DCTN3 Gene
protein-coding GIFtS: 56
GCID: GC09M034614
|
|
dynactin 3 (p22)
| |
Aliases
for DCTN3 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Dynactin 3 (P22)1 2 | | P221 | | DCTN222 3 5 | | Dynactin 32 | | DCTN-221 2 | | Dynactin Light Chain2 | | Dynactin Complex Subunit 22 KDa Subunit2 3 | | Dynactin Subunit 32 |
Export aliases for DCTN3 gene to outside databasesPrevious GC identifers: GC09M034925 GC09M034782 GC09M034598 GC09M034603 GC09M034569 |
Summaries
for DCTN3 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for DCTN3:
This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in sizefrom 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array ofcellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindleformation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most otherdynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with verylittle coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this genegenerates 2 transcript variants. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: DCTN3_HUMAN, O75935Function: Together with dynein may be involved in spindle assembly and cytokinesis
Gene Wiki entry for DCTN3
|
Genomic Views
for DCTN3 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000009.11 NC_018920.1 NT_008413.18
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the DCTN3 gene promoter:
HOXA9 HOXA9B p53 Brachyury Nkx2-5 Nkx5-1 Meis-1b HNF-1 Meis-1
Other transcription factors
Search SABiosciences Chromatin IP Primers for DCTN3
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DCTN3 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 9p13 Ensembl cytogenetic band: 9p13.3 HGNC cytogenetic band: 9p13DCTN3 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 9 GeneLoc Exon Structure GeneLoc location for GC09M034614: view genomic region
(about GC identifiers)
Start:
|
34,613,548 bp from pter |
End:
|
34,620,515 bp from pter |
Size:
|
6,968 bases |
Orientation:
|
minus strand |
|
Proteins
for DCTN3 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: DCTN3_HUMAN, O75935 (See
protein sequence)Recommended Name: Dynactin subunit 3 Size: 186 amino acids; 21119 Da
Subunit: Subunit of dynactin, a multiprotein complex associated with dynein
Subcellular location: Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Cytoplasm,cytoskeleton, spindle. Cleavage furrow. Midbody. Note=Localizes to punctate cytoplasmic structures and to thecentrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Colocalizes with dynein tothe cleavage furrow and to midbody of dividing cells
Sequence caution: Sequence=CAI13144.1; Type=Erroneous initiation; Sequence=CAI14178.1; Type=Erroneous gene modelprediction; Sequence=CAI14180.1; Type=Erroneous initiation;
Secondary accessions: A6NII7 B2RBM5 Q5T1I5 Q5T1I7 Q5T8G3 Q9BPU8Alternative splicing: 3 isoforms: O75935-1 O75935-2 O75935-3 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for DCTN3: NX_O75935
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O75935
DCTN3 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_009165.1 NP_077324.1
ENSEMBL proteins: ENSP00000390842 ENSP00000259632 ENSP00000343986 ENSP00000432915 ENSP00000368193 ENSP00000368191 ENSP00000368196 ENSP00000397046 Reactome Protein details: O75935
Human Recombinant Protein Products:
Gene Ontology (GO): 5/9 cellular component terms (GO ID links to tree view) (see all 9): About this table
DCTN3 for ontologies About GeneDecksing
DCTN3 Antibody Products:
Assay Products for DCTN3: |
Protein
Domains /
Families
for DCTN3 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
DCTN3 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry O75935ProtoNet protein and cluster: O75935 UniProtKB/Swiss-Prot: DCTN3_HUMAN, O75935Similarity: Belongs to the dynactin subunit 3 family |
Function
for DCTN3 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: DCTN3_HUMAN, O75935Function: Together with dynein may be involved in spindle assembly and cytokinesis
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DCTN3 (see all 5)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DCTN3 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DCTN3 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table
DCTN3 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for DCTN3:
|
Pathways & Interactions
for DCTN3 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways - 5/6 super-pathways (see all 6) About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Recruitment of mitotic centrosome proteins and complexes | | | 2 | Cytoskeleton remodeling Neurofilaments | | | 3 | Cell cycle_Spindle assembly and chromosome separation | | | 4 | Immune System | | | 5 | Cell Cycle | |
Pathway sources
See GeneCards unified pathways
Show all pathways
3 EMD Millipore Pathways for DCTN3
2  GeneGo (Thomson Reuters) Pathways for DCTN3
5/12 
Reactome Pathways for DCTN3 (see all 12)
DCTN3 for pathways About GeneDecksing
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for DCTN3
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/80 Interacting proteins for DCTN3 (O759351, 2, 3 ENSP000002596324) via UniProtKB, MINT, STRING, and/or I2D (see all 80)About this table
Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6): About this table
DCTN3 for ontologies About GeneDecksing
|
Drugs & Compounds
for DCTN3 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for DCTN3
Search CenterWatch for drugs/clinical trials and news about DCTN3
|
Transcripts
for DCTN3 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for DCTN3 gene (2 alternative transcripts): NM_007234.3 NM_024348.2 Unigene Cluster for DCTN3: Dynactin 3 (p22) Hs.511768 [show with all ESTs]Unigene Representative Sequence: BM92063812 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000472418 ENST00000479399 ENST00000421919 ENST00000259632 ENST00000341694(uc003zuw.1)
ENST00000477738 ENST00000481438 ENST00000472074 ENST00000378913 ENST00000378911
ENST00000378916 ENST00000447983(uc003zux.1)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DCTN3 (see all 5)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DCTN3 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): DCTN3 (NM_007234) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for DCTN3 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DCTN3 |
Additional cDNA sequence: AF082513.1 AK314730.1 BC000319.2 BC003004.1 BC107697.1 CR541889.1 12 DOTS entries: DT.444493 DT.95254845 DT.100655527 DT.75190318 DT.100784036 DT.100029170 DT.95174060 DT.97845255 DT.121184527 DT.121184538 DT.92430386 DT.65288339 24/238 AceView cDNA sequences (see all 238): BQ637048 BF219701 AI420578 AA622255 AI418088 AA211786 BQ639534 BQ675889 AI014635 BE266040 AL119167 BU158801 BQ020026 BQ683093 BM915956 AA973565 BQ428250 BU075737 BM457889 BQ674338 CA312470 BU165114 BM545651 AA563604
GeneLoc Exon Structure
5/11 Alternative Splicing Database (ASD) splice patterns (SP) for DCTN3 (see all 11) About this scheme
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | ^ | 2 | ^ | 3a | · | 3b | ^ | 4 | ^ | 5a | · | 5b | · | 5c | ^ | 6a | · | 6b | ^ | 7a | · | 7b | · | 7c | ^ | 8a | · | 8b | · | 8c | · | 8d | · | 8e |
|---|
|
| SP1: | | | | | | | | | | | | | - | | | | - | | - | | - | | | | - | | - | | - | | | | - | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | - | | | | - | | - | | - | | | | - | | | | | | | | - | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | - | | | | - | | - | | - | | | | - | | - | | | | | | - | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | - | | - | | - | | - | | - | | | | - | | - | | - | | | | - | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | - | | | | - | | - | | - | | | | - | | - | | - | | - | | | | | | | | | | | |
ECgene alternative splicing isoforms for DCTN3
|
Expression
for DCTN3 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| DCTN3 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CTGCCCGCCT
About this image
See DCTN3 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for DCTN3
SOURCE GeneReport for Unigene cluster: Hs.511768
UniProtKB/Swiss-Prot: DCTN3_HUMAN, O75935Tissue specificity: Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels inbrain
SABiosciences Custom PCR Arrays for DCTN3
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DCTN3
Browse OriGene validated miRNA SYBR primer pairs
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Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DCTN3 |
Orthologs
for DCTN3 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for DCTN3 gene from 5/18 species (see all 18) About this table
ENSEMBL Gene Tree for DCTN3 (if available)
TreeFam Gene Tree for DCTN3 (if available) |
Paralogs
for DCTN3 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for DCTN3 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 9 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for DCTN3 (34613548 - 34620515 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for DCTN3
1 CNV: 9615 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DCTN3 |
|
Disorders
/ Diseases
for DCTN3 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
DCTN3 for disorders About GeneDecksing
OMIM gene information: 607387
OMIM disorders: --
3  diseases for DCTN3: About MalaCardsprostate cancer ataxia prostatitis
Export disorders for DCTN3 gene to outside databases
|
Publications
for DCTN3 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for DCTN3 gene, integrated from 9 sources (see all 24):
(articles sorted by number of sources associating them with DCTN3) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells. (PubMed id 9722614)1, 2, 3 Karki S.... Holzbaur E.L.F. (1998)
- Assignment of p22 dynactin light chain (DCTN3) to human chromosome region 9p13 by radiation hybrid mapping. (PubMed id 11306820)1, 3, 9 Mills D.R. and Jackson C.L. (2001)
- DNA sequence and analysis of human chromosome 9. (PubMed id 15164053)1, 2 Humphray S.J.... Dunham I. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
- Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
- A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)1 Kristensen A.R....Foster L.J. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
|
External Searches for DCTN3 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing DCTN3 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing DCTN3 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing DCTN3 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for DCTN3 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for DCTN3 gene:
Search GeneIP for patents involving DCTN3
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for DCTN3 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| |
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| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DCTN3 |
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