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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

DDIT3 Gene

protein-coding   GIFtS: 66
GCID: GC12M057910

DNA-damage-inducible transcript 3
 Explore 70 diseases affiliated with
DDIT3 via
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 Human Malady Compendium 
Biological research products
for DDIT3    

Aliases
for DDIT3 gene

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section
Aliases
DNA-Damage-Inducible Transcript 31 2     DDIT-32 3
CHOP101 2 3 5     C/EBP Zeta2 3
GADD1531 2 3 5     CCAAT/Enhancer-Binding Protein Homologous Protein2 3
CHOP1 2 3     CEBPZ2
C/EBP-Homologous Protein 102 3     DNA Damage-Inducible Transcript 3 Protein2
Growth Arrest And DNA Damage-Inducible Protein GADD1532 3     C/EBP-Homologous Protein3
CHOP-102 3     

External Ids:    HGNC: 27261   Entrez Gene: 16492   Ensembl: ENSG000001751977   OMIM: 1263375   UniProtKB: P356383   

Export aliases for DDIT3 gene to outside databases

Previous GC identifers: GC12M059181 GC12M058053 GC12M057627 GC12M056196 GC12M054948


Summaries
for DDIT3 gene

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section
Entrez Gene summary for DDIT3:
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein
functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP
(liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and
erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on
chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or
Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have
been identified. (provided by RefSeq, Aug 2010)

UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638
Function: Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide
variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both
as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a
dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their
association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes.
Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA,
BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation
of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated
expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway
by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a
regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the
activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is
involved in the inflammatory response

Gene Wiki entry for DDIT3 (DNA damage-inducible transcript 3)


Genomic Views
for DDIT3 gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_029419.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the DDIT3 gene promoter:
         Max1   PPAR-gamma1   PPAR-gamma2   c-Myc   C/EBPalpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: DDIT3 promoter sequence
   Search SABiosciences Chromatin IP Primers for DDIT3

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DDIT3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.1-q13.2   Ensembl cytogenetic band:  12q13.3   HGNC cytogenetic band: 12q13.1-q13.2

DDIT3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DDIT3 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M057910:  view genomic region     (about GC identifiers)

Start:
 57,910,371 bp from pter      End:
 57,914,300 bp from pter
Size:
 3,930 bases      Orientation:
 minus strand

Proteins
for DDIT3 gene

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section
UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638 (See protein sequence)
Recommended Name: DNA damage-inducible transcript 3 protein  
Size: 169 amino acids; 19175 Da
Subunit: Heterodimer. Interacts with TCF7L2/TCF4, EP300/P300, HDAC1, HDAC5 and HDAC6. Interacts with TRIB3 which blocks
its association with EP300/P300. Interacts with FOXO3, CEBPB and ATF4
Subcellular location: Cytoplasm. Nucleus. Note=Present in the cytoplasm under non-stressed conditions and ER stress
leads to its nuclear accumulation
Sequence caution: Sequence=AAB27103.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown
origin in the N-terminal part;

Explore the universe of human proteins at neXtProt for DDIT3: NX_P35638

Post-translational modifications:

  • Ubiquitinated, leading to its degradation by the proteasome1
  • Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at
    • serine residues by CK2 inhibits its transcriptional activation activity (By similarity)1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P35638

    • DDIT3 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (6 alternative transcripts): 
    NP_001181982.1  NP_001181983.1  NP_001181984.1  NP_001181985.1  NP_001181986.1  NP_004074.2  

    ENSEMBL proteins: 
     ENSP00000447188   ENSP00000448665   ENSP00000340671   ENSP00000447803   ENSP00000447503  
    Reactome Protein details: P35638
    Human Recombinant Protein Products: 
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    Novus Biologicals DDIT3 Proteins
    Novus Biologicals DDIT3 Lysate
    Browse Sino Biological Recombinant Proteins
    ProSpec Recombinant Protein for DDIT3
    Uscn Proteins for DDIT3

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005737cytoplasm IEA--


    DDIT3 for ontologies           About GeneDecksing



    DDIT3 Antibody Products: 
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    Novus Biologicals DDIT3 Antibodies
    Search for Antibodies for DDIT3 at Abcam  
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    ThermoFisher Antibody for DDIT3

    Assay Products for DDIT3: 
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    Uscn ELISAs and CLIAs for DDIT3

    Protein Domains /
    Families
    for DDIT3 gene

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    DDIT3 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR016670 DNA_damage_induc_transcript_3
     IPR004827 bZIP

    Graphical View of Domain Structure for InterPro Entry P35638

    ProtoNet protein and cluster: P35638

    UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638
    Domain: The N-terminal region is necessary for its proteasomal degradation, transcriptional activity and interaction
    with EP300/P300
    Similarity: Belongs to the bZIP family
    Similarity: Contains 1 bZIP (basic-leucine zipper) domain


    Function
    for DDIT3 gene

    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section
    Function Summary:

         UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638
    Function: Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide
    variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both
    as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a
    dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their
    association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes.
    Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA,
    BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation
    of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated
    expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway
    by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a
    regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the
    activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is
    involved in the inflammatory response
    Induction: By oxidative stress, amino-acid deprivation, hypoxia and ER stress. During ER stress, induced by a
    EIF2AK3/ATF4 pathway and/or ERN1/ATF6 pathway. Expression is suppressed by TLR-TRIF signaling pathway during prolonged
    ER stress

         Genatlas biochemistry entry for DDIT3:
    DDIT3 dominant negative regulator of several C/EBP transcription factors,involved in t(12;16) translocation (see
    FUS),involved in erythroid growth and differentiation possibly through interaction with both C/EBP and non C/EBP
    family members

    10/384 SABiosciences Target genes for DDIT3 (see all 384):
    AARS2 ABCA10 ACSF3 ACYP2 ADAL ADAM21 ADAM33 ADAMTS18 ADC ALKBH1

    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat DDIT3
    4 QIAGEN miScript miRNA Assays for microRNAs that regulate DDIT3:
    hsa-miR-4314 hsa-miR-1271 hsa-miR-96 hsa-miR-3153
    SwitchGear 3'UTR luciferase reporter plasmid: DDIT3 3' UTR sequence
    Inhib. RNA
    Products:            		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for DDIT3 (see all 7)
    OriGene shRNA RFP: DDIT3
    OriGene siRNA: DDIT3
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat DDIT3

    Gene Editing
    Products:    		DNA2.0 Custom Protein Engineering Service for DDIT3

    Clone
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    OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DDIT3 (see all 4)
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    GenScript: all cDNA clones in your preferred vector (see all 6): DDIT3 (NM_001195053)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DDIT3 

    Cell Line
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    Search LifeMap BioReagents cell lines for DDIT3

    In Situ Assay
    Products:       
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    Gene Ontology (GO): 5/7 molecular function terms (GO ID links to tree view) (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IDA14667815
    GO:0003700sequence-specific DNA binding transcription factor activity IEA--
    GO:0003714transcription corepressor activity TAS7503811
    GO:0005515protein binding IPI--
    GO:0008134transcription factor binding IPI16434966


    DDIT3 for ontologies           About GeneDecksing


    3 GenomeRNAi human phenotypes for DDIT3:
     Decreased NANOG protein expres  Decreased OCT4 protein express  Decreased POU5F1-GFP protein e 

    Animal Models:
         Mouse knock-outs for DDIT3: Ddit3tm1Mori Ddit3tm1Dron
         6 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Ddit3):
     behavior/neurological  cellular  endocrine/exocrine gland  homeostasis/metabolism  immune system 
     nervous system 

    DDIT3 for phenotypes           About GeneDecksing


    Pathways & Interactions
    for DDIT3 gene

    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/17 super-pathways (see all 17) About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Activation of Chaperone Genes by ATF6-alpha
    		Activation of Chaperone Genes by ATF6-alpha1.00
    		Activation of Chaperones by ATF6-alpha0.62
    2Activation of Genes by ATF4
    		Activation of Genes by ATF41.00
    		PERK regulated gene expression0.64
    3MAPK signaling pathway
    		MAPK signaling pathway1.00
    		MAPK signaling pathway0.50
    4Development_CNTF receptor signaling
    		Development_Growth hormone signaling via PI3K/AKT and MAPK cascades0.34
    		Development Growth hormone signaling via PI3K/AKT and MAPK cascades0.34
    5Expression of CHOP (DDIT3, GADD153)
    		Expression of CHOP (DDIT3, GADD153)1.00

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    1 EMD Millipore Pathway for DDIT3
        Development Growth hormone signaling via PI3K/AKT and MAPK cascades

    2 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for DDIT3
        MAPK Signaling
    p38 Signaling

    1 Cell Signaling Technology (CST) Pathway for DDIT3
        Translational Control

    1 GeneGo (Thomson Reuters) Pathway for DDIT3
        Development Growth hormone signaling via PI3K/AKT and MAPK cascades

    5/6 BioSystems Pathways for DDIT3 (see all 6) 
        Adipogenesis
    MAPK signaling pathway
    p38 MAPK Signaling Pathway
    ATF-2 transcription factor network
    Validated targets of C-MYC transcriptional repression

    5/7        Reactome Pathways for DDIT3 (see all 7)
        Activation of Chaperones by ATF6-alpha
    PERK regulated gene expression
    Expression of CHOP (DDIT3, GADD153)
    Metabolism of proteins
    Activation of Chaperone Genes by ATF6-alpha

    1 PharmGKB Pathway for DDIT3
        Celecoxib Pathway, Pharmacodynamics

    2         Kegg Pathways  (Kegg details for DDIT3):
        MAPK signaling pathway
    Protein processing in endoplasmic reticulum


    DDIT3 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for DDIT3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/391 Interacting proteins for DDIT3 (P356382, 3 ENSP000003406714) via UniProtKB, MINT, STRING, and/or I2D (see all 391)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): 5/33 biological process terms (GO ID links to tree view) (see all 33):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001975response to amphetamine ----
    GO:0006351transcription, DNA-dependent ----
    GO:0006355regulation of transcription, DNA-dependent IMP17872950
    GO:0006974response to DNA damage stimulus TAS1339368
    GO:0006979response to oxidative stress ----


    DDIT3 for ontologies           About GeneDecksing



    Drugs & Compounds
    for DDIT3 gene

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    DDIT3 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for DDIT3
    10/64 Novoseek chemical compound relationships for DDIT3 gene (see all 64)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tunicamycin 80.2 26 15775988 (3), 18228003 (2), 17825316 (1), 15605392 (1) (see all 10)
    n-(4-hydroxyphenyl)retinamide 75.8 58 12353227 (8), 12234979 (6), 12138118 (5), 15917187 (5) (see all 10)
    l-(4,5-3h)leucine 71.7 1 11113529 (1)
    thapsigargin 65.6 22 15322075 (3), 9365549 (2), 11389688 (1), 18266951 (1) (see all 11)
    pga2 62.7 12 1735464 (6), 7926370 (1)
    methylmethanesulfonate 59.8 7 8892753 (2), 8754828 (1), 9586958 (1), 1400365 (1)
    n acetylcysteine 54.2 21 19722195 (2), 8670069 (1), 15271854 (1), 9044846 (1) (see all 12)
    sp 600125 53.4 6 15466475 (1), 15680548 (1), 18767967 (1), 19139269 (1)
    indole-3-carbinol 45.4 1 15681163 (1)
    deferoxamine mesylate 44.1 2 14604972 (1)

    Search CenterWatch for drugs/clinical trials and news about DDIT3 

    Transcripts
    for DDIT3 gene

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section
    REFSEQ mRNAs for DDIT3 gene (6 alternative transcripts): 
    NM_001195053.1  NM_001195054.1  NM_001195055.1  NM_001195056.1  NM_001195057.1  NM_004083.5  

    Unigene Cluster for DDIT3:

    DNA-damage-inducible transcript 3
    Hs.505777  [show with all ESTs]
    Unigene Representative Sequence: NM_001195056
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000547303 ENST00000551116(uc001soi.3 uc009zpt.3 uc021qzj.1 uc021qzk.1 uc021qzl.1 uc009zps.3)
    ENST00000550148 ENST00000346473 ENST00000552740 ENST00000547526

    miRNA
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    SwitchGear 3'UTR luciferase reporter plasmid: DDIT3 3' UTR sequence
    Inhib. RNA
    Products:             		Browse for Gene Knock-down Tools from EMD Millipore
    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for DDIT3 (see all 7)
    OriGene shRNA RFP: DDIT3
    OriGene siRNA: DDIT3
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat DDIT3
    Clone
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    OriGene custom cloning services – gene synthesis, subcloning, mutagenesis, variant library, vector shuttling 
    GenScript: all cDNA clones in your preferred vector (see all 6): DDIT3 (NM_001195053)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DDIT3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DDIT3 
    Primer
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DDIT3
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DDIT3

    Additional cDNA sequence: 

    AK316581.1 AY461580.1 BC003637.2 BT006691.1 S40706.1 

    12 DOTS entries:

    DT.91780645  DT.100045496  DT.97810576  DT.95193903  DT.100687083  DT.121187388  DT.91780612  DT.40306179 
    DT.40236699  DT.91780636  DT.100687093  DT.121187409 

    24/277 AceView cDNA sequences (see all 277):

    BU663391 BM759769 R51042 AW043601 BQ228244 BM693345 BQ019188 CA441283 
    CB144081 BQ224643 BM823128 AA812094 CD675012 BQ128379 CR616853 BM830819 
    BQ721406 CA425724 BF968597 BX509791 BM831039 AW302469 BQ694049 BM669449 

    GeneLoc Exon Structure

    5/8 Alternative Splicing Database (ASD) splice patterns (SP) for DDIT3 (see all 8)    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b · 3c · 3d · 3e · 3f ^ 4a · 4b · 4c · 4d · 4e
    SP1:              -           -     -     -     -                                             
    SP2:              -           -     -     -                                                   
    SP3:              -                                                                           
    SP4:              -           -                                                               
    SP5:              -           -     -     -     -     -                                       


    ECgene alternative splicing isoforms for DDIT3

    Expression
    for DDIT3 gene

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    DDIT3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: AACAATTGGG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)
    About this image

    DDIT3 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    LimbLimb BudLimb Bud Mesenchyme CellsMesoderm
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    N2/LSB/S/F8/CHIR-induced cells (Generation of midbra...)

    See DDIT3 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for DDIT3

    SOURCE GeneReport for Unigene cluster: Hs.505777
        SABiosciences Expression via Pathway-Focused PCR Arrays including DDIT3 (see all 11): 
              DNA Damage Signaling Pathway in human mouse rat
              Cancer PathwayFinder in human mouse rat
              Stress & Toxicity PathwayFinder in human mouse rat
              Adipogenesis in human mouse rat
              cAMP / Ca2+ Signaling PathwayFinder in human mouse rat

    Primer
    Products:    		 OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DDIT3
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    In Situ
    Assay Products:     
         		Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DDIT3

    Orthologs
    for DDIT3 gene

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section
    This gene was present in the common ancestor of chordates.

    Orthologs for DDIT3 gene from 3/7 species (see all 7)    About this table
    Organism Taxonomic
    classification    		 Gene Description Human
    Similarity    		 Orthology
    Type    		 Details
    mouse
    (Mus musculus)    		 Mammalia Ddit31 , 5 DNA-damage inducible transcript 31, 5 87.7(n)1
    88.69(a)1    		   10 (74.50 cM)5
    131981  NM_007837.31  NP_031863.31 
     1272907745 
    lizard
    (Anolis carolinensis)    		 Reptilia DDIT36
    		 --
    		 47(a)
    		 1 ↔ 1
    		 GL343212.1(576541-579441)
    zebrafish
    (Danio rerio)    		 Actinopterygii ddit36
    		 DNA-damage-inducible transcript 3
    		 32(a)
    		 1 ↔ 1
    		 6(58818530-58821727)


    ENSEMBL Gene Tree for DDIT3 (if available)
    TreeFam Gene Tree for DDIT3 (if available) 

    Paralogs
    for DDIT3 gene

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    		  --

    Genomic Variants
    for DDIT3 gene

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/96 NCBI SNPs in DDIT3 are shown (see all 96)     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance    		Chr 12 posSequence#AA
    Chg    		TypeMore#Allele
    freq    		PopTotal
    sample    		More
    ----------
    rs45830181,2
    		C--54948211(+) TTTATC/TTTAGG 1 -- int11Minor allele frequency- T:0.00NA 2
    rs115470201,2
    		C--54948546(-) CCCACC/TTTTCC 1 -- int12Minor allele frequency- T:0.00NA 4
    rs22911361,2
    		C--54948969(-) CATTTC/ACTTGA 1 -- int12Minor allele frequency- A:0.00NA 4
    rs283828511,2
    		C,F,H,--54949307(-) CTACAA/GAAACA 1 -- int110Minor allele frequency- G:0.02NS NA WA 998
    rs118346541,2
    		C,H--54949601(+) TTTTTC/TTTTTT 1 -- int1 trp30--------
    rs283828491,2
    		C,F,--54949698(-) CCGAGG/AAGGCA 1 -- int15Minor allele frequency- A:0.02NS 188
    rs47602981,2
    		C,F,A,--54949727(+) CTCGGC/TCTCCC 1 -- int15Minor allele frequency- T:0.05NS 184
    rs283828461,2
    		C,F,--54949736(-) CAGCTA/GCTCGG 1 -- int15Minor allele frequency- G:0.01NS 190
    rs283828451,2
    		C,F,--54949921(-) AGGAGG/-CTGGA 1 -- int17Minor allele frequency- -:0.06NS NA 194
    rs283828431,2
    		C,F,H,--54950039(-) AGGACT/CACAAA 1 -- int112Minor allele frequency- C:0.05NS NA WA 1198

    HapMap Linkage Disequilibrium report for DDIT3 (57910371 - 57914300 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for DDIT3
         1 CNV: 3891

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DDIT3
    DNA2.0 Custom Variant and Variant Library Synthesis for DDIT3

    Disorders / Diseases
    for DDIT3 gene

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    DDIT3 for disorders           About GeneDecksing

    OMIM gene information: 126337   
    OMIM disorders: 613488  
    UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638
  • Note=A chromosomal aberration involving DDIT3 is found in a patient with malignant myxoid liposarcoma.
    • Translocation t(12;16)(q13;p11) with FUS

    20/70 diseases for DDIT3 (see all 70):    About MalaCards
    myxoid liposarcoma    liposarcoma    sarcoma    malignant fibrous histiocytoma
    fibrous histiocytoma    familial adenomatous polyposis    west nile virus    non-small cell lung carcinoma
    adenomatous polyposis coli    histiocytoma    bipolar i disorder    clear cell sarcoma
    lipoma    polyposis    pleomorphic adenoma    hepatitis e
    gestational diabetes    chronic lymphocytic leukemia    acute myeloid leukemia    lymphocytic leukemia

    1 disease from the University of Copenhagen DISEASES database for DDIT3:
    Liposarcoma

    10/36 Novoseek disease relationships for DDIT3 gene (see all 36)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    liposarcoma myxoid 91.3 26 18162764 (3), 8143276 (2), 8637704 (2), 8510758 (2) (see all 17)
    liposarcoma 79.1 29 11126370 (2), 12168660 (2), 9676855 (1), 12169678 (1) (see all 18)
    sarcoma 45.1 8 18162764 (2), 7687873 (2), 8117620 (1), 10448063 (1)
    cancer 39.9 22 15322075 (3), 16463383 (2), 12138118 (2), 19276161 (1) (see all 12)
    lipoma 34.2 2 9244875 (1), 20332486 (1)
    colon cancer 32.9 6 18534616 (1), 15271854 (1), 16434966 (1), 17180111 (1) (see all 6)
    ewings sarcoma 31.7 2 7493135 (1), 20332486 (1)
    tumors 31.6 31 10430059 (4), 10803921 (3), 10534579 (2), 8117620 (2) (see all 20)
    cell damage 25.6 2 11113529 (2)
    malignant fibrous histiocytoma 22.5 1 8143276 (1)

    Genatlas disease: DDIT3
    myxoid liposarcoma,with breakpoint in t(12;16) translocation (see FUS)

    Genetic Association Database (GAD): DDIT3
    Human Genome Epidemiology (HuGE) Navigator: DDIT3 (4 documents)

    Export disorders for DDIT3 gene to outside databases

    Publications
    for DDIT3 gene

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for DDIT3 gene, integrated from 9 sources (see all 409):
    (articles sorted by number of sources associating them with DDIT3)    		    Utopia: connect your pdf to the dynamic
    world of online information

    1. Critical and functional regulation of CHOP (C/EBP homologous protein) through the N-terminal portion. (PubMed id 17872950)1, 2, 9 Ohoka N....Hayashi H. (2007)
    2. The C/EBP homologous protein CHOP (GADD153) is an inhibitor of Wnt/TCF signals. (PubMed id 16434966)1, 2, 9 Horndasch M....Gloy J. (2006)
    3. CHOP is involved in endoplasmic reticulum stress-induced apoptosis by enhancing DR5 expression in human carcinoma cells. (PubMed id 15322075)1, 2, 9 Yamaguchi H. and Wang H.G. (2004)
    4. TRB3, a novel ER stress-inducible gene, is induced via ATF4-CHOP pathway and is involved in cell death. (PubMed id 15775988)1, 2, 9 Ohoka N....Hayashi H. (2005)
    5. Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma. (PubMed id 8510758)1, 2, 9 Crozat A.... Ron D. (1993)
    6. Induction by ionizing radiation of the gadd45 gene in cultured human cells: lack of mediation by protein kinase C. (PubMed id 1990262)1, 3, 9 Papathanasiou M.A.... Fornace A.J. Jr. (1991)
    7. Endoplasmic reticulum stress-induced transcription fa ctor, CHOP, is crucial for dendritic cell IL-23 expression. (PubMed id 20876114)1, 2 Goodall J.C....Gaston J.S. (2010)
    8. Endoplasmic reticulum stress-activated C/EBP homologo us protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma. (PubMed id 20829347)1, 2 Park S.H....Moon Y. (2010)
    9. Adaptive suppression of the ATF4-CHOP branch of the u nfolded protein response by toll-like receptor signalling. (PubMed id 19855386)1, 2 Woo C.W....Tabas I. (2009)
    10. ER stress-inducible factor CHOP affects the expressio n of hepcidin by modulating C/EBPalpha activity. (PubMed id 19672300)1, 2 Oliveira S.J....de Almeida S.F. (2009)

    External Searches for DDIT3 gene

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing DDIT3 gene

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 1649 HGNC: 2726 AceView: DDIT3 Ensembl:ENSG00000175197 euGenes: HUgn1649
    ECgene: DDIT3 Kegg: 1649 H-InvDB: DDIT3

    Other Databases showing DDIT3 gene

    (According to HUGE)
    About This Section
    		   --

    Specialized Databases showing DDIT3 gene

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for DDIT3 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for DDIT3 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/ddit3/

    Intellectual Property
    for DDIT3 gene

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    		Patent Information for DDIT3 gene:
    Search GeneIP for patents involving DDIT3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    Products
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    About This Section

     
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