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DUT Gene

protein-coding GIFtS: 67
GCID: GC15P048623

Deoxyuridine Triphosphatase

(Previous name: dUTP pyrophosphatase)

See DUT-related disease
at

MalaCards

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
About This Section

Aliases
Deoxyuridine Triphosphatase¹ ²
DUTP Pyrophosphatase¹ ² ³
dUTPase² ³
EC 3.6.1.23³ ⁸
Deoxyuridine 5'-Triphosphate Nucleotidohydrolase, Mitochondrial²
DUTP Nucleotidohydrolase²

External Ids:	HGNC: 3078¹	Entrez Gene: 1854²	Ensembl: ENSG00000128951⁷	OMIM: 601266⁵	UniProtKB: P33316³

Export aliases for DUT gene to outside databases

Previous GC identifers: GC15P044181 GC15P041669 GC15P046202 GC15P046339 GC15P046410 GC15P025461

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for DUT Gene:

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous,

homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes:

providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting

intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which

induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal

and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative

splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related

pseudogene is located on chromosome 19. (provided by RefSeq, Jul 2008)

GeneCards Summary for DUT Gene:

DUT (deoxyuridine triphosphatase) is a protein-coding gene. Diseases associated with DUT include cysticercosis.

UniProtKB/Swiss-Prot: DUT_HUMAN, P33316

Function: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine

nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into

DNA

Gene Wiki entry for DUT Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence at NCBI GenBank:

NC_000015.9 NT_010194.18 NC_018926.2

Regulatory elements:

Regulatory transcription factor binding sites in the DUT gene promoter:
AML1a p53 POU2F1a
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): DUT promoter sequence

Search Chromatin IP Primers for DUT

Epigenetics:

DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat DUT

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q21.1 Ensembl cytogenetic band: 15q21.1 HGNC cytogenetic band: 15q21.1

DUT Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DUT gene location

GeneLoc information about chromosome 15 GeneLoc Exon Structure

GeneLoc location for GC15P048623: view genomic region (about GC identifiers)

Start:	48,623,208 bp from pter	End:	48,635,570 bp from pter
Size:	12,363 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, ²neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, antibodies-online, and/or GeneTex,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
About This Section

UniProtKB/Swiss-Prot: DUT_HUMAN, P33316 (See protein sequence)

Recommended Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial precursor

Size: 252 amino acids; 26563 Da

Cofactor: Magnesium

Subunit: Homotrimer

Miscellaneous: Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each

substrate molecule, residues from adjacent subunits contribute to the binding interactions

Sequence caution: Sequence=AAB71393.1; Type=Frameshift; Positions=29, 47; Sequence=AAB93866.1; Type=Frameshift;

Positions=29, 47; Sequence=AAB94642.1; Type=Frameshift; Positions=29, 47;

Selected PDB 3D structures from and Proteopedia for DUT (see all 8):

1Q5H (3D)

1Q5U (3D)

2HQU (3D)

3ARA (3D)

3ARN (3D)

3EHW (3D)

Secondary accessions: A8K650 B4DPR5 O14785 Q16708 Q16860 Q6FHN1 Q6NSA3 Q96Q81

Alternative splicing: 2 isoforms: P33316-3 P33316-2 (Major isoform. Phosphorylated in Ser-11. Contains a phosphoserine at position 11)

Explore the universe of human proteins at neXtProt for DUT: NX_P33316

Explore proteomics data for DUT at MOPED

Post-translational modifications:

Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2.

Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated¹

The initiator methionine is cleaved in isoform 2¹

Ubiquitination² at Lys14, Lys63, Lys91, Lys163

Modification sites at PhosphoSitePlus

4 DME Specific Peptides for DUT (P33316)

GGFGSTG

GVIDEDYRGN

YGRVAPRSGLA

AAGYDLYSAYDYTIP

See DUT Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins (3 alternative transcripts):

NP_001020419.1 NP_001020420.1 NP_001939.1

ENSEMBL proteins:

ENSP00000453667 ENSP00000454183 ENSP00000453717 ENSP00000370376 ENSP00000452749

ENSP00000452814 ENSP00000405160 ENSP00000454041 ENSP00000453683

Reactome Protein details: P33316

DUT Human Recombinant Protein Products:

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	antibodies-online proteins for DUT (87 products)
	antibodies-online peptides for DUT

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DUT Assay Products:

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	antibodies-online kits for DUT (2 products)

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GenesLikeMe)
About This Section

2 InterPro protein domains:

IPR008180 dUTP_pyroPase

IPR008181 dUTP_pyroPase_sf

Graphical View of Domain Structure for InterPro Entry P33316

ProtoNet protein and cluster: P33316

2 Blocks protein domains:

IPB003232 dCTP Deaminase

IPB008180 DeoxyUTP pyrophosphatase

UniProtKB/Swiss-Prot: DUT_HUMAN, P33316

Similarity: Belongs to the dUTPase family

Find genes that share domains with DUT About GenesLikeMe

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: DUT_HUMAN, P33316

Function: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine

nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into

DNA

Catalytic activity: dUTP + H(2)O = dUMP + diphosphate

Enzyme regulation: Phosphorylation is necessary for activity

Biophysicochemical properties: Kinetic parameters: KM=2.5 uM for dUTP; Note=for both isoform 2 and isoform 3;

Genatlas biochemistry entry for DUT:

dUTP pyrophosphatase,involved in DNA replication

Enzyme Number (IUBMB): EC 3.6.1.23¹ ²

Gene Ontology (GO): 4 molecular function terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004170	dUTP diphosphatase activity	EXP	--
GO:0005515	protein binding	IPI	16189514
GO:0016787	hydrolase activity	--	--
GO:0044822	poly(A) RNA binding	IDA	--

Find genes that share ontologies with DUT About GenesLikeMe

Phenotypes:
2 GenomeRNAi human phenotypes for DUT:

Decreased viability

Increased cell transformation

Animal Models:

genOway: Develop your customized and physiologically relevant rodent model for DUT

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In Situ Assay
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Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DUT

(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
About This Section

Subcellular locations from UniProtKB/Swiss-Prot

DUT_HUMAN, P33316: Isoform 2: Nucleus

DUT_HUMAN, P33316: Isoform 3: Mitochondrion

Subcellular locations from COMPARTMENTS:

Compartment	Confidence
mitochondrion	5
nucleus	5
peroxisome	2
cytosol	1

About this image

Gene Ontology (GO): 5 cellular component terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005654	nucleoplasm	TAS	--
GO:0005730	NOT nucleolus	IDA	--
GO:0005739	mitochondrion	IEA	--
GO:0070062	extracellular vesicular exosome	IDA	--

Find genes that share ontologies with DUT About GenesLikeMe

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GenesLikeMe, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene, Sets of similar genes according to GenesLikeMe)
About This Section

SuperPaths for DUT About

See pathways by source

Powered
by

SuperPath

Contained pathways About

Metabolism

Metabolism

0.38

Metabolic pathways

0.38

superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis

superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis

0.52

pyrimidine deoxyribonucleotides de novo biosynthesis

0.00

Pyrimidine metabolism

Pyrimidine metabolism

0.33

Pyrimidine biosynthesis

0.00

Purine metabolism (REACTOME)

Metabolism of nucleotides

0.42

Purine metabolism (KEGG)

Pyrimidine metabolism

0.38

Find genes that share SuperPaths with DUT About GenesLikeMe

Pathways by source See SuperPaths
Show all pathways

2 BioSystems Pathways for DUT

	superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
	pyrimidine deoxyribonucleotides de novo biosynthesis

1 Reactome Pathway for DUT

Pyrimidine biosynthesis

2 Kegg Pathways (Kegg details for DUT):

	Pyrimidine metabolism
	Metabolic pathways

UniProtKB/Swiss-Prot: DUT_HUMAN, P33316

Pathway: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2

Custom Pathway & Disease-focused RT² Profiler PCR Arrays for DUT
Interactions:

GeneGlobe Interaction Network for DUT

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

Selected Interacting proteins for DUT (P33316^{1, 2, 3} ENSP00000370376⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 78)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
NUDT18	Q6ZVK8¹^,²^,³, ENSP00000307852⁴	EBI-353224,EBI-740486 MINT-68046 I2D: score=4 STRING: ENSP00000307852
SPATA2	Q9UM82²^,³, ENSP00000289431⁴	MINT-66521 I2D: score=4 STRING: ENSP00000289431
RHOB	P62745³, ENSP00000272233⁴	I2D: score=2 STRING: ENSP00000272233
GH1	P01241³, ENSP00000312673⁴	I2D: score=1 STRING: ENSP00000312673
PPARA	Q07869³, ENSP00000262735⁴	I2D: score=1 STRING: ENSP00000262735

About this table

Gene Ontology (GO): Selected biological process terms (see all 8): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006139	nucleobase-containing compound metabolic process	TAS	1325640
GO:0006206	pyrimidine nucleobase metabolic process	TAS	--
GO:0006226	dUMP biosynthetic process	IEA	--
GO:0006260	DNA replication	TAS	1325640
GO:0044281	small molecule metabolic process	TAS	--

Find genes that share ontologies with DUT About GenesLikeMe

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, Sets of similar genes according to GenesLikeMe)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse compounds at ApexBio

Browse Tocris compounds for DUT

6 HMDB Compounds for DUT About this table

Compound	Synonyms	CAS #	PubMed Ids
Deoxyuridine triphosphate	2'-Deoxyuridine-5'-triphosphorate (see all 9)	1173-82-6	--
Magnesium	Magnesium (see all 2)	7439-95-4	--
Phosphoric acid	acide phosphorique (FRENCH) (see all 20)	7664-38-2	--
Pyrophosphate	(4-)Diphosphoric acid ion (see all 10)	14000-31-8	--
Water	Dihydrogen oxide (see all 2)	7732-18-5	--
dUMP	2'-Deoxy-5'-uridylate (see all 12)	964-26-1	--

2 DrugBank Compounds for DUT About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
Deoxyuridine-5'-Diphosphate	--	--	target	--	17139284 17016423
1-{(2S,5S)-4-FLUORO-5-[(TRITYLOXY)METHYL]TETRAHYDROFURAN-2-YL}PYRIMIDINE-2,4(1H,3H)-DIONE	--	--	target	--	10592235

Selected Novoseek inferred chemical compound relationships for DUT gene (see all 18) About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
dutp	91.6	149	8923782 (3), 15322254 (3), 8805593 (3), 10329142 (3) (see all 45)
deoxyuridine	85.7	30	19052026 (2), 8595399 (2), 8013732 (2), 8221659 (2) (see all 15)
dudp	83.8	7	17932923 (3), 11420444 (1), 8798636 (1)
dttp	80.7	3	12369927 (1), 10329142 (1)
2'-deoxyuridine-5-monophosphate	78.9	3	2153555 (1), 10329142 (1)
zd9331	78	6	10952785 (3), 11487279 (1)
thymidylate	75.1	22	15322254 (3), 10952785 (2), 10698291 (2), 18519027 (2) (see all 9)
pyrophosphate	64.5	14	12369927 (1), 17452782 (1), 8805593 (1), 2153555 (1) (see all 9)
fudr	61.8	14	7652165 (2), 8162567 (2), 15322254 (1), 8221659 (1) (see all 5)
cytosine	48.7	1	1694967 (1)

Find genes that share compounds with DUT About GenesLikeMe

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
About This Section

REFSEQ mRNAs for DUT gene (4 alternative transcripts):

NM_001025248.1 NM_001025249.1 NM_001948.3 XM_005254211.1

Unigene Cluster for DUT:

Deoxyuridine triphosphatase

Hs.527980 [show with all ESTs]

Unigene Representative Sequence: NM_001025248

11 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000559935 ENST00000559416 ENST00000558813 ENST00000331200(uc001zws.3 uc001zwt.3)

ENST00000558472 ENST00000558978 ENST00000455976(uc001zww.3) ENST00000559540

ENST00000558367 ENST00000561350 ENST00000559852

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		SwitchGear 3'UTR luciferase reporter plasmid: DUT 3' UTR sequence

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		QuantiFast Probe-based Assays in human, mouse, rat DUT

Additional mRNA sequence:

AB049113.2 AK000629.1 AK291515.1 AK298464.1 AK312122.1 BC033645.1 BC070339.1 BC110377.1

CR541720.1 CR541781.1 L11877.1 M89913.1 U31930.1 U62891.1 U90223.1

15 DOTS entries:

DT.452923 DT.95377446 DT.100823718 DT.100823711 DT.91774168 DT.91774170 DT.100823709 DT.100823721

DT.218148 DT.91774169 DT.97764571 DT.95377854 DT.100035287 DT.100823717 DT.121072702

Selected AceView cDNA sequences (see all 417):

BU677665 AI570675 BQ777742 CB529208 AA262687 AW160384 BI438412 AA284034

BG654389 CR607847 CR541720 AA830251 AA720503 CD677395 BE551158 CB133275

AA027178 AW162006 BM757904 BU729937 NM_001948 BI333317 CA866197 BM840182

GeneLoc Exon Structure

Selected Alternative Splicing Database (ASD) splice patterns (SP) for DUT (see all 9) About this scheme

ExUns:	1a	·	1b	^	2a	·	2b	·	2c	·	2d	·	2e	·	2f	·	2g	^	3a	·	3b	^	4	^	5a	·	5b	^	6	^	7	^	8	^	9	^	10a	·	10b
SP1:					-		-		-		-		-		-		-		-				-										-
SP2:															-		-		-				-										-
SP3:																			-				-										-
SP4:																			-				-
SP5:					-		-		-		-		-		-		-		-				-

ECgene alternative splicing isoforms for DUT

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GenesLikeMe, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

DUT expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>^2/3
CGAP TAG: CAGCTCCGCT

About this image

DUT expression in embryonic tissues and stem cells About this table

Data from LifeMap, the Embryonic Development and Stem Cells Database
selected tissues (see all 15) fully expand


Inner Cell Mass (Early Embryonic Tissues) fully expand to see all 2 entries
Human embryonic stem cells (family)

Brain (Nervous System) fully expand to see all 2 entries
Cerebral Cortex

Ovary (Reproductive System)
Oviduct

Bone (Muscoskeletal System)
Bone Marrow

Trophoblast (Extraembryonic Tissues)
Trophoblast Cells Trophoblast

DUT Protein expression data from MOPED¹, PaxDb² and MaxQB³:

About this image

DUT Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.527980

UniProtKB/Swiss-Prot: DUT_HUMAN, P33316

Tissue specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2

expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with

the cessation of nuclear DNA replication (at protein level)

Custom PCR Arrays for DUT

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In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DUT

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals and fungi.

Orthologs for DUT gene from Selected species (see all 20) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Dut^{1 , 5}	deoxyuridine triphosphatase^{1, 5}	81.53(n)¹ 87.26(a)¹		2 (61.33 cM)⁵ 110074¹ NM_001159646.1¹ NP_001153118.1¹ 125247190⁵
chicken (Gallus gallus)	Aves	DUT¹	deoxyuridine triphosphatase	75.97(n) 87.01(a)		769338 NM_001079488.1 NP_001072956.1
lizard (Anolis carolinensis)	Reptilia	DUT⁶	deoxyuridine triphosphatase	65(a)	1 ↔ 1	GL343806.1(24610-36072)
African clawed frog (Xenopus laevis)	Amphibia	Xl.7472²	Xenopus laevis transcribed sequence with moderate similarity more	74.45(n)		48047642
zebrafish (Danio rerio)	Actinopterygii	wufa28d11²	Transcribed sequence with moderate similarity to protein more	73.7(n)		CK354832.1
fruit fly (Drosophila melanogaster)	Insecta	dUTPase¹	Deoxyuridine triphosphatase	58.96(n) 65.31(a)		34529 NM_135635.3 NP_609479.1
worm (Caenorhabditis elegans)	Secernentea	K07A1.2³ dut-1¹	deoxyuridine 5'-triphosphate nucleotidohydrolase³ dut-1¹	58(a)³ 57.17(n)¹ 60.61(a)¹		I(9593478-9595191)³ 172794¹ NM_001026382.4¹ NP_001021553.1¹
baker's yeast (Saccharomyces cerevisiae)	Saccharomycetes	DUT1⁶	deoxyuridine triphosphate diphosphatase (dUTPase);...	52(a)	1 ↔ 1	II(722611-723054) YBR252W

ENSEMBL Gene Tree for DUT (if available)
TreeFam Gene Tree for DUT (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68,Sets of similar genes according to GenesLikeMe)
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Paralogs for DUT gene

Find genes that share paralogs with DUT About GenesLikeMe

Selected Pseudogenes.org Pseudogenes for DUT (see all 9)
PGOHUM00000262119 PGOHUM00000245069 PGOHUM00000238159 PGOHUM00000262001 PGOHUM00000246007

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, ⁴UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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SNP ID	Valid	Clinical significance	Chr 15 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs181282904^1,2		--	25454653(+)	`TAAAAC/TGAGAG`	2	--	us2k¹		0	--	--	--	--
rs200629984^1,2		--	25455646(+)	`ATCTT-/TAAGTAC`	3	--	us2k¹		0	--	--	--	--
rs201166131^1,2		--	25455648(+)	`CTTAG-/TACTACAA`	3	--	cds¹		0	--	--	--	--
rs28381089^1,2	F	--	25455652(+)	`AGTAC-/TACAAGCA`	3	--	cds¹		5		NS	188
rs28381102^1,2	F	--	25457103(+)	`GGGGGG/CGTGGG`	3	--	int¹ us2k¹		5		NS	186
rs184273452^1,2		--	28687828(+)	`AGAAGA/GTTCAA`	2	--	us2k¹		0	--	--	--	--
rs3759912^1,2	C	--	28687972(+)	`tgggcA/Ggatca`	2	--	us2k¹		4		NA WA	8
rs190290692^1,2	C	--	28688111(+)	`AATTGC/TTTGAA`	2	--	us2k¹		0	--	--	--	--
rs8039967^1,2	A	--	28688189(+)	`aaaaaA/Caaaaa`	2	--	us2k¹		0	--	--	--	--
rs8039971^1,2	A	--	28688195(+)	`aaaaaA/CTATGA`	2	--	us2k¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for DUT (48623208 - 48635570 bp)

Structural Variations
Database of Genomic Variants (DGV) 1 variation for DUT: About this table

Variant ID	Type	Subtype	PubMed ID
dgv746e1	CNV	Complex	17122850

Site Specific Mutation Identification with PCR Assays

SeqTarget long-range PCR primers for resequencing DUT

DNA2.0 Custom Variant and Variant Library Synthesis for DUT

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB, Sets of similar genes according to GenesLikeMe)
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OMIM gene information: 601266 OMIM disorders: --

1 disease for DUT:
About MalaCards

cysticercosis

Find genes that share disorders with DUT About GenesLikeMe

Selected Novoseek inferred disease relationships for DUT gene (see all 11) About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
visna	56.2	1	9847382 (1)
ebv infections	38.4	4	19124728 (1)
herpes simplex	35.2	6	2168898 (1), 8391186 (1), 9400986 (1), 10774198 (1)
colorectal tumors	24.7	1	8162567 (1)
neoplastic disease	18.6	1	12376965 (1)
colon cancer	2.21	4	10910061 (2), 12374095 (1)
breast cancer	0	1	18790783 (1)
hiv infections	0	2	10329142 (1), 10752557 (1)
tumors	0	9	10910061 (2), 17955371 (1), 18837522 (1), 16325515 (1) (see all 5)
cancer	0	12	15322254 (2), 17880943 (1), 20084279 (1), 9360704 (1) (see all 10)

Genetic Association Database (GAD): DUT
Human Genome Epidemiology (HuGE) Navigator: DUT (3 documents)

Export disorders for DUT gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for DUT gene, integrated from 10 sources (see all 149):
(articles sorted by number of sources associating them with DUT)

Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. (PubMed id 1325640)^{1, 2, 3, 9} McIntosh E.M.... Haynes R.H. (Proc. Natl. Acad. Sci. U.S.A. 1992)
Assignment of the human dUTPase gene (DUT) to chromosome 15q15-q21. 1 by fluorescence in situ hybridization. (PubMed id 9070952)^{1, 2, 3, 9} Cohen D....Pearlman R.E. (Genomics 1997)
Identification of a consensus cyclin-dependent kinase phosphorylation site unique to the nuclear form of human deoxyuridine triphosphate nucleotidohydrolase. (PubMed id 8631817)^{1, 2, 9} Ladner R.D.... Caradonna S.J. (J. Biol. Chem. 1996)
Human dUTP pyrophosphatase: uracil recognition by a beta hairpin and active sites formed by three separate subunits. (PubMed id 8805593)^{1, 2, 9} Mol C.D.... Tainer J.A. (Structure 1996)
Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase. (PubMed id 8631816)^{1, 2, 9} Ladner R.D.... Caradonna S.J. (J. Biol. Chem. 1996)
Maturation stage and proliferation-dependent expression of dUTPase in human T cells. (PubMed id 8389461)^{1, 2, 9} Strahler J.R.... Hanash S.M. (Proc. Natl. Acad. Sci. U.S.A. 1993)
The human dUTPase gene encodes both nuclear and mitochondrial isoforms. Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. (PubMed id 9228092)^{1, 2, 9} Ladner R.D. and Caradonna S.J. (J. Biol. Chem. 1997)
Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase. (PubMed id 17880943)^{1, 2, 9} Varga B.... Vertessy B.G. (FEBS Lett. 2007)
Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PubMed id 20877624)^{1, 4} Hendrickson S.L....O'Brien S.J. (PLoS ONE 2010)
Polymorphisms in uracil-processing genes, but not one-carbon nutrients, are associated with altered DNA uracil concentrations in an urban Puerto Rican population. (PubMed id 19403629)^{1, 4} Chanson A....Mason J.B. (Am. J. Clin. Nutr. 2009)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
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Free Text

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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 1854	HGNC: 3078	AceView: DUT	Ensembl:ENSG00000128951	euGenes: HUgn1854
ECgene: DUT	Kegg: 1854	H-InvDB: DUT

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for DUT	Pharmacogenomics, SNPs, Pathways
NIEHS-SNPs	http://egp.gs.washington.edu/data/dut/

Intellectual Property for DUT gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for DUT gene:
Search GeneIP for patents involving DUT

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

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Deoxyuridine Triphosphatase

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