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DUT Gene

protein-coding   GIFtS: 67
GCID: GC15P048623

Deoxyuridine Triphosphatase

(Previous name: dUTP pyrophosphatase)
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Aliases
for DUT gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Deoxyuridine Triphosphatase1 2
DUTP Pyrophosphatase1 2 3
dUTPase2 3
EC 3.6.1.233 8
Deoxyuridine 5'-Triphosphate Nucleotidohydrolase, Mitochondrial2
DUTP Nucleotidohydrolase2

External Ids:    HGNC: 30781   Entrez Gene: 18542   Ensembl: ENSG000001289517   OMIM: 6012665   UniProtKB: P333163   

Export aliases for DUT gene to outside databases

Previous GC identifers: GC15P044181 GC15P041669 GC15P046202 GC15P046339 GC15P046410 GC15P025461


Summaries
for DUT gene

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for DUT Gene:
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous,
homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes:
providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting
intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which
induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal
and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative
splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related
pseudogene is located on chromosome 19. (provided by RefSeq, Jul 2008)

GeneCards Summary for DUT Gene:
DUT (deoxyuridine triphosphatase) is a protein-coding gene. Diseases associated with DUT include laryngotracheitis, and contagious pustular dermatitis.

UniProtKB/Swiss-Prot: DUT_HUMAN, P33316
Function: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine
nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into
DNA

Gene Wiki entry for DUT Gene


Genomic Views
for DUT gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000015.10  NT_010194.18  NC_018926.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the DUT gene promoter:
         AML1a   p53   POU2F1a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): DUT promoter sequence
   Search Chromatin IP Primers for DUT

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat DUT


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q21.1   Ensembl cytogenetic band:  15q21.1   HGNC cytogenetic band: 15q21.1

DUT Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DUT gene location

GeneLoc information about chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15P048623:  view genomic region     (about GC identifiers)

Start:
 48,623,208 bp from pter      End:
 48,635,570 bp from pter
Size:
 12,363 bases      Orientation:
 plus strand

Proteins
for DUT gene

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: DUT_HUMAN, P33316 (See protein sequence)
Recommended Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial precursor  
Size: 252 amino acids; 26563 Da
Cofactor: Magnesium
Subunit: Homotrimer
Miscellaneous: Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each
substrate molecule, residues from adjacent subunits contribute to the binding interactions
Sequence caution: Sequence=AAB71393.1; Type=Frameshift; Positions=29, 47; Sequence=AAB93866.1; Type=Frameshift;
Positions=29, 47; Sequence=AAB94642.1; Type=Frameshift; Positions=29, 47;
Selected PDB 3D structures from and Proteopedia for DUT (see all 8):
1Q5H (3D)        1Q5U (3D)        2HQU (3D)        3ARA (3D)        3ARN (3D)        3EHW (3D)    
Secondary accessions: A8K650 B4DPR5 O14785 Q16708 Q16860 Q6FHN1 Q6NSA3 Q96Q81
Alternative splicing: 2 isoforms:  P33316-3   P33316-2   (Major isoform. Phosphorylated in Ser-11. Contains a phosphoserine at position 11)

Explore the universe of human proteins at neXtProt for DUT: NX_P33316

Explore proteomics data for DUT at MOPED

Post-translational modifications: 

  • Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2.
    Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated1
  • The initiator methionine is cleaved in isoform 21
  • Ubiquitination2 at Lys14, Lys63, Lys91, Lys163
  • Modification sites at PhosphoSitePlus
    • 4 DME Specific Peptides for DUT (P33316)
     GGFGSTG  GVIDEDYRGN  YGRVAPRSGLA  AAGYDLYSAYDYTIP 


    See DUT Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001020419.1  NP_001020420.1  NP_001939.1  

    ENSEMBL proteins: 
     ENSP00000453667   ENSP00000454183   ENSP00000453717   ENSP00000370376   ENSP00000452749  
     ENSP00000452814   ENSP00000405160   ENSP00000454041   ENSP00000453683  
    Reactome Protein details: P33316

    DUT Human Recombinant Protein Products:

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    Novus Biologicals DUT Proteins
    Novus Biologicals DUT Lysates
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    Cloud-Clone Corp. Proteins for DUT

    DUT Antibody Products:

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    DUT Assay Products:

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    Cloud-Clone Corp. ELISAs for DUT
    Cloud-Clone Corp. CLIAs for DUT

    Domains /
    Families
    for DUT gene

    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus    		 2 InterPro protein domains:
     IPR008180 dUTP_pyroPase
     IPR008181 dUTP_pyroPase_sf

    Graphical View of Domain Structure for InterPro Entry P33316

    ProtoNet protein and cluster: P33316

    2 Blocks protein domains:
    IPB003232 dCTP Deaminase
    IPB008180 DeoxyUTP pyrophosphatase


    UniProtKB/Swiss-Prot: DUT_HUMAN, P33316
    Similarity: Belongs to the dUTPase family


    DUT for domains           About GeneDecksing


    Function
    for DUT gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: DUT_HUMAN, P33316
    Function: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine
    nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into
    DNA
    Catalytic activity: dUTP + H(2)O = dUMP + diphosphate
    Enzyme regulation: Phosphorylation is necessary for activity
    Biophysicochemical properties: Kinetic parameters: KM=2.5 uM for dUTP; Note=for both isoform 2 and isoform 3;

         Genatlas biochemistry entry for DUT:
    dUTP pyrophosphatase,involved in DNA replication

         Enzyme Number (IUBMB): EC 3.6.1.231 2

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004170dUTP diphosphatase activity EXP--
    GO:0005515protein binding IPI16189514
    GO:0016787hydrolase activity ----
    GO:0044822poly(A) RNA binding IDA--
         
    DUT for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for DUT:
     Decreased viability  Increased cell transformation 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for DUT
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       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for DUT
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for DUT

    miRNA
    Products:            		miRTarBase miRNAs that target DUT:
    hsa-mir-1226-3p (MIRT036432), hsa-mir-26b-5p (MIRT029453), hsa-mir-193b-3p (MIRT016364), hsa-mir-744-5p (MIRT037723), hsa-mir-375 (MIRT019818), hsa-mir-34a-5p (MIRT025455)

    Block miRNA regulation of human, mouse, rat DUT using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate DUT:
    hsa-miR-374b* hsa-miR-203 hsa-miR-2115 hsa-miR-3658
    SwitchGear 3'UTR luciferase reporter plasmid: DUT 3' UTR sequence
    Inhib. RNA
    Products:            		OriGene RNAi products in human, mouse, rat for DUT
    Predesigned siRNA for gene silencing in human, mouse, rat DUT

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for DUT

    Clone
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    OriGene ORF clones in mouse, rat for DUT
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): DUT (NM_001025248)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DUT
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DUT

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for DUT 
    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DUT


    Localization
    for DUT gene

    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    DUT_HUMAN, P33316: Isoform 2: Nucleus
    DUT_HUMAN, P33316: Isoform 3: Mitochondrion
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    mitochondrion5
    nucleus5
    peroxisome2
    cytosol1

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005730NOT nucleolus IDA--
    GO:0005739mitochondrion IEA--
    GO:0070062extracellular vesicular exosome IDA--

    DUT for ontologies           About GeneDecksing


    Pathways & Interactions
    for DUT gene

    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for DUT About    
    See pathways by source

    SuperPathContained pathways About
    1Metabolism
    		Metabolism0.38
    		Metabolic pathways0.38
    2superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
    		superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis0.52
    		pyrimidine deoxyribonucleotides de novo biosynthesis0.00
    3Pyrimidine metabolism
    		Pyrimidine metabolism0.33
    		Pyrimidine biosynthesis0.00
    4Purine metabolism (REACTOME)
    		Metabolism of nucleotides0.42
    5Purine metabolism (KEGG)
    		Pyrimidine metabolism0.38

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    2 BioSystems Pathways for DUT
        superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
    pyrimidine deoxyribonucleotides de novo biosynthesis

    1 Reactome Pathway for DUT
        Pyrimidine biosynthesis


    2 Kegg Pathways  (Kegg details for DUT):
        Pyrimidine metabolism
    Metabolic pathways

    UniProtKB/Swiss-Prot: DUT_HUMAN, P33316
    Pathway: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2


    DUT for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for DUT
    Interactions:

        GeneGlobe Interaction Network for DUT

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for DUT (P333161, 2, 3 ENSP000003703764) via UniProtKB, MINT, STRING, and/or I2D (see all 78)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    NUDT18Q6ZVK81, 2, 3, ENSP000003078524EBI-353224,EBI-740486 MINT-68046 I2D: score=4 STRING: ENSP00000307852
    SPATA2Q9UM822, 3, ENSP000002894314MINT-66521 I2D: score=4 STRING: ENSP00000289431
    RHOBP627453, ENSP000002722334I2D: score=2 STRING: ENSP00000272233
    GH1P012413, ENSP000003126734I2D: score=1 STRING: ENSP00000312673
    PPARAQ078693, ENSP000002627354I2D: score=1 STRING: ENSP00000262735
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006139nucleobase-containing compound metabolic process TAS1325640
    GO:0006206pyrimidine nucleobase metabolic process TAS--
    GO:0006226dUMP biosynthetic process IEA--
    GO:0006260DNA replication TAS1325640
    GO:0044281small molecule metabolic process TAS--

    DUT for ontologies           About GeneDecksing



    Drugs & Compounds
    for DUT gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for DUT

    6 HMDB Compounds for DUT    About this table
    CompoundSynonyms CAS #PubMed Ids
    Deoxyuridine triphosphate2'-Deoxyuridine-5'-triphosphorate (see all 9)1173-82-6--
    MagnesiumMagnesium (see all 2)7439-95-4--
    Phosphoric acidacide phosphorique (FRENCH) (see all 20)7664-38-2--
    Pyrophosphate(4-)Diphosphoric acid ion (see all 10)14000-31-8--
    WaterDihydrogen oxide (see all 2)7732-18-5--
    dUMP2'-Deoxy-5'-uridylate (see all 12)964-26-1--

    2 DrugBank Compounds for DUT    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Deoxyuridine-5'-Diphosphate-- --target--17139284 17016423
    1-{(2S,5S)-4-FLUORO-5-[(TRITYLOXY)METHYL]TETRAHYDROFURAN-2-YL}PYRIMIDINE-2,4(1H,3H)-DIONE-- --target--10592235

    Selected Novoseek inferred chemical compound relationships for DUT gene (see all 18)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    dutp 91.6 149 8923782 (3), 15322254 (3), 8805593 (3), 10329142 (3) (see all 45)
    deoxyuridine 85.7 30 19052026 (2), 8595399 (2), 8013732 (2), 8221659 (2) (see all 15)
    dudp 83.8 7 17932923 (3), 11420444 (1), 8798636 (1)
    dttp 80.7 3 12369927 (1), 10329142 (1)
    2'-deoxyuridine-5-monophosphate 78.9 3 2153555 (1), 10329142 (1)
    zd9331 78 6 10952785 (3), 11487279 (1)
    thymidylate 75.1 22 15322254 (3), 10952785 (2), 10698291 (2), 18519027 (2) (see all 9)
    pyrophosphate 64.5 14 12369927 (1), 17452782 (1), 8805593 (1), 2153555 (1) (see all 9)
    fudr 61.8 14 7652165 (2), 8162567 (2), 15322254 (1), 8221659 (1) (see all 5)
    cytosine 48.7 1 1694967 (1)



    DUT for compounds           About GeneDecksing



    Transcripts
    for DUT gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

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    REFSEQ mRNAs for DUT gene (3 alternative transcripts): 
    NM_001025248.1  NM_001025249.1  NM_001948.3  

    Unigene Cluster for DUT:

    Deoxyuridine triphosphatase
    Hs.527980  [show with all ESTs]
    Unigene Representative Sequence: NM_001025248
    11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000559935 ENST00000559416 ENST00000558813 ENST00000331200(uc001zws.3 uc001zwt.3)
    ENST00000558472 ENST00000558978 ENST00000455976(uc001zww.3) ENST00000559540
    ENST00000558367 ENST00000561350 ENST00000559852
    Congresses - knowledge worth sharing:
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
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    4 qRT-PCR Assays for microRNAs that regulate DUT:
    hsa-miR-374b* hsa-miR-203 hsa-miR-2115 hsa-miR-3658
    SwitchGear 3'UTR luciferase reporter plasmid: DUT 3' UTR sequence
    Inhib. RNA
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    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DUT
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DUT
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for DUT
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat DUT
      QuantiTect SYBR Green Assays in human, mouse, rat DUT
      QuantiFast Probe-based Assays in human, mouse, rat DUT

    Additional mRNA sequence: 

    AB049113.2 AK000629.1 AK291515.1 AK298464.1 AK312122.1 BC033645.1 BC070339.1 BC110377.1 
    CR541720.1 CR541781.1 L11877.1 M89913.1 U31930.1 U62891.1 U90223.1 

    15 DOTS entries:

    DT.452923  DT.95377446  DT.100823718  DT.100823711  DT.91774168  DT.91774170  DT.100823709  DT.100823721 
    DT.218148  DT.91774169  DT.97764571  DT.95377854  DT.100035287  DT.100823717  DT.121072702 

    Selected AceView cDNA sequences (see all 417):

    BU677665 AI570675 BQ777742 CB529208 AA262687 AW160384 BI438412 AA284034 
    BG654389 CR607847 CR541720 AA830251 AA720503 CD677395 BE551158 CB133275 
    AA027178 AW162006 BM757904 BU729937 NM_001948 BI333317 CA866197 BM840182 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for DUT (see all 9)    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b · 2c · 2d · 2e · 2f · 2g ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b
    SP1:              -     -     -     -     -     -     -     -           -                             -                     
    SP2:                                            -     -     -           -                             -                     
    SP3:                                                        -           -                             -                     
    SP4:                                                        -           -                                                   
    SP5:              -     -     -     -     -     -     -     -           -                                                   


    ECgene alternative splicing isoforms for DUT

    Expression
    for DUT gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    DUT expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CAGCTCCGCT
    DUT Expression
    About this image


    DUT expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 15) fully expand
     
     Inner Cell Mass (Early Embryonic Tissues)    fully expand to see all 2 entries
             Human embryonic stem cells (family)
     
     Brain (Nervous System)    fully expand to see all 2 entries
             Cerebral Cortex
     
     Ovary (Reproductive System)
             Oviduct
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
    DUT Protein expression data from MOPED1, PaxDb2 and MaxQB3:     About this image

    DUT Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.527980

    UniProtKB/Swiss-Prot: DUT_HUMAN, P33316
    Tissue specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2
    expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with
    the cessation of nuclear DNA replication (at protein level)

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    Orthologs
    for DUT gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals and fungi.

    Orthologs for DUT gene from Selected species (see all 20)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    mouse
    (Mus musculus)    		 Mammalia Dut1 , 5 deoxyuridine triphosphatase1, 5 81.53(n)1
    87.26(a)1    		   2 (61.33 cM)5
    1100741  NM_001159646.11  NP_001153118.11 
     1252471905 
    chicken
    (Gallus gallus)    		 Aves DUT1 deoxyuridine triphosphatase 75.97(n)
    87.01(a)    		   769338  NM_001079488.1  NP_001072956.1 
    lizard
    (Anolis carolinensis)    		 Reptilia DUT6
    		 deoxyuridine triphosphatase
    		 65(a)
    		 1 ↔ 1
    		 GL343806.1(24610-36072)
    African clawed frog
    (Xenopus laevis)    		 Amphibia Xl.74722 Xenopus laevis transcribed sequence with moderate similarity more 74.45(n)    48047642 
    zebrafish
    (Danio rerio)    		 Actinopterygii wufa28d112 Transcribed sequence with moderate similarity to protein more 73.7(n)    CK354832.1 
    fruit fly
    (Drosophila melanogaster)    		 Insecta dUTPase1 Deoxyuridine triphosphatase 58.96(n)
    65.31(a)    		   34529  NM_135635.3  NP_609479.1 
    worm
    (Caenorhabditis elegans)    		 Secernentea K07A1.23
    dut-11    		 deoxyuridine 5'-triphosphate
    nucleotidohydrolase3
    dut-11    		 58(a)3
    57.17(n)1
    60.61(a)1    		   I(9593478-9595191)3
    1727941  NM_001026382.41  NP_001021553.11 
    baker's yeast
    (Saccharomyces cerevisiae)    		 Saccharomycetes DUT16
    		 deoxyuridine triphosphate diphosphatase (dUTPase);...
    		 52(a)
    		 1 ↔ 1
    		 II(722611-723054) YBR252W


    ENSEMBL Gene Tree for DUT (if available)
    TreeFam Gene Tree for DUT (if available)

    Paralogs
    for DUT gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for DUT gene

    DUT for paralogs           About GeneDecksing


    Selected Pseudogenes.org Pseudogenes for DUT (see all 9)
    PGOHUM00000262119 PGOHUM00000245069 PGOHUM00000238159 PGOHUM00000262001 PGOHUM00000246007


    Genomic Variants
    for DUT gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for DUT (see all 266)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 15 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs1812829041,2
    		--25454653(+) TAAAAC/TGAGAG 2 -- us2k10--------
    rs2006299841,2
    		--25455646(+) ATCTT-/TAAGTAC 3 -- us2k10--------
    rs2011661311,2
    		--25455648(+) CTTAG-/TACTACAA 3 -- cds10--------
    rs283810891,2
    		F--25455652(+) AGTAC-/TACAAGCA 3 -- cds15Minor allele frequency- TAC:0.02NS 188
    rs283811021,2
    		F--25457103(+) GGGGGG/CGTGGG 3 -- int1 us2k15Minor allele frequency- C:0.01NS 186
    rs1842734521,2
    		--28687828(+) AGAAGA/GTTCAA 2 -- us2k10--------
    rs37599121,2
    		C--28687972(+) tgggcA/Ggatca 2 -- us2k14Minor allele frequency- G:0.12NA WA 8
    rs1902906921,2
    		C--28688111(+) AATTGC/TTTGAA 2 -- us2k10--------
    rs80399671,2
    		A--28688189(+) aaaaaA/Caaaaa 2 -- us2k10--------
    rs80399711,2
    		A--28688195(+) aaaaaA/CTATGA 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for DUT (48623208 - 48635570 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for DUT:    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv746e1CNV Complex17122850

    Site Specific Mutation Identification with PCR Assays
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    Disorders / Diseases
    for DUT gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 601266    OMIM disorders: --

    Selected diseases for DUT (see all 25):    
    About MalaCards
    laryngotracheitis    contagious pustular dermatitis    cysticercosis    autoimmune hepatitis
    chronic fatigue syndrome    stomach cancer    herpes simplex    vaccinia
    kaposi's sarcoma    dermatitis    psoriasis    cervical cancer
    pneumonia    sarcoma    cervicitis    tuberculosis
    hiv-1    colorectal cancer    lung cancer    hepatocellular carcinoma


    DUT for disorders           About GeneDecksing

    Selected Novoseek inferred disease relationships for DUT gene (see all 11)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    visna 56.2 1 9847382 (1)
    ebv infections 38.4 4 19124728 (1)
    herpes simplex 35.2 6 2168898 (1), 8391186 (1), 9400986 (1), 10774198 (1)
    colorectal tumors 24.7 1 8162567 (1)
    neoplastic disease 18.6 1 12376965 (1)
    colon cancer 2.21 4 10910061 (2), 12374095 (1)
    breast cancer 0 1 18790783 (1)
    hiv infections 0 2 10329142 (1), 10752557 (1)
    tumors 0 9 10910061 (2), 17955371 (1), 18837522 (1), 16325515 (1) (see all 5)
    cancer 0 12 15322254 (2), 17880943 (1), 20084279 (1), 9360704 (1) (see all 10)

    Genetic Association Database (GAD): DUT
    Human Genome Epidemiology (HuGE) Navigator: DUT (3 documents)

    Export disorders for DUT gene to outside databases

    Publications
    for DUT gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for DUT gene, integrated from 10 sources (see all 149):
    (articles sorted by number of sources associating them with DUT)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. (PubMed id 1325640)1, 2, 3, 9 McIntosh E.M.... Haynes R.H. (Proc. Natl. Acad. Sci. U.S.A. 1992)
    2. Assignment of the human dUTPase gene (DUT) to chromosome 15q15-q21. 1 by fluorescence in situ hybridization. (PubMed id 9070952)1, 2, 3, 9 Cohen D....Pearlman R.E. (Genomics 1997)
    3. Identification of a consensus cyclin-dependent kinase phosphorylation site unique to the nuclear form of human deoxyuridine triphosphate nucleotidohydrolase. (PubMed id 8631817)1, 2, 9 Ladner R.D.... Caradonna S.J. (J. Biol. Chem. 1996)
    4. Human dUTP pyrophosphatase: uracil recognition by a beta hairpin and active sites formed by three separate subunits. (PubMed id 8805593)1, 2, 9 Mol C.D.... Tainer J.A. (Structure 1996)
    5. Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase. (PubMed id 8631816)1, 2, 9 Ladner R.D.... Caradonna S.J. (J. Biol. Chem. 1996)
    6. Maturation stage and proliferation-dependent expression of dUTPase in human T cells. (PubMed id 8389461)1, 2, 9 Strahler J.R.... Hanash S.M. (Proc. Natl. Acad. Sci. U.S.A. 1993)
    7. The human dUTPase gene encodes both nuclear and mitochondrial isoforms. Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. (PubMed id 9228092)1, 2, 9 Ladner R.D. and Caradonna S.J. (J. Biol. Chem. 1997)
    8. Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase. (PubMed id 17880943)1, 2, 9 Varga B.... Vertessy B.G. (FEBS Lett. 2007)
    9. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PubMed id 20877624)1, 4 Hendrickson S.L....O'Brien S.J. (PLoS ONE 2010)
    10. Polymorphisms in uracil-processing genes, but not one-carbon nutrients, are associated with altered DNA uracil concentrations in an urban Puerto Rican population. (PubMed id 19403629)1, 4 Chanson A....Mason J.B. (Am. J. Clin. Nutr. 2009)

    External Searches for DUT gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

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     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing DUT gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 1854 HGNC: 3078 AceView: DUT Ensembl:ENSG00000128951 euGenes: HUgn1854
    ECgene: DUT Kegg: 1854 H-InvDB: DUT

    Other Databases showing DUT gene

    (According to HUGE)
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    Specialized Databases showing DUT gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for DUT Pharmacogenomics, SNPs, Pathways
    NIEHS-SNPshttp://egp.gs.washington.edu/data/dut/

    Intellectual Property
    for DUT gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    TryGeneCards Plus    		Patent Information for DUT gene:
    Search GeneIP for patents involving DUT

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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