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DGKA Gene

protein-coding   GIFtS: 69
GCID: GC12P056324

Diacylglycerol Kinase, Alpha 80kDa

(Previous names: diacylglycerol kinase, alpha (80kD))
(Previous symbols: DAGK, DAGK1)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Diacylglycerol Kinase, Alpha 80kDa1 2     DGK-alpha2 3
DAGK11 2 3 5     DAG Kinase Alpha2 3
DAGK1 2 3     EC 2.7.1.1073 8
Diglyceride Kinase Alpha2 3     Diacylglycerol Kinase, Alpha (80kD)1
80 KDa Diacylglycerol Kinase2 3     Diacylglycerol Kinase Alpha2

External Ids:    HGNC: 28491   Entrez Gene: 16062   Ensembl: ENSG000000653577   OMIM: 1258555   UniProtKB: P237433   

Export aliases for DGKA gene to outside databases

Previous GC identifers: GC12M056229 GC12U900027 GC12P056042 GC12P054611 GC12P053363


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for DGKA Gene:
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator
that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways.
It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to
phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same
protein have been identified. (provided by RefSeq, Jul 2008)

GeneCards Summary for DGKA Gene:
DGKA (diacylglycerol kinase, alpha 80kDa) is a protein-coding gene. GO annotations related to this gene include NAD+ kinase activity and calcium ion binding. An important paralog of this gene is DGKH.

UniProtKB/Swiss-Prot: DGKA_HUMAN, P23743
Function: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the
resynthesis of phosphatidylinositols and attenuating protein kinase C activity

summary for DGKA Gene:
Diacylglycerol kinases (DGKs) are a group of ten enzymes (DGKalpha, DGKbeta, DGKgamma, DGKdelta, DGKepsilon,
DGKzeta, DGKeta, DGKtheta, DGKiota and DGKkappa) that metabolize 1,2,diacylglycerol (DAG) to produce
phosphatidic acid (PA). They all contain a conserved C'-terminal catalytic domain, two cysteine-rich
Zn2+-finger motifs that bind DAG and an ATP-binding site, whilst the regulatory domain varies. DGKs are
widely distributed throughout mammalian tissues and different isozymes have different subcellular
localizations; some are cytosolic (for example DGKalpha), some are membrane-bound (for example DGKgamma is
associated with the Golgi membrane) and some are nuclear (for example DGKzeta. DGKs have diverse biological
roles as they participate in signal transduction by modulating levels of DAG in a variety of cellular
responses to extracellular stimuli.

Gene Wiki entry for DGKA Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000012.11  NT_029419.13  NC_018923.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the DGKA gene promoter:
         AREB6   Egr-2   p53   c-Myb   c-Ets-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): DGKA promoter sequence
   Search Chromatin IP Primers for DGKA

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat DGKA


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.3   Ensembl cytogenetic band:  12q13.2   HGNC cytogenetic band: 12q13.3

DGKA Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DGKA gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P056324:  view genomic region     (about GC identifiers)

Start:
56,321,103 bp from pter      End:
56,347,811 bp from pter
Size:
26,709 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: DGKA_HUMAN, P23743 (See protein sequence)
Recommended Name: Diacylglycerol kinase alpha  
Size: 735 amino acids; 82630 Da
Subunit: Monomer
1 PDB 3D structure from and Proteopedia for DGKA:
1TUZ (3D)    
Secondary accessions: O75481 O75482 O75483 O95217 Q3ZE25 Q8IZ56 Q8N5Q2
Alternative splicing: 3 isoforms:  P23743-1   P23743-2   P23743-3   (May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay)

Explore the universe of human proteins at neXtProt for DGKA: NX_P23743

Explore proteomics data for DGKA at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys18, Lys239, Lys249, Lys263, Lys353, Lys411, Lys484, Lys493, Lys634
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for DGKA (P23743) (see all 27)
     GKQGLSC  VYCNLCE  PEKFNSR  KSGGKQG 


    See DGKA Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001336.2  NP_958852.1  NP_958853.1  NP_963848.1  

    ENSEMBL proteins: 
     ENSP00000446515   ENSP00000449178   ENSP00000328405   ENSP00000452353   ENSP00000450977  
     ENSP00000451743   ENSP00000446605   ENSP00000448565   ENSP00000447050   ENSP00000450608  
     ENSP00000450359   ENSP00000385792   ENSP00000450999   ENSP00000451814   ENSP00000402307  
     ENSP00000451266   ENSP00000447460   ENSP00000449923   ENSP00000451330   ENSP00000451518  
     ENSP00000377703  
    Reactome Protein details: P23743

    DGKA Human Recombinant Protein Products:

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    Novus Biologicals DGKA Proteins
    Novus Biologicals DGKA Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
    Search eBioscience for Proteins for DGKA 

     
    antibodies-online proteins for DGKA (9 products) 

     
    antibodies-online peptides for DGKA

    DGKA Antibody Products:

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    antibodies-online antibodies for DGKA (63 products) 

    DGKA Assay Products:

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    Browse ELISAs at Cloud-Clone Corp.
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    antibodies-online kits for DGKA (10 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    EFHAND: EF-hand domain containing

    Selected InterPro protein domains (see all 7):
     IPR001206 Diacylglycerol_kinase_cat_dom
     IPR011992 EF-hand-dom_pair
     IPR002048 EF_hand_dom
     IPR000756 Diacylglycerol_kin_accessory
     IPR018247 EF_Hand_1_Ca_BS

    Graphical View of Domain Structure for InterPro Entry P23743

    ProtoNet protein and cluster: P23743

    1 Blocks protein domain: IPB000756 Diacylglycerol kinase accessory domain (presumed)

    UniProtKB/Swiss-Prot: DGKA_HUMAN, P23743
    Similarity: Belongs to the eukaryotic diacylglycerol kinase family
    Similarity: Contains 1 DAGKc domain
    Similarity: Contains 2 EF-hand domains
    Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers


    Find genes that share domains with DGKA           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: DGKA_HUMAN, P23743
    Function: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the
    resynthesis of phosphatidylinositols and attenuating protein kinase C activity
    Catalytic activity: ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
    Enzyme regulation: Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C

         Genatlas biochemistry entry for DGKA:
    diacylglycerol kinase,86kDa,putative modulator of protein kinase C,in signal transduction

         Enzyme Number (IUBMB): EC 2.7.1.1071 2

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003951NAD+ kinase activity IEA--
    GO:0004143diacylglycerol kinase activity IEA--
    GO:0005509calcium ion binding IEA--
    GO:0005524ATP binding IEA--
    GO:0005543phospholipid binding IEA--
         
    Find genes that share ontologies with DGKA           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for DGKA:
     Increased apoptosis  Increased cell number in G1, s 

         3 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Dgka):
     hematopoietic system  immune system  reproductive system 

    Find genes that share phenotypes with DGKA           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Dgkatm1Xpz for DGKA

       genOway: Develop your customized and physiologically relevant rodent model for DGKA

    miRNA
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    miRTarBase miRNAs that target DGKA:
    hsa-mir-124-3p (MIRT023096), hsa-mir-484 (MIRT042155)

    Block miRNA regulation of human, mouse, rat DGKA using miScript Target Protectors
    6 qRT-PCR Assays for microRNAs that regulate DGKA:
    hsa-miR-200c hsa-miR-885-3p hsa-miR-429 hsa-miR-1252 hsa-miR-1827 hsa-miR-200b
    SwitchGear 3'UTR luciferase reporter plasmidDGKA 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for DGKA
    Predesigned siRNA for gene silencing in human, mouse, rat DGKA

    Gene Editing
    Products:
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    OriGene ORF clones in mouse, rat for DGKA
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    GenScript: all cDNA clones in your preferred vector (see all 4): DGKA (NM_001345)
    Sino Biological Human cDNA Clone for DGKA
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DGKA
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DGKA
    Addgene plasmids for DGKA 

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for DGKA 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DGKA


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    plasma membrane4
    cytosol3
    nucleus1

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005829cytosol IEA--
    GO:0005886plasma membrane TAS--

    Find genes that share ontologies with DGKA           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for DGKA About   (see all 9)  
    See pathways by source

    SuperPathContained pathways About
    1Signaling by GPCR
    Signaling by GPCR0.90
    Signal Transduction0.58
    GPCR downstream signaling0.90
    2Gastrin-CREB signalling pathway via PKC and MAPK
    Gastrin-CREB signalling pathway via PKC and MAPK0.90
    G alpha (q) signalling events0.90
    3Hemostasis
    Hemostasis0.43
    Platelet activation, signaling and aggregation0.43
    4Glycerophospholipid biosynthesis
    Glycerophospholipid metabolism0.62
    5Inositol phosphate metabolism (KEGG)
    Phosphatidylinositol signaling system0.61


    Find genes that share SuperPaths with DGKA           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 BioSystems Pathway for DGKA
        Calcineurin-regulated NFAT-dependent transcription in lymphocytes


    1 Reactome Pathway for DGKA
        Effects of PIP2 hydrolysis


    4 Kegg Pathways  (Kegg details for DGKA):
        Glycerolipid metabolism
    Glycerophospholipid metabolism
    Metabolic pathways
    Phosphatidylinositol signaling system

        Pathway & Disease-focused RT2 Profiler PCR Array including DGKA: 
              T-Cell Anergy & Immune Tolerance in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for DGKA

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for DGKA (P237432, 3 ENSP000003284054) via UniProtKB, MINT, STRING, and/or I2D (see all 49)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SRCP129312, 3, ENSP000003509414MINT-8033370 MINT-8033355 I2D: score=3 STRING: ENSP00000350941
    IL2P605683, ENSP000002267304I2D: score=1 STRING: ENSP00000226730
    IL21Q9HBE43, ENSP000002644974I2D: score=1 STRING: ENSP00000264497
    SMAD2Q157963, ENSP000002621604I2D: score=1 STRING: ENSP00000262160
    SREK1Q8WXA93, ENSP000003345384I2D: score=1 STRING: ENSP00000334538
    About this table

    Gene Ontology (GO): 4 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007205protein kinase C-activating G-protein coupled receptor signaling pathway IEA--
    GO:0007596blood coagulation TAS--
    GO:0030168platelet activation TAS--
    GO:0035556intracellular signal transduction IEA--

    Find genes that share ontologies with DGKA           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Compounds for DGKA available from Tocris Bioscience    About this table
    CompoundAction CAS #
    DioctanoylglycolDiacylglycerol kinase inhibitor[627-86-1]

    Selected HMDB Compounds for DGKA (see all 987)    About this table
    CompoundSynonyms CAS #PubMed Ids
    DG(16:0e/18:0/0:0)2-Octadecanoyl-1-hexadecyl-sn-glycerol (see all 2)--12486725
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    CalciumCa (see all 2)7440-70-2--
    DG(14:0/14:0/0:0)DAG(14:0/14:0) (see all 9)----
    DG(14:0/14:1(9Z)/0:0)DAG(14:0/14:1) (see all 15)----
    DG(14:0/15:0/0:0)Diacylglycerol(29:0) (see all 9)----
    DG(14:0/16:0/0:0)DAG(30:0) (see all 9)----
    DG(14:0/16:1(9Z)/0:0)DG(30:1) (see all 15)----
    DG(14:0/18:0/0:0)DG(14:0/18:0) (see all 9)----

    1 DrugBank Compound for DGKA    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Vitamin Ealpha-Tocopherol (see all 2)59-02-9target--17139284 17016423

    4 Novoseek inferred chemical compound relationships for DGKA gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 44.8 7 18424699 (3), 17700527 (2), 19491056 (1), 15773894 (1)
    phosphoinositide 29.6 1 12832407 (1)
    lipid 17.3 6 11467959 (3), 12832407 (1)
    calcium 0 1 12832407 (1)



    Find genes that share compounds with DGKA           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for DGKA gene (4 alternative transcripts): 
    NM_001345.4  NM_201444.2  NM_201445.1  NM_201554.1  

    Unigene Cluster for DGKA:

    Diacylglycerol kinase, alpha 80kDa
    Hs.524488  [show with all ESTs]
    Unigene Representative Sequence: AK226134
    Selected Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 49):
    ENST00000546878 ENST00000546995 ENST00000547015 ENST00000331886(uc001sih.1 uc001sii.1 uc009zod.1 uc001sij.3)
    ENST00000555090 ENST00000549629 ENST00000555218 ENST00000548479 ENST00000547324
    ENST00000553084 ENST00000547358(uc009zoc.1) ENST00000548549 ENST00000549368
    ENST00000548407 ENST00000555025 ENST00000551615 ENST00000547535 ENST00000549323

    miRNA
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    6 qRT-PCR Assays for microRNAs that regulate DGKA:
    hsa-miR-200c hsa-miR-885-3p hsa-miR-429 hsa-miR-1252 hsa-miR-1827 hsa-miR-200b
    SwitchGear 3'UTR luciferase reporter plasmidDGKA 3' UTR sequence
    Inhib. RNA
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    Addgene plasmids for DGKA 
    Primer
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      QuantiFast Probe-based Assays in human, mouse, rat DGKA

    Additional mRNA sequence: 

    AF064767.1 AF064768.1 AF064769.1 AF064770.1 AF064771.1 AK226134.1 AK303320.1 AK307685.1 
    AK309939.1 AK310600.1 AY930112.1 BC008292.1 BC023523.2 BC031870.2 X62535.1 

    Selected DOTS entries (see all 35):

    DT.100649148  DT.95151781  DT.97844658  DT.313862  DT.100649146  DT.100867421  DT.121207978  DT.86859208 
    DT.95136199  DT.95141713  DT.91746710  DT.92436769  DT.95141742  DT.121207996  DT.92028038  DT.92436768 
    DT.121207930  DT.121207932  DT.95141714  DT.97780024  DT.70104591  DT.75103892  DT.75189519  DT.100649147 

    Selected AceView cDNA sequences (see all 217):

    BE744599 AW849539 BM824780 BE397100 CB123870 BQ642739 NM_201445 AI028222 
    BX113252 BQ054457 BG742905 BQ641370 CR616473 BQ024673 AI986394 AK122973 
    BQ428244 AK127093 CR625683 BQ943057 BC031870 BQ057634 BM910598 BG752623 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for DGKA (see all 18)    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b · 10c ^ 11 ^ 12a · 12b ^ 13 ^ 14a · 14b · 14c ^ 15a ·
    SP1:                                            -           -                 -                 -           -     -     -                             -     -   
    SP2:                                                        -                 -                 -           -     -     -                             -     -   
    SP3:                                            -           -                 -                 -           -     -     -                             -     -   
    SP4:                                                                          -                 -           -     -     -                             -     -   
    SP5:                                                                                                                                                            

    ExUns: 15b ^ 16a · 16b · 16c ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^ 24 ^ 25 ^ 26 ^ 27 ^ 28 ^ 29 ^ 30a · 30b
    SP1:        -           -                 -                             -                                                   
    SP2:        -           -                 -                             -                                                   
    SP3:  -     -           -                 -                             -                                                   
    SP4:        -           -                 -                             -                                                   
    SP5:                    -                 -                                                                                 


    ECgene alternative splicing isoforms for DGKA

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    DGKA expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CGCACACACA
    DGKA Expression
    About this image


    DGKA expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 9) fully expand
     
     Blood (Cardiovascular System)    fully expand to see all 3 entries
             T-Cytotoxic Cells Peripheral Blood
     
     Thymus (Hematopoietic System)
             Double Negative 2 Thymocytes Thymus
     
     Gut Tube (Gastrointestinal Tract)
             Foregut
     
     Gonad
             XY Germ Cells Testis Cord
     
     Testis (Reproductive System)
             XY Germ Cells Testis Cord
    DGKA Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    DGKA Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.524488

    UniProtKB/Swiss-Prot: DGKA_HUMAN, P23743
    Tissue specificity: Lymphocytes and oligodendroglial cells

        Pathway & Disease-focused RT2 Profiler PCR Array including DGKA: 
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for DGKA gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Dgka1 , 5 diacylglycerol kinase, alpha1, 5 83.45(n)1
    82.99(a)1
      10 (77.14 cM)5
    131391  NM_016811.21  NP_058091.21 
     1287201415 
    lizard
    (Anolis carolinensis)
    Reptilia DGKA6
    diacylglycerol kinase, alpha 80kDa
    64(a)
    1 ↔ 1
    GL343198.1(3218030-3334020)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia dgka1 diacylglycerol kinase, alpha 80kDa 64.79(n)
    66.44(a)
      100124931  XM_002934531.2  XP_002934577.1 
    zebrafish
    (Danio rerio)
    Actinopterygii dgkaa1 diacylglycerol kinase, alpha a 64.6(n)
    63.57(a)
      724010  NM_001045315.1  NP_001038780.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Dgk3 phosphatidylinositol biosynthesis
    diacylglycerol more
    51(a)   43E18   --
    worm
    (Caenorhabditis elegans)
    Secernentea dgk-33 Diacylglycerol kinase 44(a)   III(9234802-9239752)   --


    ENSEMBL Gene Tree for DGKA (if available)
    TreeFam Gene Tree for DGKA (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for DGKA gene
    DGKH2  DGKQ2  DGKG2  DGKB2  DGKD2  DGKI2  DGKE2  DGKZ2  
    6 SIMAP similar genes for DGKA using alignment to 12 protein entries:     DGKA_HUMAN (see all proteins):
    DAGK1    DGKB    DGKG    DGKZ    DGKI    DGKE

    Find genes that share paralogs with DGKA           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for DGKA (see all 454)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1181646811,2
    F--56323795(+) AAGCTG/AAGTGC 1 -- us2k11Minor allele frequency- A:0.01NA 120
    rs1467466851,2
    --56323890(+) TCTTAC/TATTGT 4 -- us2k10--------
    rs1392069101,2
    C--56323912(+) AAGTAG/TCTCAG 4 -- us2k10--------
    rs1499667581,2
    --56323939(+) ACACAG/TTGCAC 4 -- us2k10--------
    rs573179281,2
    C--56324151(+) TTTTT-/GTTTTT 4 -- us2k10--------
    rs602618181,2
    C--56324154(+) TTTTT-/GTTTTT 4 -- us2k10--------
    rs597997031,2
    C--56324157(+) TTTTT-/GTTTTT 4 -- us2k10--------
    rs1809624391,2
    --56324273(+) AGCCTC/TGCGAG 4 -- us2k10--------
    rs111717021,2
    C--56324336(+) tagtaG/Aagaca 4 -- us2k11Minor allele frequency- A:0.50NA 2
    rs116153781,2
    H--56324368(+) gatggG/Tctcaa 4 -- us2k10--------

    HapMap Linkage Disequilibrium report for DGKA (56321103 - 56347811 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for DGKA:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv832424CNV Loss17160897

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing DGKA
    DNA2.0 Custom Variant and Variant Library Synthesis for DGKA

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 125855    OMIM disorders: --


    Find genes that share disorders with DGKA           About GenesLikeMe


    Export disorders for DGKA gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for DGKA gene, integrated from 10 sources (see all 63):
    (articles sorted by number of sources associating them with DGKA)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Assignment of the gene for diacylglycerol kinase (DAGK) to human chromosome 12. (PubMed id 8180475)1, 2, 3 Hart T.C.... van Dyke T.E. (Mamm. Genome 1994)
    2. Assignment of the human diacylglycerol kinase gene (DAGK) to 12q13.3 using fluorescence in situ hybridization analysis. (PubMed id 7959783)1, 2, 3 Hart T.C....Pettenati M.J. (Genomics 1994)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    4. Purification, cDNA-cloning and expression of human diacylglycerol kinase. (PubMed id 2175712)1, 2 Schaap D....van der Bendl R.L. (FEBS Lett. 1990)
    5. Lck-dependent tyrosine phosphorylation of diacylglycerol kinase alpha regulates its membrane association in T cells. (PubMed id 18424699)1, 9 Merino E....MAcrida I. (J. Immunol. 2008)
    6. Diacylglycerol kinase alpha regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes. (PubMed id 15870081)1, 9 Alonso R....Izquierdo M. (J. Biol. Chem. 2005)
    7. Diacylglycerol kinase-alpha phosphorylation by Src on Y335 is required for activation, membrane recruitment and Hgf-induced cell motility. (PubMed id 17700527)1, 9 Baldanzi G....Graziani A. (Oncogene 2008)
    8. Diacylglycerol kinase alpha enhances protein kinase Czeta-dependent phosphorylation at Ser311 of p65/RelA subunit of nuclear factor-kappaB. (PubMed id 19751727)1, 9 Kai M....Sakane F. (FEBS Lett. 2009)
    9. Activation of diacylglycerol kinase alpha is required for VEGF-induced angiogenic signaling in vitro. (PubMed id 15122338)1, 9 Baldanzi G....Graziani A. (Oncogene 2004)
    10. Src-mediated activation of alpha-diacylglycerol kinase is required for hepatocyte growth factor-induced cell motility. (PubMed id 10970854)1, 9 Cutrupi S....Graziani A. (EMBO J. 2000)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 1606 HGNC: 2849 AceView: DGKA Ensembl:ENSG00000065357 euGenes: HUgn1606
    ECgene: DGKA Kegg: 1606 H-InvDB: DGKA

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for DGKA Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for DGKA Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for DGKA gene:
    Search GeneIP for patents involving DGKA

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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