DPH3 Gene
protein-coding GIFtS: 45
GCID: GC03M016298
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DPH3, KTI11 homolog (S. cerevisiae)(Previous names: zinc finger, CSL-type containing 2, DPH3 homolog (KTI11,...)
(Previous symbol: ZCSL2)
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Aliases
for DPH3 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| DPH3, KTI11 Homolog (S. Cerevisiae)1 2 | | CSL-Type Zinc Finger-Containing Protein 22 3 | | ZCSL21 2 3 5 | | MGC201971 | | DESR11 2 3 | | Deafness Locus Putative Guanine Nucleotide Exchange Factor Interacting Protein12 | | DELGIP11 2 5 | | Del-GEF Interacting Protein2 | | KTI111 2 5 | | Diphtheria Toxin And Pseudomonas Exotoxin A Sensitivity Required Gene 12 | | DELGIP1 2 | | DPH3 Homolog2 | | DPH3A1 2 | | DPH3A, KTI11 Homolog A2 | | DPH3 Homolog (KTI11, S. Cerevisiae)1 2 | | Zinc Finger, CSL Domain Containing 22 | | Zinc Finger, CSL-Type Containing 21 2 | | DelGIP13 | | DelGEF-Interacting Protein 12 3 | | |
Export aliases for DPH3 gene to outside databasesPrevious GC identifer: GC03M016277 |
Summaries
for DPH3 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for DPH3:
This gene encodes a CSL zinc finger-containing protein that is required for dipthamide biosynthesis. The encodedprotein is necessary for the initial step in the modification of a histidine residue in elongation factor-2 todiphthamide. This modified residue is a target for ADP ribosylation by the bacterial toxins diphtheria toxin andPseudomonas exotoxin A. Alternative splicing results in multiple transcript variants that encode the same isoform.(provided by RefSeq, Feb 2009)
UniProtKB/Swiss-Prot: DPH3_HUMAN, Q96FX2Function: Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidinewhich occurs in elongation factor 2 and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A(By similarity)Function: Down-regulation increases extracellular release of proteoglycans, indicating a possible role in the secretionprocess. Stimulates binding of GNEFR to SEC5
|
Genomic Views
for DPH3 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000003.11 NC_018914.1 NT_022517.18
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the DPH3 gene promoter:
Pbx1a HTF AREB6 CREB E2F E2F-1 deltaCREB FOXJ2 (long isoform) FOXJ2 ATF6
Other transcription factors
Search SABiosciences Chromatin IP Primers for DPH3
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DPH3 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 3p25.1 Ensembl cytogenetic band: 3p25.1 HGNC cytogenetic band: 3p25.1DPH3 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 3 GeneLoc Exon Structure GeneLoc location for GC03M016298: view genomic region
(about GC identifiers)
Start:
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16,298,568 bp from pter |
End:
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16,306,496 bp from pter |
Size:
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7,929 bases |
Orientation:
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minus strand |
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Proteins
for DPH3 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: DPH3_HUMAN, Q96FX2 (See
protein sequence)Recommended Name: DPH3 homolog Size: 82 amino acids; 9240 Da
Subcellular location: Cytoplasm. Nucleus
1 PDB 3D structure from  and Proteopedia  for DPH3:2JR7 (3D)
Alternative splicing: 2 isoforms: Q96FX2-1 Q96FX2-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for DPH3: NX_Q96FX2
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q96FX2
DPH3 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001040899.1 NP_996662.1
ENSEMBL proteins: ENSP00000419599 ENSP00000373285
Human Recombinant Protein Products:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
DPH3 for ontologies About GeneDecksing
DPH3 Antibody Products:
Assay Products for DPH3: |
Protein
Domains /
Families
for DPH3 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
DPH3 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q96FX2ProtoNet protein and cluster: Q96FX2 UniProtKB/Swiss-Prot: DPH3_HUMAN, Q96FX2Similarity: Belongs to the DPH3 familySimilarity: Contains 1 DPH-type zinc finger |
Function
for DPH3 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: DPH3_HUMAN, Q96FX2Function: Essential for the first step in the synthesis of diphthamide, a post-translational modification of histidinewhich occurs in elongation factor 2 and which can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A(By similarity)Function: Down-regulation increases extracellular release of proteoglycans, indicating a possible role in the secretionprocess. Stimulates binding of GNEFR to SEC5
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DPH3 (see all 6)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DPH3 (see all 3)
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DPH3 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table
DPH3 for ontologies About GeneDecksing
Animal Models:
Mouse knock-out Dph3tm1Lepp for DPH3
5 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Dph3):
DPH3 for phenotypes About GeneDecksing
|
Pathways & Interactions
for DPH3 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for DPH3
STRING Interaction
Network Preview (showing 5 interactants - click image to see 17)
5/17 Interacting proteins for DPH3 (Q96FX21, 3 ENSP000004195994) via UniProtKB, MINT, STRING, and/or I2D (see all 17)About this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0017183 | peptidyl-diphthamide biosynthetic process from peptidyl-histidine |
ISS | -- | | GO:0050709 | negative regulation of protein secretion |
IDA | 14980502 | | GO:0051099 | positive regulation of binding |
IDA | 14980502 |
DPH3 for ontologies About GeneDecksing
|
Drugs & Compounds
for DPH3 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for DPH3
Search CenterWatch for drugs/clinical trials and news about DPH3
|
Transcripts
for DPH3 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for DPH3 gene (2 alternative transcripts): NM_001047434.2 NM_206831.2 Unigene Cluster for DPH3: DPH3, KTI11 homolog (S. cerevisiae) Hs.388087 [show with all ESTs]Unigene Representative Sequence: NM_2068314 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000488423(uc003cat.4 uc003cau.3 uc003cav.3) ENST00000383775
ENST00000285082 ENST00000462982
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DPH3 (see all 6)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DPH3 (see all 3)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): DPH3 (NM_206831) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for DPH3 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DPH3 |
Additional cDNA sequence: AK022970.1 AK023203.1 AK092040.1 AK311618.1 BC010181.2 BC073931.1 BC094863.1 6 DOTS entries: DT.95262116 DT.100782472 DT.95257646 DT.92420590 DT.100734000 DT.100782473 24/194 AceView cDNA sequences (see all 194): AA777638 F12397 AA831174 BC010181 BM717191 BF063630 AA721309 BM452855 BM549276 N80873 NM_206831 AA502392 AW661665 AA865334 BM834930 N33756 C04626 AI097226 BQ213445 AI249053 BM504521 BM550423 AI122599 CD370780
GeneLoc Exon Structure
5 Alternative Splicing Database (ASD) splice patterns (SP) for DPH3 About this scheme
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | · | 1e | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | · | 4c | · | 4d | · | 4e |
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| SP1: | | | | | | | | | - | | - | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | - | | - | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | - | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | - | | - | | - | | | | | | | | | | | |
ECgene alternative splicing isoforms for DPH3
|
Expression
for DPH3 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| DPH3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See DPH3 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for DPH3
SOURCE GeneReport for Unigene cluster: Hs.388087
UniProtKB/Swiss-Prot: DPH3_HUMAN, Q96FX2Tissue specificity: Widely expressed with highest levels in small intestine, spleen, thymus, heart, liver and lung
SABiosciences Custom PCR Arrays for DPH3
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DPH3
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat DPH3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DPH3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DPH3 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DPH3 |
Orthologs
for DPH3 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for DPH3 gene from 7/24 species (see all 24) About this table
ENSEMBL Gene Tree for DPH3 (if available)
TreeFam Gene Tree for DPH3 (if available) |
Paralogs
for DPH3 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for DPH3 gene
2 SIMAP similar genes for DPH3 using alignment to 1 protein entry: DPH3_HUMAN:DPH3P1 DNAJC24
DPH3 for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for DPH3
PGOHUM00000242102
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Genomic Variants
for DPH3 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 3 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for DPH3 (16298568 - 16306496 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for DPH3: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DPH3 |
|
Disorders
/ Diseases
for DPH3 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
DPH3 for disorders About GeneDecksing
OMIM gene information: 608959
OMIM disorders: --
2  diseases for DPH3: About MalaCardsdiphtheria malaria
1 disease from the University of Copenhagen DISEASES database for DPH3:Diphtheria Export disorders for DPH3 gene to outside databases
|
Publications
for DPH3 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for DPH3 gene integrated from 9 sources:
(articles sorted by number of sources associating them with DPH3) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Characterisation of an evolutionary conserved protein interacting with the putative guanine nucleotide exchange factor DelGEF and modulating secretion. (PubMed id 14980502)1, 2, 3, 9 Sjoelinder M.... Ponstingl H. (2004)
- Retroviral insertional mutagenesis identifies a small protein required for synthesis of diphthamide, the target of bacterial ADP- ribosylating toxins. (PubMed id 14527407)1, 2, 3 Liu S. and Leppla S.H. (2003)
- Solution structure of human DESR1, a CSL zinc-binding protein. (PubMed id 18214955)1, 2 Wu F....Shi Y. (2008)
- Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. (PubMed id 15485916)1, 3 Liu S....Leppla S.H. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
- Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
|
External Searches for DPH3 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing DPH3 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing DPH3 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing DPH3 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for DPH3 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for DPH3 gene:
Search GeneIP for patents involving DPH3
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for DPH3 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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variant library, vector shuttling | | | OriGene Custom Antibody Services for DPH3 | | OriGene Custom Protein Services for DPH3 | | | OriGene Custom Immunoassay Development | | |
| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat DPH3 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DPH3 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DPH3 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat DPH3 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DPH3 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DPH3 |
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 | | | DPH3 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DPH3 |
|  |  |  | | | | Search ThermoFisher Antibodies for DPH3 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DPH3 |
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