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Aliases for DGCR8 Gene

Aliases for DGCR8 Gene

  • DGCR8 Microprocessor Complex Subunit 2 3
  • DiGeorge Syndrome Critical Region Gene 8 2 3
  • DiGeorge Syndrome Critical Region 8 3 4
  • C22orf12 3 4
  • DGCRK6 3 4
  • Microprocessor Complex Subunit DGCR8 3
  • Chromosome 22 Open Reading Frame 12 2
  • Pasha 3
  • Gy1 3

External Ids for DGCR8 Gene

Previous HGNC Symbols for DGCR8 Gene

  • C22orf12

Previous GeneCards Identifiers for DGCR8 Gene

  • GC22P017008
  • GC22P018443
  • GC22P018444
  • GC22P018445
  • GC22P018446
  • GC22P020070
  • GC22P003688

Summaries for DGCR8 Gene

Entrez Gene Summary for DGCR8 Gene

  • This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

GeneCards Summary for DGCR8 Gene

DGCR8 (DGCR8 Microprocessor Complex Subunit) is a Protein Coding gene. Diseases associated with DGCR8 include digeorge syndrome. Among its related pathways are Translational Control and Gene Expression. GO annotations related to this gene include double-stranded RNA binding.

UniProtKB/Swiss-Prot for DGCR8 Gene

  • Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.

Gene Wiki entry for DGCR8 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DGCR8 Gene

Genomics for DGCR8 Gene

Regulatory Elements for DGCR8 Gene

Genomic Location for DGCR8 Gene

Start:
20,080,232 bp from pter
End:
20,111,877 bp from pter
Size:
31,646 bases
Orientation:
Plus strand

Genomic View for DGCR8 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for DGCR8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DGCR8 Gene

Proteins for DGCR8 Gene

  • Protein details for DGCR8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8WYQ5-DGCR8_HUMAN
    Recommended name:
    Microprocessor complex subunit DGCR8
    Protein Accession:
    Q8WYQ5
    Secondary Accessions:
    • B2R8G1
    • Q6DCB2
    • Q6MZE9
    • Q6Y2L0
    • Q96G39
    • Q96GP8
    • Q9H6L8
    • Q9H6T7
    • Q9NRW2

    Protein attributes for DGCR8 Gene

    Size:
    773 amino acids
    Molecular mass:
    86045 Da
    Cofactor:
    Name=heme; Xref=ChEBI:CHEBI:30413; Note=Binds 1 heme group per homodimer.;
    Quaternary structure:
    • Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization. Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DGCR8 (heme-free or heme-bound forms) and DROSHA. The microprocessor complex may contain multiple subunit of DGCR8 and DROSHA. Interacts with ILF3, NCL and DROSHA.
    SequenceCaution:
    • Sequence=AAO86726.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB15165.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB15238.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for DGCR8 Gene

    Alternative splice isoforms for DGCR8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DGCR8 Gene

Proteomics data for DGCR8 Gene at MOPED

Post-translational modifications for DGCR8 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for DGCR8 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for DGCR8 (DGCR8)

No data available for DME Specific Peptides for DGCR8 Gene

Domains for DGCR8 Gene

Protein Domains for DGCR8 Gene

Suggested Antigen Peptide Sequences for DGCR8 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q8WYQ5

UniProtKB/Swiss-Prot:

DGCR8_HUMAN :
  • Q8WYQ5
Domain:
  • Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has an autoinhibitory function on pri-miRNA processing activity
  • Contains 2 DRBM (double-stranded RNA-binding) domains.
  • Contains 1 WW domain.
genes like me logo Genes that share domains with DGCR8: view

No data available for Gene Families for DGCR8 Gene

Function for DGCR8 Gene

Molecular function for DGCR8 Gene

UniProtKB/Swiss-Prot Function: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.

Gene Ontology (GO) - Molecular Function for DGCR8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003725 double-stranded RNA binding IDA 17704815
GO:0004525 contributes_to ribonuclease III activity IDA 15574589
GO:0005515 protein binding IPI 15574589
GO:0020037 heme binding IDA 24449907
GO:0042803 protein homodimerization activity IDA 15574589
genes like me logo Genes that share ontologies with DGCR8: view
genes like me logo Genes that share phenotypes with DGCR8: view

Animal Models for DGCR8 Gene

MGI Knock Outs for DGCR8:

Animal Model Products

CRISPR Products

miRNA for DGCR8 Gene

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for DGCR8 Gene

Localization for DGCR8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DGCR8 Gene

Nucleus. Nucleus, nucleolus. Note=Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for DGCR8 Gene COMPARTMENTS Subcellular localization image for DGCR8 gene
Compartment Confidence
nucleus 5
cytoskeleton 4
cytosol 3

Gene Ontology (GO) - Cellular Components for DGCR8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 15574589
GO:0005654 nucleoplasm TAS --
GO:0005730 nucleolus IEA --
GO:0005737 cytoplasm IDA --
GO:0015630 microtubule cytoskeleton IDA --
genes like me logo Genes that share ontologies with DGCR8: view

Pathways for DGCR8 Gene

genes like me logo Genes that share pathways with DGCR8: view

Pathways by source for DGCR8 Gene

1 Cell Signaling Technology pathway for DGCR8 Gene
1 BioSystems pathway for DGCR8 Gene

Gene Ontology (GO) - Biological Process for DGCR8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0010467 gene expression TAS --
GO:0031053 primary miRNA processing IDA 15531877
GO:0072091 regulation of stem cell proliferation IEA --
GO:0090501 RNA phosphodiester bond hydrolysis IDA 15574589
GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic IDA 15574589
genes like me logo Genes that share ontologies with DGCR8: view

Transcripts for DGCR8 Gene

Unigene Clusters for DGCR8 Gene

DiGeorge syndrome critical region gene 8:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for DGCR8

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for DGCR8

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for DGCR8 Gene

ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b · 11c · 11d ^ 12a · 12b ^ 13a · 13b ^ 14a · 14b · 14c
SP1: - - - - - -
SP2: - - - - - - -
SP3: - - - - -
SP4: - - - - -
SP5: -
SP6: -
SP7: - -
SP8:

Relevant External Links for DGCR8 Gene

GeneLoc Exon Structure for
DGCR8
ECgene alternative splicing isoforms for
DGCR8

Expression for DGCR8 Gene

mRNA expression in normal human tissues for DGCR8 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for DGCR8 Gene

SOURCE GeneReport for Unigene cluster for DGCR8 Gene Hs.643452

mRNA Expression by UniProt/SwissProt for DGCR8 Gene

Q8WYQ5-DGCR8_HUMAN
Tissue specificity: Ubiquitously expressed.
genes like me logo Genes that share expressions with DGCR8: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for DGCR8 Gene

Orthologs for DGCR8 Gene

This gene was present in the common ancestor of animals.

Orthologs for DGCR8 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia DGCR8 35
  • 99.74 (n)
  • 100 (a)
ptr-mir-1306 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia bta-mir-1306 36
  • 93 (a)
OneToOne
DGCR8 35
  • 87.24 (n)
  • 92.24 (a)
dog
(Canis familiaris)
Mammalia cfa-mir-1306 36
  • 96 (a)
OneToOne
DGCR8 35
  • 92.41 (n)
  • 96.38 (a)
mouse
(Mus musculus)
Mammalia Dgcr8 35
  • 90.56 (n)
  • 95.34 (a)
Dgcr8 16
Dgcr8 36
  • 95 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia DGCR8 36
  • 82 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia DGCR8 36
  • 92 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Dgcr8 35
  • 90.51 (n)
  • 94.57 (a)
chicken
(Gallus gallus)
Aves DGCR8 35
  • 79.06 (n)
  • 84.7 (a)
gga-mir-1306 36
  • 84 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia aca-mir-5417 36
  • 82 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.19878 35
tropical clawed frog
(Silurana tropicalis)
Amphibia dgcr8 35
  • 72.87 (n)
  • 78.28 (a)
Str.18110 35
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.7506 35
zebrafish
(Danio rerio)
Actinopterygii dgcr8 35
  • 71.08 (n)
  • 74.83 (a)
dgcr8 36
  • 70 (a)
OneToOne
wufc23f08 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002554 35
  • 53.07 (n)
  • 45.02 (a)
fruit fly
(Drosophila melanogaster)
Insecta pasha 35
  • 49.94 (n)
  • 41.35 (a)
pasha 36
  • 36 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea pash-1 36
  • 22 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 41 (a)
OneToOne
Species with no ortholog for DGCR8:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DGCR8 Gene

ENSEMBL:
Gene Tree for DGCR8 (if available)
TreeFam:
Gene Tree for DGCR8 (if available)

Paralogs for DGCR8 Gene

Selected SIMAP similar genes for DGCR8 Gene using alignment to 3 proteins:

genes like me logo Genes that share paralogs with DGCR8: view

No data available for Paralogs for DGCR8 Gene

Variants for DGCR8 Gene

Sequence variations from dbSNP and Humsavar for DGCR8 Gene

SNP ID Clin Chr 22 pos Sequence Context AA Info Type MAF
rs174885 -- 20,078,328(+) gccTA(A/T)TTTTT upstream-variant-2KB
rs174886 -- 20,080,136(+) GACAC(A/G)CGCGC upstream-variant-2KB
rs174887 -- 20,080,172(+) GCAGC(C/G)CGCTC upstream-variant-2KB
rs174889 -- 20,095,737(+) tttct(A/G)ttatc intron-variant
rs174890 -- 20,098,156(+) aaaaa(A/T)ATATA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DGCR8 Gene

Variant ID Type Subtype PubMed ID
nsv435744 CNV Deletion 17901297
nsv828938 CNV Gain 20364138
nsv828939 CNV Loss 20364138
dgv72n68 CNV Loss 17160897
nsv914327 CNV Loss 21882294
nsv914330 CNV Gain 21882294
nsv471183 CNV Loss 18288195
nsv834133 CNV Loss 17160897
dgv1387e1 CNV Complex 17122850
nsv521042 CNV Loss 19592680
dgv726n67 CNV Gain 20364138
dgv4855n71 CNV Loss 21882294
dgv4856n71 CNV Loss 21882294
dgv4857n71 CNV Loss 21882294
dgv4858n71 CNV Loss 21882294
dgv4859n71 CNV Loss 21882294
dgv4860n71 CNV Loss 21882294
nsv914347 CNV Loss 21882294

Relevant External Links for DGCR8 Gene

HapMap Linkage Disequilibrium report
DGCR8
Human Gene Mutation Database (HGMD)
DGCR8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DGCR8 Gene

Disorders for DGCR8 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for DGCR8 Gene including...

Search for DGCR8 Gene in MalaCards »

(1) University of Copenhagen DISEASES for DGCR8 Gene

(1) Novoseek inferred disease relationships for DGCR8 Gene

Disease -log(P) Hits PubMed IDs
digeorge syndrome 87.8 5

Relevant External Links for DGCR8

Genetic Association Database (GAD)
DGCR8
Human Genome Epidemiology (HuGE) Navigator
DGCR8
genes like me logo Genes that share disorders with DGCR8: view

No data available for UniProtKB/Swiss-Prot for DGCR8 Gene

Publications for DGCR8 Gene

  1. Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma. (PMID: 19047128) Horikawa Y. … Wu X. (Clin. Cancer Res. 2008) 3 23 48
  2. Posttranscriptional crossregulation between Drosha and DGCR8. (PMID: 19135890) Han J. … Kim V.N. (Cell 2009) 3 4 23
  3. Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region. (PMID: 12705904) Shiohama A. … Shimizu N. (Biochem. Biophys. Res. Commun. 2003) 3 4 23
  4. The microprocessor complex mediates the genesis of microRNAs. (PMID: 15531877) Gregory R.I. … Shiekhattar R. (Nature 2004) 3 4 23
  5. Formation of GW bodies is a consequence of microRNA genesis. (PMID: 16906129) Pauley K.M. … Chan E.K.L. (EMBO Rep. 2006) 4 23

Products for DGCR8 Gene

Sources for DGCR8 Gene

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