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DEDD2 Gene

protein-coding   GIFtS: 51

GC19M047394
death effector domain containing 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
FLAME-3 1, 2
FLAME3 3
Descriptions
DED-containing protein FLAME-3 3
FADD-like anti-apoptotic molecule 3 3
death effector domain containing 2 2
death effector domain-containing DNA binding protein 2 2
External Ids
HGNC: 244501
Entrez Gene: 1629892
UniProtKB: Q8WXF83
Ensembl: ENSG000001605707
Search outside databases for aliases for DEDD2 gene

Previous GC identifers: GC19M043343 GC19M043094 GC19M047378

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: DEDD2_HUMAN, Q8WXF8
Function: May play a critical role in death receptor-induced apoptosis and may target CASP8 and
CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May
play a role in the general transcription machinery in the nucleus and might be an important
regulator of the activity of GTF3C3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DEDD2 gene  

Entrez Gene cytogenetic band: 19q13.2   Ensembl cytogenetic band:  19q13.2   HGNC cytogenetic band: 19q13.31

DEDD2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M047394:     (about GC identifiers)

Start:
47,394,592 bp from pter
End:
47,416,115 bp from pter
Size:
21,524 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000019.8  NT_011109.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: DEDD2_HUMAN, Q8WXF8 (See protein sequence)
Recommended Name: DNA-binding death effector domain-containing protein 2  
Size: 326 amino acids; 36179 Da
Subunit: Interacts with CASP8, CASP10 and GTF3C3. Homodimerizes and heterodimerizes with DEDD
Subcellular location: Nucleus, nucleolus. Note=Nuclear, accumulated in subnuclear structures
resembling nucleoli
Sequence caution: Sequence=AAH13372.2; Type=Frameshift; Positions=186;
Secondary accessions: Q8NBR2 Q8NES1 Q8TAA8 Q96D35
Alternative splicing: 2 isoforms:  Q8WXF8-1   Q8WXF8-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_579874.1  

    ENSEMBL proteins: 
    ENSP00000384031 ENSP00000336972 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (DEDD2)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005730 nucleolus IDA11741985
    About this table

    Antibodies for DEDD2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for DEDD2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (DEDD2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (DEDD2)
    Novus Biologicals Antibodies for DEDD2

    Assays for DEDD2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR001875 DED


       GeneDecks  DEDD2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8WXF8

    ProtoNet protein and cluster: Q8WXF8

    1 Blocks protein family: IPB001875 Death effector protein

    UniProtKB/Swiss-Prot: DEDD2_HUMAN, Q8WXF8
    Domain: Interactions with CASP8 and CASP10 are mediated by the DED domain
    Similarity: Contains 1 DED (death effector) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DEDD2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DEDD2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_133328

                  Applied Biosystems Silencer® siRNAs for DEDD2

                  Sigma-Aldrich siRNA for DEDD2  
                         Sigma-Aldrich shRNA for DEDD2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for DEDD2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_133328
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_133328
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_133328 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_133328

    UniProtKB/Swiss-Prot: DEDD2_HUMAN, Q8WXF8
    Function: May play a critical role in death receptor-induced apoptosis and may target CASP8 and
    CASP10 to the nucleus. May regulate degradation of intermediate filaments during apoptosis. May
    play a role in the general transcription machinery in the nucleus and might be an important
    regulator of the activity of GTF3C3

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    GO:0005515 protein binding IPI11741985
    GO:0030159 receptor signaling complex scaffold activity NAS11741985
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    3 Invitrogen iPath™ Online BioAtlas - Pathways for DEDD2 (Maps provided by GeneGo):
     Caspase cascade activation by FADD and RIPK proteins
     Caspases activation via nuclear import
     FAS signaling cascades. Part 2

       GeneDecks  DEDD2 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  DEDD2 


    5/7 Interacting proteins for DEDD2 (ENSP000003369723) via UniProtKB, MINT, and/or STRING (see all 7 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CFLARENSP000003124553STRING (score=.991)
    AATFENSP000002254023STRING (score=.977)
    CASP8ENSP000003512733STRING (score=.957)
    DEDDENSP000003569843STRING (score=.926)
    GTF3C3ENSP000002639563STRING (score=.878)
    About this table

    5/10 Gene Ontology (GO) biological process terms (links to tree view) (see all 10 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0006396 RNA processing NAS11741985
    GO:0007242 intracellular signaling cascade NAS11741985
    GO:0008625 induction of apoptosis via death domain receptors IMP11741985
    GO:0008633 activation of pro-apoptotic gene products NAS11741985
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DEDD2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DEDD2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DEDD2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_133328

                  Sigma-Aldrich siRNA for DEDD2  
                         Sigma-Aldrich shRNA for DEDD2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_133328  

    REFSEQ mRNAs for DEDD2 gene: 

    NM_133328.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_133328  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_133328
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_133328
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_133328 

    Additional cDNA sequence: 

    AF443591.1 AF457575.1 AK075328.1 AK130203.1 AK307895.1 AY125488.1 BC013372.2 BC027930.1 
    CR590827.1 CR593642.1 CR599685.1 CR604307.1 CR619301.1 CR625070.1 

    11 DOTS entries:

    DT.92426460  DT.101972579  DT.100817959  DT.454872  DT.100817965  DT.86847429  DT.95290001  DT.95304008 
    DT.95304009  DT.100691056  DT.95304007 

    24/167 AceView cDNA sequences (see all 167 ):

    CR604307 AW263988 BM979999 CA416835 F35595 BG251151 BI907024 BI520263 
    BM928759 AK130203 BQ072022 BF665646 CR625070 AW270125 CR593642 CR590827 
    AI432940 BU191631 CR619301 BM670485 BM908148 BU682972 AK075328 BM672838 

    highest scoring ESTs for DEDD2:

    AA218681 AA662107 AK130203 AL562850 AY125488 BC013372 BC027930 BE242821 BG122215 BG393347 

    Unigene Cluster for DEDD2:

    Death effector domain containing 2
    Hs.515432  [show with all ESTs]
    Unigene Representative Sequence: AY125488


    GeneLoc Exon Structure

    5/13 Alternative Splicing Database (ASD) splice patterns (SP) for DEDD2 (see all 13 )

    ExUns: 1 ^ 2a · 2b · 2c · 2d · 2e · 2f · 2g ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10a · 10b · 10c · 10d · 10e · 10f
    SP1:                                            -     -     -                       -                       -                                       
    SP2:                                            -                             -                                                                     
    SP3:                                            -                             -     -                 -     -                                       
    SP4:                                            -     -     -                 -     -                       -                                       
    SP5:                                            -                             -                                                                     

    About this scheme

    ECgene alternative splicing isoforms for DEDD2

    2 Ensembl transcripts including schematic representations:
    ENST00000403271  ENST00000336034  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DEDD2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DEDD2

    1 / 2 / 3

    3 probe-sets matching DEDD2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    55612_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AA662107 0.80 1.00 0.91 1

    225434_at2, 3 U133-B 2 1.00 0.95 -- -- AW245401 0.60 1.00 0.82 1

    225434_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGGCCAAAAC

    SOURCE GeneReport for Unigene cluster: Hs.515432

    UniProtKB/Swiss-Prot: DEDD2_HUMAN, Q8WXF8
    Tissue specificity: Expressed in most tissues. High levels were found in liver, kidney, heart,
    ovary, spleen, testes, skeletal muscle and peripheral blood leukocytes. Expression was absent or
    low in colon and small intestine. Expression is relatively high in the tumor cell lines chronic
    myologenous leukemia K-562 and the colorectal adenocarcinoma SW480. Expression is moderate in the
    cervical carcinoma HeLa, the Burkitt's lymphoma Raji, the lung carcinoma A549, and the melanoma
    G361. In contrast, two leukemia cell lines, HL-60 (promyelocytic leukemia) and MOLT-4
    (lymphoblastic leukemia), show relatively low levels

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DEDD2 gene from 5 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DEDD21   -- death effector domain containing 2 91.21(n)
    95.09(a)
    613012  XM_850181.1  XP_855274.1 
    chimpanzee
    (Pan troglodytes)
    DEDD21   -- death effector domain containing 2 98.77(n)
    98.47(a)
    456403  XM_001153280.1  XP_001153280.1 
    cow
    (Bos taurus)
    DEDD21   -- death effector domain containing 2 89.47(n)
    94.17(a)
    534251  NM_001076017.1  NP_001069485.1 
    mouse
    (Mus musculus)
    Dedd21, 5 75
    death effector domain-containing DNA binding protein more1, 5 87.53(n)1
    93.25(a)1
    673791  NM_207677.31  NP_997560.31 
     AA4375505  AF4575765  (see all 18)
    zebrafish
    (Danio rerio)
    dedd12   -- death effector domain-conatining 1 72.9(n) 58125  AF232226.1 
    About this table        Species with no ortholog for DEDD2

    ENSEMBL Gene Tree for DEDD2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for DEDD2 gene
    DEDD2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/60 NCBI SNPs in DEDD2 are shown (see all 60 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 27)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs37452291,2
    F,H47395008(+) GTGCTG/AGGAAG 1 -- ut31 ese35Minor allele frequency- A:0.02EA EU WA 1918
    rs72571701,2
    H47412975(+) CGGGGC/GCGGGG 1 A/P mis1 ese34Minor allele frequency- G:0.00EU EA WA 418
    rs10442321,2
    H47394683(-) ACCTTC/TTTGGA 1 -- ut315Minor allele frequency- T:0.01MN EU EA WA 602
    rs283647931,2
    F47413908(-) GAACCG/AGGTAC 1 -- ng312Minor allele frequency- A:0.06MN EA 1252
    rs10442311,2
    F47394688(-) CAGCCA/CCCTTC 1 -- ut311Minor allele frequency- C:0.01MN 184
    rs23036521,2
    C,F,H47395673(+) ACCACA/GGAGCC 1 S/S syn15Minor allele frequency- G:0.10EU EA WA NA 482
    rs81102411,2
    A,F,H47396936(+) TTAGGC/GTAGTG 1 -- int14Minor allele frequency- G:0.01EU EA WA 418
    rs37452311,2
    C,F,H47405675(+) GCCAAA/GGCCTA 1 -- int111Minor allele frequency- G:0.09NA EU EA WA 978
    rs81824911,2
    C,F,H47403864(+) TCAGCC/TCTGAC 1 -- int14Minor allele frequency- T:0.04EU EA WA 402
    rs169756841,2
    C,F,H47398150(+) TTACAG/ACCCTA 1 -- int115Minor allele frequency- A:0.05NA EU EA WA 1396
    About this table

    HapMap Linkage Disequilibrium images for DEDD2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    7 PubMed articles for DEDD2 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 162989 HGNC: 24450 AceView: DEDD2 Ensembl:ENSG00000160570 euGenes: HUgn162989
    ECgene: DEDD2 H-InvDB: DEDD2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DEDD2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DEDD2-related products

     Millipore Custom Antibody & Bulk Services
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     Millipore Immunoassay Services
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     Predesigned and custom siRNAs for DEDD2 Antibodies for DEDD2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for DEDD2 Browse small molecules at Sigma-Aldrich
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