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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

DPEP2 Gene

protein-coding   GIFtS: 51
GCID: GC16M068021

dipeptidase 2

  Search for DPEP2
in our new
 Human Malady Compendium 
Biological research products
for DPEP2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Dipeptidase 21 2
EC 3.4.13.193 8
MBD22 5

External Ids:    HGNC: 230281   Entrez Gene: 641742   Ensembl: ENSG000001672617   OMIM: 6099255   UniProtKB: Q9H4A93   

Export aliases for DPEP2 gene to outside databases

Previous GC identifers: GC16M067756 GC16M067797 GC16M066578 GC16M053894


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for DPEP2:
DPEP2 belongs to the membrane-bound dipeptidase (EC 3.4.13.19) family. These enzymes hydrolyze a variety of dipeptides,
including leukotriene D4, the beta-lactam ring of some antibiotics, and cystinyl-bis-glycine (cys-bis-gly) formed
during glutathione degradation (Habib et al., 2003 (PubMed 12738806)).(supplied by OMIM, Mar 2008)

UniProtKB/Swiss-Prot: DPEP2_HUMAN, Q9H4A9
Function: Probable metalloprotease which hydrolyzes leukotriene D4 (LTD4) into leukotriene E4 (LTE4) (By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000016.9  NC_018927.1  NT_010498.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the DPEP2 gene promoter:
         HOXA9   HOXA9B   Nkx2-5   GATA-2   Meis-1b   CBF-A   NF-kappaB2   NF-kappaB1   Meis-1   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidDPEP2 promoter sequence
   Search SABiosciences Chromatin IP Primers for DPEP2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DPEP2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 16q22.1   Ensembl cytogenetic band:  16q22.1   HGNC cytogenetic band: 16q22.1

DPEP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DPEP2 gene location

GeneLoc information about chromosome 16         GeneLoc Exon Structure

GeneLoc location for GC16M068021:  view genomic region     (about GC identifiers)

Start:
68,021,293 bp from pter      End:
68,034,489 bp from pter
Size:
13,197 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: DPEP2_HUMAN, Q9H4A9 (See protein sequence)
Recommended Name: Dipeptidase 2 precursor  
Size: 486 amino acids; 53306 Da
Cofactor: Zinc (By similarity)
Subunit: Homodimer; disulfide-linked (By similarity)
Subcellular location: Membrane; Lipid-anchor, GPI-anchor (By similarity)
Sequence caution: Sequence=AAQ88819.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Secondary accessions: B2RCF8 Q6UX92 Q8TC95
Alternative splicing: 2 isoforms:  Q9H4A9-1   Q9H4A9-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for DPEP2: NX_Q9H4A9

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9H4A9

  • 4/6 DME Specific Peptides for DPEP2 (Q9H4A9) (see all 6)
     DGHNDLP  APVIFSHS  PCQTQDRDA  CASYSELELVTS 

    DPEP2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_071750.1  
    ENSEMBL proteins: 
     ENSP00000377430   ENSP00000459375   ENSP00000458977   ENSP00000462652   ENSP00000463604  
     ENSP00000460169   ENSP00000461250   ENSP00000412549  
    Reactome Protein details: Q9H4A9
    Human Recombinant Protein Products: 
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    Novus Biologicals DPEP2 Lysate
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    Browse ProSpec Recombinant Proteins
    Uscn Proteins for DPEP2

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886plasma membrane TAS--
    GO:0031225anchored to membrane IEA--


    DPEP2 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for DPEP2


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    DPEP2 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR000180 Pept_M19_AS
     IPR008257 Peptidase_M19

    Graphical View of Domain Structure for InterPro Entry Q9H4A9

    ProtoNet protein and cluster: Q9H4A9

    1 Blocks protein family: IPB000180 Renal dipeptidase

    UniProtKB/Swiss-Prot: DPEP2_HUMAN, Q9H4A9
    Similarity: Belongs to the peptidase M19 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: DPEP2_HUMAN, Q9H4A9
    Function: Probable metalloprotease which hydrolyzes leukotriene D4 (LTD4) into leukotriene E4 (LTE4) (By similarity)
    Catalytic activity: Hydrolysis of dipeptides
    Enzyme regulation: Inhibited by L-penicillamine (By similarity)

    Enzyme Number (IUBMB): EC 3.4.13.191 2

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    Gene Ontology (GO): 5 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008235metalloexopeptidase activity IEA--
    GO:0008238exopeptidase activity ----
    GO:0008239dipeptidyl-peptidase activity IEA--
    GO:0016805dipeptidase activity IEA--
    GO:0046872metal ion binding IEA--


    DPEP2 for ontologies           About GeneDecksing


    Animal Models:
         Mouse knock-out Dpep2tm1Lex for DPEP2
         1 MGI phenotypic allele for Dpep2 (no phenotypes)

    DPEP2 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Metabolism
    Metabolism1.00
    Metabolism of lipids and lipoproteins0.34
    2leukotriene biosynthesis
    leukotriene biosynthesis1.00
    Synthesis of Leukotrienes (LT) and Eoxins (EX)0.47
    3Arachidonic acid metabolism
    Arachidonic acid metabolism1.00
    4Busulfan Pathway, Pharmacodynamics
    Busulfan Pathway, Pharmacodynamics1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    1 BioSystems Pathway for DPEP2 
        leukotriene biosynthesis

    4        Reactome Pathways for DPEP2
        Metabolism
    Synthesis of Leukotrienes (LT) and Eoxins (EX)
    Arachidonic acid metabolism
    Metabolism of lipids and lipoproteins

    1 PharmGKB Pathway for DPEP2
        Busulfan Pathway, Pharmacodynamics


    DPEP2 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for DPEP2

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508proteolysis IEA--
    GO:0006692prostanoid metabolic process ----
    GO:0019370leukotriene biosynthetic process ----
    GO:0044281small molecule metabolic process ----


    DPEP2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Compounds for DPEP2 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    Cilastatin sodiumDipeptidase inhibitor[81129-83-1]

    2 HMDB Compounds for DPEP2    About this table
    CompoundSynonyms CAS #PubMed Ids
    10,11-Dihydro-12R-hydroxy-leukotriene E45,12-dihydroxy-6-cysteinyl-7,9,14-eicosatrienoate (see all 9)----
    Leukotriene E4LTE4 (see all 4)75715-89-8--
    Search CenterWatch for drugs/clinical trials and news about DPEP2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for DPEP2 gene: 
    NM_022355.3  

    Unigene Cluster for DPEP2:

    Dipeptidase 2
    Hs.372633  [show with all ESTs]
    Unigene Representative Sequence: NM_022355
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000393847 ENST00000575203(uc002evf.3) ENST00000572888(uc010cey.3)
    ENST00000575510 ENST00000573808 ENST00000268795(uc002evg.3) ENST00000572624
    ENST00000574865 ENST00000574316 ENST00000412757(uc002eve.3)

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    Additional cDNA sequence: 

    AJ295149.1 AK315090.1 AY358454.1 BC024021.1 

    12 DOTS entries:

    DT.312370  DT.100709055  DT.95375439  DT.120665018  DT.95375445  DT.100777825  DT.100022880  DT.92420415 
    DT.95375442  DT.95375443  DT.95375446  DT.95375440 

    24/98 AceView cDNA sequences (see all 98):

    AJ291679 BE670487 BC037243 BE669521 CA308968 AI887572 CA418626 BI909651 
    AY358454 BI836036 AW450595 CA309354 AK057401 CR625664 NM_022357 AI969764 
    CB528462 AW007280 AY358390 AJ295149 BC024021 BI909395 AI768915 BI905812 

    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for DPEP2    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9a · 9b ^ 10
    SP1:                                                        -               
    SP2:                                -                                       


    ECgene alternative splicing isoforms for DPEP2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    DPEP2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GCCCCAGTCC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    DPEP2 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    CD31, CD144 positive cells (Two-step protocol fo...)
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See DPEP2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for DPEP2

    SOURCE GeneReport for Unigene cluster: Hs.372633
        SABiosciences Custom PCR Arrays for DPEP2
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DPEP2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for DPEP2 gene from 4/17 species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves DPEP21 dipeptidase 2 58.22(n)
    59.44(a)
      415714  XM_414081.3  XP_414081.3 
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    57(a)
    1 → many
    LGc(6193338-6207844)
    zebrafish
    (Danio rerio)
    Actinopterygii LOC1005349951 dipeptidase 3-like 58.19(n)
    57.31(a)
      100534995  XM_003198973.1  XP_003199021.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG344201 CG34420 53.35(n)
    47.49(a)
      5740513  NM_001104120.1  NP_001097590.1 


    ENSEMBL Gene Tree for DPEP2 (if available)
    TreeFam Gene Tree for DPEP2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for DPEP2 gene
    DPEP32  DPEP12  
    2 SIMAP similar genes for DPEP2 using alignment to 7 protein entries:     DPEP2_HUMAN (see all proteins):
    DPEP3    DPEP1

    DPEP2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    4 NCBI SNPs in DPEP2 are shown     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 16 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs563034871,2
    C,F,--68029739(+) GTGCGC/TGCCTG 1 -- int15Minor allele frequency- T:0.19WA NA EA 362
    rs711459841,2
    ----68021070(+) ATAAATAAA/-  
            
    AAGAG
    1 -- int11Minor allele frequency- -:0.00NA 2
    rs80599041,2
    ----68029664(+) gagttC/Tgagac 1 -- int10--------
    rs354215121,2
    ----68030902(+) AATGA-/TATTATTA 1 -- int10--------

    HapMap Linkage Disequilibrium report for DPEP2 (68021293 - 68034489 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for DPEP2: --

    SABiosciences Cancer Mutation PCR Assays
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    DNA2.0 Custom Variant and Variant Library Synthesis for DPEP2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    DPEP2 for disorders           About MalaCards

    DPEP2 for disorders           About GeneDecksing

    OMIM gene information: 609925    OMIM disorders: --


    Export disorders for DPEP2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for DPEP2 gene, integrated from 9 sources (see all 13):
    (articles sorted by number of sources associating them with DPEP2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. (PubMed id 16335952)1, 2 Liu T.... Smith R.D. (2005)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    4. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2 Clark H.F.... Gray A.M. (2003)
    5. The Brd4 extraterminal domain confers transcription a ctivation independent of pTEFb by recruiting multiple proteins, including NSD3. (PubMed id 21555454)1 Rahman S....Howley P.M. (2011)
    6. Newly identified loci that influence lipid concentrations and risk of coronary artery disease. (PubMed id 18193043)1 Willer C.J....Abecasis G.R. (2008)
    7. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
    8. The sequence and analysis of duplication-rich human chromosome 16. (PubMed id 15616553)2 Martin J.... Pennacchio L.A. (2004)
    9. Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions. (PubMed id 15342556)1 Suzuki Y.... Sugano S. (2004)
    10. Identification of two additional members of the membr ane-bound dipeptidase family. (PubMed id 12738806)1 Habib G.M....Lieberman M.W. (2003)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 64174 HGNC: 23028 AceView: DPEP2andDPEP3 Ensembl:ENSG00000167261 euGenes: HUgn64174
    ECgene: DPEP2 H-InvDB: DPEP2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for DPEP2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for DPEP2 gene:
    Search GeneIP for patents involving DPEP2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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