Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

DGCR8 Gene

protein-coding   GIFtS: 57
GCID: GC22P020070

DiGeorge syndrome critical region gene 8

(Previous name: chromosome 22 open reading frame 12 )
(Previous symbol: C22orf12)
 Explore 8 diseases affiliated with
DGCR8 via
MalaCards
our new
 Human Malady Compendium 
Biological research products
for DGCR8    

Aliases
for DGCR8 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
DiGeorge Syndrome Critical Region Gene 81 2     Pasha1
C22orf121 2 3     DiGeorge Syndrome Critical Region 82 3
DGCRK61 2 3     Chromosome 22 Open Reading Frame 121
Gy11 2     Microprocessor Complex Subunit DGCR82

External Ids:    HGNC: 28471   Entrez Gene: 544872   Ensembl: ENSG000001281917   OMIM: 6090305   UniProtKB: Q8WYQ53   

Export aliases for DGCR8 gene to outside databases

Previous GC identifers: GC22P017008 GC22P018443 GC22P018444 GC22P018445 GC22P018446 GC22P003688


Summaries
for DGCR8 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for DGCR8:
This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary
microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic
subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and
facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple
transcript variants. (provided by RefSeq, Jun 2010)

UniProtKB/Swiss-Prot: DGCR8_HUMAN, Q8WYQ5
Function: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the
initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process
primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the
microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA
junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are
subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a
cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer
binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA
are required for its binding. Involved in the silencing of embryonic stem cells self-renewal

Gene Wiki entry for DGCR8


Genomic Views
for DGCR8 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000022.10  NC_018933.1  NT_011519.10  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the DGCR8 gene promoter:
         c-Fos   AP-1   MyoD   AREB6   CREB   RORalpha1   deltaCREB   c-Myb   c-Jun   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: DGCR8 promoter sequence
   Search SABiosciences Chromatin IP Primers for DGCR8

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DGCR8


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 22q11.2   Ensembl cytogenetic band:  22q11.21   HGNC cytogenetic band: 22q11.2

DGCR8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DGCR8 gene location

GeneLoc information about chromosome 22         GeneLoc Exon Structure

GeneLoc location for GC22P020070:  view genomic region     (about GC identifiers)

Start:
 20,067,755 bp from pter      End:
 20,099,400 bp from pter
Size:
 31,646 bases      Orientation:
 plus strand

Proteins
for DGCR8 gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: DGCR8_HUMAN, Q8WYQ5 (See protein sequence)
Recommended Name: Microprocessor complex subunit DGCR8  
Size: 773 amino acids; 86045 Da
Cofactor: Binds 1 heme group per homodimer
Subunit: Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization. Component of the
microprocessor complex, or pri-miRNA processing protein complex, which is composed of DGCR8 (heme-free or heme-bound
forms) and DROSHA. The microprocessor complex may contain multiple subunit of DGCR8 and DROSHA. Interacts with ILF3,
NCL and DROSHA
Subcellular location: Nucleus. Nucleus, nucleolus. Note=Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly
detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component
(GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure.
Localized with DROSHA in GW bodies (GWBs), also known as P-bodies
Sequence caution: Sequence=AAO86726.1; Type=Erroneous initiation; Sequence=BAB15165.1; Type=Erroneous initiation;
Sequence=BAB15238.1; Type=Erroneous initiation;
3 PDB 3D structures from and Proteopedia for DGCR8:
1X47 (3D)        2YT4 (3D)        3LE4 (3D)    
Secondary accessions: B2R8G1 Q6DCB2 Q6MZE9 Q6Y2L0 Q96G39 Q96GP8 Q9H6L8 Q9H6T7 Q9NRW2
Alternative splicing: 3 isoforms:  Q8WYQ5-1   Q8WYQ5-2   Q8WYQ5-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for DGCR8: NX_Q8WYQ5

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q8WYQ5

    • DGCR8 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001177255.1  NP_073557.3  

    ENSEMBL proteins: 
     ENSP00000263209   ENSP00000409625   ENSP00000384726   ENSP00000372488  
    Reactome Protein details: Q8WYQ5
    Human Recombinant Protein Products: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate: DGCR8
    OriGene Custom Protein Services for DGCR8 
    GenScript Custom Purified and Recombinant Proteins Services for DGCR8
    Novus Biologicals DGCR8 Proteins
    Novus Biologicals DGCR8 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for DGCR8

    Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005730NOT nucleolus IDA--
    GO:0005737cytoplasm IDA--
    GO:0015630microtubule cytoskeleton IDA--


    DGCR8 for ontologies           About GeneDecksing



    DGCR8 Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Cell Signaling Technology (CST) Antibodies for DGCR8 
    OriGene Antibodies: DGCR8
    OriGene Custom Antibody Services for DGCR8 
    GenScript Custom Superior Antibodies Services for DGCR8
    Novus Biologicals DGCR8 Antibodies
    Abcam antibodies for DGCR8 
    Uscn Antibodies for DGCR8
    ThermoFisher Antibodies for DGCR8

    Assay Products for DGCR8: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Immunoassay Development
    Browse OriGene Fluorogenic Cell Assay Kits
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for DGCR8
    Browse Enzo Life Sciences for kits & assays
    Uscn ELISAs and CLIAs for DGCR8

    Protein Domains /
    Families
    for DGCR8 gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    DGCR8 for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR001159 Ds-RNA-bd
     IPR014720 dsRNA-bd-like_dom
     IPR001202 WW_Rsp5_WWP

    Graphical View of Domain Structure for InterPro Entry Q8WYQ5

    ProtoNet protein and cluster: Q8WYQ5

    1 Blocks protein family: IPB001159 Double-stranded RNA binding (DsRBD) domain

    UniProtKB/Swiss-Prot: DGCR8_HUMAN, Q8WYQ5
    Domain: Both DRBM domains are required for efficient binding to pri-miRNA. The region between residues 276 and 498 has
    an autoinhibitory function on pri-miRNA processing activity
    Similarity: Contains 2 DRBM (double-stranded RNA-binding) domains
    Similarity: Contains 1 WW domain


    Function
    for DGCR8 gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: DGCR8_HUMAN, Q8WYQ5
    Function: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the
    initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process
    primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the
    microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA
    junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are
    subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a
    cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer
    binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA
    are required for its binding. Involved in the silencing of embryonic stem cells self-renewal

    miRNA
    Products:            		 OriGene 3'-UTR Clone: DGCR8
    Browse MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat DGCR8
    8/19 QIAGEN miScript miRNA Assays for microRNAs that regulate DGCR8 (see all 19):
    hsa-miR-218-1* hsa-miR-132 hsa-miR-650 hsa-miR-548a-3p hsa-miR-9 hsa-miR-499-5p hsa-miR-149 hsa-miR-548x
    SwitchGear 3'UTR luciferase reporter plasmid: DGCR8 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for DGCR8 (see all 7)
    OriGene shRNA RFP: DGCR8
    OriGene siRNA: DGCR8
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat DGCR8

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for DGCR8

    Clone
    Products:             		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DGCR8 (see all 4)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DGCR8
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector (see all 2): DGCR8 (NM_022720)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DGCR8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DGCR8 

    Cell Line
    Products:             		GenScript Custom overexpressing Cell Line Services for DGCR8
    Search LifeMap BioReagents cell lines for DGCR8

    In Situ Assay
    Products:       
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DGCR8

    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003725double-stranded RNA binding IDA17704815
    GO:0005515protein binding IPI15574589
    GO:0046872metal ion binding IEA--


    DGCR8 for ontologies           About GeneDecksing


    Animal Models:
         Mouse knock-out Dgcr8tm1.2Blel for DGCR8
         6 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Dgcr8):
     behavior/neurological  cardiovascular system  hearing/vestibular/ear  mortality/aging  muscle 
     nervous system 

    DGCR8 for phenotypes           About GeneDecksing


    Pathways & Interactions
    for DGCR8 gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Regulatory RNA pathways
    		Regulatory RNA pathways1.00
    		MicroRNA (miRNA) Biogenesis0.88
    2Direct p53 effectors
    		Direct p53 effectors1.00
    3Translational Control
    		Translational Control1.00
    4Generic Transcription Pathway
    		Gene Expression0.46

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for DGCR8
        Translational Control

    1 BioSystems Pathway for DGCR8 
        Direct p53 effectors

    3        Reactome Pathways for DGCR8
        MicroRNA (miRNA) Biogenesis
    Regulatory RNA pathways
    Gene Expression



    DGCR8 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for DGCR8

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/30 Interacting proteins for DGCR8 (Q8WYQ51, 2, 3 ENSP000002632094) via UniProtKB, MINT, STRING, and/or I2D (see all 30)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DROSHAQ9NRR41, 2, 3, ENSP000003398454EBI-528411,EBI-528367 MINT-8329598 I2D: score=4 STRING: ENSP00000339845
    DDX5P178442, 3, ENSP000002257924MINT-8329598 I2D: score=1 STRING: ENSP00000225792
    DDX17Q928412, 3, ENSP000003800334MINT-8329598 I2D: score=1 STRING: ENSP00000380033
    RBPJQ063303, ENSP000003452064I2D: score=4 STRING: ENSP00000345206
    HNRNPRO433903, ENSP000003637454I2D: score=1 STRING: ENSP00000363745
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0010467gene expression TAS--
    GO:0031053primary miRNA processing IDA15574589


    DGCR8 for ontologies           About GeneDecksing



    Drugs & Compounds
    for DGCR8 gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for DGCR8
    Search CenterWatch for drugs/clinical trials and news about DGCR8 

    Transcripts
    for DGCR8 gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for DGCR8 gene (2 alternative transcripts): 
    NM_001190326.1  NM_022720.6  

    Unigene Cluster for DGCR8:

    DiGeorge syndrome critical region gene 8
    Hs.643452  [show with all ESTs]
    Unigene Representative Sequence: NM_022720
    10 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000351989(uc002zri.3) ENST00000457069 ENST00000495826 ENST00000407755
    ENST00000498171(uc002zrj.3) ENST00000491892 ENST00000495351 ENST00000485802
    ENST00000475941 ENST00000383024(uc010grz.3)

    miRNA
    Products:             		 OriGene 3'-UTR Clone: DGCR8
    Browse OriGene MicroRNA Expression Plasmids
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat DGCR8
    8/19 QIAGEN miScript miRNA Assays for microRNAs that regulate DGCR8 (see all 19):
    hsa-miR-218-1* hsa-miR-132 hsa-miR-650 hsa-miR-548a-3p hsa-miR-9 hsa-miR-499-5p hsa-miR-149 hsa-miR-548x
    SwitchGear 3'UTR luciferase reporter plasmid: DGCR8 3' UTR sequence
    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for DGCR8 (see all 7)
    OriGene shRNA RFP: DGCR8
    OriGene siRNA: DGCR8
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat DGCR8
    Clone
    Products:             		 OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DGCR8 (see all 4)
    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DGCR8
    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector (see all 2): DGCR8 (NM_022720)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DGCR8
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DGCR8 
    Primer
    Products:            		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DGCR8
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat DGCR8
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DGCR8
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DGCR8

    Additional cDNA sequence: 

    AK025427.1 AK025539.1 AK025780.1 AK222796.1 AK313357.1 AY189282.1 BC009323.2 BC009984.2 
    BC037564.1 BC078147.1 CR456356.1 

    18 DOTS entries:

    DT.314290  DT.95117995  DT.95220939  DT.100021366  DT.100777569  DT.100831751  DT.410798  DT.120644639 
    DT.95126904  DT.120644732  DT.86853097  DT.91981213  DT.100041842  DT.100640408  DT.100654498  DT.120644689 
    DT.92434104  DT.92434106 

    24/449 AceView cDNA sequences (see all 449):

    AF165527 BM480148 AW964989 AI350727 AK025780 AI269257 AA825558 BQ718744 
    CD631586 BC037564 BM809627 BX342517 CR602031 BQ227305 AI659240 BM271852 
    BF223125 BU517132 AI160881 AI906092 AA319727 BC078147 BM264488 AI929630 

    GeneLoc Exon Structure

    5/8 Alternative Splicing Database (ASD) splice patterns (SP) for DGCR8 (see all 8)    About this scheme

    ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b · 11c · 11d ^ 12a · 12b ^ 13a · 13b ^ 14a · 14b · 14c
    SP1:                          -     -                 -                                         -                             -           -                     
    SP2:                          -     -                 -                       -                 -                             -           -                     
    SP3:                                                  -                                         -                 -           -           -                     
    SP4:                                                                                            -           -     -           -           -                     
    SP5:                                                                                            -                                                               


    ECgene alternative splicing isoforms for DGCR8

    Expression
    for DGCR8 gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    DGCR8 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CTTCAAGGCC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    DGCR8 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    KidneyCap MesenchymeCap Mesenchyme CellsKidney
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See DGCR8 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for DGCR8

    SOURCE GeneReport for Unigene cluster: Hs.643452

    UniProtKB/Swiss-Prot: DGCR8_HUMAN, Q8WYQ5
    Tissue specificity: Ubiquitously expressed

        SABiosciences Custom PCR Arrays for DGCR8
    Primer
    Products:    		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DGCR8
    Browse OriGene validated miRNA SYBR primer pairs
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat DGCR8
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DGCR8
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DGCR8
    In Situ
    Assay Products:     
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DGCR8

    Orthologs
    for DGCR8 gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of animals.

    Orthologs for DGCR8 gene from 7/22 species (see all 22)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    mouse
    (Mus musculus)    		 Mammalia Dgcr81 , 5 DiGeorge syndrome critical region gene 81, 5 90.56(n)1
    95.34(a)1    		   16 (11.31 cM)5
    942231  NM_033324.21  NP_201581.21 
     182539485 
    chicken
    (Gallus gallus)    		 Aves DGCR81 DiGeorge syndrome critical region gene 8 79.06(n)
    84.7(a)    		   416785  XM_415079.3  XP_415079.2 
    lizard
    (Anolis carolinensis)    		 Reptilia DGCR86
    		 --
    		 83(a)
    		 1 ↔ 1
    		 GL343364.1(1058390-1070888)
    African clawed frog
    (Xenopus laevis)    		 Amphibia Xl.198782 Xenopus laevis transcribed sequence with weak similarity more 77.91(n)    BQ400189.1 
    zebrafish
    (Danio rerio)    		 Actinopterygii wufc23f082 wufc23f08 81.18(n)   324284  AL721594.1 
    fruit fly
    (Drosophila melanogaster)    		 Insecta pasha1 partner of drosha 49.17(n)
    39.63(a)    		   43728  NM_143622.2  NP_651879.1 
    worm
    (Caenorhabditis elegans)    		 Secernentea pash-11 Protein PASH-1 43.51(n)
    32.63(a)    		   172950  NM_060373.4  NP_492774.1 


    ENSEMBL Gene Tree for DGCR8 (if available)
    TreeFam Gene Tree for DGCR8 (if available) 

    Paralogs
    for DGCR8 gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    		  --

    Genomic Variants
    for DGCR8 gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/568 NCBI SNPs in DGCR8 are shown (see all 568)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 22 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs1450983471,2
    		--20065793(+) ACCCAA/CCCGCC 2 -- us2k10--------
    rs1902158221,2
    		--20065815(+) AAGTGC/TTGGGA 2 -- us2k10--------
    rs1748851,2
    		C,--20065851(+) GCCTAT/ATTTTT 2 -- us2k19Minor allele frequency- A:0.00NA WA CSA EA 253
    rs1483440711,2
    		--20065937(+) CACTGC/TAACTC 2 -- us2k10--------
    rs2001217161,2
    		--20065977(+) TTCAG-/CCTCCTCC 2 -- us2k10--------
    rs1506482531,2
    		C,--20065982(+) CCTCC-/ACAGTAGC 2 -- us2k10--------
    rs1488399411,2
    		--20066136(+) TTACAC/GGCATG 2 -- us2k10--------
    rs1505261361,2
    		--20066165(+) TGCTCA/GCTATT 2 -- us2k10--------
    rs1383850411,2
    		--20066220(+) TGGCCC/TGATCT 2 -- us2k10--------
    rs1112755921,2
    		C,F,--20066241(+) CAACCT/ACCACC 2 -- us2k12Minor allele frequency- A:0.50WA CSA 4

    HapMap Linkage Disequilibrium report for DGCR8 (20067755 - 20099400 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 3 variations for DGCR8
         3 CNVs: 4117 5168 31071

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DGCR8
    DNA2.0 Custom Variant and Variant Library Synthesis for DGCR8

    Disorders / Diseases
    for DGCR8 gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    DGCR8 for disorders           About GeneDecksing

    OMIM gene information: 609030    OMIM disorders: --

    8 diseases for DGCR8:    About MalaCards
    digeorge syndrome    renal cell carcinoma    esophageal cancer    esophagitis
    colon cancer    lung cancer    schizophrenia    carcinoma

    1 disease from the University of Copenhagen DISEASES database for DGCR8:
    DiGeorge syndrome

    1 Novoseek disease relationship for DGCR8 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    digeorge syndrome 87.8 6 15867338 (1), 19301657 (1), 16957365 (1), 15531877 (1) (see all 5)

    Human Genome Epidemiology (HuGE) Navigator: DGCR8 (8 documents)

    Export disorders for DGCR8 gene to outside databases

    Publications
    for DGCR8 gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for DGCR8 gene, integrated from 9 sources (see all 61):
    (articles sorted by number of sources associating them with DGCR8)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Posttranscriptional crossregulation between Drosha and DGCR8. (PubMed id 19135890)1, 2, 9 Han J.... Kim V.N. (2009)
    2. Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region. (PubMed id 12705904)1, 2, 9 Shiohama A.... Shimizu N. (2003)
    3. The Microprocessor complex mediates the genesis of microRNAs. (PubMed id 15531877)1, 2, 9 Gregory R.I....Shiekhattar R. (2004)
    4. DiGeorge critical region 8 (DGCR8) is a double-cystei ne-ligated heme protein. (PubMed id 21454614)1, 3 Barr I....Guo F. (2011)
    5. Nucleolar localization of DGCR8 and identification of eleven DGCR8- associated proteins. (PubMed id 17765891)1, 2 Shiohama A.... Shimizu N. (2007)
    6. Crystal structure of human DGCR8 core. (PubMed id 17704815)1, 2 Sohn S.Y.... Cho Y. (2007)
    7. Heme is involved in microRNA processing. (PubMed id 17159994)1, 2 Faller M.... Guo F. (2007)
    8. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. (PubMed id 16751099)1, 2 Han J.... Kim V.N. (2006)
    9. The Drosha-DGCR8 complex in primary microRNA processing. (PubMed id 15574589)1, 2 Han J.... Kim V.N. (2004)
    10. The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis. (PubMed id 15589161)1, 2 Landthaler M.... Tuschl T. (2004)

    External Searches for DGCR8 gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing DGCR8 gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 54487 HGNC: 2847 AceView: DGCR8andDKFZp761P1121 Ensembl:ENSG00000128191 euGenes: HUgn54487
    ECgene: DGCR8 H-InvDB: DGCR8

    Other Databases showing DGCR8 gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing DGCR8 gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for DGCR8 Pharmacogenomics, SNPs, Pathways

    Intellectual Property
    for DGCR8 gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for DGCR8 gene:
    Search GeneIP for patents involving DGCR8

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
    for DGCR8 gene

    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Phosphatase Activity Assays/Reagents   Browse Recombinant/Natural Proteins  
     OriGene Antibodies for DGCR8   OriGene shRNA RFP for DGCR8  
     OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for DGCR8   OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DGCR8  
     OriGene Protein Over-expression Lysate for DGCR8   Browse OriGene Fluorogenic Cell Assay Kits  
     OriGene siRNA for DGCR8   OriGene 3'-UTR Clone for DGCR8  
     OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DGCR8   OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DGCR8  
     Browse OriGene GFP tagged cDNA clones in CMV expression vector   Browse OriGene MicroRNA Expression Plasmids  
     Browse OriGene basic RS shRNAs   Browse OriGene validated miRNA SYBR primer pairs  
     Browse OriGene full length recombinant human proteins expressed in human HEK293 cells   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for DGCR8   OriGene Custom Protein Services for DGCR8  
     OriGene Custom Immunoassay Development  

         		 
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat DGCR8
     QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DGCR8
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DGCR8
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat DGCR8
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DGCR8
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DGCR8
     GenScript Custom Purified and Recombinant Proteins Services for DGCR8 GenScript cDNA clones with any tag delivered in your preferred vector for DGCR8
     GenScript Custom Assay Services for DGCR8 GenScript Custom Superior Antibodies Services for DGCR8
     GenScript Custom overexpressing Cell Line Services for DGCR8 CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Antibodies & Assays for DGCR8 

     Regulatory tfbs in DGCR8 promoter
     Search Chromatin IP Primers for DGCR8
     RT2 qPCR Primer Assay in human, mouse, rat DGCR8
     GNC Network for DGCR8
     SABiosciences Custom PCR Arrays for DGCR8
     Search Tocris compounds for DGCR8
     Browse Sino Biological Proteins and Antibodies
     Browse Sino Biological cDNA Clones
     Antibodies/Proteins Production Services
     Rabbit Monoclonal Antibody Platform
     Bulk Purchasing
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     DGCR8 antibodies
     DGCR8 proteins
     DGCR8 lysates
     Antibodies for DGCR8
     See all of Abcam's Antibodies, Kits and Proteins for DGCR8
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins




     DGCR8 Proteins, Antibodies, CLIAs, and ELISAs
     Search LifeMap BioReagents cell lines for DGCR8
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DGCR8
     SwitchGear 3'UTR luciferase reporter plasmids for DGCR8
     SwitchGear Promoter luciferase reporter plasmids for DGCR8
     ThermoFisher Antibodies for DGCR8
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DGCR8
           
    GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 27 Apr 2013

    View Random Gene

    Category
    VWF
    (GIFTS: 73)
    von Willebrand factor
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 

    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Copyright © 1996-2013 , Weizmann Institute of Science. All Rights Reserved.
    Hot genes      Disease genes      DGCR8 gene at Home site.
    hostname: 356980-web2.xennexinc.com index build: 100 solr: 1.4