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Aliases for DPH5 Gene

Aliases for DPH5 Gene

  • Diphthamide Biosynthesis 5 2 3 5
  • Diphthamide Biosynthesis Methyltransferase 3 4
  • DPH5 Homolog (S. Cerevisiae) 2
  • Diphthine Synthase 3
  • Protein X 0011 3
  • DPH5 Homolog 3
  • EC 4
  • EC 63
  • HSPC143 3
  • AD-018 3
  • CGI-30 3
  • NPD015 3

External Ids for DPH5 Gene

Previous GeneCards Identifiers for DPH5 Gene

  • GC01M101168
  • GC01M101227
  • GC01M101455
  • GC01M099580

Summaries for DPH5 Gene

Entrez Gene Summary for DPH5 Gene

  • This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for DPH5 Gene

DPH5 (Diphthamide Biosynthesis 5) is a Protein Coding gene. Among its related pathways are Gamma carboxylation, hypusine formation and arylsulfatase activation and Transport to the Golgi and subsequent modification. GO annotations related to this gene include methyltransferase activity and diphthine synthase activity.

UniProtKB/Swiss-Prot for DPH5 Gene

  • S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.

Gene Wiki entry for DPH5 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DPH5 Gene

Genomics for DPH5 Gene

Regulatory Elements for DPH5 Gene

Enhancers for DPH5 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around DPH5 on UCSC Golden Path with GeneCards custom track

Promoters for DPH5 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around DPH5 on UCSC Golden Path with GeneCards custom track

Genomic Location for DPH5 Gene

100,989,623 bp from pter
101,026,088 bp from pter
36,466 bases
Minus strand

Genomic View for DPH5 Gene

Genes around DPH5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DPH5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DPH5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DPH5 Gene

Proteins for DPH5 Gene

  • Protein details for DPH5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Diphthine methyl ester synthase
    Protein Accession:
    Secondary Accessions:
    • A8JZY6
    • D3DT62
    • Q9P017
    • Q9P0I4
    • Q9Y319

    Protein attributes for DPH5 Gene

    285 amino acids
    Molecular mass:
    31651 Da
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for DPH5 Gene

neXtProt entry for DPH5 Gene

Proteomics data for DPH5 Gene at MOPED

Selected DME Specific Peptides for DPH5 Gene

Post-translational modifications for DPH5 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for DPH5 Gene

Domains & Families for DPH5 Gene

Protein Domains for DPH5 Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the diphthine synthase family.
  • Belongs to the diphthine synthase family.
genes like me logo Genes that share domains with DPH5: view

No data available for Gene Families for DPH5 Gene

Function for DPH5 Gene

Molecular function for DPH5 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
4 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2].
UniProtKB/Swiss-Prot Function:
S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.

Enzyme Numbers (IUBMB) for DPH5 Gene

Phenotypes for DPH5 Gene

GenomeRNAi human phenotypes for DPH5:
genes like me logo Genes that share phenotypes with DPH5: view

Animal Model Products

miRNA for DPH5 Gene

miRTarBase miRNAs that target DPH5

No data available for Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for DPH5 Gene

Localization for DPH5 Gene

Subcellular locations from

Jensen Localization Image for DPH5 Gene COMPARTMENTS Subcellular localization image for DPH5 gene
Compartment Confidence
cytosol 5
nucleus 2

No data available for Subcellular locations from UniProtKB/Swiss-Prot and Gene Ontology (GO) - Cellular Components for DPH5 Gene

Pathways & Interactions for DPH5 Gene

genes like me logo Genes that share pathways with DPH5: view

UniProtKB/Swiss-Prot Q9H2P9-DPH5_HUMAN

  • Pathway: Protein modification; peptidyl-diphthamide biosynthesis.

Gene Ontology (GO) - Biological Process for DPH5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0043687 post-translational protein modification TAS --
GO:0044267 cellular protein metabolic process TAS --
genes like me logo Genes that share ontologies with DPH5: view

No data available for SIGNOR curated interactions for DPH5 Gene

Drugs & Compounds for DPH5 Gene

(2) Drugs for DPH5 Gene - From: DrugBank and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
S-Adenosylmethionine Approved Nutra 0
s-adenosylhomocysteine Experimental Pharma Target 0

(3) Additional Compounds for DPH5 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • 1-Carboxy-3-(L-histidin-2-yl)-N-methylpropan-1-aminium
  • 2-[3-Carboxy-3-(methylammonio)propyl]-His
  • 2-[3-Carboxy-3-(methylammonio)propyl]-histidine
  • [3-[[4-(2-amino-2-carboxy-ethyl)-1H-imidazol-2-yl]]-1-carboxy- propyl]-methyl-ammonium
  • 2-(3-Amino-3-carboxypropyl)-L-histidine
  • 2-Amino-4-[[4-(2-amino-2-carboxy-ethyl)-1H-imidazol-2-yl]] butanoate
  • 2-Amino-4-[[4-(2-amino-2-carboxy-ethyl)-1H-imidazol-2-yl]] butanoic acid
genes like me logo Genes that share compounds with DPH5: view

Transcripts for DPH5 Gene

Unigene Clusters for DPH5 Gene

DPH5 homolog (S. cerevisiae):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for DPH5 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c · 2d · 2e · 2f ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11
SP1: - - - - -
SP2: - - - - - -
SP3: - - - - - -
SP4: - - - - - - -
SP5: - - - - - - -
SP6: - - - - - - - -
SP7: - - - - -
SP8: - -
SP9: - - - -
SP10: - - - - - - - -
SP11: - - - - - - -
SP12: - -
SP13: - -

Relevant External Links for DPH5 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for DPH5 Gene

mRNA expression in normal human tissues for DPH5 Gene

Protein differential expression in normal tissues from HIPED for DPH5 Gene

This gene is overexpressed in Monocytes (10.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for DPH5 Gene

SOURCE GeneReport for Unigene cluster for DPH5 Gene Hs.440776

genes like me logo Genes that share expression patterns with DPH5: view

Protein tissue co-expression partners for DPH5 Gene

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for DPH5 Gene

Orthologs for DPH5 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for DPH5 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia DPH5 35
  • 92.16 (n)
  • 94.39 (a)
DPH5 36
  • 94 (a)
(Canis familiaris)
Mammalia DPH5 35
  • 91.64 (n)
  • 92.11 (a)
DPH5 36
  • 91 (a)
(Mus musculus)
Mammalia Dph5 35
  • 87.19 (n)
  • 92.17 (a)
Dph5 16
Dph5 36
  • 92 (a)
(Pan troglodytes)
Mammalia DPH5 35
  • 99.3 (n)
  • 100 (a)
DPH5 36
  • 100 (a)
(Rattus norvegicus)
Mammalia Dph5 35
  • 88.14 (n)
  • 92.17 (a)
(Monodelphis domestica)
Mammalia DPH5 36
  • 79 (a)
(Ornithorhynchus anatinus)
Mammalia DPH5 36
  • 83 (a)
(Gallus gallus)
Aves DPH5 35
  • 76.16 (n)
  • 80.78 (a)
gga-mir-1610 36
  • 79 (a)
(Anolis carolinensis)
Reptilia DPH5 36
  • 77 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia dph5 35
  • 68.8 (n)
  • 73.31 (a)
Str.7197 35
African clawed frog
(Xenopus laevis)
Amphibia cgi-30-prov 35
(Danio rerio)
Actinopterygii dph5 35
  • 69.96 (n)
  • 78.75 (a)
dph5 36
  • 74 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.13925 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP004412 35
  • 59.78 (n)
  • 63.84 (a)
fruit fly
(Drosophila melanogaster)
Insecta Dph5 35
  • 59.9 (n)
  • 63.47 (a)
Dph5 36
  • 61 (a)
(Caenorhabditis elegans)
Secernentea B0491.7 35
  • 61.09 (n)
  • 63.94 (a)
B0491.7 36
  • 62 (a)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AFR123W 35
  • 55.56 (n)
  • 61.05 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0F22836g 35
  • 57.52 (n)
  • 61.28 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes DPH5 35
  • 58.43 (n)
  • 58.43 (a)
DPH5 36
  • 52 (a)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT4G31790 35
  • 63.77 (n)
  • 61.13 (a)
(Oryza sativa)
Liliopsida Os03g0355800 35
  • 58.8 (n)
  • 60.3 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.14906 35
bread mold
(Neurospora crassa)
Ascomycetes NCU07266 35
  • 55.85 (n)
  • 60.82 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes dph5 35
  • 58.58 (n)
  • 60.45 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 65 (a)
Species with no ortholog for DPH5:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DPH5 Gene

Gene Tree for DPH5 (if available)
Gene Tree for DPH5 (if available)

Paralogs for DPH5 Gene

No data available for Paralogs for DPH5 Gene

Variants for DPH5 Gene

Sequence variations from dbSNP and Humsavar for DPH5 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs1188736 -- 101,020,021(-) TGCTA(A/G)AACCA intron-variant
rs3835395 -- 101,022,560(-) TGATC(-/CTTC)ATTCT intron-variant
rs2893311 -- 101,015,203(+) TATCT(A/G)GGGCA intron-variant
rs3887102 -- 101,015,221(-) atttt(A/G)tgagg intron-variant
rs4000624 -- 101,015,235(+) TTTCT(-/TAGA)TAGAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DPH5 Gene

Variant ID Type Subtype PubMed ID
nsv522064 CNV Gain 19592680
esv2668878 CNV Deletion 23128226

Variation tolerance for DPH5 Gene

Residual Variation Intolerance Score: 48.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.00; 36.92% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Relevant External Links for DPH5 Gene

Disorders for DPH5 Gene

Relevant External Links for DPH5

Genetic Association Database (GAD)
Atlas of Genetics and Cytogenetics in Oncology and Haematology:

No disorders were found for DPH5 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for DPH5 Gene

Publications for DPH5 Gene

  1. A modified form of diphthamide causes immunotoxin resistance in a lymphoma cell line with a deletion of the WDR85 gene. (PMID: 23486472) Wei H. … Pastan I. (J. Biol. Chem. 2013) 2 3 4 67
  2. Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. (PMID: 15485916) Liu S. … Leppla S.H. (Mol. Cell. Biol. 2004) 2 3 23
  3. Panorama of ancient metazoan macromolecular complexes. (PMID: 26344197) Wan C. … Emili A. (Nature 2015) 3
  4. Drug-gene interactions and the search for missing heritability: a cross-sectional pharmacogenomics study of the QT interval. (PMID: 23459443) Avery C.L. … Psaty B.M. (Pharmacogenomics J. 2013) 3
  5. Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. (PMID: 22658674) Castello A. … Hentze M.W. (Cell 2012) 3

Products for DPH5 Gene

Sources for DPH5 Gene