DET1 Gene
protein-coding GIFtS: 51
GCID: GC15M089055
|
|
de-etiolated homolog 1 (Arabidopsis)
| |
Aliases
for DET1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
This gene clusters with an RNA gene
Subcategory (RNA class): lncRNA
Quality score for the ORGUL clustered with this gene is 3
| Aliases |
|---|
| De-Etiolated Homolog 1 (Arabidopsis)1 2 | | De-Etiolated-1 Homolog2 3 | | FLJ101031 | | De-Etiolated 12 | | DET1 Homolog2 |
| External Ids: | HGNC: 254771 | Entrez Gene: 550702 | Ensembl: ENSG000001405437 | OMIM: 6087275 | UniProtKB: Q7L5Y63 |
|---|
| | ORGUL members: | |
|---|
| NONCODE:n407184 | |
Export aliases for DET1 gene to outside databasesPrevious GC identifers: GC15M086786 GC15M086856 GC15M065167
Summaries
for DET1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: DET1_HUMAN, Q7L5Y6Function: Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination andsubsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation
|
Genomic Views
for DET1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000015.9 NC_018926.1 NT_010274.17
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the DET1 gene promoter:
Egr-2 c-Fos Sp1 AP-1 ATF-2 c-Jun
Other transcription factors
Search SABiosciences Chromatin IP Primers for DET1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DET1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 15q25.3 Ensembl cytogenetic band: 15q25.3 HGNC cytogenetic band: 15q25.3DET1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 15 GeneLoc Exon Structure GeneLoc location for GC15M089055: view genomic region
(about GC identifiers)
Start:
|
89,054,790 bp from pter |
End:
|
89,089,912 bp from pter |
Size:
|
35,123 bases |
Orientation:
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minus strand |
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Proteins
for DET1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: DET1_HUMAN, Q7L5Y6 (See
protein sequence)Recommended Name: DET1 homolog Size: 550 amino acids; 63848 Da
Subunit: Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1
Subcellular location: Nucleus (Probable)
Sequence caution: Sequence=BAA91447.1; Type=Erroneous initiation;
Secondary accessions: B3KNN6 Q2VPC0 Q9NWD5Alternative splicing: 2 isoforms: Q7L5Y6-1 Q7L5Y6-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for DET1: NX_Q7L5Y6
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q7L5Y6
DET1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001137546.1 NP_060466.2
ENSEMBL proteins: ENSP00000452856 ENSP00000456340 ENSP00000452613 ENSP00000268148 ENSP00000453900 ENSP00000408994 Reactome Protein details: Q7L5Y6
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005634 | nucleus |
IEA | -- |
DET1 for ontologies About GeneDecksing
DET1 Antibody Products:
Assay Products for DET1: |
Protein
Domains /
Families
for DET1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
DET1 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q7L5Y6ProtoNet protein and cluster: Q7L5Y6 UniProtKB/Swiss-Prot: DET1_HUMAN, Q7L5Y6Similarity: Belongs to the DET1 family |
Function
for DET1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: DET1_HUMAN, Q7L5Y6Function: Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination andsubsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DET1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DET1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): DET1 (NM_001144074) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for DET1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DET1 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DET1 |
2 GenomeRNAi human phenotypes for DET1:
|
Pathways & Interactions
for DET1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Antigen processing: Ubiquitination & Proteasome degradation | | | 2 | Immune System | |
Pathway sources
See GeneCards unified pathways
Show all pathways
5 
Reactome Pathways for DET1
1 
Kegg Pathway (Kegg details for DET1):
DET1 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for DET1
STRING Interaction
Network Preview (showing 5 interactants - click image to see 11)
5/11 Interacting proteins for DET1 (Q7L5Y63 ENSP000004089944) via UniProtKB, MINT, STRING, and/or I2D (see all 11)About this table
|
Drugs & Compounds
for DET1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
DET1 for compounds About GeneDecksing
 Browse Tocris compounds for DET1
1 Novoseek chemical compound relationship for DET1 gene About this table
| Compound |
-log (P-Val) |
Hits |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| phytochrome |
73.9 |
1 |
1467650 (1) |
Search CenterWatch for drugs/clinical trials and news about DET1
|
Transcripts
for DET1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for DET1 gene (2 alternative transcripts): NM_001144074.1 NM_017996.3 Unigene Cluster for DET1: De-etiolated homolog 1 (Arabidopsis) Hs.690871 [show with all ESTs]Unigene Representative Sequence: NM_0179967 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000557842(uc002bmp.4 uc010bnk.2) ENST00000564406(uc002bmq.2)
ENST00000557837 ENST00000268148(uc002bmr.2) ENST00000558413 ENST00000559656
ENST00000444300
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DET1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DET1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): DET1 (NM_001144074) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for DET1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DET1 |
Additional cDNA sequence: AB073887.1 AK000965.1 AK054603.1 BC001242.2 BC064591.1 BC073867.1 BC109060.1 BC109061.1 NR_026645.1 7 DOTS entries: DT.121018570 DT.410562 DT.121018680 DT.40282523 DT.101958552 DT.92419372 DT.121018693 24/254 AceView cDNA sequences (see all 254): AI214969 AI433200 BG574987 AI188252 AA309872 AK054603 BQ005617 BU624057 CR615320 BQ929269 AA310699 AI355641 NM_017996 CF528691 CD674043 AI375053 BQ001942 CR609481 BE257656 BQ001929 AA128642 AA872336 AA683079 AI469869
GeneLoc Exon Structure
3 Alternative Splicing Database (ASD) splice patterns (SP) for DET1 About this scheme
| ExUns: | 1 | ^ | 2a | · | 2b | ^ | 3 | ^ | 4 |
|---|
|
| SP1: | | | | | | | | | | | |
| SP2: | | | | | | | | | | | |
| SP3: | | | | | | | | | | |
ECgene alternative splicing isoforms for DET1
|
Expression
for DET1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| DET1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See DET1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for DET1
SOURCE GeneReport for Unigene cluster: Hs.690871
UniProtKB/Swiss-Prot: DET1_HUMAN, Q7L5Y6Tissue specificity: Highly expressed in the ovary, some lymphoid organs and resting leukocytes
SABiosciences Expression via Pathway-Focused PCR Array including DET1:
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DET1
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat DET1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DET1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DET1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DET1 |
Orthologs
for DET1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for DET1 gene from 8/23 species (see all 23) About this table
ENSEMBL Gene Tree for DET1 (if available)
TreeFam Gene Tree for DET1 (if available) |
Paralogs
for DET1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for DET1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 15 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for DET1 (89054790 - 89089912 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for DET1: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DET1 |
|
Disorders
/ Diseases
for DET1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
 DET1 for disorders About MalaCards
DET1 for disorders About GeneDecksing
OMIM gene information: 608727
OMIM disorders: --
Export disorders for DET1 gene to outside databases
|
Publications
for DET1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for DET1 gene, integrated from 9 sources (see all 15):
(articles sorted by number of sources associating them with DET1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase. (PubMed id 14739464)1, 2, 3 Wertz I.E.... Dixit V.M. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Mammalian DET1 regulates Cul4A Activity and forms stable complexes with E2 ubiquitin-conjugating enzymes. (PubMed id 17452440)1, 9 Pick E.... Wei N. (2007)
- COP1 is a tumour suppressor that causes degradation o f ETS transcription factors. (PubMed id 21572435)1 Vitari A.C....Dixit V.M. (2011)
- An interaction network of the mammalian COP9 signalos ome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. (PubMed id 19295130)1 Olma M.H....Pintard L. (2009)
- A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1. (PubMed id 16949367)1 Jin J.... Walter J.C. (2006)
- Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (2006)
- Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery. (PubMed id 16964240)1 Angers S.... Zheng N. (2006)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
|
External Searches for DET1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing DET1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing DET1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing DET1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for DET1 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for DET1 gene:
Search GeneIP for patents involving DET1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for DET1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for DET1 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for DET1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DET1 | | | OriGene Protein Over-expression Lysate for DET1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | Browse OriGene siRNAs | | OriGene 3'-UTR Clone for DET1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DET1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DET1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for DET1 | | OriGene Custom Protein Services for DET1 | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat DET1 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DET1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DET1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat DET1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DET1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DET1 |
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 |  |  |  | |
| | | | Search Tocris compounds for DET1 |
| |  |  |  |  | | | | |
 |
 |
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 | | | DET1 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DET1 |
|  |  |  | | | | ThermoFisher Antibodies for DET1 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DET1 |
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