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Aliases for DDX25 Gene

Aliases for DDX25 Gene

  • DEAD (Asp-Glu-Ala-Asp) Box Helicase 25 2 3
  • Gonadotropin-Regulated Testicular RNA Helicase 2 3 4
  • GRTH 3 4 6
  • DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 25 2 3
  • DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 25 2 3
  • DEAD Box Protein 25 3 4
  • ATP-Dependent RNA Helicase DDX25 3
  • EC 4
  • EC 3.6.1 63

External Ids for DDX25 Gene

Previous GeneCards Identifiers for DDX25 Gene

  • GC11P127774
  • GC11P127287
  • GC11P125806
  • GC11P125312
  • GC11P125279
  • GC11P121716
  • GC11P125773

Summaries for DDX25 Gene

Entrez Gene Summary for DDX25 Gene

  • DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. [provided by RefSeq, Jul 2008]

GeneCards Summary for DDX25 Gene

DDX25 (DEAD (Asp-Glu-Ala-Asp) Box Helicase 25) is a Protein Coding gene. GO annotations related to this gene include RNA binding and ATP-dependent RNA helicase activity. An important paralog of this gene is ENSG00000260537.

UniProtKB/Swiss-Prot for DDX25 Gene

  • ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DDX25 Gene

Genomics for DDX25 Gene

Regulatory Elements for DDX25 Gene

Genomic Location for DDX25 Gene

125,903,376 bp from pter
125,923,263 bp from pter
19,888 bases
Plus strand

Genomic View for DDX25 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for DDX25 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DDX25 Gene

Proteins for DDX25 Gene

  • Protein details for DDX25 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    ATP-dependent RNA helicase DDX25
    Protein Accession:
    Secondary Accessions:
    • B2R6Z0
    • Q5XVN2
    • Q86W81
    • Q8IYP1

    Protein attributes for DDX25 Gene

    483 amino acids
    Molecular mass:
    54692 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for DDX25 Gene

    Alternative splice isoforms for DDX25 Gene


neXtProt entry for DDX25 Gene

Proteomics data for DDX25 Gene at MOPED

Post-translational modifications for DDX25 Gene

  • Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus (By similarity).
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for DDX25 Gene

Domains for DDX25 Gene

Gene Families for DDX25 Gene

  • DDX :DEAD-boxes

Suggested Antigen Peptide Sequences for DDX25 Gene

Graphical View of Domain Structure for InterPro Entry



  • Q9UHL0
  • Contains 1 helicase ATP-binding domain.
  • Contains 1 helicase C-terminal domain.
  • Belongs to the DEAD box helicase family.
genes like me logo Genes that share domains with DDX25: view

Function for DDX25 Gene

Molecular function for DDX25 Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + H(2)O = ADP + phosphate
UniProtKB/Swiss-Prot Function: ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).
UniProtKB/Swiss-Prot Induction: Up-regulated at transcriptional level by chorionic gonadotropin via cyclic AMP-induced androgen formation in the Leydig cell.

Enzyme Numbers (IUBMB) for DDX25 Gene

Gene Ontology (GO) - Molecular Function for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding --
GO:0003723 RNA binding IEA --
GO:0004004 ATP-dependent RNA helicase activity ISS --
GO:0004386 helicase activity --
GO:0005524 ATP binding ISS --
genes like me logo Genes that share ontologies with DDX25: view
genes like me logo Genes that share phenotypes with DDX25: view

Animal Models for DDX25 Gene

MGI Knock Outs for DDX25:

Animal Model Products

CRISPR Products

miRNA for DDX25 Gene

miRTarBase miRNAs that target DDX25

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for DDX25

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for DDX25 Gene

Localization for DDX25 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DDX25 Gene

Cytoplasm. Nucleus. Note=Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids (By similarity). {ECO:0000250}.

Subcellular locations from

Jensen Localization Image for DDX25 Gene COMPARTMENTS Subcellular localization image for DDX25 gene
Compartment Confidence
nucleus 4
cytosol 3
cytoskeleton 1

Gene Ontology (GO) - Cellular Components for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus ISS --
GO:0005730 nucleolus IBA --
GO:0005737 cytoplasm ISS --
GO:0033391 chromatoid body ISS --
genes like me logo Genes that share ontologies with DDX25: view

Pathways for DDX25 Gene

SuperPathways for DDX25 Gene

No Data Available

Interacting Proteins for DDX25 Gene

Gene Ontology (GO) - Biological Process for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006200 obsolete ATP catabolic process --
GO:0006406 mRNA export from nucleus ISS --
GO:0006417 regulation of translation ISS --
GO:0007275 multicellular organismal development IEA --
GO:0007286 spermatid development ISS --
genes like me logo Genes that share ontologies with DDX25: view

No data available for Pathways by source for DDX25 Gene

Compounds for DDX25 Gene

(1) Novoseek inferred chemical compound relationships for DDX25 Gene

Compound -log(P) Hits PubMed IDs
testosterone 44.5 11
genes like me logo Genes that share compounds with DDX25: view

Transcripts for DDX25 Gene

Unigene Clusters for DDX25 Gene

DEAD (Asp-Glu-Ala-Asp) box helicase 25:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for DDX25

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for DDX25 Gene

No ASD Table

Relevant External Links for DDX25 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for DDX25 Gene

mRNA expression in normal human tissues for DDX25 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for DDX25 Gene

This gene is overexpressed in Testis (9.9), Brain - Cortex (4.1), and Brain - Frontal Cortex (BA9) (4.0).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for DDX25 Gene

SOURCE GeneReport for Unigene cluster for DDX25 Gene Hs.420263

mRNA Expression by UniProt/SwissProt for DDX25 Gene

Tissue specificity: Highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus.
genes like me logo Genes that share expressions with DDX25: view

In Situ Assay Products

Orthologs for DDX25 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for DDX25 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia DDX25 35
  • 99.72 (n)
  • 99.59 (a)
DDX25 36
  • 100 (a)
(Bos Taurus)
Mammalia DDX25 35
  • 89.03 (n)
  • 92.55 (a)
DDX25 36
  • 92 (a)
(Canis familiaris)
Mammalia DDX25 35
  • 90.97 (n)
  • 94.58 (a)
DDX25 36
  • 93 (a)
(Mus musculus)
Mammalia Ddx25 35
  • 87.65 (n)
  • 93.17 (a)
Ddx25 16
Ddx25 36
  • 93 (a)
(Monodelphis domestica)
Mammalia DDX25 36
  • 90 (a)
(Ornithorhynchus anatinus)
Mammalia DDX25 36
  • 81 (a)
(Rattus norvegicus)
Mammalia Ddx25 35
  • 87.51 (n)
  • 93.58 (a)
(Gallus gallus)
Aves DDX25 35
  • 68.16 (n)
  • 68.79 (a)
DDX25 36
  • 67 (a)
(Anolis carolinensis)
Reptilia DDX25 36
  • 64 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia ddx25 35
  • 66.29 (n)
  • 68.56 (a)
(Danio rerio)
Actinopterygii zgc:193690 36
  • 60 (a)
fruit fly
(Drosophila melanogaster)
Insecta Dbp80 36
  • 51 (a)
Dbp80 37
  • 58 (a)
(Caenorhabditis elegans)
Secernentea ddx-19 36
  • 21 (a)
T07D4.4c 37
  • 48 (a)
T07D4.4a 37
  • 48 (a)
T07D4.4b 37
  • 48 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes DBP5 36
  • 41 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8406 36
  • 55 (a)
Species with no ortholog for DDX25:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DDX25 Gene

Gene Tree for DDX25 (if available)
Gene Tree for DDX25 (if available)

Paralogs for DDX25 Gene

Paralogs for DDX25 Gene Pseudogenes for DDX25 Gene

genes like me logo Genes that share paralogs with DDX25: view

Variants for DDX25 Gene

Sequence variations from dbSNP and Humsavar for DDX25 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs472535 -- 125,914,951(-) aaact(A/G)gccaa intron-variant
rs478545 -- 125,915,904(-) AGTTA(C/T)CTAAA intron-variant
rs485878 -- 125,907,161(+) AGAAC(A/G)AGAAA intron-variant
rs504251 -- 125,910,807(+) TGGGG(A/G)AAAAA intron-variant
rs505183 -- 125,910,909(+) TTTGT(G/T)TTTTG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DDX25 Gene

Variant ID Type Subtype PubMed ID
esv32964 CNV Gain+Loss 17666407
dgv248n67 CNV Gain 20364138
nsv535 CNV Loss 18451855

Relevant External Links for DDX25 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DDX25 Gene

Disorders for DDX25 Gene

(1) University of Copenhagen DISEASES for DDX25 Gene

Relevant External Links for DDX25

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with DDX25: view

No data available for UniProtKB/Swiss-Prot for DDX25 Gene

Publications for DDX25 Gene

  1. A novel gonadotropin-regulated testicular RNA helicase: a new member of the DEAD-box family. (PMID: 10608860) Tang P.-Z. … Dufau M.L. (J. Biol. Chem. 1999) 2 3 4 23
  2. Single nucleotide polymorphisms of the gonadotrophin-regulated testicular helicase (GRTH) gene may be associated with the human spermatogenesis impairment. (PMID: 16293649) A Z. … Zhang W. (Hum. Reprod. 2006) 3 23 48
  3. Polymorphism of the GRTH/DDX25 gene in normal and infertile Japanese men: a missense mutation associated with loss of GRTH phosphorylation. (PMID: 17848414) Tsai-Morris C.H. … Dufau M.L. (Mol. Hum. Reprod. 2007) 3 23 48
  4. Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25): a multifunctional protein essential for spermatogenesis. (PMID: 19875492) Tsai-Morris C.H. … Dufau M.L. (J. Androl. 2010) 3 23
  5. Explorative study to identify novel candidate genes related to oxaliplatin efficacy and toxicity using a DNA repair array. (PMID: 19536092) Kweekel D.M. … Guchelaar H.J. (Br. J. Cancer 2009) 3 48

Products for DDX25 Gene

Sources for DDX25 Gene

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