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DNAJB3 Gene

pseudogene   GIFtS: 30
GCID: GC02M234651

DnaJ (Hsp40) Homolog, Subfamily B, Member 3

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
DnaJ (Hsp40) Homolog, Subfamily B, Member 31 2
HCG32 3
DnaJ Homolog Subfamily B Member 32

External Ids:    HGNC: 323971   Entrez Gene: 4140612   Ensembl: ENSG000002278027   UniProtKB: Q8WWF63   

Export aliases for DNAJB3 gene to outside databases

Previous GC identifers: GC02U901524 GC02M234318 GC02M226451


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for DNAJB3 Gene:
DNAJB3 (DnaJ (Hsp40) homolog, subfamily B, member 3) is a pseudogene.

UniProtKB/Swiss-Prot: DNJB3_HUMAN, Q8WWF6
Function: May operate as a co-chaperone of the male germ cell- and haploid stage-specific Hsp70 proteins
(Potential)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Regulatory transcription factor binding sites in the DNAJB3 gene promoter:
         AML1a   p53   p300   NRSF form 2   HNF-4alpha1   Arnt   PPAR-alpha   HOXA5   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidDNAJB3 promoter sequence
   Search Chromatin IP Primers for DNAJB3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat DNAJB3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q37   Ensembl cytogenetic band:  2q37.1   HGNC cytogenetic band: 2q37

DNAJB3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DNAJB3 gene location

GeneLoc information about chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M234651:  view genomic region     (about GC identifiers)

Start:
234,651,396 bp from pter      End:
234,652,661 bp from pter
Size:
1,266 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: DNJB3_HUMAN, Q8WWF6 (See protein sequence)
Recommended Name: DnaJ homolog subfamily B member 3  
Size: 145 amino acids; 16559 Da
Caution: Due to a premature stop codon, the human protein is truncated and lacks the DNAJB3 canonical C-terminal
domain. However, expression studies suggest the existence of the mRNA and protein in human
1 PDB 3D structure from and Proteopedia for DNAJB3:
2EJ7 (3D)    

Explore the universe of human proteins at neXtProt for DNAJB3: NX_Q8WWF6

Explore proteomics data for DNAJB3 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys29
  • Modification sites at PhosphoSitePlus

  • See DNAJB3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_001001394.1  

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    DNAJ: Heat shock proteins / DNAJ (HSP40)

    2 InterPro protein domains:
     IPR001623 DnaJ_domain
     IPR018253 DnaJ_domain_CS

    Graphical View of Domain Structure for InterPro Entry Q8WWF6

    ProtoNet protein and cluster: Q8WWF6

    UniProtKB/Swiss-Prot: DNJB3_HUMAN, Q8WWF6
    Similarity: Contains 1 J domain


    Find genes that share domains with DNAJB3           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: DNJB3_HUMAN, Q8WWF6
    Function: May operate as a co-chaperone of the male germ cell- and haploid stage-specific Hsp70 proteins
    (Potential)

    Phenotypes:
         1 MGI phenotypic allele for Dnajb3 (no phenotypes)


    Find genes that share phenotypes with DNAJB3           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Dnajb3tm1(KOMP)Vlcg for DNAJB3

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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for DNAJB3
    Interactions:

        Search GeneGlobe Interaction Network for DNAJB3

    4 Interacting proteins for DNAJB3 (Q8WWF62, 3) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    EWSR1Q018443I2D: score=2 
    --Q9BWA23, 2MINT-66355 I2D: score=1 
    --Q9BWA23, 2MINT-66355 I2D: score=1 
    --Q96MX43I2D: score=1 
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for DNAJB3 (DNJB3)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for DNAJB3 gene: 
    NM_001001394.3  

    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000449667(uc002vuz.3) ENST00000446806
    miRNA
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    hsa-miR-323-5p hsa-miR-578 hsa-miR-876-3p hsa-miR-3190
    SwitchGear 3'UTR luciferase reporter plasmidDNAJB3 3' UTR sequence
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    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    DNAJB3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CATTTATTAT
    DNAJB3 Expression
    About this image


    DNAJB3 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 2) fully expand
     
     Gonad (Reproductive System)
             Primordial Germ Cells Primitive Gonad
     
     Testis (Reproductive System)
    DNAJB3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    DNAJB3 Protein Expression

    UniProtKB/Swiss-Prot: DNJB3_HUMAN, Q8WWF6
    Tissue specificity: Expressed in sperm (at protein level)

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DNAJB3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of human and mouse.

    Orthologs for DNAJB3 gene from 1 species (see representative species )    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Dnajb35 DnaJ (Hsp40) homolog, subfamily B, member 3   --   1 (44.55 cM) 88204732 


    ENSEMBL Gene Tree for DNAJB3 (if available)
    TreeFam Gene Tree for DNAJB3 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for DNAJB3 gene
    18 SIMAP similar genes for DNAJB3 using alignment to 1 protein entry:     DNJB3_HUMAN:
    DNAJB6    DNAJB8    DNAJB7    DNAJB2    DNAJC5B    DNAJC16
    DNAJB4    DNAJB5    DNAJC17    DNAJC21    DNAJB1    DNAJC5G
    DNAJC10    DNAJB12    DNAJC11    DNAJC18    DNAJA4    DNAJC7

    Find genes that share paralogs with DNAJB3           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    2 SNPs for DNAJB3    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 2 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs341504861,2,4
    ----see VAR_0611432 mis40--------
    rs346226151,2,4
    ----see VAR_0611422 mis40--------

    HapMap Linkage Disequilibrium report for DNAJB3 (234651396 - 234652661 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 8 variations for DNAJB3:    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv769e199CNV Deletion23128226
    esv2422005CNV Deletion20811451
    nsv498958CNV Loss21111241
    nsv519815CNV Loss19592680
    nsv3215CNV Loss18451855
    esv25622CNV Loss19812545
    nsv433211CNV Loss18776910
    nsv514125CNV Loss21397061

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for DNAJB3 gene, integrated from 10 sources (see all 13):
    (articles sorted by number of sources associating them with DNAJB3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Bcl2-associated athanogene 3 interactome analysis reveals a new role in modulating proteasome activity. (PubMed id 23824909)1 Chen Y....Ge F. (amp 2013)
    2. Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. (PubMed id 23000965)1 Povlsen L.K....Choudhary C. (Nat. Cell Biol. 2012)
    3. Systematic and quantitative assessment of the ubiquitin-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (Mol. Cell 2011)
    4. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (amp 2011)
    5. Global identification of modular cullin-RING ligase substrates. (PubMed id 21963094)1 Emanuele M.J....Elledge S.J. (Cell 2011)
    6. Structure of msj-1 gene in mice and humans: a possible role in the regulation of male reproduction. (PubMed id 18184612)2 Meccariello R....Fasano S. (Gen. Comp. Endocrinol. 2008)
    7. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (Genomics 2007)
    8. Towards a proteome-scale map of the human protein-protein interaction network. (PubMed id 16189514)1 Rual J.F....Vidal M. (Nature 2005)
    9. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)2 Hillier L.W.... Wilson R.K. (Nature 2005)
    10. Structure of msj-1 gene: a comparative analysis. (PubMed id 15891074)1 Meccariello R....Fasano S. (Ann. N. Y. Acad. Sci. 2005)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 414061 HGNC: 32397 Ensembl:ENSG00000227802 euGenes: HUgn414061 ECgene: DNAJB3
    H-InvDB: DNAJB3

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for DNAJB3 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for DNAJB3 gene:
    Search GeneIP for patents involving DNAJB3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

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