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DUT Gene(Protein Coding)

Deoxyuridine Triphosphatase

GCID:
GC15P048331
GIFtS:
59
Genes Participants
UDN Logo

Aliases for DUT Gene

Aliases for DUT Gene

  • Deoxyuridine Triphosphatase 2 3 5
  • DUTP Pyrophosphatase 2 3 4
  • DUTP Diphosphatase 2 3
  • EC 3.6.1.23 4 61
  • DUTPase 3 4
  • Deoxyuridine 5-Triphosphate Nucleotidohydrolase, Mitochondrial 3
  • DUTP Nucleotidohydrolase 3

External Ids for DUT Gene

Previous GeneCards Identifiers for DUT Gene

  • GC15P044181
  • GC15P041669
  • GC15P046202
  • GC15P046339
  • GC15P046410
  • GC15P048623
  • GC15P025461

Summaries for DUT Gene

Entrez Gene Summary for DUT Gene

  • This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

GeneCards Summary for DUT Gene

DUT (Deoxyuridine Triphosphatase) is a Protein Coding gene. Diseases associated with DUT include Adamantinoma Of Long Bones. Among its related pathways are Purine metabolism (KEGG) and Pyrimidine metabolism. GO annotations related to this gene include poly(A) RNA binding and magnesium ion binding.

UniProtKB/Swiss-Prot for DUT Gene

  • This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Gene Wiki entry for DUT Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DUT Gene

Genomics for DUT Gene

Products:

  1. Regulatory Element

Regulatory Elements for DUT Gene

Enhancers for DUT Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH15G048682 1.8 FANTOM5 Ensembl ENCODE dbSUPER 14.4 +352.4 352397 1.9 ATF1 DMAP1 BRCA1 ZNF48 YY1 ZNF143 ZNF207 DEK REST ZNF592 DUT ENSG00000259488 FBN1 GC15M048674 LOC645405
GH15G048809 1.1 ENCODE 14.4 +479.9 479932 2.2 HDGF PKNOX1 CREB3L1 ARNT WRNIP1 SIN3A DMAP1 YY1 ZNF143 FOS DUT ENSG00000259488 CEP152 ENSG00000259700
GH15G049154 1.1 ENCODE 11.8 +824.7 824658 3.1 HDGF PKNOX1 MLX CREB3L1 ARNT WRNIP1 ARID4B SIN3A FEZF1 DMAP1 ENSG00000259488 DUT SECISBP2L FAM227B DTWD1 NDUFAF4P1 COPS2
GH15G048005 0.8 ENCODE 14.4 -324.9 -324934 1.0 GTF2F1 TBP PKNOX1 JUN MAX KLF17 BRCA1 ZIC2 RFX5 SMARCC2 DUT ENSG00000259488 GC15P048022 ENSG00000259730
GH15G048285 1 Ensembl ENCODE 11.2 -45.1 -45111 0.6 ELF3 CTCF BMI1 ZNF2 RAD21 ZBTB40 SCRT2 SMC3 FOS MAFK SLC12A1 ENSG00000202542 DUT PIR60543 GC15M048328
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around DUT on UCSC Golden Path with GeneCards custom track

Promoters for DUT Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000076158 889 3001 HDGF CREB3L1 ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF766 ZNF143

Transcription factor binding sites by QIAGEN in the DUT gene promoter:

Genomic Location for DUT Gene

Chromosome:
15
Start:
48,331,011 bp from pter
End:
48,343,373 bp from pter
Size:
12,363 bases
Orientation:
Plus strand

Genomic View for DUT Gene

Genes around DUT on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DUT Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DUT Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for DUT Gene

Proteins for DUT Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for DUT Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P33316-DUT_HUMAN
    Recommended name:
    Deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial
    Protein Accession:
    P33316
    Secondary Accessions:
    • A8K650
    • B4DPR5
    • O14785
    • Q16708
    • Q16860
    • Q6FHN1
    • Q6NSA3
    • Q96Q81

    Protein attributes for DUT Gene

    Size:
    252 amino acids
    Molecular mass:
    26563 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homotrimer.
    Miscellaneous:
    • Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each substrate molecule, residues from adjacent subunits contribute to the binding interactions.
    SequenceCaution:
    • Sequence=AAB71393.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305}; Sequence=AAB93866.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305}; Sequence=AAB94642.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for DUT Gene

    Alternative splice isoforms for DUT Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DUT Gene

Protein Expression for DUT Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Selected DME Specific Peptides for DUT Gene

P33316:
  • AAGYDLYSAYDYTIP
  • GGFGSTG
  • YGRVAPRSGLA
  • GVIDEDYRGN

Post-translational modifications for DUT Gene

  • Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
  • Ubiquitination at isoforms=363 and isoforms=391
  • Modification sites at PhosphoSitePlus

Other Protein References for DUT Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for DUT Gene

Protein Domains for DUT Gene

InterPro:
Blocks:
ProtoNet:

Suggested Antigen Peptide Sequences for DUT Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P33316

UniProtKB/Swiss-Prot:

DUT_HUMAN :
  • Belongs to the dUTPase family.
Family:
  • Belongs to the dUTPase family.
genes like me logo Genes that share domains with DUT: view

No data available for Gene Families for DUT Gene

Function for DUT Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line
  7. Flow Cytometry

Molecular function for DUT Gene

GENATLAS Biochemistry:
dUTP pyrophosphatase,involved in DNA replication
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=2.5 uM for dUTP {ECO:0000269 PubMed:8631816}; Note=for both isoform 2 and isoform 3.;
UniProtKB/Swiss-Prot CatalyticActivity:
dUTP + H(2)O = dUMP + diphosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Phosphorylation is necessary for activity.
UniProtKB/Swiss-Prot Function:
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Enzyme Numbers (IUBMB) for DUT Gene

Gene Ontology (GO) - Molecular Function for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding IDA 22658674
GO:0004170 dUTP diphosphatase activity IEA,EXP 8631816
GO:0005515 protein binding IPI 16189514
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with DUT: view
genes like me logo Genes that share phenotypes with DUT: view

Animal Model Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for DUT Gene

Localization for DUT Gene

Subcellular locations from UniProtKB/Swiss-Prot for DUT Gene

Isoform 2: Nucleus.
Isoform 3: Mitochondrion.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DUT gene
Compartment Confidence
extracellular 5
mitochondrion 5
nucleus 5
peroxisome 2
cytosol 1

Gene Ontology (GO) - Cellular Components for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 8631816
GO:0005654 nucleoplasm TAS,IDA --
GO:0005739 mitochondrion TAS,IEA --
GO:0070062 extracellular exosome IDA 20458337
genes like me logo Genes that share ontologies with DUT: view

Pathways & Interactions for DUT Gene

genes like me logo Genes that share pathways with DUT: view

UniProtKB/Swiss-Prot P33316-DUT_HUMAN

  • Pathway: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.

Gene Ontology (GO) - Biological Process for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006139 nucleobase-containing compound metabolic process TAS 1325640
GO:0006226 dUMP biosynthetic process IEA --
GO:0006260 DNA replication TAS 1325640
GO:0009117 nucleotide metabolic process IEA --
GO:0015949 nucleobase-containing small molecule interconversion TAS --
genes like me logo Genes that share ontologies with DUT: view

No data available for SIGNOR curated interactions for DUT Gene

Drugs & Compounds for DUT Gene

Products:

  1. Drug

(8) Drugs for DUT Gene - From: DrugBank, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
1-{(2S,5S)-4-FLUORO-5-[(TRITYLOXY)METHYL]TETRAHYDROFURAN-2-YL}PYRIMIDINE-2,4(1H,3H)-DIONE Experimental Pharma Target 0
Deoxyuridine-5'-Diphosphate Experimental Pharma Target 0
Magnesium Nutra 0

(16) Additional Compounds for DUT Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • (4-)Diphosphoric acid ion
  • (P2O74-)Diphosphate
  • Diphosphate
  • Diphosphoric acid
  • PPi
 14000-31-8
Deoxyuridine triphosphate
  • 2'-Deoxyuridine 5'-triphosphate
  • 2'-Deoxyuridine-5'-triphosphorate
  • 2'-Deoxyuridine-5'-triphosphoric acid
  • 2'-Deoxyuridine-5'-triphosphoric acid = dUTP
  • Deoxy-UTP
 1173-82-6
dUMP
  • 2'-Deoxy-5'-uridylate
  • 2'-Deoxy-5'-uridylic acid
  • 2'-Deoxyuridine 5'-monophosphate
  • 2'-Deoxyuridine 5'-phosphate
  • 2'-Deoxyuridylate
 964-26-1
genes like me logo Genes that share compounds with DUT: view

Transcripts for DUT Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone
  5. Flow Cytometry

mRNA/cDNA for DUT Gene

(6) REFSEQ mRNAs :
(15) Additional mRNA sequences :
(417) Selected AceView cDNA sequences:
(11) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for DUT Gene

Deoxyuridine triphosphatase:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for DUT Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c · 2d · 2e · 2f · 2g ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b
SP1: - - - - - - - - - -
SP2: - - - - -
SP3: - - -
SP4: - -
SP5: - - - - - - - - -
SP6: - -
SP7: - - -
SP8: -
SP9: - -

Relevant External Links for DUT Gene

GeneLoc Exon Structure for
DUT
ECgene alternative splicing isoforms for
DUT

Expression for DUT Gene

Products:

  1. Primer

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for DUT Gene

mRNA expression in normal human tissues for DUT Gene
mRNA expression by BioGPS for DUT GenemRNA expression by GTEx for DUT Gene

Protein differential expression in normal tissues from HIPED for DUT Gene

This gene is overexpressed in Peripheral blood mononuclear cells (10.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for DUT Gene



Protein tissue co-expression partners for DUT Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of DUT Gene:

DUT

SOURCE GeneReport for Unigene cluster for DUT Gene:

Hs.527980

mRNA Expression by UniProt/SwissProt for DUT Gene:

P33316-DUT_HUMAN
Tissue specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).

Evidence on tissue expression from TISSUES for DUT Gene

  • Nervous system(4.8)
  • Lung(4.6)
  • Liver(4.4)
  • Blood(4.3)
  • Eye(4.2)
  • Intestine(2.1)
genes like me logo Genes that share expression patterns with DUT: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for DUT Gene

Orthologs for DUT Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for DUT Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
 Mammalia DUT 34 35
  • 98.94 (n)
cow
(Bos Taurus)
 Mammalia DUT 34 35
  • 89.41 (n)
-- 35
  • 82 (a)
 OneToMany
dog
(Canis familiaris)
 Mammalia DUT 34 35
  • 84.66 (n)
oppossum
(Monodelphis domestica)
 Mammalia DUT 35
  • 83 (a)
 OneToOne
mouse
(Mus musculus)
 Mammalia Dut 34 16 35
  • 81.53 (n)
platypus
(Ornithorhynchus anatinus)
 Mammalia -- 35
  • 81 (a)
 OneToMany
-- 35
  • 81 (a)
 OneToMany
rat
(Rattus norvegicus)
 Mammalia Dut 34
  • 80.86 (n)
chicken
(Gallus gallus)
 Aves DUT 34 35
  • 75.97 (n)
lizard
(Anolis carolinensis)
 Reptilia DUT 35
  • 65 (a)
 OneToOne
tropical clawed frog
(Silurana tropicalis)
 Amphibia LOC101733437 34
  • 66.28 (n)
Str.1294 34
African clawed frog
(Xenopus laevis)
 Amphibia Xl.7472 34
zebrafish
(Danio rerio)
 Actinopterygii dut 34 35
  • 65.4 (n)
wufa28d11 34
rainbow trout
(Oncorhynchus mykiss)
 Actinopterygii Omy.2007 34
African malaria mosquito
(Anopheles gambiae)
 Insecta AgaP_AGAP010159 34
  • 65.95 (n)
fruit fly
(Drosophila melanogaster)
 Insecta dUTPase 34 35
  • 58.96 (n)
worm
(Caenorhabditis elegans)
 Secernentea K07A1.2 36
  • 58 (a)
dut-1 34 35
  • 57.17 (n)
A. gosspyii yeast
(Ashbya gossypii)
 Saccharomycetes AGOS_AGR249C 34
  • 54.5 (n)
baker's yeast
(Saccharomyces cerevisiae)
 Saccharomycetes DUT1 35
  • 52 (a)
 OneToOne
sea squirt
(Ciona savignyi)
 Ascidiacea -- 35
  • 67 (a)
 OneToMany
CSA.6436 35
  • 62 (a)
 OneToMany
sea squirt
(Ciona intestinalis)
 Ascidiacea Cin.2142 34
Species where no ortholog for DUT was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DUT Gene

ENSEMBL:
Gene Tree for DUT (if available)
TreeFam:
Gene Tree for DUT (if available)

Paralogs for DUT Gene

No data available for Paralogs for DUT Gene

Variants for DUT Gene

Sequence variations from dbSNP and Humsavar for DUT Gene

SNP ID Clin Chr 15 pos Sequence Context AA Info Type
rs1000573130 -- 48,340,162(+) GGGTA(C/G)AGTAT intron-variant, utr-variant-3-prime
rs1000625389 -- 48,340,411(+) TGTAA(C/T)ACTTC intron-variant, utr-variant-3-prime
rs1000785048 -- 48,333,216(+) AACAT(G/T)AGAAC intron-variant
rs1001102963 -- 48,340,836(+) GTCAG(C/T)GTTGA intron-variant, downstream-variant-500B
rs1001133842 -- 48,332,947(+) GTTGT(A/T)TTTAG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DUT Gene

Variant ID Type Subtype PubMed ID
esv2760030 CNV loss 17122850
esv3569565 CNV gain 25503493
esv3636412 CNV gain 21293372
esv3636413 CNV loss 21293372
nsv1053885 CNV gain 25217958

Variation tolerance for DUT Gene

Residual Variation Intolerance Score: 42.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.69; 14.60% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for DUT Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
DUT

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DUT Gene

Disorders for DUT Gene

MalaCards: The human disease database

(1) MalaCards diseases for DUT Gene - From: Novoseek and GeneCards

Disorder Aliases PubMed IDs
adamantinoma of long bones
  • long bone adamantinoma
- elite association - COSMIC cancer census association via MalaCards
Search DUT in MalaCards View complete list of genes associated with diseases

Relevant External Links for DUT

Genetic Association Database (GAD)
DUT
Human Genome Epidemiology (HuGE) Navigator
DUT
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
DUT
genes like me logo Genes that share disorders with DUT: view

No data available for UniProtKB/Swiss-Prot and Genatlas for DUT Gene

Publications for DUT Gene

  1. Assignment of the human dUTPase gene (DUT) to chromosome 15q15-q21. 1 by fluorescence in situ hybridization. (PMID: 9070952) Cohen D. … Pearlman R.E. (Genomics 1997) 2 3 4 22 64
  2. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. (PMID: 1325640) McIntosh E.M. … Haynes R.H. (Proc. Natl. Acad. Sci. U.S.A. 1992) 2 3 4 22 64
  3. Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase. (PMID: 17880943) Varga B. … Vertessy B.G. (FEBS Lett. 2007) 3 4 22 64
  4. The human dUTPase gene encodes both nuclear and mitochondrial isoforms. Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. (PMID: 9228092) Ladner R.D. … Caradonna S.J. (J. Biol. Chem. 1997) 3 4 22 64
  5. Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase. (PMID: 8631816) Ladner R.D. … Caradonna S.J. (J. Biol. Chem. 1996) 3 4 22 64

Products for DUT Gene

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