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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

DGKA Gene

protein-coding   GIFtS: 68
GCID: GC12P056324

Diacylglycerol Kinase, Alpha 80kDa

(Previous names: diacylglycerol kinase, alpha (80kD))
(Previous symbols: DAGK, DAGK1)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Diacylglycerol Kinase, Alpha 80kDa1 2     DGK-alpha2 3
DAGK11 2 3 5     DAG Kinase Alpha2 3
DAGK1 2 3     EC 2.7.1.1073 8
Diglyceride Kinase Alpha2 3     Diacylglycerol Kinase, Alpha (80kD)1
80 KDa Diacylglycerol Kinase2 3     Diacylglycerol Kinase Alpha2

External Ids:    HGNC: 28491   Entrez Gene: 16062   Ensembl: ENSG000000653577   OMIM: 1258555   UniProtKB: P237433   

Export aliases for DGKA gene to outside databases

Previous GC identifers: GC12M056229 GC12U900027 GC12P056042 GC12P054611 GC12P053363


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for DGKA Gene:
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator
that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways.
It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to
phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same
protein have been identified. (provided by RefSeq, Jul 2008)

GeneCards Summary for DGKA Gene: 
DGKA (diacylglycerol kinase, alpha 80kDa) is a protein-coding gene. Diseases associated with DGKA include aggressive periodontitis, and lung adenocarcinoma, and among its related super-pathways are Signaling by GPCR and Gastrin-CREB signalling pathway via PKC and MAPK. GO annotations related to this gene include diacylglycerol kinase activity and calcium ion binding. An important paralog of this gene is DGKH.

UniProtKB/Swiss-Prot: DGKA_HUMAN, P23743
Function: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the
resynthesis of phosphatidylinositols and attenuating protein kinase C activity

summary for DGKA Gene:
Diacylglycerol kinases (DGKs) are a group of ten enzymes (DGKalpha, DGKbeta, DGKgamma, DGKdelta, DGKepsilon,
DGKzeta, DGKeta, DGKtheta, DGKiota and DGKkappa) that metabolize 1,2,diacylglycerol (DAG) to produce
phosphatidic acid (PA). They all contain a conserved C'-terminal catalytic domain, two cysteine-rich
Zn2+-finger motifs that bind DAG and an ATP-binding site, whilst the regulatory domain varies. DGKs are
widely distributed throughout mammalian tissues and different isozymes have different subcellular
localizations; some are cytosolic (for example DGKalpha), some are membrane-bound (for example DGKgamma is
associated with the Golgi membrane) and some are nuclear (for example DGKzeta. DGKs have diverse biological
roles as they participate in signal transduction by modulating levels of DAG in a variety of cellular
responses to extracellular stimuli.

Gene Wiki entry for DGKA Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NT_029419.12  NC_018923.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the DGKA gene promoter:
         AREB6   Egr-2   p53   c-Myb   c-Ets-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): DGKA promoter sequence
   Search SABiosciences Chromatin IP Primers for DGKA

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DGKA


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.3   Ensembl cytogenetic band:  12q13.2   HGNC cytogenetic band: 12q13.3

DGKA Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DGKA gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P056324:  view genomic region     (about GC identifiers)

Start:
56,321,103 bp from pter      End:
56,347,811 bp from pter
Size:
26,709 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: DGKA_HUMAN, P23743 (See protein sequence)
Recommended Name: Diacylglycerol kinase alpha  
Size: 735 amino acids; 82630 Da
Subunit: Monomer
1 PDB 3D structure from and Proteopedia for DGKA:
1TUZ (3D)    
Secondary accessions: O75481 O75482 O75483 O95217 Q8IZ56 Q8N5Q2
Alternative splicing: 2 isoforms:  P23743-1   P23743-2   (May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay)

Explore the universe of human proteins at neXtProt for DGKA: NX_P23743

Explore proteomics data for DGKA at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_P23743

  • 4/27 DME Specific Peptides for DGKA (P23743) (see all 27)
     GKQGLSC  VYCNLCE  PEKFNSR  KSGGKQG 

    DGKA Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    DGKA Protein Expression
    REFSEQ proteins (4 alternative transcripts): 
    NP_001336.2  NP_958852.1  NP_958853.1  NP_963848.1  

    ENSEMBL proteins: 
     ENSP00000446515   ENSP00000449178   ENSP00000328405   ENSP00000452353   ENSP00000450977  
     ENSP00000451743   ENSP00000446605   ENSP00000448565   ENSP00000447050   ENSP00000450608  
     ENSP00000450359   ENSP00000385792   ENSP00000450999   ENSP00000451814   ENSP00000402307  
     ENSP00000451266   ENSP00000447460   ENSP00000449923   ENSP00000451330   ENSP00000451518  
     ENSP00000377703  
    Reactome Protein details: P23743
    Human Recombinant Protein Products for DGKA: 
    Browse Purified and Recombinant Proteins at EMD Millipore
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    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Proteins for DGKA
    OriGene Protein Over-expression Lysate for DGKA
    OriGene MassSpec for DGKA 
    OriGene Custom Protein Services for DGKA
    GenScript Custom Purified and Recombinant Proteins Services for DGKA
    Novus Biologicals DGKA Proteins
    Novus Biologicals DGKA Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp. 

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005829cytosol IEA--
    GO:0005886plasma membrane TAS--

    DGKA for ontologies           About GeneDecksing



    DGKA Antibody Products: 
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    Novus Biologicals DGKA Antibodies
    Abcam antibodies for DGKA
    Browse Antibodies at Cloud-Clone Corp. 
    ThermoFisher Antibodies for DGKA
    LSBio Antibodies in human, mouse, rat for DGKA 

    Assay Products for DGKA: 
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    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp. 
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    EFHAND: EF-hand domain containing

    5/6 InterPro protein domains (see all 6):
     IPR001206 Diacylglycerol_kinase_cat_dom
     IPR011992 EF-hand-like_dom
     IPR002048 EF_hand_dom
     IPR000756 Diacylglycerol_kin_accessory
     IPR018247 EF_Hand_1_Ca_BS

    Graphical View of Domain Structure for InterPro Entry P23743

    ProtoNet protein and cluster: P23743

    1 Blocks protein domain: IPB000756 Diacylglycerol kinase accessory domain (presumed)

    UniProtKB/Swiss-Prot: DGKA_HUMAN, P23743
    Similarity: Belongs to the eukaryotic diacylglycerol kinase family
    Similarity: Contains 1 DAGKc domain
    Similarity: Contains 2 EF-hand domains
    Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers


    DGKA for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: DGKA_HUMAN, P23743
    Function: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the
    resynthesis of phosphatidylinositols and attenuating protein kinase C activity
    Catalytic activity: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
    Enzyme regulation: Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C

         Genatlas biochemistry entry for DGKA:
    diacylglycerol kinase,86kDa,putative modulator of protein kinase C,in signal transduction

         Enzyme Number (IUBMB): EC 2.7.1.1071 2

         Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003951NAD+ kinase activity IEA--
    GO:0004143diacylglycerol kinase activity IEA--
    GO:0005509calcium ion binding IEA--
    GO:0005524ATP binding IEA--
    GO:0005543phospholipid binding IEA--
         
    DGKA for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for DGKA:
     Increased apoptosis  Increased cell number in G1, s 

         4 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Dgka):
     cellular  hematopoietic system  immune system  reproductive system 

    DGKA for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Dgkatm1Xpz for DGKA

       inGenious Targeting Laboratory - Custom generated mouse model solutions for DGKA 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for DGKA

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for DGKA 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for DGKA 

    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat DGKA
    6 QIAGEN miScript miRNA Assays for microRNAs that regulate DGKA:
    hsa-miR-200c hsa-miR-885-3p hsa-miR-429 hsa-miR-1252 hsa-miR-1827 hsa-miR-200b
    SwitchGear 3'UTR luciferase reporter plasmidDGKA 3' UTR sequence
    Inhib. RNA
    Products:
        
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    Gene Editing
    Products:
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    Sirion Biotech Customized adenovirus for overexpression of DGKA

    Clone
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    GenScript: all cDNA clones in your preferred vector (see all 4): DGKA (NM_001345)
    Sino Biological Human cDNA Clone for DGKA
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DGKA
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DGKA
    Sirion Biotech Customized lentivirus for stable overexpression of DGKA 
                         Customized lentivirus expression plasmids for stable overexpression of DGKA 

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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DGKA


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for DGKA About   (see all 9)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Signaling by GPCR
    Signaling by GPCR0.90
    Signal Transduction0.55
    GPCR downstream signaling0.90
    2G alpha (q) signalling events
    Gastrin-CREB signalling pathway via PKC and MAPK0.90
    G alpha (q) signalling events0.90
    3Platelet activation, signaling and aggregation
    Hemostasis0.43
    Platelet activation, signaling and aggregation0.43
    4Glycerophospholipid biosynthesis
    Glycerophospholipid metabolism0.62
    5Inositol phosphate metabolism
    Phosphatidylinositol signaling system0.61

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 BioSystems Pathway for DGKA
        Calcineurin-regulated NFAT-dependent transcription in lymphocytes


    5/8        Reactome Pathways for DGKA (see all 8)
        GPCR downstream signaling
    Hemostasis
    Effects of PIP2 hydrolysis
    Signal Transduction
    Gastrin-CREB signalling pathway via PKC and MAPK


    4         Kegg Pathways  (Kegg details for DGKA):
        Glycerolipid metabolism
    Glycerophospholipid metabolism
    Metabolic pathways
    Phosphatidylinositol signaling system


    DGKA for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for DGKA

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/45 Interacting proteins for DGKA (P237432, 3 ENSP000003284054) via UniProtKB, MINT, STRING, and/or I2D (see all 45)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SRCP129312, 3, ENSP000003509414MINT-8033370 MINT-8033355 I2D: score=3 STRING: ENSP00000350941
    IL2P605683, ENSP000002267304I2D: score=1 STRING: ENSP00000226730
    IL21Q9HBE43, ENSP000002644974I2D: score=1 STRING: ENSP00000264497
    SMAD2Q157963, ENSP000002621604I2D: score=1 STRING: ENSP00000262160
    SREK1Q8WXA93, ENSP000003345384I2D: score=1 STRING: ENSP00000334538
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007205protein kinase C-activating G-protein coupled receptor signaling pathway IEA--
    GO:0007596blood coagulation TAS--
    GO:0030168platelet activation TAS--
    GO:0035556intracellular signal transduction IEA--

    DGKA for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    DGKA for compounds           About GeneDecksing

    EMD Millipore small molecules for DGKA:
    Small Molecule - inhibitor
    Browse drugs & compounds from Enzo Life Sciences

    Compounds for DGKA available from Tocris Bioscience    About this table
    CompoundAction CAS #
    DioctanoylglycolDiacylglycerol kinase inhibitor[627-86-1]

    10/987 HMDB Compounds for DGKA (see all 987)    About this table
    CompoundSynonyms CAS #PubMed Ids
    DG(16:0e/18:0/0:0)2-Octadecanoyl-1-hexadecyl-sn-glycerol (see all 2)--12486725
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    CalciumCa (see all 2)7440-70-2--
    DG(14:0/14:0/0:0)DAG(14:0/14:0) (see all 9)----
    DG(14:0/14:1(9Z)/0:0)DAG(14:0/14:1) (see all 15)----
    DG(14:0/15:0/0:0)Diacylglycerol(29:0) (see all 9)----
    DG(14:0/16:0/0:0)DAG(30:0) (see all 9)----
    DG(14:0/16:1(9Z)/0:0)DG(30:1) (see all 15)----
    DG(14:0/18:0/0:0)DG(14:0/18:0) (see all 9)----

    1 DrugBank Compound for DGKA    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Vitamin Ealpha-Tocopherol (see all 2)59-02-9target--17139284 17016423

    4 Novoseek inferred chemical compound relationships for DGKA gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 44.8 7 18424699 (3), 17700527 (2), 19491056 (1), 15773894 (1)
    phosphoinositide 29.6 1 12832407 (1)
    lipid 17.3 6 11467959 (3), 12832407 (1)
    calcium 0 1 12832407 (1)

    Search CenterWatch for drugs/clinical trials and news about DGKA

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for DGKA gene (4 alternative transcripts): 
    NM_001345.4  NM_201444.2  NM_201445.1  NM_201554.1  

    Unigene Cluster for DGKA:

    Diacylglycerol kinase, alpha 80kDa
    Hs.524488  [show with all ESTs]
    Unigene Representative Sequence: AK226134
    18/49 Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 49):
    ENST00000546878 ENST00000546995 ENST00000547015 ENST00000331886(uc001sih.1 uc001sii.1 uc009zod.1 uc001sij.3)
    ENST00000555090 ENST00000549629 ENST00000555218 ENST00000548479 ENST00000547324
    ENST00000553084 ENST00000547358(uc009zoc.1) ENST00000548549 ENST00000549368
    ENST00000548407 ENST00000555025 ENST00000551615 ENST00000547535 ENST00000549323

    miRNA
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    6 QIAGEN miScript miRNA Assays for microRNAs that regulate DGKA:
    hsa-miR-200c hsa-miR-885-3p hsa-miR-429 hsa-miR-1252 hsa-miR-1827 hsa-miR-200b
    SwitchGear 3'UTR luciferase reporter plasmidDGKA 3' UTR sequence
    Inhib. RNA
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DGKA
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    Additional mRNA sequence: 

    AF064767.1 AF064768.1 AF064769.1 AF064770.1 AF064771.1 AK226134.1 AK303320.1 AK307685.1 
    AK309939.1 AK310600.1 AY930112.1 BC008292.1 BC023523.2 BC031870.2 X62535.1 

    24/35 DOTS entries (see all 35):

    DT.100649148  DT.95151781  DT.97844658  DT.313862  DT.100649146  DT.100867421  DT.121207978  DT.86859208 
    DT.95136199  DT.95141713  DT.91746710  DT.92436769  DT.95141742  DT.121207996  DT.92028038  DT.92436768 
    DT.121207930  DT.121207932  DT.95141714  DT.97780024  DT.70104591  DT.75103892  DT.75189519  DT.100649147 

    24/217 AceView cDNA sequences (see all 217):

    AW849539 BM910598 CA430340 AK127093 BE397100 AW134838 BQ061404 BQ938230 
    NM_201445 AK122973 BM824780 NM_201554 AL551428 BE744599 CR625683 BE206354 
    AU141305 CR616473 NM_201444 BG752623 BC023523 BQ641370 BQ943057 AI028222 

    GeneLoc Exon Structure

    5/18 Alternative Splicing Database (ASD) splice patterns (SP) for DGKA (see all 18)    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b · 10c ^ 11 ^ 12a · 12b ^ 13 ^ 14a · 14b · 14c ^ 15a ·
    SP1:                                            -           -                 -                 -           -     -     -                             -     -   
    SP2:                                                        -                 -                 -           -     -     -                             -     -   
    SP3:                                            -           -                 -                 -           -     -     -                             -     -   
    SP4:                                                                          -                 -           -     -     -                             -     -   
    SP5:                                                                                                                                                            

    ExUns: 15b ^ 16a · 16b · 16c ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^ 24 ^ 25 ^ 26 ^ 27 ^ 28 ^ 29 ^ 30a · 30b
    SP1:        -           -                 -                             -                                                   
    SP2:        -           -                 -                             -                                                   
    SP3:  -     -           -                 -                             -                                                   
    SP4:        -           -                 -                             -                                                   
    SP5:                    -                 -                                                                                 


    ECgene alternative splicing isoforms for DGKA

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    DGKA expression in normal human tissues (normalized intensities)      DGKA embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CGCACACACA
    DGKA Expression
    About this image


    DGKA expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/12 selected tissues (see all 12) fully expand
     
     Nose (Sensory Organs)    fully expand to see all 2 entries
             sensory organ/nose/nasal cavity   
     
     Thymus (Hematopoietic System)
             Double Negative 2 Thymocytes Thymus
     
     Gut Tube (Gastrointestinal Tract)
             Foregut
     
     Gonad
             XY Germ Cells Testis Cord
     
     Fibroblast
             Human Dermal Fibroblasts-neonate (HDF-n)   

    See DGKA Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for DGKA

    SOURCE GeneReport for Unigene cluster: Hs.524488

    UniProtKB/Swiss-Prot: DGKA_HUMAN, P23743
    Tissue specificity: Lymphocytes and oligodendroglial cells

        SABiosciences Expression via Pathway-Focused PCR Array including DGKA: 
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for DGKA gene from 5/12 species (see all 12)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Dgka1 , 5 diacylglycerol kinase, alpha1, 5 83.45(n)1
    82.99(a)1
      10 (77.14 cM)5
    131391  NM_016811.21  NP_058091.21 
     1287201415 
    lizard
    (Anolis carolinensis)
    Reptilia DGKA6
    Uncharacterized protein
    64(a)
    1 ↔ 1
    GL343198.1(3218030-3334020)
    zebrafish
    (Danio rerio)
    Actinopterygii dgkaa1 diacylglycerol kinase, alpha a 64.56(n)
    63.57(a)
      724010  NM_001045315.1  NP_001038780.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Dgk3 phosphatidylinositol biosynthesis
    diacylglycerol more
    51(a)   43E18   --
    worm
    (Caenorhabditis elegans)
    Secernentea dgk-33 Diacylglycerol kinase 44(a)   III(9234802-9239752)   --


    ENSEMBL Gene Tree for DGKA (if available)
    TreeFam Gene Tree for DGKA (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for DGKA gene
    DGKH2  DGKG2  DGKQ2  DGKB2  DGKD2  DGKI2  DGKE2  DGKZ2  
    6 SIMAP similar genes for DGKA using alignment to 13 protein entries:     DGKA_HUMAN (see all proteins):
    DAGK1    DGKB    DGKG    DGKZ    DGKI    DGKE

    DGKA for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/454 SNPs in DGKA are shown (see all 454)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1181646811,2
    F--56323795(+) AAGCTG/AAGTGC 1 -- us2k11Minor allele frequency- A:0.01NA 120
    rs1467466851,2
    --56323890(+) TCTTAC/TATTGT 4 -- us2k10--------
    rs1392069101,2
    C--56323912(+) AAGTAG/TCTCAG 4 -- us2k10--------
    rs1499667581,2
    --56323939(+) ACACAG/TTGCAC 4 -- us2k10--------
    rs573179281,2
    C--56324151(+) TTTTT-/GTTTTT 4 -- us2k10--------
    rs602618181,2
    C--56324154(+) TTTTT-/GTTTTT 4 -- us2k10--------
    rs597997031,2
    C--56324157(+) TTTTT-/GTTTTT 4 -- us2k10--------
    rs1809624391,2
    --56324273(+) AGCCTC/TGCGAG 4 -- us2k10--------
    rs111717021,2
    C--56324336(+) tagtaG/Aagaca 4 -- us2k11Minor allele frequency- A:0.50NA 2
    rs116153781,2
    H--56324368(+) gatggG/Tctcaa 4 -- us2k10--------

    HapMap Linkage Disequilibrium report for DGKA (56321103 - 56347811 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for DGKA:    About this table     
    Variant IDTypeSubtypePubMed ID
    nsv832424CNV Loss17160897

    SABiosciences Cancer Mutation PCR Assays
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    DNA2.0 Custom Variant and Variant Library Synthesis for DGKA

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 125855    OMIM disorders: --

    8 diseases for DGKA:    About MalaCards
    aggressive periodontitis    lung adenocarcinoma    periodontitis    adenocarcinoma
    endotheliitis    melanoma    gastric cancer    neuronitis


    DGKA for disorders           About GeneDecksing


    Export disorders for DGKA gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for DGKA gene, integrated from 9 sources (see all 64):
    (articles sorted by number of sources associating them with DGKA)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Assignment of the gene for diacylglycerol kinase (DAGK) to human chromosome 12. (PubMed id 8180475)1, 2, 3 Hart T.C.... van Dyke T.E. (1994)
    2. Assignment of the human diacylglycerol kinase gene (DAGK) to 12q13.3 using fluorescence in situ hybridization analysis. (PubMed id 7959783)1, 2, 3 Hart T.C....Pettenati M.J. (1994)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Purification, cDNA-cloning and expression of human diacylglycerol kinase. (PubMed id 2175712)1, 2 Schaap D....van der Bendl R.L. (1990)
    5. Lck-dependent tyrosine phosphorylation of diacylglycerol kinase alpha regulates its membrane association in T cells. (PubMed id 18424699)1, 9 Merino E....Merida I. (2008)
    6. Diacylglycerol kinase alpha regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes. (PubMed id 15870081)1, 9 Alonso R....Izquierdo M. (2005)
    7. Diacylglycerol kinase-alpha phosphorylation by Src on Y335 is required for activation, membrane recruitment and Hgf-induced cell motility. (PubMed id 17700527)1, 9 Baldanzi G....Graziani A. (2008)
    8. Diacylglycerol kinase alpha enhances protein kinase C zeta-dependent phosphorylation at Ser311 of p65/RelA subunit of nuclear factor- kappaB. (PubMed id 19751727)1, 9 Kai M....Sakane F. (2009)
    9. Activation of diacylglycerol kinase alpha is required for VEGF-induced angiogenic signaling in vitro. (PubMed id 15122338)1, 9 Baldanzi G....Graziani A. (2004)
    10. Src-mediated activation of alpha-diacylglycerol kinase is required for hepatocyte growth factor-induced cell motility. (PubMed id 10970854)1, 9 Cutrupi S....Graziani A. (2000)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 1606 HGNC: 2849 AceView: DGKA Ensembl:ENSG00000065357 euGenes: HUgn1606
    ECgene: DGKA Kegg: 1606 H-InvDB: DGKA

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for DGKA Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for DGKA Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for DGKA gene:
    Search GeneIP for patents involving DGKA

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

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