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Aliases for DHFRL1 Gene

Aliases for DHFRL1 Gene

  • Dihydrofolate Reductase-Like 1 2 3
  • Dihydrofolate Reductase-Like Protein 1 3 4
  • Dihydrofolate Reductase Pseudogene 4 2 3
  • EC 4 63
  • DHFRP4 3 4
  • Dihydrofolate Reductase, Mitochondrial 3

External Ids for DHFRL1 Gene

Previous HGNC Symbols for DHFRL1 Gene

  • DHFRP4

Previous GeneCards Identifiers for DHFRL1 Gene

  • GC03M095260
  • GC03M093766
  • GC03M091139

Summaries for DHFRL1 Gene

GeneCards Summary for DHFRL1 Gene

DHFRL1 (Dihydrofolate Reductase-Like 1) is a Protein Coding gene. Diseases associated with DHFRL1 include intrinsic factor deficiency and methylmalonic aciduria and homocystinuria type cblg. Among its related pathways are Disease and Metabolism. GO annotations related to this gene include mRNA binding and dihydrofolate reductase activity. An important paralog of this gene is DHFR.

UniProtKB/Swiss-Prot for DHFRL1 Gene

  • Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.

No data available for Entrez Gene Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DHFRL1 Gene

Genomics for DHFRL1 Gene

Regulatory Elements for DHFRL1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for DHFRL1 Gene

94,047,836 bp from pter
94,063,389 bp from pter
15,554 bases
Minus strand

Genomic View for DHFRL1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for DHFRL1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DHFRL1 Gene

Proteins for DHFRL1 Gene

  • Protein details for DHFRL1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Dihydrofolate reductase, mitochondrial
    Protein Accession:
    Secondary Accessions:
    • D3DN30
    • Q6P4I9

    Protein attributes for DHFRL1 Gene

    187 amino acids
    Molecular mass:
    21620 Da
    Quaternary structure:
    No Data Available

neXtProt entry for DHFRL1 Gene

Proteomics data for DHFRL1 Gene at MOPED

Selected DME Specific Peptides for DHFRL1 Gene

Post-translational modifications for DHFRL1 Gene

  • Ubiquitination at Lys56
  • Modification sites at PhosphoSitePlus

Other Protein References for DHFRL1 Gene

Domains for DHFRL1 Gene

Protein Domains for DHFRL1 Gene

Suggested Antigen Peptide Sequences for DHFRL1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Q86XF0
  • Contains 1 DHFR (dihydrofolate reductase) domain.
  • Belongs to the dihydrofolate reductase family.
genes like me logo Genes that share domains with DHFRL1: view

No data available for Gene Families for DHFRL1 Gene

Function for DHFRL1 Gene

Molecular function for DHFRL1 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=209 uM for dihydrofolate {ECO:0000269 PubMed:21876184}; KM=20 uM for NADPH {ECO:0000269 PubMed:21876184};
UniProtKB/Swiss-Prot CatalyticActivity: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
UniProtKB/Swiss-Prot Function: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.

Enzyme Numbers (IUBMB) for DHFRL1 Gene

Gene Ontology (GO) - Molecular Function for DHFRL1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003729 mRNA binding IDA 21876184
GO:0004146 dihydrofolate reductase activity IMP 21876188
GO:0050661 NADP binding IEA --
genes like me logo Genes that share ontologies with DHFRL1: view

Animal Model Products

CRISPR Products

miRNA for DHFRL1 Gene

miRTarBase miRNAs that target DHFRL1

No data available for Phenotypes , Animal Models , Transcription Factor Targeting and HOMER Transcription for DHFRL1 Gene

Localization for DHFRL1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DHFRL1 Gene

Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.

Subcellular locations from

Jensen Localization Image for DHFRL1 Gene COMPARTMENTS Subcellular localization image for DHFRL1 gene
Compartment Confidence
mitochondrion 5
extracellular 1
nucleus 1

Gene Ontology (GO) - Cellular Components for DHFRL1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005739 mitochondrion IDA 21876184
GO:0005743 mitochondrial inner membrane IMP 21876188
GO:0005759 mitochondrial matrix IMP 21876188
genes like me logo Genes that share ontologies with DHFRL1: view

Pathways for DHFRL1 Gene

SuperPathways for DHFRL1 Gene

Superpath Contained pathways
1 Defective BTD causes biotidinase deficiency
2 One carbon pool by folate
3 Metabolism
4 Disease
5 Folate biosynthesis
genes like me logo Genes that share pathways with DHFRL1: view

UniProtKB/Swiss-Prot Q86XF0-DYRL1_HUMAN

  • Pathway: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1

Interacting Proteins for DHFRL1 Gene

Gene Ontology (GO) - Biological Process for DHFRL1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006545 glycine biosynthetic process IEA --
GO:0006730 one-carbon metabolic process IEA --
GO:0009165 nucleotide biosynthetic process IEA --
GO:0046105 thymidine biosynthetic process IDA 21876188
GO:0046653 tetrahydrofolate metabolic process IDA 21876184
genes like me logo Genes that share ontologies with DHFRL1: view

Transcripts for DHFRL1 Gene

Unigene Clusters for DHFRL1 Gene

Dihydrofolate reductase-like 1:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for DHFRL1

Primer Products

  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for DHFRL1 Gene

No ASD Table

Relevant External Links for DHFRL1 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for DHFRL1 Gene

mRNA expression in normal human tissues for DHFRL1 Gene

SOURCE GeneReport for Unigene cluster for DHFRL1 Gene Hs.718516

mRNA Expression by UniProt/SwissProt for DHFRL1 Gene

Tissue specificity: Expressed in numerous cell lines.
genes like me logo Genes that share expressions with DHFRL1: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Protein expression for DHFRL1 Gene

Orthologs for DHFRL1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for DHFRL1 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia DHFRL1 35
  • 99.28 (n)
  • 97.85 (a)
  • 98 (a)
(Bos Taurus)
Mammalia DHFR 36
  • 83 (a)
(Canis familiaris)
Mammalia -- 36
  • 88 (a)
-- 36
  • 84 (a)
(Mus musculus)
Mammalia Dhfr 36
  • 87 (a)
(Monodelphis domestica)
Mammalia -- 36
  • 78 (a)
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 60 (a)
(Gallus gallus)
Aves DHFR 36
  • 68 (a)
(Anolis carolinensis)
Reptilia -- 36
  • 71 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8788 35
(Danio rerio)
Actinopterygii dhfr 36
  • 57 (a)
fruit fly
(Drosophila melanogaster)
Insecta Dhfr 36
  • 46 (a)
(Caenorhabditis elegans)
Secernentea dhfr-1 36
  • 32 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes DFR1 36
  • 29 (a)
Species with no ortholog for DHFRL1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for DHFRL1 Gene

Gene Tree for DHFRL1 (if available)
Gene Tree for DHFRL1 (if available)

Paralogs for DHFRL1 Gene

Paralogs for DHFRL1 Gene

Selected SIMAP similar genes for DHFRL1 Gene using alignment to 2 proteins: Pseudogenes for DHFRL1 Gene

genes like me logo Genes that share paralogs with DHFRL1: view

Variants for DHFRL1 Gene

Sequence variations from dbSNP and Humsavar for DHFRL1 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type MAF
rs10454213 -- 94,057,892(+) GCATT(A/G)TAAGG downstream-variant-500B
rs11545820 -- 94,059,370(+) CACAA(C/T)GCCCA utr-variant-3-prime
rs11545821 -- 94,059,358(+) ACAGG(C/T)GCGTG utr-variant-3-prime
rs11545822 -- 94,059,495(+) ACTCC(C/T)GGCCA utr-variant-3-prime
rs11545823 -- 94,059,352(+) GAGAC(C/T)ACAGG utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for DHFRL1 Gene

Variant ID Type Subtype PubMed ID
nsv877162 CNV Gain 21882294

Relevant External Links for DHFRL1 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DHFRL1 Gene

Disorders for DHFRL1 Gene

genes like me logo Genes that share disorders with DHFRL1: view

No data available for UniProtKB/Swiss-Prot for DHFRL1 Gene

Publications for DHFRL1 Gene

  1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  2. The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1) is expressed and functional. (PMID: 21876184) McEntee G. … Parle-McDermott A. (Proc. Natl. Acad. Sci. U.S.A. 2011) 3 4
  3. Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria. (PMID: 21876188) Anderson D.D. … Stover P.J. (Proc. Natl. Acad. Sci. U.S.A. 2011) 3 4
  4. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PMID: 12477932) Strausberg R.L. … Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002) 2 3
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3

Products for DHFRL1 Gene

Sources for DHFRL1 Gene

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