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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

DROSHA Gene

protein-coding GIFtS: 56
GCID: GC05M031401

drosha, ribonuclease type III

(Previous names: ribonuclease type III, nuclear )
(Previous symbol: RNASEN)

Explore 24 diseases affiliated with
DROSHA via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Drosha, Ribonuclease Type III¹ ²		RANSE3L² ⁵
RN3¹ ² ³ ⁵		Etohi2¹
RNASEN¹ ² ³ ⁵		ETOHI2²
RNASE3L¹ ² ³		Drosha, Double-Stranded RNA-Specific Endoribonuclease²
HSA242976¹ ²		Nuclear RNase III Drosha²
Ribonuclease Type III, Nuclear¹ ²		Putative Protein P241 Which Interacts With Transcription Factor Sp1²
Protein Drosha² ³		Putative Ribonuclease III²
P241¹		Ribonuclease 3²
RNase III² ³		Ribonuclease III, Nuclear²
EC 3.1.26.3³ ⁸		Ribonuclease III³

External Ids:	HGNC: 17904¹	Entrez Gene: 29102²	Ensembl: ENSG00000113360⁷	OMIM: 608828⁵	UniProtKB: Q9NRR4³

Export aliases for DROSHA gene to outside databases

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for DROSHA:

Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in

diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 (PubMed 12191433)).

The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the

nucleus (Lee et al., 2003 (PubMed 14508493)).(supplied by OMIM, Mar 2008)

UniProtKB/Swiss-Prot: RNC_HUMAN, Q9NRR4

Function: Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of

microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA

transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex,

DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA

junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature

miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA.

Involved in the formation of GW bodies

Gene Wiki entry for DROSHA (RNASEN)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000005.9 NC_018916.1 NT_006576.16

Regulatory elements:

Search SABiosciences Regulatory transcription factor binding sites for DROSHA
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: DROSHA promoter sequence

Search SABiosciences Chromatin IP Primers for DROSHA

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DROSHA

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5p13.3 Ensembl cytogenetic band: 5p13.3 HGNC cytogenetic band: 5q11.2

DROSHA Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DROSHA gene location

GeneLoc information about chromosome 5 GeneLoc Exon Structure

GeneLoc location for GC05M031401: view genomic region (about GC identifiers)

Start:	31,400,601 bp from pter	End:	31,532,303 bp from pter
Size:	131,703 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: RNC_HUMAN, Q9NRR4 (See protein sequence)

Recommended Name: Ribonuclease 3

Size: 1374 amino acids; 159316 Da

Cofactor: Magnesium or manganese (By similarity)

Subunit: Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA

and DGCR8. The microprocessor complex may contain multiple subunit of DGCR8 and DROSHA. Interacts with DGCR8, SP1 and

SNIP1. Interacts with SRRT/ARS2

Subcellular location: Nucleus. Nucleus, nucleolus. Note=A fraction is translocated to the nucleolus during the S phase

of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies

Sequence caution: Sequence=AAD29637.1; Type=Frameshift; Positions=775; Sequence=BAA91511.1; Type=Erroneous initiation;

Note=Translation N-terminally extended;

1 PDB 3D structure from and Proteopedia for DROSHA:

2KHX (3D)

Secondary accessions: E7EMP9 Q7Z5V2 Q86YH0 Q9NW73 Q9Y2V9 Q9Y4Y0

Alternative splicing: 4 isoforms: Q9NRR4-1 Q9NRR4-2 Q9NRR4-3 Q9NRR4-4 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for DROSHA: NX_Q9NRR4

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q9NRR4

4 DME Specific Peptides for DROSHA (Q9NRR4)

CVKGLEL

EFLGDAV

RLEFLGDA

NERLEFLGD

DROSHA Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_001093882.1 NP_037367.3

ENSEMBL proteins:

ENSP00000425979 ENSP00000424161 ENSP00000422745 ENSP00000428782 ENSP00000430921

ENSP00000339845 ENSP00000409335

Reactome Protein details: Q9NRR4
Human Recombinant Protein Products for DROSHA:

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	Novus Biologicals DROSHA Protein
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for DROSHA

Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005622	intracellular	--	--
GO:0005654	nucleoplasm	TAS	--
GO:0005730	nucleolus	IEA	--

DROSHA for ontologies About GeneDecksing

DROSHA Antibody Products:

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	Novus Biologicals DROSHA Antibodies
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	ThermoFisher Antibodies for DROSHA

Assay Products for DROSHA:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for DROSHA
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for DROSHA

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

DROSHA for domains About GeneDecksing

4 InterPro domains/families:

IPR001159 Ds-RNA-bd

IPR011907 RNase_III

IPR000999 RNase_III_dom

IPR014720 dsRNA-bd-like_dom

Graphical View of Domain Structure for InterPro Entry Q9NRR4

ProtoNet protein and cluster: Q9NRR4

2 Blocks protein families:

IPB000999 Ribonuclease III family

IPB001159 Double-stranded RNA binding (DsRBD) domain

UniProtKB/Swiss-Prot: RNC_HUMAN, Q9NRR4

Domain: The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2

cleaves the 5'strand of pri-miRNAs, independently of each other

Similarity: Contains 1 DRBM (double-stranded RNA-binding) domain

Similarity: Contains 2 RNase III domains

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: RNC_HUMAN, Q9NRR4

Function: Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of

microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA

transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex,

DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA

junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature

miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA.

Involved in the formation of GW bodies

Catalytic activity: Endonucleolytic cleavage to 5'-phosphomonoester

Enzyme Number (IUBMB): EC 3.1.26.3¹ ²

Gene Ontology (GO): 5/6 molecular function terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003723	RNA binding	--	--
GO:0003725	double-stranded RNA binding	IEA	--
GO:0004521	endoribonuclease activity	--	--
GO:0004525	ribonuclease III activity	TAS	--
GO:0005515	protein binding	IPI	15574589

DROSHA for ontologies About GeneDecksing

Phenotypes:
12 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Drosha):

adipose tissue	cardiovascular system	cellular	digestive/alimentary	growth/size
hematopoietic system	immune system	integument	liver/biliary system	mortality/aging
muscle	respiratory system

DROSHA for phenotypes About GeneDecksing

Animal Models:
Mouse knock-out Drosha^tm1.1Litt for DROSHA

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for DROSHA

miRNA Products:		OriGene 3'-UTR Clone (see all 2): DROSHA Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat DROSHA
		8/18 QIAGEN miScript miRNA Assays for microRNAs that regulate DROSHA (see all 18):
		hsa-miR-4288 hsa-miR-128 hsa-miR-632 hsa-miR-1205 hsa-miR-15b* hsa-miR-205* hsa-miR-1231 hsa-miR-4289

		SwitchGear 3'UTR luciferase reporter plasmid: DROSHA 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for DROSHA (see all 4) OriGene shRNA RFP: DROSHA OriGene siRNA: DROSHA
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Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DROSHA (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DROSHA (see all 2) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): DROSHA (NM_013235)
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Cell Line Products:		GenScript Custom overexpressing Cell Line Services for DROSHA
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		Sirion Biotech Customized stable knockdown cell line services for DROSHA Sirion Biotech Customized inducible knockdown cell line services for DROSHA Sirion Biotech Customized stable overexpressing cell line services for DROSHA Sirion Biotech Customized inducible overexpressing cell line services for DROSHA

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DROSHA

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Regulatory RNA pathways

Regulatory RNA pathways

1.00

MicroRNA (miRNA) Biogenesis

0.88

Direct p53 effectors

Direct p53 effectors

1.00

Ribosome biogenesis in eukaryotes

Ribosome biogenesis in eukaryotes

1.00

Translational Control

Translational Control

1.00

Generic Transcription Pathway

Gene Expression

0.46

Pathway sources
See GeneCards unified pathways
Show all pathways

1 Cell Signaling Technology (CST) Pathway for DROSHA

Translational Control

1 BioSystems Pathway for DROSHA

Direct p53 effectors

3 Reactome Pathways for DROSHA

	MicroRNA (miRNA) Biogenesis
	Regulatory RNA pathways
	Gene Expression

1 Kegg Pathway (Kegg details for DROSHA):

Ribosome biogenesis in eukaryotes

DROSHA for pathways About GeneDecksing

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for DROSHA

STRING Interaction Network Preview (showing 5 interactants - click image to see 23)

5/28 Interacting proteins for DROSHA (Q9NRR4^{1, 2, 3} ENSP00000339845⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 28)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
DGCR8	Q8WYQ5¹^,²^,³, ENSP00000263209⁴	EBI-528367,EBI-528411 MINT-8329598 I2D: score=4 STRING: ENSP00000263209
ZNF512B	Q96KM6²^,³, ENSP00000217130⁴	MINT-60789 I2D: score=2 STRING: ENSP00000217130
SNIP1	Q8TAD8³, ENSP00000296215⁴	I2D: score=1 STRING: ENSP00000296215
SP1	P08047³, ENSP00000329357⁴	I2D: score=2 STRING: ENSP00000329357
BCL6	P41182³, ENSP00000232014⁴	I2D: score=2 STRING: ENSP00000232014

About this table

Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006396	RNA processing	--	--
GO:0010467	gene expression	TAS	--
GO:0010586	miRNA metabolic process	IEA	--
GO:0016075	rRNA catabolic process	IEA	--
GO:0031047	gene silencing by RNA	--	--

DROSHA for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

DROSHA for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for DROSHA
3 Novoseek chemical compound relationships for DROSHA gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
polynucleotide	61.3	7	11726520 (4), 1628624 (2), 8901510 (1)
leucine	0	1	17017124 (1)
adenylate	0	1	15100988 (1)

Search CenterWatch for drugs/clinical trials and news about DROSHA / RNC

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for DROSHA gene (2 alternative transcripts):

NM_001100412.1 NM_013235.4

Unigene Cluster for DROSHA:

Drosha, ribonuclease type III

Hs.97997 [show with all ESTs]

Unigene Representative Sequence: NM_013235

18/21 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 21):

ENST00000511367(uc003jhh.2 uc003jhg.2) ENST00000513349(uc003jhi.2 uc010iui.1)

ENST00000514927 ENST00000511778 ENST00000512166 ENST00000509067 ENST00000504133

ENST00000510178 ENST00000505601 ENST00000507174 ENST00000509608 ENST00000512124

ENST00000511803 ENST00000510375 ENST00000512885 ENST00000512076 ENST00000512302

ENST00000507438

miRNA Products:		OriGene 3'-UTR Clone (see all 2): DROSHA Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat DROSHA
		8/18 QIAGEN miScript miRNA Assays for microRNAs that regulate DROSHA (see all 18):
		hsa-miR-4288 hsa-miR-128 hsa-miR-632 hsa-miR-1205 hsa-miR-15b* hsa-miR-205* hsa-miR-1231 hsa-miR-4289

		SwitchGear 3'UTR luciferase reporter plasmid: DROSHA 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
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		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DROSHA
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DROSHA

Additional cDNA sequence:

AF116910.1 AF189011.1 AJ242976.1 AK001121.1 AK026563.1 AK308093.1 BC024261.1 BC041162.1

BC054003.1 BX647724.1

22 DOTS entries:

DT.441061 DT.75155929 DT.101983341 DT.95135231 DT.95135237 DT.91902617 DT.75145749 DT.91931526

DT.120873851 DT.91755996 DT.95331086 DT.99956997 DT.120873889 DT.120873903 DT.40211533 DT.431844

DT.86852147 DT.91999881 DT.101983342 DT.120873879 DT.40119217 DT.91905742

24/180 AceView cDNA sequences (see all 180):

CR613595 BX090869 BP349758 BU155281 AU120566 AA917625 BC024261 BP363363

BE385337 AA460045 AW874309 AW438881 AU142521 AF116910 BM698705 AV709821

AA749416 AI972095 CR607257 BX647724 BC054003 AI864626 BC041162 F19104

GeneLoc Exon Structure

5/15 Alternative Splicing Database (ASD) splice patterns (SP) for DROSHA (see all 15) About this scheme

ExUns:	1a	·	1b	·	1c	^	2a	·	2b	^	3	^	4	^	5	^	6a	·	6b	^	7	^	8	^	9	^	10	^	11	^	12	^	13	^	14	^	15	^	16	^	17	^	18	^	19	^	20a	·	20b	^	21	^
SP1:							-																-														-														-
SP2:							-		-																												-														-
SP3:							-		-														-														-														-
SP4:																					-		-
SP5:

ExUns:	22a	·	22b	^	23a	·	23b	^	24	^	25a	·	25b	^	26	^	27a	·	27b	^	28	^	29	^	30	^	31	^	32a	·	32b	^	33a	·	33b	·	33c	^	34a	·	34b	^	35a	·	35b	^	36a	·	36b	^	37a	·
SP1:	-						-										-												-														-						-		-
SP2:	-						-										-												-														-						-		-
SP3:	-						-										-												-
SP4:
SP5:																													-

ExUns:	37b	^	38	^	39a	·	39b
SP1:
SP2:
SP3:
SP4:
SP5:

ECgene alternative splicing isoforms for DROSHA

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

DROSHA expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CAAGTGTGGA

About this image
See DROSHA Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for DROSHA

SOURCE GeneReport for Unigene cluster: Hs.97997

UniProtKB/Swiss-Prot: RNC_HUMAN, Q9NRR4

Tissue specificity: Ubiquitous

SABiosciences Custom PCR Arrays for DROSHA

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DROSHA Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat DROSHA
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DROSHA
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DROSHA

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DROSHA

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the last universal common ancestor (LUCA).

Orthologs for DROSHA gene from 8/28 species (see all 28) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Drosha^{1 , 5}	drosha, ribonuclease type III^{1, 5}	87.8(n)¹ 95.77(a)¹		15 (5.99 cM)⁵ 14000¹ NM_001130149.1¹ NP_001123621.1¹ 12824863⁵
chicken (Gallus gallus)	Aves	DROSHA¹	drosha, ribonuclease type III	79.87(n) 88.48(a)		420911 NM_001006379.1 NP_001006379.1
lizard (Anolis carolinensis)	Reptilia	DROSHA⁶	--	83(a)	1 ↔ 1	GL343408.1(243814-379744)
African clawed frog (Xenopus laevis)	Amphibia	Xl.15543²	Xenopus laevis transcribed sequence with moderate similarity more	82.56(n)		BJ094119.1
zebrafish (Danio rerio)	Actinopterygii	Dr.11510²	Transcribed sequence with weak similarity to protein more	76.41(n)		BG303805.1
fruit fly (Drosophila melanogaster)	Insecta	drosha¹	CG8730-PA	53.63(n) 51.64(a)		35747 NM_058088.3 NP_477436.1
worm (Caenorhabditis elegans)	Secernentea	drsh-1¹	Protein DRSH-1	45.52(n) 32.86(a)		172830 NM_001128988.1 NP_001122460.1
E. coli (Escherichia coli)	Gamma proteobacteria	rnc⁶	RNase III	30(a)	1 ↔ 1	Chromosome(2701405-2702085)

ENSEMBL Gene Tree for DROSHA (if available)
TreeFam Gene Tree for DROSHA (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for DROSHA gene

FIP1L1²

DROSHA for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 5 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs142015128^1,2		--	31400103(+)	`CGCCCA/GCCACC`	2	--	ds500¹		0	--	--	--	--
rs183266298^1,2		--	31400112(+)	`CCATGC/TCTGGG`	2	--	ds500¹		0	--	--	--	--
rs188141441^1,2		--	31400301(+)	`TTATAG/TAAGAA`	2	--	ds500¹		0	--	--	--	--
rs116701218^1,2	F	--	31400374(+)	`TTAGGG/AAGAAC`	2	--	ds500¹		1		WA	118
rs193078280^1,2		--	31400817(+)	`TATTGA/GGAGGT`	2	--	ut3¹		0	--	--	--	--
rs184640602^1,2		--	31400998(+)	`GCCAAA/TTAATT`	2	--	ut3¹		0	--	--	--	--
rs642321^1,2	C,F,O,A,H	--	31401003(+)	`TTAATC/TGTTAA`	2	--	ut3¹		37		EA NA MN NS WA CSA	5602
rs116086102^1,2	C,F	--	31401147(+)	`GATGTT/CACTAG`	2	--	ut3¹		1		WA	118
rs114594866^1,2	C,F	--	31401250(+)	`TATTCC/TACATA`	2	--	ut3¹		1		WA	118
rs150603393^1,2		--	31401392(+)	`CAGATA/CATTAA`	2	--	ut3¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for DROSHA (31400601 - 31532303 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for DROSHA: --
Human Gene Mutation Database (HGMD): DROSHA

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DROSHA

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

DROSHA for disorders           About GeneDecksing

OMIM gene information: 608828    OMIM disorders: --

20/24 diseases for DROSHA (see all 24):    About MalaCards

muscle hypertrophy esophageal squamous cell carcinoma squamous cell carcinoma digeorge syndrome

renal cell carcinoma esophagitis hepatitis b skin cancer

endometrial cancer esophageal cancer cervical cancer cervicitis

colon cancer ovarian cancer lung cancer carcinoma

hepatitis pneumonia neuroblastoma schizophrenia

Export disorders for DROSHA gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for DROSHA gene, integrated from 9 sources (see all 162):
(articles sorted by number of sources associating them with DROSHA)

Utopia: connect your pdf to the dynamic
world of online information

Human RNase III is a 160-kDa protein involved in preribosomal RNA processing. (PubMed id 10948199)^{1, 2, 3, 9} Wu H.... Crooke S.T. (2000)
A novel type of RNase III family proteins in eukaryotes. (PubMed id 10713462)^{1, 3, 9} Filippov V....Gill S.S. (2000)
The Drosha-DGCR8 complex in primary microRNA processing. (PubMed id 15574589)^{1, 2, 9} Han J.... Kim V.N. (2004)
The nuclear RNase III Drosha initiates microRNA processing. (PubMed id 14508493)^{1, 2, 9} Lee Y.... Kim V.N. (2003)
The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis. (PubMed id 15589161)^{1, 2, 9} Landthaler M.... Tuschl T. (2004)
The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis. (PubMed id 18632581)^{1, 2} Yu B.... Chen X. (2008)
Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha. (PubMed id 15565168)^{1, 2} Zeng Y....Cullen B.R. (2005)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
The Microprocessor complex mediates the genesis of microRNAs. (PubMed id 15531877)^{1, 2} Gregory R.I....Shiekhattar R. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 29102	HGNC: 17904	AceView: RNASE3L	Ensembl:ENSG00000113360	euGenes: HUgn29102
ECgene: DROSHA	Kegg: 29102	H-InvDB: DROSHA

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for DROSHA	Pharmacogenomics, SNPs, Pathways
Protein Spotlight	http://web.expasy.org/spotlight/back_issues/sptlt087.shtml

Intellectual Property for DROSHA gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for DROSHA gene:
Search GeneIP for patents involving DROSHA

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
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drosha, ribonuclease type III

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DROSHA (Gene Symbol)
Drosha, Ribonuclease Type III
RN3
RNASEN
RNASE3L
HSA242976
Ribonuclease Type III, Nuclear
Protein Drosha
P241
RNase III
EC 3.1.26.3
RANSE3L
Etohi2
ETOHI2
Drosha, Double-Stranded RNA-Specific Endoribonuclease
Nuclear RNase III Drosha
Putative Protein P241 Which Interacts With Transcription Factor Sp1
Putative Ribonuclease III
Ribonuclease 3
Ribonuclease III, Nuclear
Ribonuclease III

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DROSHA Gene

drosha, ribonuclease type III

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