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E2F4 Gene

protein-coding   GIFtS: 69
GCID: GC16P067226

E2F Transcription Factor 4, P107/P130-Binding

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
E2F Transcription Factor 4, P107/P130-Binding1 2
E2F-42 3
P107/P130-Binding Protein2
Transcription Factor E2F42

External Ids:    HGNC: 31181   Entrez Gene: 18742   Ensembl: ENSG000002052507   OMIM: 6006595   UniProtKB: Q162543   

Export aliases for E2F4 gene to outside databases

Previous GC identifers: GC16P057920 GC16P067603 GC16P066962 GC16P067002 GC16P065783 GC16P053100


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for E2F4 Gene:
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a
crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the
transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved
domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain
which determines interaction with the differentiation regulated transcription factor proteins (DP), a
transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is
embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB,
p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of
proliferation-associated genes, and its gene mutation and increased expression may be associated with human
cancer. (provided by RefSeq, Jul 2008)

GeneCards Summary for E2F4 Gene:
E2F4 (E2F transcription factor 4, p107/p130-binding) is a protein-coding gene. Diseases associated with E2F4 include cervical intraepithelial neoplasia, and retinoblastoma. GO annotations related to this gene include sequence-specific DNA binding transcription factor activity and protein domain specific binding. An important paralog of this gene is E2F1.

UniProtKB/Swiss-Prot: E2F4_HUMAN, Q16254
Function: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site,
5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle
regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from
G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1

Gene Wiki entry for E2F4 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000016.10  NC_018927.2  NT_010498.16  
Regulatory elements:
   Regulatory transcription factor binding sites in the E2F4 gene promoter:
         p53   AP-1   NRSF form 1   MyoD   NRSF form 2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidE2F4 promoter sequence
   Search Chromatin IP Primers for E2F4

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat E2F4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q21-q22   Ensembl cytogenetic band:  16q22.1   HGNC cytogenetic band: 16q21-q22

E2F4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
E2F4 gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16P067226:  view genomic region     (about GC identifiers)

Start:
67,226,068 bp from pter      End:
67,232,821 bp from pter
Size:
6,754 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: E2F4_HUMAN, Q16254 (See protein sequence)
Recommended Name: Transcription factor E2F4  
Size: 413 amino acids; 43960 Da
Subunit: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F
sites. The E2F4/TFDP1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F
transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with
TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription
activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of
E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in
quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52
and LIN54 form a subcomplex that binds to MYBL2. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3,
isoform PML-4 and isoform PML-5)
Developmental stage: Present in the growth-arrested state, its abundance does not change significantly as cells
move into and through the cell cycle
1 PDB 3D structure from and Proteopedia for E2F4:
1CF7 (3D)    
Secondary accessions: A6NGR8 B5BU56 Q12991 Q15328

Explore the universe of human proteins at neXtProt for E2F4: NX_Q16254

Explore proteomics data for E2F4 at MOPED

Post-translational modifications: 

  • Differentially phosphorylated in vivo1
  • Ubiquitination2 at Lys28
  • Modification sites at PhosphoSitePlus

  • See E2F4 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_001941.2  
    ENSEMBL proteins: 
     ENSP00000368686   ENSP00000454477   ENSP00000458082   ENSP00000457239   ENSP00000458706  
    Reactome Protein details: Q16254

    E2F4 Human Recombinant Protein Products:

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    OriGene Protein Over-expression Lysate for E2F4
    OriGene Custom MassSpec
    OriGene Custom Protein Services for E2F4
    GenScript Custom Purified and Recombinant Proteins Services for E2F4
    Novus Biologicals E2F4 Protein
    Novus Biologicals E2F4 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for E2F4

    E2F4 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of E2F4
    R&D Systems Antibodies for E2F4 (E2F-4)
    OriGene Antibodies for E2F4
    OriGene Custom Antibody Services for E2F4
    Novus Biologicals E2F4 Antibodies
    Abcam antibodies for E2F4
    Cloud-Clone Corp. Antibodies for E2F4
    ThermoFisher Antibody for E2F4
    LSBio Antibodies in human, mouse, rat for E2F4

    E2F4 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for E2F4
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for E2F4
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for E2F4
    Cloud-Clone Corp. CLIAs for E2F4


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    4 InterPro protein domains:
     IPR003316 E2F_TDP
     IPR011991 WHTH_DNA-bd_dom
     IPR028312 E2F4
     IPR015633 E2F

    Graphical View of Domain Structure for InterPro Entry Q16254

    ProtoNet protein and cluster: Q16254

    1 Blocks protein domain: IPB003316 Transcription factor E2F/dimerisation partner (TDP)

    UniProtKB/Swiss-Prot: E2F4_HUMAN, Q16254
    Similarity: Belongs to the E2F/DP family


    E2F4 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: E2F4_HUMAN, Q16254
    Function: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site,
    5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle
    regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from
    G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1

         Genatlas biochemistry entry for E2F4:
    transcription factor E2F4,involved in cell proliferation,controlling genes regulating S phase entry and DNA
    synthesis

         Gene Ontology (GO): 5 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IMP11418595
    GO:0003700sequence-specific DNA binding transcription factor activity IDA11418595
    GO:0005515protein binding IPI11418595
    GO:0008134transcription factor binding IPI16360038
    GO:0019904protein domain specific binding IPI16360038
         
    E2F4 for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for E2F4:
     Increased cell death HMECs cel 

         Selected MGI mutant phenotypes (inferred from 2 alleles(MGI details for E2f4) (see all 19):
     behavior/neurological  cardiovascular system  cellular  craniofacial  digestive/alimentary 
     embryogenesis  endocrine/exocrine gland  growth/size/body  hearing/vestibular/ear  hematopoietic system 
     homeostasis/metabolism  immune system  integument  mortality/aging  reproductive system 

    E2F4 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for E2F4: E2f4tm1Lees E2f4tm1Jrn

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for E2F4
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for E2F4

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for E2F4
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for E2F4

    Transcription Factor Targeting: 
    Selected GeneGlobe predicted Target genes for E2F4 (see all 905):
    A4GNT,  AACS,  ABCF2,  ABCF3,  ABHD11,  ABI2,  ABTB1,  ACOT7,  ADAMTS12,  ADAMTS19

    Targeting motifs: HOMER Transcription Factor Regulatory Elements motif viewer 
                                          Consensus sequence:  GGCGGGAAAH 

    miRNA
    Products:
        
    miRTarBase miRNAs that target E2F4:
    hsa-mir-23b-3p (MIRT046375), hsa-mir-615-3p (MIRT039886), hsa-mir-124-3p (MIRT022270), hsa-mir-197-3p (MIRT048137), hsa-mir-98-5p (MIRT048712)

    Block miRNA regulation of human, mouse, rat E2F4 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate E2F4 (see all 14):
    hsa-miR-3163 hsa-miR-3607-3p hsa-miR-576-5p hsa-miR-3614-3p hsa-miR-622 hsa-miR-4263 hsa-miR-1324 hsa-miR-216b
    SwitchGear 3'UTR luciferase reporter plasmidE2F4 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for E2F4
    Predesigned siRNA for gene silencing in human, mouse, rat E2F4

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for E2F4

    Clone
    Products:
         
    OriGene clones in human, mouse for E2F4 (see all 6)
    OriGene ORF clones in mouse, rat for E2F4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: E2F4 (NM_001950)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for E2F4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat E2F4

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for E2F4
    Browse ESI BIO Cell Lines and PureStem Progenitors for E2F4 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for E2F4


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    E2F4_HUMAN, Q16254: Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005667transcription factor complex IEA--
    GO:0005737cytoplasm IDA--

    E2F4 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for E2F4 About   (see all 29)                                                                                              See pathways by source

    SuperPathSelected contained pathways About (see all per SuperPath)
    1Cyclins and Cell Cycle Regulation
    Cyclins and Cell Cycle Regulation0.67
    G1 Phase0.31
    G1-S Phase Transition0.67
    Cyclin D associated events in G10.31
    Cell Cycle Control by BTG Proteins0.36
    2Cell cycle
    Cell cycle0.59
    Cell cycle0.59
    3E2F mediated regulation of DNA replication
    Mitotic G1-G1/S phases0.81
    G1 to S cell cycle control0.35
    4Loss of Function of SMAD2/3 in Cancer
    Transcriptional activity of SMAD2/SMAD3-SMAD4 heterotrimer0.72
    Signaling by TGF-beta Receptor Complex0.60
    SMAD2/SMAD3-SMAD4 heterotrimer regulates transcription0.72
    TGFBR1 KD Mutants in Cancer0.60
    Loss of Function of SMAD2/3 in Cancer0.60
    SMAD2/3 MH2 Domain Mutants in Cancer0.60
    Loss of Function of SMAD4 in Cancer0.60
    SMAD2/3 Phosphorylation Motif Mutants in Cancer0.60
    5Cell cycle Regulation of G1 S transition part 2
    Cell cycle Regulation of G1 S transition part 2 0.38
    Cell cycle ESR1 regulation of G1 S transition0.33
    Cell cycle Cell cycle generic schema 0.38

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 R&D Systems Pathway for E2F4
        Akt Signaling Pathway

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for E2F4 (see all 16)
        Cell Cycle Control by BTG Proteins
    BRCA1 Pathway
    Molecular Mechanisms of Cancer
    Glioblastoma Multiforme
    MNAR-PELP1 and Estrogen Receptor Interaction

    4 GeneGo (Thomson Reuters) Pathways for E2F4
        Cell cycle Regulation of G1/S transition (part 2)
    Cell cycle ESR1 regulation of G1/S transition
    Cell cycle Transition and termination of DNA replication
    Cell cycle Cell cycle (generic schema)

    Selected BioSystems Pathways for E2F4 (see all 7)
        Cell cycle
    Adipogenesis
    G1 to S cell cycle control
    TGF-beta Receptor Signaling Pathway
    Integrated Pancreatic Cancer Pathway

    3 Reactome Pathways for E2F4
        G0 and Early G1
    Cyclin D associated events in G1
    SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription


    2 Kegg Pathways  (Kegg details for E2F4):
        Cell cycle
    TGF-beta signaling pathway


    E2F4 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including E2F4: 
              Cell Cycle in human mouse rat
              TGFB Signaling Targets in human mouse rat
              Cancer PathwayFinder in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for E2F4

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for E2F4 (Q162541, 2, 3 ENSP000003686864) via UniProtKB, MINT, STRING, and/or I2D (see all 132)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    LIN9Q5TKA11, 2, 3, ENSP000003291024EBI-448943,EBI-1389424 MINT-4054190 I2D: score=3 STRING: ENSP00000329102
    RBL2Q089992, 3, ENSP000002621334MINT-8309355 MINT-8309367 MINT-8309378 I2D: score=5 STRING: ENSP00000262133
    DHX30Q7L2E32, 3, ENSP000003926014MINT-4303945 I2D: score=1 STRING: ENSP00000392601
    NR2E3Q9Y5X42, 3MINT-4303929 I2D: score=1 
    ENSG00000206489Q96QC03I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 15):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000083regulation of transcription involved in G1/S transition of mitotic cell cycle IEA--
    GO:0000278mitotic cell cycle TAS--
    GO:0002064epithelial cell development IEA--
    GO:0006351transcription, DNA-templated TAS--
    GO:0006355regulation of transcription, DNA-templated ----

    E2F4 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for E2F4

    6 Novoseek inferred chemical compound relationships for E2F4 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    flavopiridol 46.3 3 15611646 (3)
    roscovitine 42.7 3 15611646 (3)
    serine 15.8 3 9824204 (1), 11759817 (1), 15040009 (1)
    threonine 14 1 15040009 (1)
    benzo(a)pyrene 12 5 16944783 (3), 16600106 (1)
    ascorbic acid 0 3 16944783 (3)



    E2F4 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for E2F4 gene: 
    NM_001950.3  

    Unigene Cluster for E2F4:

    E2F transcription factor 4, p107/p130-binding
    Hs.108371  [show with all ESTs]
    Unigene Representative Sequence: NM_001950
    13 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000379378(uc002erz.3) ENST00000568485 ENST00000567007 ENST00000565849
    ENST00000568839 ENST00000569573 ENST00000563238 ENST00000561904 ENST00000564718
    ENST00000566368 ENST00000568693 ENST00000565226 ENST00000567228
    Congresses - knowledge worth sharing:
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
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    Block miRNA regulation of human, mouse, rat E2F4 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate E2F4 (see all 14):
    hsa-miR-3163 hsa-miR-3607-3p hsa-miR-576-5p hsa-miR-3614-3p hsa-miR-622 hsa-miR-4263 hsa-miR-1324 hsa-miR-216b
    SwitchGear 3'UTR luciferase reporter plasmidE2F4 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for E2F4
    Predesigned siRNA for gene silencing in human, mouse, rat E2F4
    Clone
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    OriGene clones in human, mouse for E2F4 (see all 6)
    OriGene ORF clones in mouse, rat for E2F4
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: E2F4 (NM_001950)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for E2F4
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat E2F4
    Primer
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    OriGene qPCR primer pairs and template standards for E2F4
    OriGene qSTAR qPCR primer pairs in human, mouse for E2F4
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat E2F4
      QuantiTect SYBR Green Assays in human, mouse, rat E2F4
      QuantiFast Probe-based Assays in human, mouse, rat E2F4

    Additional mRNA sequence: 

    AB451292.1 AB451425.1 BC021050.1 BC033180.1 S75174.1 U15641.1 X86096.1 

    15 DOTS entries:

    DT.454769  DT.91753352  DT.100793333  DT.100793325  DT.91990113  DT.100764714  DT.120684194  DT.87006638 
    DT.95155691  DT.91753350  DT.97847998  DT.100793327  DT.100793336  DT.91753348  DT.120684199 

    Selected AceView cDNA sequences (see all 304):

    BF795396 BX332278 BX111165 CN482356 BM665492 AI475130 BQ067481 BM973206 
    CR609179 BM068744 BQ932976 BM739597 BI820657 AA324073 BM547802 BQ421548 
    BI261063 BI908192 BQ574745 AA766116 Z41328 BQ684920 NM_001950 BI906524 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    E2F4 expression in normal human tissues (normalized intensities)      E2F4 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CCTAAGGCTA
    E2F4 Expression
    About this image


    E2F4 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 6) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Neural Tube (Nervous System)    fully expand to see all 3 entries
             Metencephalon
     
     Thymus (Hematopoietic System)
             Thymus
     
     Gut Tube (Gastrointestinal Tract)
             Foregut
     
     Liver (Hepatobiliary System)
             Liver Lobule
    E2F4 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    E2F4 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.108371

    UniProtKB/Swiss-Prot: E2F4_HUMAN, Q16254
    Tissue specificity: Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle,
    kidney and pancreas

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for E2F4 gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia E2f41 , 5 E2F transcription factor 41, 5 88.1(n)1
    92.91(a)1
      8 (53.04 cM)5
    1043941  NM_148952.11  NP_683754.11 
     1052976635 
    chicken
    (Gallus gallus)
    Aves E2F41 E2F transcription factor 4, p107/p130-binding 73.23(n)
    77.66(a)
      769430  NM_001005835.1  NP_001005835.1 
    lizard
    (Anolis carolinensis)
    Reptilia E2F46
    E2F transcription factor 4, p107/p130-binding
    74(a)
    1 ↔ 1
    GL343295.1(415048-437626)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.247862 Xenopus laevis transcribed sequence with moderate similarity more 73.02(n)    BJ626522.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufb72f072 Danio rerio cDNA clone MGC63815 IMAGE3815782, complete more 75.1(n)    BC056832.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta E2f26
    E2F transcription factor 2
    26(a)
    1 → many
    2L(21154293-21156232)
    worm
    (Caenorhabditis elegans)
    Secernentea efl-13   -- 43(a)   V(16961540-16968261)   --


    ENSEMBL Gene Tree for E2F4 (if available)
    TreeFam Gene Tree for E2F4 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for E2F4 gene
    E2F12  E2F32  E2F22  E2F52  E2F62  
    3 SIMAP similar genes for E2F4 using alignment to 5 protein entries:     E2F4_HUMAN (see all proteins):
    E2F3    E2F6    E2F5

    E2F4 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for E2F4
    PGOHUM00000243616


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Polymorphic Variants from UniProtKB/Swiss-Prot
    E2F4_HUMAN, Q16254: The poly-Ser region of E2F4 is polymorphic and the number of Ser varies in the population (from 8 to
    17). The variation might be associated with tumorigenesis


    Selected SNPs for E2F4 (see all 148)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1431844931,2
    C--67226142(+) GAGGCC/TGGGCC 2 A syn10--------
    rs2001233451,2
    --67226171(+) GGGCAC/TTCCAA 2 T I mis10--------
    rs1811922941,2
    C--67226369(+) CGGCCG/TCCATC 1 -- int10--------
    rs37303931,2
    C,F,H--67226405(+) CGGGGG/AATGTT 1 -- int18Minor allele frequency- A:0.03NS NA 1172
    rs1412450851,2
    --67226426(+) GGCCGA/TGGCCA 1 -- int10--------
    rs2016935531,2
    --67226708(+) TACGAC/TATTAC 2 D syn10--------
    rs1123881451,2
    C--67226756(+) TCCAAA/C/GAACAG 3 K N syn1 mis11CSA 1
    rs3681015401,2
    C--67226791(+) AGTGGA/G/TAGGGT 1 -- int10--------
    rs1473836101,2
    C--67226803(+) GTAGAA/GTCTCC 1 -- int10--------
    rs2009205911,2
    --67226869(+) GACCCA/GGAGTT 1 -- int10--------

    HapMap Linkage Disequilibrium report for E2F4 (67226068 - 67232821 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for E2F4 (see all 13):    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv2887n71CNV Loss21882294
    dgv2888n71CNV Loss21882294
    dgv2889n71CNV Loss21882294
    nsv906794CNV Loss21882294
    nsv906803CNV Loss21882294
    dgv2886n71CNV Loss21882294
    nsv524492CNV Loss19592680
    nsv827707CNV Loss20364138
    nsv457513CNV Loss19166990
    dgv2885n71CNV Loss21882294

    Site Specific Mutation Identification with PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 600659    OMIM disorders: --

    Selected diseases for E2F4 (see all 31):    About MalaCards
    cervical intraepithelial neoplasia    retinoblastoma    labyrinthitis    glioblastoma multiforme
    burkitt's lymphoma    thyroid cancer    ovarian cancer    colorectal cancer
    endometrial carcinoma    astrocytoma    chronic lymphocytic leukemia    coronary heart disease
    hypoxia    pancreatic cancer    thyroiditis    cervicitis
    neuroblastoma    prostatitis    myeloma    breast cancer

    1 disease from the University of Copenhagen DISEASES database for E2F4:
    Retinoblastoma

    E2F4 for disorders           About GeneDecksing

    Selected Novoseek inferred disease relationships for E2F4 gene (see all 14)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    retinoblastoma 79.3 4 9139687 (1), 15548374 (1), 12674332 (1), 12754095 (1)
    microsatellite instability 57.7 1 10679953 (1)
    tumors 34.8 19 10797289 (3), 9824204 (2), 12373148 (2), 15867222 (2) (see all 12)
    cancer 24.5 8 10717241 (1), 9777938 (1), 9824204 (1), 15611646 (1) (see all 7)
    colon cancer 23.6 3 19562678 (2), 12373148 (1)
    colorectal cancer 18.7 9 12148576 (5), 19562678 (2), 10679953 (1), 11706064 (1)
    prostate carcinoma 18.4 2 15231644 (2)
    somatic mutations 10.8 2 9927063 (1), 10797289 (1)
    ovarian cancer 10.2 3 16638845 (2)
    breast carcinoma 5.32 2 11759817 (2)

    Genetic Association Database (GAD): E2F4
    Human Genome Epidemiology (HuGE) Navigator: E2F4 (3 documents)

    Export disorders for E2F4 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for E2F4 gene, integrated from 10 sources (see all 221):
    (articles sorted by number of sources associating them with E2F4)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle. (PubMed id 7892279)1, 2, 3, 9 Sardet C....Weinberg R.A. (Proc. Natl. Acad. Sci. U.S.A. 1995)
    2. E2F-4, a new member of the E2F transcription factor family, interacts with p107. (PubMed id 7958924)1, 2, 3, 9 Ginsberg D.... Livingston D.M. (Genes Dev. 1994)
    3. Genomic structure and mutation screening of the E2F4 gene in human tumors. (PubMed id 10797289)1, 2, 9 Schwemmle S. and Pfeifer G.P. (Int. J. Cancer 2000)
    4. Various AGC repeat numbers in the coding region of the human transcription factor gene E2F-4. (PubMed id 10679953)1, 2, 9 Zhong X.... Shimatake H. (Hum. Mutat. 2000)
    5. E2F transcriptional activation requires TRRAP and GCN5 cofactors. (PubMed id 11418595)1, 2, 9 Lang S.E.... Hearing P. (J. Biol. Chem. 2001)
    6. Genome-wide association study of coronary heart disease and its risk factors in 8,090 African Americans: the NHLBI CARe Project. (PubMed id 21347282)1, 4 Lettre G....Boerwinkle E. (PLoS Genet. 2011)
    7. Cell cycle genes and ovarian cancer susceptibility: a tagSNP analysis. (PubMed id 19738611)1, 4 Cunningham J.M....Goode E.L. (Br. J. Cancer 2009)
    8. Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations. (PubMed id 18660489)1, 4 Lu Y....Boer J.M. (J. Lipid Res. 2008)
    9. Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence. (PubMed id 17531812)1, 2 Litovchick L.... DeCaprio J.A. (Mol. Cell 2007)
    10. LINC, a human complex that is related to pRB-containing complexes in invertebrates regulates the expression of G2/M genes. (PubMed id 17671431)1, 2 Schmit F.... Gaubatz S. (Cell Cycle 2007)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 1874 HGNC: 3118 AceView: E2F4 Ensembl:ENSG00000205250 euGenes: HUgn1874
    ECgene: E2F4 Kegg: 1874 H-InvDB: E2F4

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for E2F4 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for E2F4 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/e2f4/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for E2F4 gene:
    Search GeneIP for patents involving E2F4

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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