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DGAT1 Gene

protein-coding   GIFtS: 62
GCID: GC08M145539

Diacylglycerol O-Acyltransferase 1

(Previous name: diacylglycerol O-acyltransferase homolog 1 (mouse))
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Diacylglycerol O-Acyltransferase 11 2     Diacylglycerol O-Acyltransferase Homolog 1 (Mouse)1
Acyl-CoA Retinol O-Fatty-Acyltransferase2 3     ACAT Related Gene Product 12
Diglyceride Acyltransferase2 3     Acyl Coenzyme A:Cholesterol Acyltransferase Related Gene 12
Retinol O-Fatty-Acyltransferase2 3     Acyl-CoA:Diacylglycerol Acyltransferase2
ARAT2 3     AGRP13
DGAT2 3     EC 2.3.1.763
EC 2.3.1.203 8     ACAT-Related Gene Product 13
ARGP12 5     EC 2.3.18

External Ids:    HGNC: 28431   Entrez Gene: 86942   Ensembl: ENSG000001850007   OMIM: 6049005   UniProtKB: O759073   

Export aliases for DGAT1 gene to outside databases

Previous GC identifers: GC08M143202 GC08U900023 GC08M145142 GC08M145544 GC08M145510 GC08M140652


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for DGAT1 Gene:
This gene encodes an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein
catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer
acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases.
(provided by RefSeq, Jul 2013)

GeneCards Summary for DGAT1 Gene:
DGAT1 (diacylglycerol O-acyltransferase 1) is a protein-coding gene. Diseases associated with DGAT1 include congenital chronic diarrhea with protein-losing enteropathy, and protein-losing enteropathy. GO annotations related to this gene include retinol O-fatty-acyltransferase activity and transferase activity, transferring acyl groups. An important paralog of this gene is SOAT1.

UniProtKB/Swiss-Prot: DGAT1_HUMAN, O75907
Function: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and
fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL
(very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol.
Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid
homeostasis and prevent retinoid toxicity leading to skin and hair disorders

summary for DGAT1 Gene:
Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the generation of triglycerides from diacylglycerol
(DAG) and Acyl-CoA.




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000008.11  NC_018919.2  NT_008046.17  
Regulatory elements:
   Regulatory transcription factor binding sites in the DGAT1 gene promoter:
         PPAR-alpha   CREB   PPAR-gamma1   deltaCREB   PPAR-gamma2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidDGAT1 promoter sequence
   Search Chromatin IP Primers for DGAT1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat DGAT1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q24.3   Ensembl cytogenetic band:  8q24.3   HGNC cytogenetic band: 8q24.3

DGAT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DGAT1 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M145539:  view genomic region     (about GC identifiers)

Start:
145,538,247 bp from pter      End:
145,550,573 bp from pter
Size:
12,327 bases      Orientation:
minus strand

1 alternative location:
Chr8-,NW_003315924 83,843-96,163     

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: DGAT1_HUMAN, O75907 (See protein sequence)
Recommended Name: Diacylglycerol O-acyltransferase 1  
Size: 488 amino acids; 55278 Da
Subunit: Homodimer or homotetramer (By similarity)
Secondary accessions: B2RWQ2 D3DWL6 Q96BB8

Explore the universe of human proteins at neXtProt for DGAT1: NX_O75907

Explore proteomics data for DGAT1 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for DGAT1 (O75907) (see all 8)
     WNIPVHK  ESITPVGS  PLRMFRLWAFT  FHSCLNAVAEL 


    See DGAT1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_036211.2  
    ENSEMBL proteins: 
     ENSP00000332258   ENSP00000432795  
    Reactome Protein details: O75907

    DGAT1 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
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    OriGene Protein Over-expression Lysate for DGAT1
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    Cloud-Clone Corp. Proteins for DGAT1

    DGAT1 Antibody Products:

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    Abcam antibodies for DGAT1
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    LSBio Antibodies in human, mouse, rat for DGAT1

    DGAT1 Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    GenScript Custom Assay Services for DGAT1
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    Cloud-Clone Corp. ELISAs for DGAT1
    Cloud-Clone Corp. CLIAs for DGAT1


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    3 InterPro protein domains:
     IPR014371 Oat_ACAT_DAG_ARE
     IPR027251 Diacylglycerol_acylTrfase1
     IPR004299 MBOAT_fam

    Graphical View of Domain Structure for InterPro Entry O75907

    ProtoNet protein and cluster: O75907

    1 Blocks protein domain: IPB004299 Membrane bound O-acyl transferase

    UniProtKB/Swiss-Prot: DGAT1_HUMAN, O75907
    Similarity: Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily


    DGAT1 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: DGAT1_HUMAN, O75907
    Function: Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and
    fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL
    (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol.
    Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid
    homeostasis and prevent retinoid toxicity leading to skin and hair disorders
    Catalytic activity: Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol
    Catalytic activity: Acyl-CoA + retinol = CoA + retinyl ester
    Enzyme regulation: XP620 is a selective DGAT1 inhibitor
    Biophysicochemical properties: Kinetic parameters: KM=25.9 uM for retinol; KM=13.9 uM for palmitoyl coenzyme A;

         Enzyme Numbers (IUBMB): EC 2.3.1.201 2 EC 2.3.12 EC 2.3.1.761

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:00038462-acylglycerol O-acyltransferase activity IEA--
    GO:0004144diacylglycerol O-acyltransferase activity EXP--
    GO:0005515protein binding IPI--
    GO:0008374O-acyltransferase activity ----
    GO:0016746transferase activity, transferring acyl groups TAS9756920
         
    DGAT1 for ontologies           About GeneDecksing


    Phenotypes:
         8 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Dgat1):
     adipose tissue  behavior/neurological  digestive/alimentary  endocrine/exocrine gland  growth/size/body 
     homeostasis/metabolism  integument  liver/biliary system 

    DGAT1 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for DGAT1: Dgat1tm1Far Dgat1tm1Ryyo

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for DGAT1
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for DGAT1

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for DGAT1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for DGAT1

    miRNA
    Products:
        
    miRTarBase miRNAs that target DGAT1:
    hsa-mir-183-5p (MIRT047045), hsa-mir-484 (MIRT042201), hsa-mir-125b-5p (MIRT006524), hsa-mir-26b-5p (MIRT029044)

    Block miRNA regulation of human, mouse, rat DGAT1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate DGAT1 (see all 11):
    hsa-miR-1290 hsa-miR-1206 hsa-miR-125a-5p hsa-miR-876-5p hsa-miR-486-3p hsa-miR-3167 hsa-miR-125b hsa-miR-514
    SwitchGear 3'UTR luciferase reporter plasmidDGAT1 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for DGAT1
    Predesigned siRNA for gene silencing in human, mouse, rat DGAT1

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for DGAT1

    Clone
    Products:
         
    OriGene clones in human, mouse for DGAT1 (see all 7)
    OriGene ORF clones in mouse, rat for DGAT1
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: DGAT1 (NM_012079)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DGAT1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DGAT1

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for DGAT1
    Browse ESI BIO Cell Lines and PureStem Progenitors for DGAT1 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DGAT1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    DGAT1_HUMAN, O75907: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    endoplasmic reticulum5
    plasma membrane3
    extracellular2
    peroxisome2
    cytosol1
    mitochondrion1

    Gene Ontology (GO): 4 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005789endoplasmic reticulum membrane TAS--
    GO:0016020membrane ----
    GO:0016021integral component of membrane ISS--
    GO:0043231intracellular membrane-bounded organelle ----

    DGAT1 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for DGAT1 About   (see all 9)  
    See pathways by source

    SuperPathContained pathways About
    1Metabolism
    Metabolism0.38
    Metabolism of lipids and lipoproteins0.37
    Metabolic pathways0.38
    2Statin Pathway
    Statin Pathway0.73
    Statin Pathway, Pharmacodynamics0.73
    3triacylglycerol biosynthesis
    triacylglycerol biosynthesis0.48
    Triacylglyceride Synthesis0.36
    4Glycerolipid metabolism
    Glycerolipid metabolism
    Acyl chain remodeling of DAG and TAG0.00
    5Glycerophospholipid biosynthesis
    Glycerophospholipid biosynthesis0.64
    Phospholipid metabolism0.64

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 GeneGo (Thomson Reuters) Pathway for DGAT1
        Retinol metabolism

    3 BioSystems Pathways for DGAT1
        Statin Pathway
    Triacylglyceride Synthesis
    triacylglycerol biosynthesis

    2 Reactome Pathways for DGAT1
        Triglyceride Biosynthesis
    Acyl chain remodeling of DAG and TAG

    1 PharmGKB Pathway for DGAT1
        Statin Pathway, Pharmacodynamics

    4 Kegg Pathways  (Kegg details for DGAT1):
        Glycerolipid metabolism
    Retinol metabolism
    Metabolic pathways
    Fat digestion and absorption

    UniProtKB/Swiss-Prot: DGAT1_HUMAN, O75907
    Pathway: Lipid metabolism; glycerolipid metabolism


    DGAT1 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Array including DGAT1: 

              PPAR Targets in human mouse rat

    Interactions:

        Search GeneGlobe Interaction Network for DGAT1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for DGAT1 (O759071 ENSP000003322584) via UniProtKB, MINT, STRING, and/or I2D (see all 63)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CELENSP000003611514STRING: ENSP00000361151
    CYP26A1ENSP000002243564STRING: ENSP00000224356
    DGKDENSP000002640574STRING: ENSP00000264057
    DGKQENSP000002738144STRING: ENSP00000273814
    DHRS3ENSP000003653974STRING: ENSP00000365397
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 13):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006641triglyceride metabolic process TAS9756920
    GO:0006644phospholipid metabolic process TAS--
    GO:0016310phosphorylation ----
    GO:0019432triglyceride biosynthetic process TAS--
    GO:0019915lipid storage ISS--

    DGAT1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Compounds for DGAT1 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    YM 750Acyl-CoA:cholesterol acyltransferase (ACAT) inhibitor[138046-43-2]
    VULM 1457Acyl-CoA: cholesterol acyltransferase (ACAT) inhibitor[228544-65-8]
    N1,N11-Diethylnorspermine tetrahydrochloride Spermine and spermidine acetyltransferase potentiator[121749-39-1]
    (R)-(+)-Etomoxir sodium saltCarnitine palmitoyltransferase I (CPT1) inhibitor[828934-41-4]
    A 922500Diacylglycerol acyltransferase 1 (DGAT-1) inhibitor[959122-11-3]

    Selected HMDB Compounds for DGAT1 (see all 1165)    About this table
    CompoundSynonyms CAS #PubMed Ids
    (2E)-Decenoyl-CoA(E)-S-2-decenoate Coenzyme A (see all 15)10018-95-8--
    (2E)-Dodecenoyl-CoA2-trans-Dodecenoyl-Coenzyme A (see all 4)1066-12-2--
    (2E)-Hexadecenoyl-CoAtrans-2-Hexadecenoyl-CoA (see all 4)4460-95-1--
    (2E)-Octenoyl-CoA(E)-S-2-octenoate Coenzyme A (see all 16)10018-94-7--
    (2E)-Tetradecenoyl-CoA(2E)-Tetradecenoyl-Coenzyme A (see all 8)38795-33-4--
    2-Methylbutyryl-CoA2-methylbutanoyl-CoA (see all 11)6712-02-3--
    2-Methylhexanoyl-CoA2-Methylhexanoyl-CoEnzyme A (see all 5)----
    3Z-dodecenoyl-CoAS-[2-[3-[[(2R)-4-[[[(2R,3R,5R)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] (Z)-dodec-3-enethioate (see all 4)2152-91-2--
    Acetyl-CoAS-Acetyl coenzyme A (see all 13)72-89-9--
    Acrylyl-CoAAcryloyl coenzyme A (see all 14)5776-58-9--

    Selected Novoseek inferred chemical compound relationships for DGAT1 gene (see all 16)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    triacylglycerol 85.4 47 14557275 (4), 18768481 (3), 16864897 (2), 15500922 (2) (see all 20)
    monoacylglycerol 60.6 14 18768481 (5), 20374941 (1)
    fatty acid 59.6 18 16130466 (2), 12923227 (2), 15500922 (1), 20126314 (1) (see all 12)
    acyl-coa 58.8 4 20374941 (2), 11672446 (1)
    lipid 52.8 25 15669762 (2), 16459663 (1), 19028692 (1), 15500922 (1) (see all 17)
    palmitate 45.7 4 10070036 (2), 15500922 (1)
    glycerol 3-phosphate 45.2 1 16864897 (1)
    oleic acid 42.6 4 18278045 (1), 15308631 (1)
    acetyl-coa 35.9 1 12888629 (1)
    phosphatidylcholine 26.3 4 14557275 (2)



    DGAT1 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for DGAT1 gene: 
    NM_012079.5  

    Unigene Cluster for DGAT1:

    Diacylglycerol O-acyltransferase 1
    Hs.521954  [show with all ESTs]
    Unigene Representative Sequence: NM_012079
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000527438 ENST00000526479 ENST00000332324(uc003zbv.3) ENST00000524965
    ENST00000528718 ENST00000527885 ENST00000524844 ENST00000531896 ENST00000525371

    miRNA
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    Block miRNA regulation of human, mouse, rat DGAT1 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate DGAT1 (see all 11):
    hsa-miR-1290 hsa-miR-1206 hsa-miR-125a-5p hsa-miR-876-5p hsa-miR-486-3p hsa-miR-3167 hsa-miR-125b hsa-miR-514
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    Inhib. RNA
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    Clone
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    GenScript: all cDNA clones in your preferred vector: DGAT1 (NM_012079)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DGAT1
    Primer
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    OriGene qPCR primer pairs and template standards for DGAT1
    OriGene qSTAR qPCR primer pairs in human, mouse for DGAT1
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat DGAT1
      QuantiTect SYBR Green Assays in human, mouse, rat DGAT1
      Search QuantiFast Probe-based Assays in human, mouse, rat DGAT1

    Additional mRNA sequence: 

    AB057815.1 AF059202.1 BC006263.2 BC015762.1 BC023565.1 BC106084.1 BC150649.1 

    12 DOTS entries:

    DT.415463  DT.100780987  DT.121492236  DT.86840605  DT.411156  DT.97848211  DT.100688235  DT.100780988 
    DT.40291106  DT.100028131  DT.95195890  DT.100812890 

    Selected AceView cDNA sequences (see all 208):

    CR619886 BM476315 AI370656 BF345504 BE271571 BX099763 CR624618 CK825344 
    CR622706 BQ058342 CB161855 BF345707 BM851470 AW015835 AI366194 BM755533 
    BU070233 BU680970 BM743182 BI907285 BX420014 AI367529 BC015762 BM739263 

    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for DGAT1    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14a · 14b ^ 15a · 15b ^ 16 ^ 17
    SP1:                                                                                                        -     -                     
    SP2:              -                                                                                                                     
    SP3:                                                                                                                                    
    SP4:                                                                                                                                    
    SP5:                                                                                                                                    


    ECgene alternative splicing isoforms for DGAT1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    DGAT1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TACGTCCACG
    DGAT1 Expression
    About this image


    DGAT1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 2) fully expand
     
     Adipose (Muscoskeletal System)
             Visceral White Adipose
     
     Blood (Hematopoietic System)
             Hematopoietic Stem Cells Hematopoietic Bone Marrow
    DGAT1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    DGAT1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.521954
        Pathway & Disease-focused RT2 Profiler PCR Array including DGAT1: 
              PPAR Targets in human mouse rat

    Primer
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    Search QuantiFast Probe-based Assays in human, mouse, rat DGAT1
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DGAT1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for DGAT1 gene from Selected species (see all 19)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Dgat11 , 5 diacylglycerol O-acyltransferase 11, 5 83.19(n)1
    85.47(a)1
      15 (35.99 cM)5
    133501  NM_010046.21  NP_034176.11 
     765020155 
    lizard
    (Anolis carolinensis)
    Reptilia DGAT16
    diacylglycerol O-acyltransferase 1
    77(a)
    1 ↔ 1
    1(259580488-259602614)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia dgat11 diacylglycerol O-acyltransferase 1 70.43(n)
    72.87(a)
      101730812  XM_004920022.1  XP_004920079.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufd36g112 wufd36g11 77.43(n)   325875  BC063970.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta mdy1 , 3 triacylglycerol biosynthesis sterol
    O-acyltransferase3
    midway1
    44(a)3
    51.69(n)1
    46.14(a)1
      448871  NM_206002.21  NP_995724.11 
    worm
    (Caenorhabditis elegans)
    Secernentea mboa-21 mboa-2 50.99(n)
    48.39(a)
      179540  NM_001269372.1  NP_001256301.1 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes ARE2(YNR019W)4 Acyl-CoA:sterol acyltransferase, isozyme of Are1p; more   --   14(665339-667267) 855753  NP_014416.1 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons TAG11 TAG1 47.89(n)
    40.69(a)
      816464  NM_127503.2  NP_179535.1 
    rice
    (Oryza sativa)
    Liliopsida Os05g01968001 Os05g0196800 47.33(n)
    39.69(a)
      4338045  NM_001061404.2  NP_001054869.2 


    ENSEMBL Gene Tree for DGAT1 (if available)
    TreeFam Gene Tree for DGAT1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for DGAT1 gene
    SOAT12  SOAT22  

    DGAT1 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for DGAT1 (see all 332)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 8 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs78170031,2
    C,F,A--145538917(+) CCCATC/TCCCCC 1 -- ut315Minor allele frequency- T:0.30NA WA 244
    rs1896166081,2
    --145539028(+) CAAGGG/TAGCCC 1 -- ut310--------
    rs1823061901,2
    --145539102(+) CCTGTA/GCAGGG 1 -- ut310--------
    rs1877201171,2
    --145539155(+) GGGGAC/TACCAG 1 -- ut310--------
    rs1478995181,2
    --145539249(+) CCTCCC/TGCTAC 1 -- ut310--------
    rs1405208861,2
    C--145539267(+) GGGGGC/GCCCAT 1 -- ut310--------
    rs1508092281,2
    C--145539410(+) AACCTC/TGTGGA 1 -- ut310--------
    rs1455680261,2
    --145539515(+) TGGACA/GTAGCC 1 -- ut310--------
    rs1926865181,2
    --145539531(+) GTACCA/GTAGCC 1 -- ut310--------
    rs1488930491,2
    --145539674(+) GTTGAC/TCATCC 1 -- ut310--------

    HapMap Linkage Disequilibrium report for DGAT1 (145538247 - 145550573 bp)

    Structural Variations
         Database of Genomic Variants (DGV) Selected variations for DGAT1 (see all 24):    About this table    
    Variant IDTypeSubtypePubMed ID
    dgv7962n71CNV Loss21882294
    nsv891841CNV Loss21882294
    dgv7960n71CNV Loss21882294
    nsv891846CNV Loss21882294
    dgv7955n71CNV Loss21882294
    nsv891817CNV Loss21882294
    dgv7957n71CNV Loss21882294
    nsv891807CNV Loss21882294
    dgv7958n71CNV Loss21882294
    dgv7940n71CNV Loss21882294

    Human Gene Mutation Database (HGMD): DGAT1
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing DGAT1
    DNA2.0 Custom Variant and Variant Library Synthesis for DGAT1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 604900    OMIM disorders: --

    15 diseases for DGAT1:    
    About MalaCards
    congenital chronic diarrhea with protein-losing enteropathy    protein-losing enteropathy    fatty liver disease    obesity
    insulin resistance    hepatoblastoma    liver disease    hepatitis c virus
    diarrhea    morbid obesity    hepatitis c    atherosclerosis
    hepatitis    malaria    alzheimer's disease


    DGAT1 for disorders           About GeneDecksing

    4 Novoseek inferred disease relationships for DGAT1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    obesity 54.8 20 16595853 (3), 11956242 (2), 15569818 (2), 17907060 (2) (see all 9)
    fatty liver 46.8 1 16595853 (1)
    insulin resistance 33.6 3 11956242 (2), 16595853 (1)
    insulin sensitivity 16.6 2 15224651 (1), 16595853 (1)

    Genetic Association Database (GAD): DGAT1
    Human Genome Epidemiology (HuGE) Navigator: DGAT1 (4 documents)

    Export disorders for DGAT1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for DGAT1 gene, integrated from 10 sources (see all 115):
    (articles sorted by number of sources associating them with DGAT1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of two human genes encoding acyl coenzyme A:cholesterol acyltransferase-related enzymes. (PubMed id 9756920)1, 2, 3 Oelkers P.... Sturley S.L. (J. Biol. Chem. 1998)
    2. Acyl coenzyme A dependent retinol esterification by acyl coenzyme A: diacylglycerol acyltransferase 1. (PubMed id 16214399)1, 2, 9 Orland M.D.... Cheng D. (Biochim. Biophys. Acta 2005)
    3. Role of the DGAT gene C79T single-nucleotide polymorphism in French obese subjects. (PubMed id 14569040)1, 4, 9 Coudreau S.K....ClAcment K. (Obes. Res. 2003)
    4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    5. DGAT1 promoter polymorphism associated with alterations in body mass index, high density lipoprotein levels and blood pressure in Turkish women. (PubMed id 12123490)1, 4 Ludwig E.H....Farese R.V. (Clin. Genet. 2002)
    6. Acylation of acylglycerols by acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1). Functional importance of DGAT1 in the intestinal fat absorption. (PubMed id 18768481)1, 9 Cheng D....Hussain M.M. (J. Biol. Chem. 2008)
    7. Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts. (PubMed id 14557275)1, 9 Bagnato C. and Igal R.A. (J. Biol. Chem. 2003)
    8. DGAT1 participates in the effect of HNF4A on hepatic secretion of triglyceride-rich lipoproteins. (PubMed id 20167659)1, 9 Krapivner S....van't Hooft F.M. (Arterioscler. Thromb. Vasc. Biol. 2010)
    9. Niacin noncompetitively inhibits DGAT2 but not DGAT1 activity in HepG2 cells. (PubMed id 15258194)1, 9 Ganji S.H....Kashyap M.L. (J. Lipid Res. 2004)
    10. Human acyl-CoA:diacylglycerol acyltransferase is a tetrameric protein. (PubMed id 11672446)1, 9 Cheng D....Young P.R. (Biochem. J. 2001)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 8694 HGNC: 2843 AceView: DGAT1 Ensembl:ENSG00000185000 euGenes: HUgn8694
    ECgene: DGAT1 Kegg: 8694 H-InvDB: DGAT1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for DGAT1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for DGAT1 gene:
    Search GeneIP for patents involving DGAT1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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