)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs22920611,2
    A,C,F,H19202180(-) GTTAGC/TATTTT 1 -- ng519Minor allele frequency- T:0.35EA EU WA 2302
    rs7932781,2
    A,C,F,H19202430(-) AGCAAG/ATATGC 1 -- ut317Minor allele frequency- A:0.30MN EU WA EA NA 2002
    rs7932831,2
    A,C,F,H19221073(-) TATTTC/TAAATC 1 -- ng314Minor allele frequency- T:0.20EU EA WA 418
    rs7932741,2
    C,F,H19203861(-) CCCCAA/GTTGCA 1 I/V mis111Minor allele frequency- T:0.00MN EU EA WA NA 1086
    --
    rs7932771,2
    A,C19202620(-) ATATAC/TACACA 1 -- ut310--------
    rs37409661,2
    C,F,H19208839(+) AGAGAT/AGGGTG 1 P/P syn1 ese38Minor allele frequency- A:0.01EA NA EU WA 2054
    rs118210231,2
    C,F,H19208402(+) GCTGCG/AAGCTG 1 L/L syn1 ese34Minor allele frequency- A:0.13EU EA WA 420
    rs7969691,2
    C,F,H19203985(-) TAGACC/TTTGTT 1 T/T syn15Minor allele frequency- T:0.02EU EA WA NA 480
    rs37409651,2
    F,H19203674(+) TTCAAC/TGGTGT 1 P/P syn1 ese35Minor allele frequency- T:0.01EA EU WA 1920
    rs122730011,2
    F,H19207865(+) GGTGGC/GGTCAC 1 T/T syn14Minor allele frequency- G:0.14EU EA WA 390
    About this table

    HapMap Linkage Disequilibrium images for E2F8 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 612047

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/12 PubMed articles for E2F8 gene (see all 12 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 79733 HGNC: 24727 AceView: FLJ23311 Ensembl:ENSG00000129173 euGenes: HUgn79733
    ECgene: E2F8 H-InvDB: E2F8
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for E2F8:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for E2F8 Antibodies for E2F8
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
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