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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

DGKK Gene

protein-coding   GIFtS: 50
GCID: GC0XM050128

diacylglycerol kinase, kappa

 Explore 1 disease affiliated with
DGKK via our new
 Human Malady Compendium 
Biological research products
for DGKK
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Diacylglycerol Kinase, Kappa1 2     DAG Kinase Kappa2 3
Diglyceride Kinase Kappa2 3     EC 2.7.1.1073 8
142 KDa Diacylglycerol Kinase2 3     Diacylglycerol Kinase Kappa2
DGK-Kappa1     

External Ids:    HGNC: 323951   Entrez Gene: 1391892   Ensembl: ENSG000002044667   OMIM: 3008375   UniProtKB: Q5KSL63   

Export aliases for DGKK gene to outside databases

Previous GC identifers: GC0XM049944 GC0XM047555


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for DGKK:
The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid.
The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been
associated with hypospadias. (provided by RefSeq, Mar 2011)

UniProtKB/Swiss-Prot: DGKK_HUMAN, Q5KSL6
Function: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA)

summary for DGKK:
Diacylglycerol kinases (DGKs) are a group of ten enzymes (DGKalpha, DGKbeta, DGKgamma, DGKdelta, DGKepsilon,
DGKzeta, DGKeta, DGKtheta, DGKiota and DGKkappa) that metabolize 1,2,diacylglycerol (DAG) to produce
phosphatidic acid (PA). They all contain a conserved C'-terminal catalytic domain, two cysteine-rich
Zn2+-finger motifs that bind DAG and an ATP-binding site, whilst the regulatory domain varies. DGKs are
widely distributed throughout mammalian tissues and different isozymes have different subcellular
localizations; some are cytosolic (for example DGKalpha), some are membrane-bound (for example DGKgamma is
associated with the Golgi membrane) and some are nuclear (for example DGKzeta. DGKs have diverse biological
roles as they participate in signal transduction by modulating levels of DAG in a variety of cellular
responses to extracellular stimuli.




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000023.10  NC_018934.1  NT_011638.13  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the DGKK gene promoter:
         Nkx3-1   Nkx3-1 v4   Nkx3-1 v1   Evi-1   GATA-1   Nkx3-1 v2   Nkx3-1 v3   Cart-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidDGKK promoter sequence
   Search SABiosciences Chromatin IP Primers for DGKK

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DGKK


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.22   Ensembl cytogenetic band:  Xp11.22   HGNC cytogenetic band: Xp11.22

DGKK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DGKK gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XM050128:  view genomic region     (about GC identifiers)

Start:
50,108,408 bp from pter      End:
50,213,737 bp from pter
Size:
105,330 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: DGKK_HUMAN, Q5KSL6 (See protein sequence)
Recommended Name: Diacylglycerol kinase kappa  
Size: 1271 amino acids; 141829 Da
Subunit: Does not form homooligomers
Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein
Secondary accessions: B2RP91

Explore the universe of human proteins at neXtProt for DGKK: NX_Q5KSL6

Post-translational modifications:

  • Phosphorylated at Tyr-78 by some member of the SRC family in response to H(2)O(2)1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q5KSL6

  • 4/5 DME Specific Peptides for DGKK (Q5KSL6) (see all 5)
     QVDGEAW  HHRIAQC  PVQVDGE  PLGTGNDL 

    DGKK Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_001013764.1  Reactome Protein details: Q5KSL6
    Human Recombinant Protein Products: 
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    Browse Proteins at Uscn

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IEA--
    GO:0005886plasma membrane TAS--


    DGKK for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    DGKK for domains           About GeneDecksing

    5 InterPro domains/families:
     IPR001206 Diacylglycerol_kinase_cat_dom
     IPR002219 Prot_Kinase_C-like_PE/DAG-bd
     IPR000756 Diacylglycerol_kin_accessory
     IPR011993 PH_like_dom
     IPR001849 Pleckstrin_homology

    Graphical View of Domain Structure for InterPro Entry Q5KSL6

    ProtoNet protein and cluster: Q5KSL6

    1 Blocks protein family: IPB000756 Diacylglycerol kinase accessory domain (presumed)

    UniProtKB/Swiss-Prot: DGKK_HUMAN, Q5KSL6
    Similarity: Belongs to the eukaryotic diacylglycerol kinase family
    Similarity: Contains 1 DAGKc domain
    Similarity: Contains 1 PH domain
    Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: DGKK_HUMAN, Q5KSL6
    Function: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA)
    Catalytic activity: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
    Enzyme regulation: Inhibited in response to H(2)O(2)

    Enzyme Number (IUBMB): EC 2.7.1.1071 2

    miRNA
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    Inhib. RNA
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    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004143diacylglycerol kinase activity IDA16210324
    GO:0005524ATP binding IEA--
    GO:0005543phospholipid binding IEA--
    GO:0046872metal ion binding IEA--


    DGKK for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Signaling by GPCR
    Signaling by GPCR1.00
    Signal Transduction0.56
    GPCR downstream signaling0.89
    2Platelet activation, signaling and aggregation
    Platelet activation, signaling and aggregation1.00
    Hemostasis0.43
    3G alpha (q) signalling events
    G alpha (q) signalling events1.00
    Gastrin-CREB signalling pathway via PKC and MAPK0.90
    4Effects of PIP2 hydrolysis
    Effects of PIP2 hydrolysis1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    5/8        Reactome Pathways for DGKK (see all 8)
        GPCR downstream signaling
    Hemostasis
    Effects of PIP2 hydrolysis
    Signal Transduction
    Gastrin-CREB signalling pathway via PKC and MAPK



    DGKK for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for DGKK

    Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006979response to oxidative stress IDA16210324
    GO:0007205protein kinase C-activating G-protein coupled receptor signaling pathway IEA--
    GO:0007596blood coagulation TAS--
    GO:0030168platelet activation TAS--
    GO:0035556intracellular signal transduction IDA16210324


    DGKK for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    EMD Millipore small molecules for DGKK:
    Small Molecule - inhibitor
    Browse drugs & compounds from Enzo Life Sciences

    Compounds for DGKK available from Tocris Bioscience    About this table
    CompoundAction CAS #
    R 59-022Diacylglycerol kinase inhibitor; increases PKC activity[93076-89-2]
    DioctanoylglycolDiacylglycerol kinase inhibitor[627-86-1]

    10/855 HMDB Compounds for DGKK (see all 855)    About this table
    CompoundSynonyms CAS #PubMed Ids
    DG(16:0e/18:0/0:0)2-Octadecanoyl-1-hexadecyl-sn-glycerol (see all 2)--12486725
    DG(14:0/14:0/0:0)DAG(14:0/14:0) (see all 9)----
    DG(14:0/14:1(9Z)/0:0)DAG(14:0/14:1) (see all 15)----
    DG(14:0/15:0/0:0)Diacylglycerol(29:0) (see all 9)----
    DG(14:0/16:0/0:0)DAG(30:0) (see all 9)----
    DG(14:0/16:1(9Z)/0:0)DG(30:1) (see all 15)----
    DG(14:0/18:0/0:0)DG(14:0/18:0) (see all 9)----
    DG(14:0/18:1(11Z)/0:0)Diacylglycerol (see all 15)----
    DG(14:0/18:1(9Z)/0:0)1-myristoyl-2-oleoyl-sn-glycerol (see all 15)----
    DG(14:0/18:2(9Z,12Z)/0:0)DAG(14:0/18:2n6) (see all 15)----
    Search CenterWatch for drugs/clinical trials and news about DGKK 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for DGKK gene: 
    NM_001013742.2  

    Unigene Cluster for DGKK:

    Diacylglycerol kinase, kappa
    Hs.631770  [show with all ESTs]
    Unigene Representative Sequence: NM_001013742
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000376025(lincRNA)(uc010njr.2) ENST00000546288(lincRNA)

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    Additional cDNA sequence: 

    AB183864.1 BC137319.1 BC137320.1 

    1 DOTS entry:

    DT.99968229 

    1 AceView cDNA sequence:

    BQ924103 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    DGKK expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AATAAGGAGT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    DGKK expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    PlacentaChorioallantoic PlacentaExtraembryonic AngioblastsEndothelium
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Primitive gut tube-like cells (A scalable, suspensi...)

    See DGKK Protein Expression from SPIRE MOPED and PaxDB
    SOURCE GeneReport for Unigene cluster: Hs.631770

    UniProtKB/Swiss-Prot: DGKK_HUMAN, Q5KSL6
    Tissue specificity: Expressed in testis, and to a lesser extent in placenta

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of human and mouse.

    Orthologs for DGKK gene from 1/6 species (see all 6)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Dgkk1 , 5 diacylglycerol kinase kappa1, 5 82.16(n)1
    77.84(a)1
      X (3.07 cM)5
    3313741  NM_177914.31  NP_808582.31 
     68734845 


    ENSEMBL Gene Tree for DGKK (if available)
    TreeFam Gene Tree for DGKK (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    1 NCBI SNPs in DGKK are shown     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr X posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs114155941,2
    ----50121147(+) ATAAG-/GCCTGG 2 G GP fra10--------

    HapMap Linkage Disequilibrium report for DGKK (50108408 - 50213737 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 6 variations for DGKK
         2 CNVs: 83519 96679
         4 Indels: 96680 83520 83521 96681

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    DGKK for disorders           About GeneDecksing

    OMIM gene information: 300837    OMIM disorders: --

    1 disease for DGKK:    About MalaCards
    hypospadias

    Human Genome Epidemiology (HuGE) Navigator: DGKK (1 document)

    Export disorders for DGKK gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for DGKK gene integrated from 9 sources:
    (articles sorted by number of sources associating them with DGKK)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification and characterization of a novel human type II diacylglycerol kinase, DGK kappa. (PubMed id 16210324)1, 2, 3 Imai S.... Sakane F. (2005)
    2. Common variants in DGKK are strongly associated with risk of hypospadias. (PubMed id 21113153)1 van der Zanden L.F....Knoers N.V. (2011)
    3. Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
    5. Insights into the bile acid transportation system: the human ileal lipid-binding protein-cholyltaurine complex and its comparison with homologous structures. (PubMed id 12486725)6 Kurz M....Kramer W. (2003)
    6. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 139189 HGNC: 32395 AceView: LOC139189 Ensembl:ENSG00000204466 euGenes: HUgn139189
    ECgene: DGKK H-InvDB: DGKK

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for DGKK Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for DGKK gene:
    Search GeneIP for patents involving DGKK

    GeneCards and IP:
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    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat DGKK
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     Regulatory tfbs in DGKK promoter
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     Browse ELISAs, CLIAs, Proteins, and Antibodies at Uscn
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    GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 27 Apr 2013

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