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DCBLD2 Gene

protein-coding   GIFtS: 56

GC03M099997
discoidin, CUB and LCCL domain containing 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
1700055P21Rik 2
CLCP1 1, 2, 3
ESDN 1, 2, 3
Descriptions
CUB, LCCL and coagulation factor V/VIII-homology domains
protein 1 3
Endothelial and smooth muscle cell-derived neuropilin-like
protein 2, 3
coagulation factor V/VIII-homology domains protein 1 2
discoidin, CUB and LCCL domain containing 2 2
External Ids
HGNC: 246271
Entrez Gene: 1315662
UniProtKB: Q96PD23
Ensembl: ENSG000000570197
Search outside databases for aliases for DCBLD2 gene

Previous GC identifer: GC03M099836

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Gene Wiki entry for DCBLD2
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DCBLD2 gene  

Entrez Gene cytogenetic band: 3q12.1|3   Ensembl cytogenetic band:  3q12.1   HGNC cytogenetic band: 3q12.2

DCBLD2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M099997:     (about GC identifiers)

Start:
99,997,504 bp from pter
End:
100,103,223 bp from pter
Size:
105,720 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000003.10  NT_005612.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: DCBD2_HUMAN, Q96PD2 (See protein sequence)
Recommended Name: Discoidin, CUB and LCCL domain-containing protein 2 precursor  
Size: 775 amino acids; 85035 Da
Subcellular location: Membrane; Single-pass type I membrane protein
Secondary accessions: Q8N6M4 Q8TDX2
Alternative splicing: 2 isoforms:  Q96PD2-1   Q96PD2-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_563615.3  

    ENSEMBL proteins: 
    ENSP00000385656 ENSP00000321573 ENSP00000321646 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
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    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (DCBLD2)
    Human Recombinant Proteins from Abnova (DCBLD2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005887 integral to plasma membrane ISS--
    GO:0009986 cell surface ISS--
    GO:0016020 membrane IEA--
    About this table

    Antibodies for DCBLD2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for DCBLD2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (DCBLD2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (DCBLD2)
    Novus Biologicals Antibodies for DCBLD2

    Assays for DCBLD2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR000859 CUB
     IPR000421 Coagulation_factor_5/8-type_C
     IPR004043 LCCL


       GeneDecks  DCBLD2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q96PD2

    ProtoNet protein and cluster: Q96PD2

    2 Blocks protein families:
    IPB000421 Coagulation factor 5/8 type C domain (FA58C)
    IPB000859 CUB domain


    UniProtKB/Swiss-Prot: DCBD2_HUMAN, Q96PD2
    Similarity: Contains 1 CUB domain
    Similarity: Contains 1 F5/8 type C domain
    Similarity: Contains 1 LCCL domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DCBLD2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_080927

                  Applied Biosystems Silencer® siRNAs for DCBLD2

                  Sigma-Aldrich siRNA and siRNA Panels for DCBLD2  
                         Sigma-Aldrich shRNA for DCBLD2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_080927
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_080927
                                     untagged cDNA clone in CMV expression vector: NM_080927 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_080927

    UniProtKB/Swiss-Prot: DCBD2_HUMAN, Q96PD2
    Induction: Increased in lung cancers during the process of tumor progression

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding NAS11447234
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    1 Interacting protein for DCBLD2 (ENSP000003215733) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SEMA4BENSP000003322043STRING (score=.931)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007155 cell adhesion IEA--
    GO:0030308 negative regulation of cell growth ISS--
    GO:0030522 intracellular receptor-mediated signaling pathway NAS11447234
    GO:0042060 wound healing ISS--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DCBLD2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DCBLD2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_080927

                  Sigma-Aldrich siRNA and siRNA Panels for DCBLD2  
                         Sigma-Aldrich shRNA for DCBLD2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_080927  

    REFSEQ mRNAs for DCBLD2 gene: 

    NM_080927.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_080927  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_080927
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_080927
                                     untagged cDNA clone in CMV expression vector: NM_080927 

    Additional cDNA sequence: 

    AB073146.1 AF104313.1 AF387547.1 AK001362.1 AK056350.1 AK302649.1 BC007117.1 BC016815.1 
    BC029658.1 BX647140.1 D29810.1 

    12 DOTS entries:

    DT.100028579  DT.102833519  DT.95179834  DT.75123629  DT.92056600  DT.99974173  DT.102833520  DT.120914541 
    DT.40257901  DT.416993  DT.86848429  DT.97765306 

    24/278 AceView cDNA sequences (see all 278 ):

    AI695876 BU753325 AW138866 AA128681 AI220745 AI802905 AI242761 BU171889 
    AA782668 BU628593 AA885289 AV682179 AI499115 NM_080927 AI656521 BM468461 
    BM984345 CB144123 AI379718 BU631293 AF387547 AI654989 BE708542 BU687616 

    highest scoring ESTs for DCBLD2:

    AI262981 AI220745 AI433228 AI864669 AW300360 AW611673 BG433690 BG722378 H80005 H85099 

    Unigene Cluster for DCBLD2:

    Discoidin, CUB and LCCL domain containing 2
    Hs.203691  [show with all ESTs]
    Unigene Representative Sequence: NM_080927


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for DCBLD2

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14a · 14b ^ 15a · 15b ^ 16a · 16b · 16c
    SP1:                                                                                      -     -           -                           
    SP2:                                                                                      -     -                                       
    SP3:                                                                                                                                    
    SP4:                                                                                                        -                           

    About this scheme

    ECgene alternative splicing isoforms for DCBLD2

    3 Ensembl transcripts including schematic representations:
    ENST00000404023  ENST00000326840  ENST00000326857  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DCBLD2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DCBLD2

    1 / 2 / 3

    11 probe-sets matching DCBLD2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    85153_at2, 3 U95-E 1 1.00 1.00 0.85 0.85 W24316 0.80 1.00 0.91 1
    44747_at2, 3 U95-B 1 1.00 1.00 0.89 0.95 H98103 0.80 1.00 0.91 1
    52262_at2, 3 U95-B 1 1.00 1.00 0.87 1.04 AI433228 0.80 1.00 0.91 1

    80154_r_at2, 3 U95-D 1 0.94 1.00 0.30 1.45 AI262981 1.00 1.00 1.00 1
    40227_at2, 3 U95-A 1 0.81 1.00 -0.34 0.73 D29810 0.80 0.80 0.80 1

    224911_s_at2, 3 U133-B 1 1.00 1.00 -- -- AA722799 0.40 1.00 0.76 1

    213865_at2, 3 U133-A 1 1.00 1.00 -- -- AI378788 0.60 1.00 0.82 1

    239446_x_at2, 3 U133-B 1 1.00 1.00 -- -- AW300360 0.80 1.00 0.91 1

    224911_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    213865_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    239446_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  DCBLD2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TACTGTAGTC

    SOURCE GeneReport for Unigene cluster: Hs.203691

    Expression variation in blood from EXPOLDB for DCBLD2

    UniProtKB/Swiss-Prot: DCBD2_HUMAN, Q96PD2
    Tissue specificity: Highly expressed in testis, heart, skeletal muscle and also in cultured
    vascular smooth muscle cells

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DCBLD2 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DCBLD21   -- discoidin, CUB and LCCL domain containing 2 89.61(n)
    92.58(a)
    487949  XM_545071.2  XP_545071.2 
    chimpanzee
    (Pan troglodytes)
    DCBLD21   -- discoidin, CUB and LCCL domain containing 2 99.61(n)
    99.61(a)
    460548  XM_001141399.1  XP_001141399.1 
    cow
    (Bos taurus)
    DCBLD21   -- discoidin, CUB and LCCL domain containing 2 89.55(n)
    91.48(a)
    524608  XM_602937.3  XP_602937.3 
    rat
    (Rattus norvegicus)
    Dcbld21   -- discoidin, CUB and LCCL domain containing 2 85.92(n)
    86.05(a)
    155696  NM_130419.1  NP_569103.1 
    mouse
    (Mus musculus)
    Dcbld21, 5 165
    discoidin, CUB and LCCL domain containing 21, 5 85.61(n)1
    85.14(a)1
    733791  NM_028523.31  NP_082799.21 
     AF3875485  AF4428205  (see all 17)
    About this table        Species with no ortholog for DCBLD2

    ENSEMBL Gene Tree for DCBLD2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for DCBLD2 gene
    DCBLD12  RS12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/660 NCBI SNPs in DCBLD2 are shown (see all 660 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 171)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs88331,2
    A,C,F,H99998259(+) ATTTAC/TAGCAT 1 -- ut319Minor allele frequency- T:0.39MN NA EU EA WA 924
    rs10621961,2
    A,C,F,H99999703(-) GTCCTA/GTATGT 1 -- ut31 ese39Minor allele frequency- G:0.23MN EU EA WA NA 2226
    rs24546731,2
    A,C,F,H100103809(-) TTATTC/TTAACT 1 -- ng31 tfbs38Minor allele frequency- T:0.36EU EA WA 836
    rs172709861,2
    C,F,H100000762(+) TTAGTA/GGTTTC 1 -- ut31 ese312Minor allele frequency- G:0.12NA EU EA WA 1056
    rs7045861,2
    C,F,H99997851(-) AAAGTC/TAACTG 1 -- ut31 ese35Minor allele frequency- T:0.38MN EU EA WA 566
    rs168402081,2
    C,F,H100001067(+) GCTGTC/TAGTCC 1 D/N mis1 ese311Minor allele frequency- T:0.02NA EU EA WA 982
    rs8286071,2
    C,F,H99999671(+) GCTAGG/TAAGCT 1 -- ut31 ese38Minor allele frequency- T:0.02MN EU EA WA NA 746
    rs37961331,2
    C,F,H100000533(-) ACGCAC/TGGTAC 1 -- ut31 ese35Minor allele frequency- T:0.03EA EU WA 1892
    rs115529781,2
    C,F,H100000258(-) CTCATC/TCTTGC 1 -- ut314Minor allele frequency- T:0.03EU EA WA 420
    rs18271581,2
    C,F,H100104355(-) GCTTTC/TGACTA 1 -- ng318Minor allele frequency- T:0.13EU EA WA 838
    About this table

    HapMap Linkage Disequilibrium images for DCBLD2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 608698

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/11 PubMed articles for DCBLD2 gene (see all 11 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 131566 HGNC: 24627 AceView: DCBLD2 Ensembl:ENSG00000057019 euGenes: HUgn131566
    ECgene: DCBLD2 H-InvDB: DCBLD2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for DCBLD2 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DCBLD2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DCBLD2-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for DCBLD2 Antibodies for DCBLD2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
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