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DCTN4 Gene

protein-coding   GIFtS: 51

GC05M150068
dynactin 4 (p62)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Descriptions
Dynactin subunit p62 3
dynactin 4 (p62) 2
dynactin p62 subunit 2
External Ids
HGNC: 155181
Entrez Gene: 511642
UniProtKB: Q9UJW03
Ensembl: ENSG000001329127
Search outside databases for aliases for DCTN4 gene

Previous GC identifers: GC05M150157 GC05M150685 GC05M150116

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: DCTN4_HUMAN, Q9UJW0
Function: Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin
pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in
linking dynein and dynactin to the cortical cytoskeleton

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DCTN4 gene  

Entrez Gene cytogenetic band: 5q31-q32   Ensembl cytogenetic band:  5q33.1   HGNC cytogenetic band: 5q31-q32

DCTN4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M150068:     (about GC identifiers)

Start:
150,068,502 bp from pter
End:
150,118,769 bp from pter
Size:
50,268 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000005.8  NT_029289.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: DCTN4_HUMAN, Q9UJW0 (See protein sequence)
Recommended Name: Dynactin subunit 4  
Size: 460 amino acids; 52337 Da
Subunit: Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4,
DCTN5 and DCTN6 subunits and ACTR10 (By similarity). Binds directly to the ACTR1A subunit of
dynactin
Subcellular location: Cytoplasm, cytoskeleton. Centrosome. Note=Has a punctate cytoplasmic
distribution as well as centrosomal distribution typical of dynactin. Overexpression does not
disrupt microtubule organization or the integrity of the Golgi but does cause both cytosolic and
nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high
expression levels
Secondary accessions: Q8TAN8

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (3 alternative transcripts): 
    NP_001129115.1  NP_001129116.1  NP_057305.1  


    ENSEMBL proteins: 
    ENSP00000255263 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (DCTN4)
    Browse Abnova for Human Recombinant Proteins
                  OriGene Purified Recombinant Human Protein: DCTN4 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus TAS10671518
    GO:0005737 cytoplasm TAS10671518
    GO:0005813 centrosome TAS10671518
    GO:0005856 cytoskeleton IEA--
    About this table

    Antibodies for DCTN4: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (DCTN4), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for DCTN4

    Assays for DCTN4: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR008603 Dynactin_p62


       GeneDecks  DCTN4 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UJW0

    ProtoNet protein and cluster: Q9UJW0

    UniProtKB/Swiss-Prot: DCTN4_HUMAN, Q9UJW0
    Similarity: Belongs to the dynactin subunit 4 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DCTN4)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_016221

                  Applied Biosystems Silencer® siRNAs for DCTN4

                  Sigma-Aldrich siRNA for DCTN4  
                         Sigma-Aldrich shRNA Panels and shRNA for DCTN4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001135643
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001135643
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_001135643 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001135643

    UniProtKB/Swiss-Prot: DCTN4_HUMAN, Q9UJW0
    Function: Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin
    pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in
    linking dynein and dynactin to the cortical cytoskeleton

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0047485 protein N-terminus binding IPI16554302
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    3 Millipore Pathways for DCTN4
     Cell cycle Spindle assembly and chromosome separation
     Cytoskeleton remodeling Neurofilaments
     Cytoplasmic microtubules

       GeneDecks  DCTN4 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for DCTN4):
     hsa05016 Huntington's disease

       GeneDecks  DCTN4 for the pathways selected above  
    About GeneDecksing

    3 Interacting proteins for DCTN4 (Q9UJW01 ENSP000002552633) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ANK2Q014841, ENSP000003495883EBI-2134033, EBI-941975 STRING (score=.859)
    ACTR1AENSP000003589213STRING (score=.873)
    ATP7BENSP000002428393STRING (score=.829)
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DCTN4

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DCTN4)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_016221

                  Sigma-Aldrich siRNA for DCTN4  
                         Sigma-Aldrich shRNA Panels and shRNA for DCTN4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001135643  NM_001135644  NM_016221  

    REFSEQ mRNAs for DCTN4 gene (3 alternative transcripts): 

    NM_001135643.1   NM_001135644.1   NM_016221.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001135643  NM_001135644  NM_016221  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_001135643
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_001135643
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_001135643 

    Additional cDNA sequence: 

    AF156165.2 AF195120.1 AK000299.1 AK125973.1 AK296787.1 AK299517.1 AK307757.1 AK311693.1 
    AL162085.1 BC026323.1 

    17 DOTS entries:

    DT.416781  DT.99944395  DT.91771117  DT.95235369  DT.100788049  DT.101963558  DT.120818940  DT.100788047 
    DT.120818933  DT.91771115  DT.120818949  DT.100840837  DT.95156429  DT.120818977  DT.91771116  DT.91805447 
    DT.95311210 

    24/300 AceView cDNA sequences (see all 300 ):

    AL162085 AA771783 AA352583 BG750279 AW089524 CA442898 AK125973 BE218028 
    AA054471 BG571610 BG149433 AW160363 T16286 AI873466 BI963887 AI276307 
    AW043971 BQ051358 BM927713 BF445266 BG253621 AI073775 AI985890 CA394492 

    highest scoring ESTs for DCTN4:

    AA223368 AA323250 AA582253 AF195120 AK000299 AL597070 AL703548 AW015575 BC026323 BE885675 

    Unigene Cluster for DCTN4:

    Dynactin 4 (p62)
    Hs.675564  [show with all ESTs]
    Unigene Representative Sequence: NM_001135644


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000255263  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DCTN4 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DCTN4

    1 / 2 / 3

    7 probe-sets matching DCTN4 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    43847_s_at2, 3 U95-B 1 1.00 1.00 0.89 1.15 AA582253 0.80 1.00 0.91 1

    83502_at2, 3 U95-E 1 1.00 1.00 0.86 0.68 AI248742 0.60 1.00 0.82 1

    65917_at2, 3 U95-C 1 1.00 1.00 0.76 1.18 AW015575 0.80 1.00 0.91 1

    218013_x_at2, 3 U133-A 1 1.00 1.00 -- -- NM_016221 0.60 1.00 0.82 1

    222488_s_at2, 3 U133-B 1 1.00 1.00 -- -- BE218028 0.60 1.00 0.82 1

    218013_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    222488_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CATATTTGTG

    SOURCE GeneReport for Unigene cluster: Hs.675564
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DCTN4 gene from 5/11 species (see all 11 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DCTN41   -- dynactin 4 (p62) 93.26(n)
    98.7(a)
    479326  XM_848442.1  XP_853535.1 
    chimpanzee
    (Pan troglodytes)
    DCTN41   -- dynactin 4 (p62) 99.71(n)
    99.78(a)
    462195  XM_001167059.1  XP_001167059.1 
    cow
    (Bos taurus)
    DCTN41   -- dynactin 4 (p62) 94.28(n)
    98.04(a)
    613489  XM_875085.2  XP_880178.2 
    rat
    (Rattus norvegicus)
    Dctn41   -- dynactin 4 89.86(n)
    96.74(a)
    84428  NM_053404.1  NP_445856.1 
    mouse
    (Mus musculus)
    Dctn41, 5 185
    dynactin 41, 5 89.64(n)1
    96.74(a)1
    676651  NM_026302.31  NP_080578.11 
     AI0359725  AI5515255  (see all 30)
    About this table        Species with no ortholog for DCTN4

    ENSEMBL Gene Tree for DCTN4
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/280 NCBI SNPs in DCTN4 are shown (see all 280 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 96)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 5 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs119546521,2
    A,C,F,H150078076(+) CACTCG/CAAGAG 3 L/F mis113Minor allele frequency- C:0.28EA NA EU WA 1106
    rs105156421,2
    A,C,F,H150068963(+) AGTTAG/ATTTGG 3 -- ut31 ese316Minor allele frequency- A:0.14NS EU EA WA NA 1084
    rs100712361,2
    A,C,F,H150120333(+) CAATAG/ACCTGG 1 -- ng3111Minor allele frequency- A:0.21EU EA WA NA 980
    rs2521581,2
    A,C,F,H150068080(-) ACACTC/GAAGCT 1 -- ng5112Minor allele frequency- G:0.30EU EA WA 1258
    rs93246511,2
    A,C,F,H150119253(+) TTAGCG/ATTAGG 1 -- ng317Minor allele frequency- A:0.25EU EA WA NA 560
    rs77060891,2
    C,F,H150118827(+) TCCCTA/GCGACT 2 -- ut5110Minor allele frequency- C:0.01NA MN EA EU WA 1886
    rs37339251,2
    C,F,H150070537(-) ATGTCA/GCAGAT 3 -- ut31 ese39Minor allele frequency- G:0.06EA EU WA 2280
    rs37339241,2
    C,F,H150070573(-) GTACTG/ATCTCT 3 -- ut31 ese311Minor allele frequency- A:0.07EA EU WA 2502
    rs37339231,2
    C,F,H150070974(-) AGAAAG/ATGACC 3 N/S mis115Minor allele frequency- A:0.06EA EU WA 2918
    rs104769411,2
    C,F,H150068274(+) CTAACA/GACATT 1 -- ng514Minor allele frequency- G:0.16EU EA WA 416
    About this table

    HapMap Linkage Disequilibrium images for DCTN4 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    8 PubMed articles for DCTN4 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 51164 HGNC: 15518 AceView: DCTN4 Ensembl:ENSG00000132912 euGenes: HUgn51164
    ECgene: DCTN4 H-InvDB: DCTN4
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DCTN4:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DCTN4-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for DCTN4 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for DCTN4 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Purified proteins from HEK293T cells
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     shRNA in GFP-retroviral vector
     Search Tocris compounds for DCTN4




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     Search Novus for antibodies for DCTN4

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