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Aliases for DUT Gene

Aliases for DUT Gene

  • Deoxyuridine Triphosphatase 2 3 5
  • DUTP Pyrophosphatase 2 3 4
  • DUTP Diphosphatase 2 3
  • EC 3.6.1.23 4 61
  • DUTPase 3 4
  • Deoxyuridine 5-Triphosphate Nucleotidohydrolase, Mitochondrial 3
  • DUTP Nucleotidohydrolase 3

External Ids for DUT Gene

Previous GeneCards Identifiers for DUT Gene

  • GC15P044181
  • GC15P041669
  • GC15P046202
  • GC15P046339
  • GC15P046410
  • GC15P048623
  • GC15P025461

Summaries for DUT Gene

Entrez Gene Summary for DUT Gene

  • This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

GeneCards Summary for DUT Gene

DUT (Deoxyuridine Triphosphatase) is a Protein Coding gene. Among its related pathways are superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis and Purine metabolism. GO annotations related to this gene include poly(A) RNA binding and magnesium ion binding.

UniProtKB/Swiss-Prot for DUT Gene

  • This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Gene Wiki entry for DUT Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DUT Gene

Genomics for DUT Gene

Regulatory Elements for DUT Gene

Enhancers for DUT Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
- Elite enhancer/Elite enhancer-gene association

Enhancers around DUT on UCSC Golden Path with GeneCards custom track

Promoters for DUT Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around DUT on UCSC Golden Path with GeneCards custom track

Genomic Location for DUT Gene

Chromosome:
15
Start:
48,331,011 bp from pter
End:
48,343,373 bp from pter
Size:
12,363 bases
Orientation:
Plus strand

Genomic View for DUT Gene

Genes around DUT on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DUT Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DUT Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DUT Gene

Proteins for DUT Gene

  • Protein details for DUT Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P33316-DUT_HUMAN
    Recommended name:
    Deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial
    Protein Accession:
    P33316
    Secondary Accessions:
    • A8K650
    • B4DPR5
    • O14785
    • Q16708
    • Q16860
    • Q6FHN1
    • Q6NSA3
    • Q96Q81

    Protein attributes for DUT Gene

    Size:
    252 amino acids
    Molecular mass:
    26563 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homotrimer.
    Miscellaneous:
    • Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each substrate molecule, residues from adjacent subunits contribute to the binding interactions.
    SequenceCaution:
    • Sequence=AAB71393.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305}; Sequence=AAB93866.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305}; Sequence=AAB94642.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for DUT Gene

    Alternative splice isoforms for DUT Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DUT Gene

Selected DME Specific Peptides for DUT Gene

Post-translational modifications for DUT Gene

  • Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
  • Ubiquitination at Lys 63 and Lys 91
  • Modification sites at PhosphoSitePlus

Domains & Families for DUT Gene

Suggested Antigen Peptide Sequences for DUT Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P33316

UniProtKB/Swiss-Prot:

DUT_HUMAN :
  • Belongs to the dUTPase family.
Family:
  • Belongs to the dUTPase family.
genes like me logo Genes that share domains with DUT: view

No data available for Gene Families for DUT Gene

Function for DUT Gene

Molecular function for DUT Gene

GENATLAS Biochemistry:
dUTP pyrophosphatase,involved in DNA replication
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=2.5 uM for dUTP {ECO:0000269 PubMed:8631816}; Note=for both isoform 2 and isoform 3.;
UniProtKB/Swiss-Prot CatalyticActivity:
dUTP + H(2)O = dUMP + diphosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Phosphorylation is necessary for activity.
UniProtKB/Swiss-Prot Function:
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Enzyme Numbers (IUBMB) for DUT Gene

Gene Ontology (GO) - Molecular Function for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IBA --
GO:0004170 dUTP diphosphatase activity EXP 8631816
GO:0005515 protein binding IPI 16189514
GO:0044822 poly(A) RNA binding IDA 22658674
genes like me logo Genes that share ontologies with DUT: view
genes like me logo Genes that share phenotypes with DUT: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for DUT Gene

Localization for DUT Gene

Subcellular locations from UniProtKB/Swiss-Prot for DUT Gene

Isoform 2: Nucleus.
Isoform 3: Mitochondrion.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for DUT Gene COMPARTMENTS Subcellular localization image for DUT gene
Compartment Confidence
extracellular 5
mitochondrion 5
nucleus 5
peroxisome 2
cytosol 1

Gene Ontology (GO) - Cellular Components for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 8631816
GO:0005654 nucleoplasm TAS --
GO:0005739 mitochondrion IEA --
GO:0070062 extracellular exosome IDA 20458337
genes like me logo Genes that share ontologies with DUT: view

Pathways & Interactions for DUT Gene

genes like me logo Genes that share pathways with DUT: view

UniProtKB/Swiss-Prot P33316-DUT_HUMAN

  • Pathway: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.

Gene Ontology (GO) - Biological Process for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006139 nucleobase-containing compound metabolic process TAS 1325640
GO:0006226 dUMP biosynthetic process IEA --
GO:0006260 DNA replication TAS 1325640
GO:0046081 dUTP catabolic process IBA --
GO:0046134 pyrimidine nucleoside biosynthetic process TAS --
genes like me logo Genes that share ontologies with DUT: view

No data available for SIGNOR curated interactions for DUT Gene

Drugs & Compounds for DUT Gene

(8) Drugs for DUT Gene - From: DrugBank, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
1-{(2S,5S)-4-FLUORO-5-[(TRITYLOXY)METHYL]TETRAHYDROFURAN-2-YL}PYRIMIDINE-2,4(1H,3H)-DIONE Experimental Pharma Target 0
Deoxyuridine-5'-Diphosphate Experimental Pharma Target 0
Magnesium Nutra 0

(16) Additional Compounds for DUT Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • (4-)Diphosphoric acid ion
  • (P2O74-)Diphosphate
  • Diphosphate
  • Diphosphoric acid
  • PPi
14000-31-8
Deoxyuridine triphosphate
  • 2'-Deoxyuridine 5'-triphosphate
  • 2'-Deoxyuridine-5'-triphosphorate
  • 2'-Deoxyuridine-5'-triphosphoric acid
  • 2'-Deoxyuridine-5'-triphosphoric acid = dUTP
  • Deoxy-UTP
1173-82-6
dUMP
  • 2'-Deoxy-5'-uridylate
  • 2'-Deoxy-5'-uridylic acid
  • 2'-Deoxyuridine 5'-monophosphate
  • 2'-Deoxyuridine 5'-phosphate
  • 2'-Deoxyuridylate
964-26-1
genes like me logo Genes that share compounds with DUT: view

Transcripts for DUT Gene

Unigene Clusters for DUT Gene

Deoxyuridine triphosphatase:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for DUT Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c · 2d · 2e · 2f · 2g ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b
SP1: - - - - - - - - - -
SP2: - - - - -
SP3: - - -
SP4: - -
SP5: - - - - - - - - -
SP6: - -
SP7: - - -
SP8: -
SP9: - -

Relevant External Links for DUT Gene

GeneLoc Exon Structure for
DUT
ECgene alternative splicing isoforms for
DUT

Expression for DUT Gene

mRNA expression in normal human tissues for DUT Gene

Protein differential expression in normal tissues from HIPED for DUT Gene

This gene is overexpressed in Peripheral blood mononuclear cells (10.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for DUT Gene



Protein tissue co-expression partners for DUT Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of DUT Gene:

DUT

SOURCE GeneReport for Unigene cluster for DUT Gene:

Hs.527980

mRNA Expression by UniProt/SwissProt for DUT Gene:

P33316-DUT_HUMAN
Tissue specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).
genes like me logo Genes that share expression patterns with DUT: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA differential expression in normal tissues for DUT Gene

Orthologs for DUT Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for DUT Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia DUT 34
  • 89.41 (n)
  • 90.54 (a)
DUT 35
  • 79 (a)
OneToMany
-- 35
  • 82 (a)
OneToMany
dog
(Canis familiaris)
Mammalia DUT 34
  • 84.66 (n)
  • 85.45 (a)
DUT 35
  • 77 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Dut 34
  • 81.53 (n)
  • 87.26 (a)
Dut 16
Dut 35
  • 76 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Dut 34
  • 80.86 (n)
  • 88.39 (a)
oppossum
(Monodelphis domestica)
Mammalia DUT 35
  • 83 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 35
  • 81 (a)
OneToMany
-- 35
  • 81 (a)
OneToMany
chimpanzee
(Pan troglodytes)
Mammalia DUT 35
  • 98 (a)
OneToOne
DUT 34
  • 98.94 (n)
  • 97.62 (a)
chicken
(Gallus gallus)
Aves DUT 34
  • 75.97 (n)
  • 87.01 (a)
DUT 35
  • 81 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia DUT 35
  • 65 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC101733437 34
  • 66.28 (n)
  • 73.84 (a)
Str.1294 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.7472 34
zebrafish
(Danio rerio)
Actinopterygii dut 34
  • 65.4 (n)
  • 73.42 (a)
wufa28d11 34
dut 35
  • 64 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2007 34
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010159 34
  • 65.95 (n)
  • 73.38 (a)
fruit fly
(Drosophila melanogaster)
Insecta dUTPase 34
  • 58.96 (n)
  • 65.31 (a)
dUTPase 35
  • 52 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea K07A1.2 36
  • 58 (a)
dut-1 34
  • 57.17 (n)
  • 60.61 (a)
dut-1 35
  • 21 (a)
OneToOne
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AGR249C 34
  • 54.5 (n)
  • 53.38 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes DUT1 35
  • 52 (a)
OneToOne
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.2142 34
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 67 (a)
OneToMany
CSA.6436 35
  • 62 (a)
OneToMany
Species where no ortholog for DUT was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DUT Gene

ENSEMBL:
Gene Tree for DUT (if available)
TreeFam:
Gene Tree for DUT (if available)

Paralogs for DUT Gene

genes like me logo Genes that share paralogs with DUT: view

No data available for Paralogs for DUT Gene

Variants for DUT Gene

Sequence variations from dbSNP and Humsavar for DUT Gene

SNP ID Clin Chr 15 pos Sequence Context AA Info Type
rs28381104 - 48,332,285(+) TTTCA(C/T)CCAGT nc-transcript-variant, reference, missense, utr-variant-5-prime
rs3191517 -- 48,342,064(+) GTTCC(A/C)CTGGA nc-transcript-variant, reference, missense
rs3759912 -- 48,329,639(+) tgggc(A/G)gatca upstream-variant-2KB
rs3784619 -- 48,333,741(+) TTCAC(A/G)GTGAT intron-variant
rs3784620 -- 48,340,597(+) TCAAG(C/T)CTCCC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DUT Gene

Variant ID Type Subtype PubMed ID
esv2760030 CNV loss 17122850
esv3569565 CNV gain 25503493
esv3636412 CNV gain 21293372
esv3636413 CNV loss 21293372
nsv1053885 CNV gain 25217958

Variation tolerance for DUT Gene

Residual Variation Intolerance Score: 42.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.69; 14.60% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for DUT Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
DUT

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DUT Gene

Disorders for DUT Gene

Relevant External Links for DUT

Genetic Association Database (GAD)
DUT
Human Genome Epidemiology (HuGE) Navigator
DUT
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
DUT

No disorders were found for DUT Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for DUT Gene

Publications for DUT Gene

  1. Assignment of the human dUTPase gene (DUT) to chromosome 15q15-q21. 1 by fluorescence in situ hybridization. (PMID: 9070952) Cohen D. … Pearlman R.E. (Genomics 1997) 2 3 4 22 65
  2. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. (PMID: 1325640) McIntosh E.M. … Haynes R.H. (Proc. Natl. Acad. Sci. U.S.A. 1992) 2 3 4 22 65
  3. Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase. (PMID: 17880943) Varga B. … Vertessy B.G. (FEBS Lett. 2007) 3 4 22 65
  4. The human dUTPase gene encodes both nuclear and mitochondrial isoforms. Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. (PMID: 9228092) Ladner R.D. … Caradonna S.J. (J. Biol. Chem. 1997) 3 4 22 65
  5. Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase. (PMID: 8631816) Ladner R.D. … Caradonna S.J. (J. Biol. Chem. 1996) 3 4 22 65

Products for DUT Gene

Sources for DUT Gene

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