V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

DDX10 Gene

protein-coding   GIFtS: 63

GC11P108041
DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
(Previous name: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 10 (RNA helicase) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 3.6.1.- 3
HRH-J8 1, 2
Descriptions
DDX10-NUP98 fusion protein type 2 2
DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 2
DEAD box protein 10 3
DEAD box-10 2
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 10 (RNA
helicase) 1, 2
External Ids
HGNC: 27351
Entrez Gene: 16622
UniProtKB: Q132063
Ensembl: ENSG000001781057
Search outside databases for aliases for DDX10 gene

Previous GC identifers: GC11P110792 GC11P109988 GC11P108569 GC11P108073

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for DDX10:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA
helicases. They are implicated in a number of cellular processes involving alteration of RNA
secondary structure such as translation initiation, nuclear and mitochondrial splicing, and
ribosome and spliceosome assembly. Based on their distribution patterns, some members of this
family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and
division. This gene encodes a DEAD box protein, and it may be involved in ribosome assembly.
Fusion of this gene and the nucleoporin gene, NUP98, by inversion 11 (p15q22) chromosome
translocation is found in the patients with de novo or therapy-related myeloid malignancies.
[provided by RefSeq]

UniProtKB/Swiss-Prot: DDX10_HUMAN, Q13206
Function: Putative ATP-dependent RNA helicase

Gene Wiki entry for DDX10

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DDX10 gene  

Entrez Gene cytogenetic band: 11q22-q23   Ensembl cytogenetic band:  11q22.3   HGNC cytogenetic band: 11q22-q23

DDX10 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P108041:     (about GC identifiers)

Start:
108,041,014 bp from pter
End:
108,316,866 bp from pter
Size:
275,853 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000011.8  NT_033899.7  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: DDX10_HUMAN, Q13206 (See protein sequence)
Recommended Name: Probable ATP-dependent RNA helicase DDX10  
Size: 875 amino acids; 100888 Da
PDB structures from and Proteopedia :
2PL3 (3D)    
Secondary accessions: Q5BJD8

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_004389.2  

    ENSEMBL proteins: 
    ENSP00000314348 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (DDX10)
    Human Recombinant Proteins from Abnova (DDX10)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005730 nucleolus IDA18029348
    GO:0005886 plasma membrane IDA18029348
    About this table

    Antibodies for DDX10: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for DDX10
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (DDX10), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Novus Biologicals Antibodies for DDX10

    Assays for DDX10: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/6 InterPro domains/families (see all 6 ):
     IPR011545 DNA/RNA_helicase_DEAD/DEAH_N
     IPR000629 RNA-helicase_DEAD-box_CS
     IPR014014 RNA_helicase_DEAD_Q_motif
     IPR014001 DEAD-like_N
     IPR001650 DNA/RNA_helicase_C


       GeneDecks  DDX10 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q13206

    ProtoNet protein and cluster: Q13206

    1 Blocks protein family: IPB000629 ATP-dependent helicase

    UniProtKB/Swiss-Prot: DDX10_HUMAN, Q13206
    Domain: The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and
    controls ATP binding and hydrolysis
    Similarity: Belongs to the DEAD box helicase family. DDX10/DBP4 subfamily
    Similarity: Contains 1 helicase ATP-binding domain
    Similarity: Contains 1 helicase C-terminal domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DDX10)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DDX10)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004398

                  Applied Biosystems Silencer® siRNAs for DDX10

                  Sigma-Aldrich siRNA for DDX10  
                         Browse shRNA at Sigma-Aldrich  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for DDX10
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004398
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004398
                                     untagged cDNA clone in CMV expression vector: NM_004398 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_004398

    UniProtKB/Swiss-Prot: DDX10_HUMAN, Q13206
    Function: Putative ATP-dependent RNA helicase
    Enzyme Number (IUBMB): EC 3.6.1.- 

    Genatlas biochemistry entry for DDX10:
    DEAD/H box protein 10,ubiquitously expressed,fused with NUP98 in de novo or therapy related myeloid
    maligancies with inv 11(p15-q22)
    1 MGI phenotypic allele for Ddx10 (no phenotypes)

    5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003723 RNA binding IEA--
    GO:0003724 RNA helicase activity TAS8660968
    GO:0005524 ATP binding IEA--
    GO:0008026 ATP-dependent helicase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/14 Interacting proteins for DDX10 (ENSP000003143483) via UniProtKB, MINT, and/or STRING (see all 14 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    NUP98ENSP000003160323STRING (score=.92)
    RAP1GDS1ENSP000003404543STRING (score=.901)
    WHSC1L1ENSP000003139833STRING (score=.874)
    PRRX1ENSP000003567343STRING (score=.866)
    HOXC13ENSP000002430563STRING (score=.861)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DDX10

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DDX10)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DDX10)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_004398

                  Sigma-Aldrich siRNA for DDX10  
                         Browse shRNA at Sigma-Aldrich  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004398  

    REFSEQ mRNAs for DDX10 gene: 

    NM_004398.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004398  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_004398
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_004398
                                     untagged cDNA clone in CMV expression vector: NM_004398 

    Additional cDNA sequence: 

    AB000267.1 AB001343.1 AB040537.1 AB209884.1 AK296752.1 AK315216.1 AL833534.1 BC049217.1 
    BC064921.1 BC091521.1 BC093654.1 BC093656.1 U28042.1 

    5 DOTS entries:

    DT.310453  DT.97799397  DT.100782442  DT.100736227  DT.100782435 

    24/105 AceView cDNA sequences (see all 105 ):

    BU159524 AA373519 BU539873 BM561644 NM_004398 BX283454 AB040537 BQ941659 
    BU170172 BC049217 BX489177 CA429323 BU159235 AA809921 BC064921 BU619096 
    BG821727 AA084582 BF934536 AL043665 CD654730 BU616591 CB163252 BX283469 

    highest scoring ESTs for DDX10:

    U28042 AA604373 AA629601 AB040537 AI376353 AI689145 AI758573 AI766144 AL043665 AL043712 

    Unigene Cluster for DDX10:

    DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
    Hs.591931  [show with all ESTs]
    Unigene Representative Sequence: AB209884


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000322536  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DDX10 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DDX10

    1 / 2 / 3

    3 probe-sets matching DDX10 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    831_at2, 3 U95-A 2 1.00 0.94 1.00 1.00 U28042 1.00 1.00 1.00 1

    204977_at2, 3 U133-A 2 1.00 0.95 -- -- NM_004398 0.60 1.00 0.82 1

    204977_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ATTTCGACGT

    SOURCE GeneReport for Unigene cluster: Hs.591931

    Expression variation in blood from EXPOLDB for DDX10

    UniProtKB/Swiss-Prot: DDX10_HUMAN, Q13206
    Tissue specificity: High in testis but widely expressed

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DDX10 gene from 5/19 species (see all 19 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DDX101   -- DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 89.76(n)
    89.95(a)
    479448  XM_536583.2  XP_536583.2 
    chimpanzee
    (Pan troglodytes)
    DDX101   -- DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 99.43(n)
    99.09(a)
    451534  XM_001141618.1  XP_001141618.1 
    cow
    (Bos taurus)
    DDX101   -- DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 89.78(n)
    90.01(a)
    528483  NM_001076881.1  NP_001070349.1 
    rat
    (Rattus norvegicus)
    Ddx101   -- DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 86.32(n)
    86.55(a)
    300710  XM_236263.4  XP_236263.4 
    mouse
    (Mus musculus)
    Ddx101, 5 95
    DEAD (Asp-Glu-Ala-Asp) box polypeptide 101, 5 86.41(n)1
    85.53(a)1
    775911  XM_284494.51  XP_284494.31 
     AI6460545  AK0194955  (see all 16)
    About this table        Species with no ortholog for DDX10

    ENSEMBL Gene Tree for DDX10
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for DDX10 gene
    DDX182  DDX312  DDX552  

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/1199 NCBI SNPs in DDX10 are shown (see all 1199 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 405)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 11 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs23061181,2
    A,C,F,H,O108041002(-) GCACAG/AAGGCG 1 -- ng514Minor allele frequency- A:0.40EU EA WA 414
    rs38584001,2
    A,C,F,H108039770(+) AGTGTT/CCCTGA 1 -- ng516Minor allele frequency- C:0.11EU EA WA NA 596
    --
    rs71039441,2
    F,H108316690(+) GTGTCA/GTTGTT 1 -- ut314Minor allele frequency- G:0.01EU EA WA 420
    rs38090321,2
    F,H108040926(+) GCCCTC/ACTTTT 1 -- ng51 trp34Minor allele frequency- A:0.02EU EA WA 416
    rs171087811,2
    F,H108316786(+) AGACTA/GTGATG 1 -- ut31 ese37Minor allele frequency- G:0.05NA EU EA WA 562
    --
    rs171087851,2
    F108317219(+) CAAATA/GACTCA 1 -- ng313Minor allele frequency- G:0.01NA 142
    rs47543421,2
    A108040834(+) CCGGCG/TCGGCT 1 -- ng510--------
    --
    rs37589091,2
    C108040180(-) gaatcA/Gcttga 1 -- ng510--------
    --
    rs22286491,2
    H108091827(+) TCCAGA/GAAAAC 1 E/G mis10--------
    --
    rs38583991,2
    A108039650(+) GCTATA/GCATAC 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for DDX10 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601235

    UniProtKB/Swiss-Prot: DDX10_HUMAN, Q13206
  • Defects in DDX10 may be a cause of breast cancer
  • 1 Novoseek disease relationship for DDX10 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    myelodysplastic syndromes 52.91 1 10830185 (1)
    About this table

    1 PharmGKB disease relationship for DDX10 gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/21 PubMed articles for DDX10 gene (see all 21 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 1662 HGNC: 2735 AceView: DDX10 Ensembl:ENSG00000178105 euGenes: HUgn1662
    ECgene: DDX10 H-InvDB: DDX10
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for DDX10 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DDX10:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DDX10-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for DDX10 Antibodies for DDX10
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Browse shRNAs at Sigma-Aldrich Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

     Recombinant Proteins (DDX10)
     Search for Antibodies
     Chimera RNAi (DDX10)
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Recombinant Proteins
    (DDX10)
     Antibodies (DDX10)
     Search OriGene for DDX10
     Search Tocris compounds for DDX10




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for DDX10

    GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

    Random Gene From GIFtS : about GIFtS

    Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

    Developed at the Crown Human Genome Center & Weizmann Institute of Science



    The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
    DDX10 Gene at Home site.
    Version 2.41.1
    server5.xennexinc.com