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E2F7 Gene

protein-coding   GIFtS: 53

GC12M075917
E2F transcription factor 7
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
E2F-7 3
FLJ12981 2
Descriptions
E2F transcription factor 7 2
External Ids
HGNC: 238201
Entrez Gene: 1444552
UniProtKB: Q96AV83
Ensembl: ENSG000001658917
Search outside databases for aliases for E2F7 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for E2F7:
E2F transcription factors, such as E2F7, play an essential role in the regulation of cell cycle
progression (Di Stefano et al., 2003 [PubMed 14633988]).[supplied by OMIM]

UniProtKB/Swiss-Prot: E2F7_HUMAN, Q96AV8
Function: Along with E2F8, inhibitor of E2F-dependent transcription that is important for the
control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription (By similarity).
Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears
to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle
progession

Gene Wiki entry for E2F7

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the E2F7 gene  

Entrez Gene cytogenetic band: 12q21.2   Ensembl cytogenetic band:  12q21.2   HGNC cytogenetic band: 12q21.1

E2F7 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M075917:     (about GC identifiers)

Start:
75,939,157 bp from pter
End:
75,983,491 bp from pter
Size:
44,335 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000012.10  NT_019546.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: E2F7_HUMAN, Q96AV8 (See protein sequence)
Recommended Name: Transcription factor E2F7  
Size: 911 amino acids; 99845 Da
Subunit: Forms homodimers and, to a lesser extent, heterodimers with E2F8. Dimerization is
important for DNA binding
Subcellular location: Nucleus
Secondary accessions: A6NC74 B2RMR7
Alternative splicing: 2 isoforms:  Q96AV8-1   Q96AV8-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_976328.2  

    ENSEMBL proteins: 
    ENSP00000343225 ENSP00000323246 


    Human Recombinant Proteins 
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    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (E2F7)
    Human Recombinant Proteins from Abnova (E2F7)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005667 transcription factor complex IEA--
    About this table

    Antibodies for E2F7: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (E2F7), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for E2F7

    Assays for E2F7: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR003316 E2F_TDP
     IPR011991 Wing_hlx_DNA_bd
     IPR015633 E2F


       GeneDecks  E2F7 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q96AV8

    ProtoNet protein and cluster: Q96AV8

    UniProtKB/Swiss-Prot: E2F7_HUMAN, Q96AV8
    Domain: Both DNA-binding domains are required for DNA-binding and are proposed to form an
    intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer
    Similarity: Belongs to the E2F/DP family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (E2F7)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (E2F7)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_203394

                  Applied Biosystems Silencer® siRNAs for E2F7

                  Sigma-Aldrich siRNA for E2F7  
                         Sigma-Aldrich shRNA for E2F7  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_203394
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_203394
                                     untagged cDNA clone in CMV expression vector: NM_203394 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_203394

    UniProtKB/Swiss-Prot: E2F7_HUMAN, Q96AV8
    Function: Along with E2F8, inhibitor of E2F-dependent transcription that is important for the
    control of the E2F1-TP53 apoptotic pathway. Directly represses E2F1 transcription (By similarity).
    Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Appears
    to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle
    progession

    7 MGI mutant phenotypes (inferred from 2 alleles(MGI details for E2f7):

    cellularembryogenesisgrowth/sizelethality-prenatal/perinatal
    life span-post-weaning/agingnormal

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700 transcription factor activity IEA--
    GO:0016564 transcription repressor activity IEA--
    GO:0042802 identical protein binding IPI15133492
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/6 Interacting proteins for E2F7 (Q96AV81, 2 ENSP000003232463) via UniProtKB, MINT, and/or STRING (see all 6 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    E2F7Q96AV81EBI-1386765, EBI-1386765
    RUVBL1Q9Y2651EBI-1386765, EBI-353675
    E2F8A0AVK62MINT-6541638 MINT-6541689 MINT-6541625 MINT-6541666
    CDC6ENSP000002097283STRING (score=.849)
    STUB1ENSP000002195483STRING (score=.701)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0007049 cell cycle IEA--
    GO:0008285 negative regulation of cell proliferation IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for E2F7

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (E2F7)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (E2F7)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_203394

                  Sigma-Aldrich siRNA for E2F7  
                         Sigma-Aldrich shRNA for E2F7  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_203394  

    REFSEQ mRNAs for E2F7 gene: 

    NM_203394.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_203394  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_203394
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_203394
                                     untagged cDNA clone in CMV expression vector: NM_203394 

    Additional cDNA sequence: 

    AK023043.1 AK096316.1 AK097677.1 BC016658.1 BC017481.1 BC136366.1 BC136367.1 CR606651.1 

    4 DOTS entries:

    DT.91850328  DT.100747226  DT.121145825  DT.99973123 

    24/56 AceView cDNA sequences (see all 56 ):

    AK023043 BU621552 NM_203394 CR606651 BE221067 BI826210 AW467385 AI886283 
    BC016658 AI911719 BC017481 BG258228 BG033282 BU176322 AK096316 AW294416 
    AA905212 AW151733 AA133520 BX370359 BF173473 AA133617 AA918458 BI463160 

    highest scoring ESTs for E2F7:

    AA918458 AI341146 BC017481 BE279439 BG774607 BX283562 AA133617 AA604782 AA664632 AA747101 

    Unigene Cluster for E2F7:

    E2F transcription factor 7
    Hs.416375  [show with all ESTs]
    Unigene Representative Sequence: NM_203394


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for E2F7

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b · 10c ^ 11a · 11b ^ 12 ^ 13 ^ 14a · 14b
    SP1:                                                                                                                                    
    SP2:              -                                                                                                                     
    SP3:                                                                                                                                    
    SP4:                                                                                                                                    
    SP5:                                                                                                              -     -               

    About this scheme

    ECgene alternative splicing isoforms for E2F7

    2 Ensembl transcripts including schematic representations:
    ENST00000339887  ENST00000322886  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    E2F7 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for E2F7

    1 / 2 / 3

    6 probe-sets matching E2F7 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    82650_at2, 3 U95-E 1 1.00 1.00 0.94 1.01 AI341146 0.80 1.00 0.91 1
    72533_at2, 3 U95-D 1 1.00 1.00 0.66 1.01 AA918458 0.80 1.00 0.91 1

    241725_at2, 3 U133-B 1 1.00 1.00 -- -- AW511293 0.60 1.00 0.82 1

    228033_at2, 3 U133-B 1 1.00 1.00 -- -- AI341146 0.80 1.00 0.91 1

    241725_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    228033_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  E2F7 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TAAACTGATT

    SOURCE GeneReport for Unigene cluster: Hs.416375
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for E2F7 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    E2F71   -- E2F transcription factor 7 83.82(n)
    82.89(a)
    482575  XM_539692.2  XP_539692.2 
    chimpanzee
    (Pan troglodytes)
    E2F71   -- E2F transcription factor 7 99.6(n)
    99.56(a)
    452090  XM_001161947.1  XP_001161947.1 
    cow
    (Bos taurus)
    E2F71   -- E2F transcription factor 7 78.18(n)
    75.16(a)
    526129  XM_604488.3  XP_604488.3 
    rat
    (Rattus norvegicus)
    E2f71   -- E2F transcription factor 7 81.39(n)
    80.36(a)
    314818  XM_235118.4  XP_235118.4 
    mouse
    (Mus musculus)
    E2f71, 5 10 (59.00 cM)5
    E2F transcription factor 71, 5 80.75(n)1
    80.04(a)1
    526791  NM_178609.41  NP_848724.21 
     AI6269645  AK0310785  (see all 15)
    About this table        Species with no ortholog for E2F7

    ENSEMBL Gene Tree for E2F7
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for E2F7 gene
    E2F82  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/237 NCBI SNPs in E2F7 are shown (see all 237 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 128)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs3108311,2
    A,C,F,H,O75943472(+) ACCTGA/TTGTAA 1 H/Q mis1 ese316Minor allele frequency- T:0.06NS NA EU EA WA 1080
    rs3107911,2
    A,C,F,H,O75973921(+) AACAAG/ACTTAA 1 L/F mis112Minor allele frequency- A:0.13NA EU EA WA 1024
    --
    rs111168721,2
    A,C,F,H75984260(+) CTAGCC/ATTCTG 1 -- ng313Minor allele frequency- A:0.25EU EA 300
    rs3108551,2
    A,C,F,H75985330(+) AAAGTG/ATGCAA 1 -- ng31 tfbs314Minor allele frequency- A:0.27EU EA WA NS 1042
    rs79583771,2
    A,C,F,H75940098(+) ATATAC/TCCTGC 1 -- ut319Minor allele frequency- T:0.06NA EU EA WA 744
    rs22795761,2
    A,C,F75941640(+) CGGTAG/TCCTAT 1 -- ut31 ese33Minor allele frequency- T:0.24NA 142
    rs111167181,2
    C,F,H75941836(+) GGACCC/TGTGCT 1 -- ut31 ese34Minor allele frequency- T:0.02EU EA WA 404
    rs22795751,2
    C,F,H75941475(+) TGAAGA/GTGGCT 1 -- ut319Minor allele frequency- N:0.00EA EU WA NA 1754
    rs38292951,2
    C,F,H75947750(+) GGGCAT/CGACAA 1 V/M mis112Minor allele frequency- C:0.01EA NA EU WA 2474
    --
    rs3108521,2
    A,C75983718(+) TGCTGA/GGGACG 1 -- ng310--------
    About this table

    HapMap Linkage Disequilibrium images for E2F7 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 612046

    Human Genome Epidemiology Navigator: E2F7 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for E2F7 gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 144455 HGNC: 23820 AceView: E2F7 Ensembl:ENSG00000165891 euGenes: HUgn144455
    ECgene: E2F7 H-InvDB: E2F7
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for E2F7:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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