Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



DDIT3 Gene

protein-coding   GIFtS: 68
GCID: GC12M057910

DNA-Damage-Inducible Transcript 3

  See DDIT3-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
DNA-Damage-Inducible Transcript 31 2     CHOP-102 3
C/EBP Zeta1 2 3     DDIT-32 3
CHOP102 3 5     CCAAT/Enhancer-Binding Protein Homologous Protein2 3
GADD1532 3 5     CEBPZ2
C/EBP-Homologous Protein 102 3     DNA Damage-Inducible Transcript 3 Protein2
Growth Arrest And DNA Damage-Inducible Protein GADD1532 3     C/EBP-Homologous Protein3
CHOP2 3     

External Ids:    HGNC: 27261   Entrez Gene: 16492   Ensembl: ENSG000001751977   OMIM: 1263375   UniProtKB: P356383   

Export aliases for DDIT3 gene to outside databases

Previous GC identifers: GC12M059181 GC12M058053 GC12M057627 GC12M056196 GC12M054948


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for DDIT3 Gene:
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The
protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as
C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in
adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of
this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins
in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms
with different length have been identified. (provided by RefSeq, Aug 2010)

GeneCards Summary for DDIT3 Gene:
DDIT3 (DNA-damage-inducible transcript 3) is a protein-coding gene. Diseases associated with DDIT3 include myxoid liposarcoma, and colon cancer. GO annotations related to this gene include transcription factor binding and sequence-specific DNA binding transcription factor activity.

UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638
Function: Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a
wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual
role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes.
Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family,
impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP
regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34,
BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent
transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of
hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG).
Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and
repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the
induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases
increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response

Gene Wiki entry for DDIT3 (DNA damage-inducible transcript 3) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000012.11  NC_018923.2  NT_029419.13  
Regulatory elements:
   Regulatory transcription factor binding sites in the DDIT3 gene promoter:
         Max1   PPAR-gamma1   PPAR-gamma2   c-Myc   C/EBPalpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidDDIT3 promoter sequence
   Search Chromatin IP Primers for DDIT3

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat DDIT3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.1-q13.2   Ensembl cytogenetic band:  12q13.3   HGNC cytogenetic band: 12q13.1-q13.2

DDIT3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
DDIT3 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M057910:  view genomic region     (about GC identifiers)

Start:
57,910,371 bp from pter      End:
57,914,300 bp from pter
Size:
3,930 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638 (See protein sequence)
Recommended Name: DNA damage-inducible transcript 3 protein  
Size: 169 amino acids; 19175 Da
Subunit: Heterodimer. Interacts with TCF7L2/TCF4, EP300/P300, HDAC1, HDAC5 and HDAC6. Interacts with TRIB3 which
blocks its association with EP300/P300. Interacts with FOXO3, CEBPB and ATF4
Sequence caution: Sequence=AAB27103.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of
unknown origin in the N-terminal part;
Secondary accessions: F8VS99
Alternative splicing: 2 isoforms:  P35638-1   P35638-2   

Explore the universe of human proteins at neXtProt for DDIT3: NX_P35638

Explore proteomics data for DDIT3 at MOPED

Post-translational modifications: 

  • Ubiquitinated, leading to its degradation by the proteasome1
  • Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while
    phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity (By similarity)1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See DDIT3 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (6 alternative transcripts): 
    NP_001181982.1  NP_001181983.1  NP_001181984.1  NP_001181985.1  NP_001181986.1  NP_004074.2  

    ENSEMBL proteins: 
     ENSP00000447188   ENSP00000448665   ENSP00000340671   ENSP00000447803   ENSP00000447503  
    Reactome Protein details: P35638

    DDIT3 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for DDIT3
    OriGene Custom MassSpec
    OriGene Custom Protein Services for DDIT3
    GenScript Custom Purified and Recombinant Proteins Services for DDIT3
    Novus Biologicals DDIT3 Proteins
    Novus Biologicals DDIT3 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for DDIT3
    Cloud-Clone Corp. Proteins for DDIT3

     
    Search eBioscience for Proteins for DDIT3 

    DDIT3 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for DDIT3 (GADD153)
    Cell Signaling Technology (CST) Antibodies for DDIT3  (CHOP)
    OriGene Antibodies for DDIT3
    OriGene Custom Antibody Services for DDIT3
    Novus Biologicals DDIT3 Antibodies
    Abcam antibodies for DDIT3
    Cloud-Clone Corp. Antibodies for DDIT3
    ThermoFisher Antibody for DDIT3
    LSBio Antibodies in human, mouse, rat for DDIT3

    DDIT3 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for DDIT3
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for DDIT3
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for DDIT3
    Cloud-Clone Corp. CLIAs for DDIT3
    Search eBioscience for ELISAs for DDIT3 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    2 InterPro protein domains:
     IPR016670 DNA_damage_induc_transcript_3
     IPR004827 bZIP

    Graphical View of Domain Structure for InterPro Entry P35638

    ProtoNet protein and cluster: P35638

    UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638
    Domain: The N-terminal region is necessary for its proteasomal degradation, transcriptional activity and
    interaction with EP300/P300
    Similarity: Belongs to the bZIP family
    Similarity: Contains 1 bZIP (basic-leucine zipper) domain


    Find genes that share domains with DDIT3           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: DDIT3_HUMAN, P35638
    Function: Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a
    wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual
    role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes.
    Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family,
    impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP
    regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34,
    BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent
    transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of
    hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG).
    Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and
    repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the
    induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases
    increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response
    Induction: By oxidative stress, amino-acid deprivation, hypoxia and ER stress. During ER stress, induced by a
    EIF2AK3/ATF4 pathway and/or ERN1/ATF6 pathway. Expression is suppressed by TLR-TRIF signaling pathway during
    prolonged ER stress

         Genatlas biochemistry entry for DDIT3:
    DDIT3 dominant negative regulator of several C/EBP transcription factors,involved in t(12;16) translocation (see
    FUS),involved in erythroid growth and differentiation possibly through interaction with both C/EBP and non C/EBP
    family members

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IDA14667815
    GO:0003700sequence-specific DNA binding transcription factor activity IEA--
    GO:0003714transcription corepressor activity TAS7503811
    GO:0005515protein binding IPI15775988
    GO:0008134transcription factor binding IPI16434966
         
    Find genes that share ontologies with DDIT3           About GenesLikeMe


    Phenotypes:
         3 GenomeRNAi human phenotypes for DDIT3:
     Decreased NANOG protein expres  Decreased OCT4 protein express  Decreased POU5F1-GFP protein e 

         7 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Ddit3):
     behavior/neurological  cellular  endocrine/exocrine gland  hematopoietic system  homeostasis/metabolism 
     immune system  nervous system 

    Find genes that share phenotypes with DDIT3           About GenesLikeMe

    Animal Models:
         MGI mouse knock-outs for DDIT3: Ddit3tm1Mori Ddit3tm1Dron

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for DDIT3
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for DDIT3

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for DDIT3
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for DDIT3

    Transcription Factor Targeting: 
    Selected GeneGlobe predicted Target genes for DDIT3 (see all 372):
    AARS2,  ABCA10,  ACSF3,  ACYP2,  ADAL,  ADAM21,  ADAM33,  ADAMTS18,  ADC,  ALKBH1

    Targeting motifs: HOMER Transcription Factor Regulatory Elements motif viewer 
                                          Consensus sequence:  ATTGCATCAT 

    miRNA
    Products:
        
    miRTarBase miRNAs that target DDIT3:
    hsa-mir-96-5p (MIRT027903), hsa-mir-335-5p (MIRT016927)

    Block miRNA regulation of human, mouse, rat DDIT3 using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate DDIT3:
    hsa-miR-4314 hsa-miR-1271 hsa-miR-96 hsa-miR-3153
    SwitchGear 3'UTR luciferase reporter plasmidDDIT3 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for DDIT3
    Predesigned siRNA for gene silencing in human, mouse, rat DDIT3

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for DDIT3

    Clone
    Products:
         
    OriGene clones in human, mouse for DDIT3 (see all 11)
    OriGene ORF clones in mouse, rat for DDIT3
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 6): DDIT3 (NM_001195053)
    Sino Biological Human cDNA Clone for DDIT3
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DDIT3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DDIT3
    Addgene plasmids for DDIT3 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for DDIT3
    Browse ESI BIO Cell Lines and PureStem Progenitors for DDIT3 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DDIT3

    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA1-10126) for DDIT3 


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    DDIT3_HUMAN, P35638: Cytoplasm. Nucleus. Note=Present in the cytoplasm under non-stressed conditions and ER
    stress leads to its nuclear accumulation
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    endoplasmic reticulum4
    cytosol2
    mitochondrion2
    extracellular1

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm TAS--
    GO:0005770late endosome IEA--

    Find genes that share ontologies with DDIT3           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for DDIT3 About   (see all 17)  
    See pathways by source

    SuperPathContained pathways About
    1MAPK signaling pathway
    MAPK signaling pathway0.50
    MAPK signaling pathway0.50
    2Unfolded Protein Response
    Unfolded Protein Response0.61
    Activation of Chaperone Genes by ATF6-alpha0.00
    Activation of Chaperones by ATF6-alpha0.00
    3PERK regulated gene expression
    PERK regulated gene expression0.90
    Activation of Genes by ATF40.90
    4Akt Signaling
    p38 Signaling0.60
    5ERK Signaling
    MAPK Signaling0.58


    Find genes that share SuperPaths with DDIT3           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    2 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for DDIT3
        MAPK Signaling
    p38 Signaling

    1 Cell Signaling Technology (CST) Pathway for DDIT3
        Translational Control

    1 GeneGo (Thomson Reuters) Pathway for DDIT3
        Development Growth hormone signaling via PI3K/AKT and MAPK cascades

    Selected BioSystems Pathways for DDIT3 (see all 6)
        MAPK signaling pathway
    Adipogenesis
    p38 MAPK Signaling Pathway
    ATF-2 transcription factor network
    Signaling mediated by p38-alpha and p38-beta


    2 Reactome Pathways for DDIT3
        ATF6-alpha activates chaperone genes
    ATF4 activates genes

    1 PharmGKB Pathway for DDIT3
        Celecoxib Pathway, Pharmacodynamics

    4 Kegg Pathways  (Kegg details for DDIT3):
        MAPK signaling pathway
    Protein processing in endoplasmic reticulum
    Non-alcoholic fatty liver disease (NAFLD)
    Transcriptional misregulation in cancer

        Pathway & Disease-focused RT2 Profiler PCR Arrays including DDIT3 (see all 11): 
              DNA Damage Signaling Pathway in human mouse rat
              Cancer PathwayFinder in human mouse rat
              Stress & Toxicity PathwayFinder in human mouse rat
              Adipogenesis in human mouse rat
              cAMP / Ca2+ Signaling PathwayFinder in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for DDIT3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for DDIT3 (P356382, 3 ENSP000003406714) via UniProtKB, MINT, STRING, and/or I2D (see all 420)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 29):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001955blood vessel maturation IEA--
    GO:0006355regulation of transcription, DNA-templated IMP17872950
    GO:0006915apoptotic process ----
    GO:0006974cellular response to DNA damage stimulus TAS1339368
    GO:0006983ER overload response IEA--

    Find genes that share ontologies with DDIT3           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for DDIT3

    Selected Novoseek inferred chemical compound relationships for DDIT3 gene (see all 64)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tunicamycin 80.2 26 15775988 (3), 18228003 (2), 17825316 (1), 15605392 (1) (see all 10)
    n-(4-hydroxyphenyl)retinamide 75.8 58 12353227 (8), 12234979 (6), 12138118 (5), 15917187 (5) (see all 10)
    l-(4,5-3h)leucine 71.7 1 11113529 (1)
    thapsigargin 65.6 22 15322075 (3), 9365549 (2), 11389688 (1), 18266951 (1) (see all 11)
    pga2 62.7 12 1735464 (6), 7926370 (1)
    methylmethanesulfonate 59.8 7 8892753 (2), 8754828 (1), 9586958 (1), 1400365 (1)
    n acetylcysteine 54.2 21 19722195 (2), 8670069 (1), 15271854 (1), 9044846 (1) (see all 12)
    sp 600125 53.4 6 15466475 (1), 15680548 (1), 18767967 (1), 19139269 (1)
    indole-3-carbinol 45.4 1 15681163 (1)
    deferoxamine mesylate 44.1 2 14604972 (1)



    Find genes that share compounds with DDIT3           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for DDIT3 gene (6 alternative transcripts): 
    NM_001195053.1  NM_001195054.1  NM_001195055.1  NM_001195056.1  NM_001195057.1  NM_004083.5  

    Unigene Cluster for DDIT3:

    DNA-damage-inducible transcript 3
    Hs.505777  [show with all ESTs]
    Unigene Representative Sequence: NM_001195056
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000547303 ENST00000551116(uc001soi.3 uc009zpt.3 uc021qzj.1 uc021qzk.1 uc021qzl.1 uc009zps.3)
    ENST00000346473 ENST00000552740 ENST00000547526
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat DDIT3 using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate DDIT3:
    hsa-miR-4314 hsa-miR-1271 hsa-miR-96 hsa-miR-3153
    SwitchGear 3'UTR luciferase reporter plasmidDDIT3 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for DDIT3
    Predesigned siRNA for gene silencing in human, mouse, rat DDIT3
    Clone
    Products:
         
    OriGene clones in human, mouse for DDIT3 (see all 11)
    OriGene ORF clones in mouse, rat for DDIT3
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 6): DDIT3 (NM_001195053)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for DDIT3
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DDIT3
    Addgene plasmids for DDIT3 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for DDIT3
    OriGene qSTAR qPCR primer pairs in human, mouse for DDIT3
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat DDIT3
      QuantiTect SYBR Green Assays in human, mouse, rat DDIT3
      QuantiFast Probe-based Assays in human, mouse, rat DDIT3
    Flow Cytometry
    Products:
       

     
    eBioscience FlowRNA Probe Sets ( VA1-10126) for DDIT3 

    Additional mRNA sequence: 

    AK316581.1 AY461580.1 BC003637.2 BT006691.1 S40706.1 

    14 DOTS entries:

    DT.91780645  DT.100045496  DT.97810576  DT.95193903  DT.100687083  DT.121187388  DT.40306179  DT.91780612 
    DT.40236699  DT.100687093  DT.91780636  DT.91968162  DT.121187409  DT.95259608 

    Selected AceView cDNA sequences (see all 277):

    R51042 AW302469 CD678684 BU664077 BQ128379 BM823128 BQ183476 BM830935 
    NM_004083 BM823630 BM831126 BQ694049 CN482753 AA128240 CB144081 BQ721406 
    BX509791 BQ019188 BQ224643 BM841910 CN482428 BU664440 BM669449 BU682572 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for DDIT3 (see all 8)    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b · 3c · 3d · 3e · 3f ^ 4a · 4b · 4c · 4d · 4e
    SP1:              -           -     -     -     -                                             
    SP2:              -           -     -     -                                                   
    SP3:              -                                                                           
    SP4:              -           -                                                               
    SP5:              -           -     -     -     -     -                                       


    ECgene alternative splicing isoforms for DDIT3

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    DDIT3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AACAATTGGG
    DDIT3 Expression
    About this image


    DDIT3 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 3) fully expand
     
     Limb (Muscoskeletal System)
             Limb Bud Mesenchyme Cells Limb Bud
     
     Blood (Hematopoietic System)
             Granulocytes Peripheral Blood
     
     Mesoderm (Gastrulation Derivatives)
             Limb Bud Mesenchyme Cells Limb Bud
    DDIT3 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    DDIT3 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.505777
        Pathway & Disease-focused RT2 Profiler PCR Arrays including DDIT3 (see all 11): 
              DNA Damage Signaling Pathway in human mouse rat
              Cancer PathwayFinder in human mouse rat
              Stress & Toxicity PathwayFinder in human mouse rat
              Adipogenesis in human mouse rat
              cAMP / Ca2+ Signaling PathwayFinder in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for DDIT3
    OriGene qSTAR qPCR primer pairs in human, mouse for DDIT3
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat DDIT3
    QuantiTect SYBR Green Assays in human, mouse, rat DDIT3
    QuantiFast Probe-based Assays in human, mouse, rat DDIT3
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DDIT3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of chordates.

    Orthologs for DDIT3 gene from Selected species (see all 8)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Ddit31 , 5 DNA-damage inducible transcript 31, 5 87.7(n)1
    88.69(a)1
      10 (74.50 cM)5
    131981  NM_007837.31  NP_031863.31 
     1272907745 
    lizard
    (Anolis carolinensis)
    Reptilia DDIT36
    DNA-damage-inducible transcript 3
    45(a)
    1 ↔ 1
    GL343212.1(570984-579441)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia ddit31 DNA-damage-inducible transcript 3 56.5(n)
    51.83(a)
      733816  NM_001045579.1  NP_001039044.1 
    zebrafish
    (Danio rerio)
    Actinopterygii ddit36
    DNA-damage-inducible transcript 3
    32(a)
    1 ↔ 1
    6(58818530-58821727) ENSDARG00000059836


    ENSEMBL Gene Tree for DDIT3 (if available)
    TreeFam Gene Tree for DDIT3 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
      --

    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    2 SNPs for DDIT3    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0360004
    A colorectal cancer sample4--see VAR_0360002 A V mis40--------
    rs3761995891,2
    ----57915265(+) ACAAA-/CAAA  
            
    AAAAC
    6 -- us2k10--------

    HapMap Linkage Disequilibrium report for DDIT3 (57910371 - 57914300 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 7 variations for DDIT3:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv469411CNV Loss19166990
    nsv899119CNV Loss21882294
    nsv899120CNV Loss21882294
    nsv899117CNV Loss21882294
    nsv899118CNV Gain21882294
    nsv428283CNV Gain18775914
    dgv513e1CNV Complex17122850

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing DDIT3
    DNA2.0 Custom Variant and Variant Library Synthesis for DDIT3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 126337   
    OMIM disorders: 613488  
    UniProtKB/Swiss-Prot: DDIT3_HUMAN, P35638
  • Myxoid liposarcoma (MXLIPO) [MIM:613488]: A soft tissue tumor that tends to occur in the limbs
    (especially the thigh) of patients ranging in age from 35 to 55 years. It is defined by the presence of a
    hypocellular spindle cell proliferation set in a myxoid background, often with mucin pooling. Lipoblasts tend to
    be small and often monovacuolated and to cluster around vessels or at the periphery of the lesion. Note=The gene
    represented in this entry may be involved in disease pathogenesis. A chromosomal aberration involving DDIT3 has
    been found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS
    (PubMed:7503811)

  • 2 diseases for DDIT3:    
    About MalaCards
    myxoid liposarcoma    colon cancer

    1 disease from the University of Copenhagen DISEASES database for DDIT3:
    Liposarcoma

    Find genes that share disorders with DDIT3           About GenesLikeMe

    Selected Novoseek inferred disease relationships for DDIT3 gene (see all 36)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    liposarcoma myxoid 91.3 26 18162764 (3), 8143276 (2), 8637704 (2), 8510758 (2) (see all 17)
    liposarcoma 79.1 29 11126370 (2), 12168660 (2), 9676855 (1), 12169678 (1) (see all 18)
    sarcoma 45.1 8 18162764 (2), 7687873 (2), 8117620 (1), 10448063 (1)
    cancer 39.9 22 15322075 (3), 16463383 (2), 12138118 (2), 19276161 (1) (see all 12)
    lipoma 34.2 2 9244875 (1), 20332486 (1)
    colon cancer 32.9 6 18534616 (1), 15271854 (1), 16434966 (1), 17180111 (1) (see all 6)
    ewings sarcoma 31.7 2 7493135 (1), 20332486 (1)
    tumors 31.6 31 10430059 (4), 10803921 (3), 10534579 (2), 8117620 (2) (see all 20)
    cell damage 25.6 2 11113529 (2)
    malignant fibrous histiocytoma 22.5 1 8143276 (1)

    Genatlas disease: DDIT3
    myxoid liposarcoma,with breakpoint in t(12;16) translocation (see FUS)

    Genetic Association Database (GAD): DDIT3
    Human Genome Epidemiology (HuGE) Navigator: DDIT3 (4 documents)

    Export disorders for DDIT3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for DDIT3 gene, integrated from 10 sources (see all 430):
    (articles sorted by number of sources associating them with DDIT3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Critical and functional regulation of CHOP (C/EBP homologous protein) through the N-terminal portion. (PubMed id 17872950)1, 2, 9 Ohoka N.... Hayashi H. (J. Biol. Chem. 2007)
    2. The C/EBP homologous protein CHOP (GADD153) is an inhibitor of Wnt/TCF signals. (PubMed id 16434966)1, 2, 9 Horndasch M.... Gloy J. (Oncogene 2006)
    3. CHOP is involved in endoplasmic reticulum stress-induced apoptosis by enhancing DR5 expression in human carcinoma cells. (PubMed id 15322075)1, 2, 9 Yamaguchi H. and Wang H.G. (J. Biol. Chem. 2004)
    4. TRB3, a novel ER stress-inducible gene, is induced via ATF4-CHOP pathway and is involved in cell death. (PubMed id 15775988)1, 2, 9 Ohoka N.... Hayashi H. (EMBO J. 2005)
    5. Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma. (PubMed id 8510758)1, 2, 9 Crozat A.... Ron D. (Nature 1993)
    6. Induction by ionizing radiation of the gadd45 gene in cultured human cells: lack of mediation by protein kinase C. (PubMed id 1990262)1, 3, 9 Papathanasiou M.A.... Fornace A.J. Jr. (Mol. Cell. Biol. 1991)
    7. Endoplasmic reticulum stress-induced transcription factor, CHOP, is crucial for dendritic cell IL-23 expression. (PubMed id 20876114)1, 2 Goodall J.C....Gaston J.S. (Proc. Natl. Acad. Sci. U.S.A. 2010)
    8. Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma. (PubMed id 20829347)1, 2 Park S.H.... Moon Y. (J. Biol. Chem. 2010)
    9. Adaptive suppression of the ATF4-CHOP branch of the unfolded protein response by toll-like receptor signalling. (PubMed id 19855386)1, 2 Woo C.W.... Tabas I. (Nat. Cell Biol. 2009)
    10. ER stress-inducible factor CHOP affects the expression of hepcidin by modulating C/EBPalpha activity. (PubMed id 19672300)1, 2 Oliveira S.J.... de Almeida S.F. (PLoS ONE 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 1649 HGNC: 2726 AceView: DDIT3 Ensembl:ENSG00000175197 euGenes: HUgn1649
    ECgene: DDIT3 Kegg: 1649 H-InvDB: DDIT3

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for DDIT3 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for DDIT3 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/ddit3/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for DDIT3 gene:
    Search GeneIP for patents involving DDIT3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Antibodies for DDIT3 (GADD153)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for DDIT3   OriGene RNAi products in human, mouse, rat for DDIT3  
     OriGene qPCR primer pairs and template standards for DDIT3   OriGene Protein Over-expression Lysate for DDIT3  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for DDIT3  
     OriGene qSTAR qPCR primer pairs in human, mouse for DDIT3   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for DDIT3   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for DDIT3   OriGene Custom Protein Services for DDIT3  

     
     
     Block miRNA regulation of human, mouse, rat DDIT3 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing DDIT3
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat DDIT3 Predesigned siRNA for gene silencing in human, mouse, rat DDIT3
     QuantiFast Probe-based Assays in human, mouse, rat DDIT3 QuantiTect SYBR Green Assays in human, mouse, rat DDIT3
     PCR Arrays including human, mouse, rat DDIT3 Search Chromatin IP Primers for DDIT3
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat DDIT3  GeneGlobe Interaction Network for DDIT3
     Regulatory tfbs in DDIT3 promoter
     GenScript Custom Purified and Recombinant Proteins Services for DDIT3 GenScript cDNA clones with any tag delivered in your preferred vector for DDIT3
     GenScript Custom Assay Services for DDIT3 GenScript Custom overexpressing Cell Line Services for DDIT3
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Antibodies & Assays for DDIT3  (CHOP)

     Search Tocris compounds for DDIT3
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for DDIT3
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     DDIT3 antibodies
     DDIT3 proteins
     DDIT3 lysates
     Antibodies for DDIT3
     See all of Abcam's Antibodies, Kits and Proteins for DDIT3
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Recombinant Protein for DDIT3
     Proteins for DDIT3
     Antibodies for DDIT3
     ELISAs for DDIT3
     CLIAs for DDIT3



     Browse ESI BIO Cell Lines and PureStem Progenitors for DDIT3
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DDIT3
     SwitchGear 3'UTR luciferase reporter plasmids for DDIT3
     SwitchGear Promoter luciferase reporter plasmids for DDIT3
     ThermoFisher Antibody for DDIT3
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DDIT3
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for DDIT3
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for DDIT3
     LSBio Antibodies in human, mouse, rat for DDIT3
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Addgene plasmids for DDIT3
      Search eBioscience for proteins for DDIT3
      Search eBioscience for elisas for DDIT3
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      DDIT3 gene at Home site.
    Version: 3.12.212 17 Sep 2014
    hostname: 356977-web1.xennexinc.com index build: 128 solr: 1.4