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Aliases for DFFB Gene

Aliases for DFFB Gene

  • DNA Fragmentation Factor, 40kDa, Beta Polypeptide (Caspase-Activated DNase) 2 3
  • DFF2 3 4 6
  • CAD 3 4 6
  • DNA Fragmentation Factor 40 KDa Subunit 3 4
  • Caspase-Activated Deoxyribonuclease 3 4
  • Caspase-Activated Nuclease 3 4
  • Caspase-Activated DNase 3 4
  • DFF-40 3 4
  • DFF40 3 4
  • CPAN 3 4
  • DNA Fragmentation Factor Subunit Beta 3
  • EC 3.-.-.- 4

External Ids for DFFB Gene

Summaries for DFFB Gene

Entrez Gene Summary for DFFB Gene

  • Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

GeneCards Summary for DFFB Gene

DFFB (DNA Fragmentation Factor, 40kDa, Beta Polypeptide (Caspase-Activated DNase)) is a Protein Coding gene. Among its related pathways are Integrated Pancreatic Cancer Pathway and Cell Cycle / Checkpoint Control. GO annotations related to this gene include enzyme binding and nicotinate phosphoribosyltransferase activity.

UniProtKB/Swiss-Prot for DFFB Gene

  • Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology

Gene Wiki entry for DFFB Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DFFB Gene

Genomics for DFFB Gene

Genomic Location for DFFB Gene

Start:
3,857,267 bp from pter
End:
3,885,429 bp from pter
Size:
28,163 bases
Orientation:
Plus strand

Genomic View for DFFB Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for DFFB Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DFFB Gene

Regulatory Elements for DFFB Gene

Proteins for DFFB Gene

  • Protein details for DFFB Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O76075-DFFB_HUMAN
    Recommended name:
    DNA fragmentation factor subunit beta
    Protein Accession:
    O76075
    Secondary Accessions:
    • O60521
    • Q5SR22
    • Q9BYI4
    • Q9BYI5
    • Q9BYI6

    Protein attributes for DFFB Gene

    Size:
    338 amino acids
    Molecular mass:
    39110 Da
    Quaternary structure:
    • Heterodimer of DFFA and DFFB. Interacts with HIST1H1A.

    Three dimensional structures from OCA and Proteopedia for DFFB Gene

    Alternative splice isoforms for DFFB Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DFFB Gene

Proteomics data for DFFB Gene at MOPED

Post-translational modifications for DFFB Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for DFFB Gene

Domains for DFFB Gene

Protein Domains for DFFB Gene

UniProtKB/Swiss-Prot:

DFFB_HUMAN
Domain:
  • Contains 1 CIDE-N domain.:
    • O76075
genes like me logo Genes that share domains with DFFB: view

No data available for Gene Families for DFFB Gene

Function for DFFB Gene

Molecular function for DFFB Gene

GENATLAS Biochemistry: DNA fragmentation factor,beta polypeptide,DNAse,activated by caspase 3,expressed in a limited number of tissues including pancreas,spleen,prostate,ovary,regulated by DFFA,involved in apoptosis
UniProtKB/Swiss-Prot EnzymeRegulation: Inhibited by DFFA (DFF45)
UniProtKB/Swiss-Prot Function: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology

Enzyme Numbers (IUBMB) for DFFB Gene

Gene Ontology (GO) - Molecular Function for DFFB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding --
GO:0004518 nuclease activity --
GO:0004536 deoxyribonuclease activity IBA --
GO:0005515 protein binding --
GO:0016787 hydrolase activity --
genes like me logo Genes that share ontologies with DFFB: view

Phenotypes for DFFB Gene

genes like me logo Genes that share phenotypes with DFFB: view

Animal Models for DFFB Gene

MGI Knock Outs for DFFB:

miRNA for DFFB Gene

miRTarBase miRNAs that target DFFB

No data available for Transcription Factor Targeting and HOMER Transcription for DFFB Gene

Localization for DFFB Gene

Subcellular locations from UniProtKB/Swiss-Prot for DFFB Gene

Cytoplasm. Nucleus.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for DFFB Gene COMPARTMENTS Subcellular localization image for DFFB gene
Compartment Confidence
nucleus 5
cytosol 4
mitochondrion 2
peroxisome 1

Gene Ontology (GO) - Cellular Components for DFFB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin IDA 19882353
GO:0005622 intracellular --
GO:0005634 nucleus IDA --
GO:0005654 nucleoplasm TAS --
GO:0005730 nucleolus IDA --
genes like me logo Genes that share ontologies with DFFB: view

Pathways for DFFB Gene

genes like me logo Genes that share pathways with DFFB: view

Gene Ontology (GO) - Biological Process for DFFB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006309 apoptotic DNA fragmentation TAS --
GO:0006915 apoptotic process TAS --
GO:0006921 cellular component disassembly involved in execution phase of apoptosis TAS --
GO:0007420 brain development --
GO:0012501 programmed cell death TAS --
genes like me logo Genes that share ontologies with DFFB: view

Compounds for DFFB Gene

(3) Novoseek inferred chemical compound relationships for DFFB Gene

Compound -log(P) Hits PubMed IDs
etoposide 1.67 1
atp 0 1
histidine 0 2
genes like me logo Genes that share compounds with DFFB: view

Transcripts for DFFB Gene

Unigene Clusters for DFFB Gene

DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for DFFB Gene

ExUns: 1 ^ 2a · 2b ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11
SP1: - - - -
SP2: - - - - -
SP3: - - - - - -
SP4: - - - - -
SP5: - - - - - -
SP6:
SP7:
SP8:

Relevant External Links for DFFB Gene

GeneLoc Exon Structure for
DFFB
ECgene alternative splicing isoforms for
DFFB

Expression for DFFB Gene

mRNA expression in normal human tissues for DFFB Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for DFFB Gene

SOURCE GeneReport for Unigene cluster for DFFB Gene Hs.133089

genes like me logo Genes that share expressions with DFFB: view

Orthologs for DFFB Gene

This gene was present in the common ancestor of animals.

Orthologs for DFFB Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia DFFB 36
  • 93.49 (n)
  • 92.01 (a)
DFFB 37
  • 98 (a)
OneToOne
cow
(Bos Taurus)
Mammalia DFFB 36
  • 78.83 (n)
  • 75.37 (a)
DFFB 37
  • 74 (a)
OneToOne
dog
(Canis familiaris)
Mammalia DFFB 36
  • 80.28 (n)
  • 78.11 (a)
DFFB 37
  • 77 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Dffb 36
  • 79.17 (n)
  • 77.68 (a)
Dffb 16
Dffb 37
  • 76 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia DFFB 37
  • 69 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia DFFB 37
  • 70 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Dffb 36
  • 79.9 (n)
  • 76.72 (a)
chicken
(Gallus gallus)
Aves DFFB 36
  • 65.95 (n)
  • 60.55 (a)
DFFB 37
  • 49 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia DFFB 37
  • 59 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia dffb 36
  • 62.5 (n)
  • 58.54 (a)
zebrafish
(Danio rerio)
Actinopterygii dffb 36
  • 59.37 (n)
  • 52.19 (a)
dffb 37
  • 50 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP009681 36
  • 46.79 (n)
  • 33.75 (a)
fruit fly
(Drosophila melanogaster)
Insecta Drep-4 36
  • 47.67 (n)
  • 36.01 (a)
Drep-4 37
  • 22 (a)
OneToOne
Species with no ortholog for DFFB:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for DFFB Gene

ENSEMBL:
Gene Tree for DFFB (if available)
TreeFam:
Gene Tree for DFFB (if available)

Paralogs for DFFB Gene

Pseudogenes.org Pseudogenes for DFFB Gene

genes like me logo Genes that share paralogs with DFFB: view

No data available for Paralogs for DFFB Gene

Variants for DFFB Gene

Sequence variations from dbSNP and Humsavar for DFFB Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs1141070 -- 3,869,625(+) TCCAC(A/G)GTGGG synonymous-codon, reference, nc-transcript-variant
rs3205087 -- 3,883,678(+) GACCC(A/G)AGCAG reference, synonymous-codon, nc-transcript-variant
rs4073323 -- 3,881,485(-) GGGCC(C/T)ACCAG intron-variant
rs4073324 -- 3,881,431(-) GGGAG(A/G)TGGGA intron-variant
rs4074709 -- 3,880,384(+) GAAAC(G/T)ATAAC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DFFB Gene

Variant ID Type Subtype PubMed ID
nsv871323 CNV Loss 21882294
nsv509590 CNV Insertion 20534489
esv1467252 CNV Insertion 17803354
esv1008825 CNV Deletion 20482838
esv1273516 CNV Deletion 17803354
esv992605 CNV Deletion 20482838
esv2730361 CNV Deletion 23290073
esv2730472 CNV Deletion 23290073

Relevant External Links for DFFB Gene

HapMap Linkage Disequilibrium report
DFFB

Disorders for DFFB Gene

(8) Novoseek inferred disease relationships for DFFB Gene

Disease -log(P) Hits PubMed IDs
ehrlich ascites tumor 40.9 2
huntington disease 24.6 2
cervical carcinoma 12.6 2
tumors 11 5
necrosis 9.51 5

Relevant External Links for DFFB

Genetic Association Database (GAD)
DFFB
Human Genome Epidemiology (HuGE) Navigator
DFFB
genes like me logo Genes that share disorders with DFFB: view

No data available for UniProtKB/Swiss-Prot for DFFB Gene

Publications for DFFB Gene

  1. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. (PMID: 9560346) Halenbeck R. … Williams L.T. (Curr. Biol. 1998) 2 3 4 23
  2. Molecular cloning and characterization of human caspase-activated DNase. (PMID: 9689044) Mukae N. … Nagata S. (Proc. Natl. Acad. Sci. U.S.A. 1998) 3 4 23
  3. Structure and mutation analysis of the gene encoding DNA fragmentation factor 40 (caspase-activated nuclease), a candidate neuroblastoma tumour suppressor gene. (PMID: 10830907) Judson H. … Bonthron D.T. (Hum. Genet. 2000) 3 4 23
  4. Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells. (PMID: 19882353) Ninios Y.P. … Sourlingas T.G. (Apoptosis 2010) 3 4 23
  5. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. (PMID: 9108473) Liu X. … Wang X. (Cell 1997) 2 3

Products for DFFB Gene

Sources for DFFB Gene

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