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DCBLD1 Gene

protein-coding GIFtS: 46

GC06P117910

discoidin, CUB and LCCL domain containing 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
DKFZp686L21135 ^²
MGC46341 ^¹, ²
OTTHUMP00000017084 ^²
OTTHUMP00000040398 ^²
dJ94G16.1 ^¹, ²

Descriptions
discoidin, CUB and LCCL domain containing 1 ^²

External Ids
HGNC: 21479¹
Entrez Gene: 285761²
UniProtKB: Q8N8Z6³
Ensembl: ENSG00000164465⁷

Search outside databases for aliases for DCBLD1 gene

Previous GC identifers: GC06P117803 GC06P117849

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the DCBLD1 gene

Entrez Gene cytogenetic band: 6q22.2 Ensembl cytogenetic band: 6q22.2 HGNC cytogenetic band: 6q22.31

DCBLD1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6 GeneLoc Exon Structure

GeneLoc location for GC06P117910: (about GC identifiers)

Start:	117,910,513 bp from pter
End:	117,997,714 bp from pter
Size:	87,202 bases
Orientation:	plus strand

RefSeq DNA sequence:

NC_000006.10 NT_025741.14

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: DCBD1_HUMAN, Q8N8Z6 (See protein sequence)

Recommended Name: Discoidin, CUB and LCCL domain-containing protein 1 precursor

Size: 715 amino acids; 77920 Da

Subcellular location: Membrane; Single-pass type I membrane protein (Potential)

Sequence caution: Sequence=BAC04663.1; Type=Frameshift; Positions=483; Sequence=CAI43159.1;

Type=Erroneous gene model prediction; Sequence=CAI43171.1; Type=Erroneous gene model prediction;

Secondary accessions: Q5H992 Q8IYK5 Q8N7L9 Q96NH2

Post-translational modifications:

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_775945.1

ENSEMBL proteins:

ENSP00000296955 ENSP00000342422 ENSP00000357489 ENSP00000376292

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
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	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0016020	membrane	IEA	--
GO:0016021	integral to membrane	IEA	--

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Antibodies for DCBLD1:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for DCBLD1

Assays for DCBLD1:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry Q8N8Z6

ProtoNet protein and cluster: Q8N8Z6

2 Blocks protein families:

IPB000421 Coagulation factor 5/8 type C domain (FA58C)

IPB000859 CUB domain

UniProtKB/Swiss-Prot: DCBD1_HUMAN, Q8N8Z6

Similarity: Contains 1 CUB domain

Similarity: Contains 1 F5/8 type C domain

Similarity: Contains 1 LCCL domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (DCBLD1)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_173674

Applied Biosystems Silencer® siRNAs for DCBLD1

Sigma-Aldrich siRNA and siRNA Panels for DCBLD1

Sigma-Aldrich shRNA for DCBLD1

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_173674
Myc/DDK tagged cDNA clone in CMV expression vector: NM_173674
untagged cDNA clones in CMV expression vector (see all 2): AK095973

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_173674

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Interacting protein for DCBLD1 (Q8N8Z6²) via UniProtKB, MINT, and/or STRING
Interactant Interaction Details
GeneCard External ID(s)
PDIA3 P30101² MINT-4301418
About this table
1 Gene Ontology (GO) biological process term (links to tree view):
GO ID Qualified GO term Evidence PubMed IDs
GO:0007155 cell adhesion IEA --
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for DCBLD1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (DCBLD1)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_173674

Sigma-Aldrich siRNA and siRNA Panels for DCBLD1

Sigma-Aldrich shRNA for DCBLD1

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_173674

REFSEQ mRNAs for DCBLD1 gene:

NM_173674.1

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_173674

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_173674
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_173674
                                 untagged cDNA clones in CMV expression vector (see all 2): AK095973

Additional cDNA sequence:

AJ420528.1 AJ420597.1 AK055462.1 AK095973.1 AK098194.1 BC035671.1 CR749448.1

8 DOTS entries:

DT.113027 DT.97781862 DT.70104320 DT.91743937 DT.102826917 DT.121324007 DT.91975878 DT.92016795

24/94 AceView cDNA sequences (see all 94 ):

BM558496 BU615199 AL048059 AL045008 AA570058 BI324930 NM_173674 BC035671

BM805721 AI672550 AI568958 BX108857 AI423986 AJ420597 CF593883 BM996157

AI867398 BX355226 BF221704 CB960471 AI492223 AI968039 AI912501 AI968060

highest scoring ESTs for DCBLD1:

AI866358 AI935615 BC035671 N22751 W31518 AA026810 AA026871 AA400528 AA431749 AA470837

Unigene Cluster for DCBLD1:

Discoidin, CUB and LCCL domain containing 1

Hs.658304 [show with all ESTs]

Unigene Representative Sequence: CR749448

GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for DCBLD1

ExUns:	1	^	2a	·	2b	^	3	^	4	^	5	^	6	^	7	^	8a	·	8b	^	9	^	10a	·	10b	^	11a	·	11b	^	12	^	13	^	14a	·	14b	^	15	^	16a	·	16b
SP1:							-										-																		-
SP2:																																			-
SP3:
SP4:
SP5:

About this scheme

ECgene alternative splicing isoforms for DCBLD1

4 Ensembl transcripts including schematic representations:

ENST00000296955 ENST00000338728 ENST00000368503 ENST00000392504

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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DCBLD1 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for DCBLD1

¹ / ² / ³

6 probe-sets matching DCBLD1 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
69467_at²^{, 3}	U95-D	1	1.00	1.00	0.63	0.90	W31518	0.80	1.00	0.91	1
64103_at²^{, 3}	U95-C	1	1.00	1.00	0.91	1.20	N22751	0.80	1.00	0.91	1
64326_r_at²^{, 3}	U95-C	1	0.94	1.00	0.87	0.91	AI866358	0.80	1.00	0.91	1
226609_at²^{, 3}	U133-B	1	1.00	1.00	--	--	N22751	0.80	1.00	0.91	1
1553768_a_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
226609_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

GeneDecks DCBLD1 for binary patterns associated with the probe-sets selected above

About GeneDecksing

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Data from

(Publications) and GNF BioGPS
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About these images

CGAP SAGE TAG: GTTTTATGCG

SOURCE GeneReport for Unigene cluster: Hs.658304

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
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Orthologs for DCBLD1 gene from 5/7 species (see all 7 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	DCBLD1¹	--	discoidin, CUB and LCCL domain containing 1	87.5(n) 87.5(a)	484099 XM_541216.2 XP_541216.2
chimpanzee (Pan troglodytes)	DCBLD1¹	--	discoidin, CUB and LCCL domain containing 1	99.27(n) 99.6(a)	472109 XM_527488.2 XP_527488.2
rat (Rattus norvegicus)	Dcbld1¹	--	discoidin, CUB and LCCL domain containing 1	81.48(n) 81.87(a)	309773 XM_228172.4 XP_228172.4
mouse (Mus musculus)	Dcbld1^{1, 5}	10⁵	discoidin, CUB and LCCL domain containing 1⁵ discoidin, CUB and LCCL domain containing 1¹	83.84(n)¹ 81.82(a)¹	66686¹ NM_025705.2¹ NP_079981.1¹ AA414451⁵ AK016485⁵ (see all 9)
chicken (Gallus gallus)	DCBLD1¹	--	discoidin, CUB and LCCL domain containing 1	71.19(n) 69.41(a)	421734 XM_419767.2 XP_419767.2

About this table Species with no ortholog for DCBLD1

ENSEMBL Gene Tree for DCBLD1

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for DCBLD1 gene

DCBLD2² RS1²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
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10/404 NCBI SNPs in DCBLD1 are shown (see all 404 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 180)

AB	SNP ID	Valid	Chr 6 pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs4946258^1,2	A,C,F,H	117910231(+)	`CACCAC/TGCGGC`	1	--	ng5¹		4		EU EA WA	416
	rs17079281^1,2	C,F,H	117909831(+)	`TGCCAC/TTTAGG`	1	--	ng5¹		12		NA EA EU WA	1054
	rs4946257^1,2	A,C	117909333(+)	`CTCCAC/GGCTTC`	1	--	ng5¹		0	--	--	--	--
--	rs13204101^1,2	H	117968842(+)	`ATGAGA/CCAATC`	1	T/P	mis¹ ese³		4		EU EA WA	406
	rs9285421^1,2	A,C,F,H	117949391(+)	`ATAACA/GTGTTT`	1	T/T	syn¹		13		EU EA WA NS NA	712
	rs35230921^1,2	F	117968616(-)	`TGCCAT/CGTCTG`	1	T/T	syn¹		1		NA	72
	rs210609^1,2	A,C,F,H,O	117940054(-)	`TTCACT/AACCCA`	1	--	int¹		11		EU EA WA NA	980
	rs210617^1,2	A,C,F,H,O	117966751(-)	`CAACTA/GCGAGT`	1	--	int¹ ese³		8		MN EU EA WA NA	744
	rs210625^1,2	A,C,F,H,O	117980950(-)	`CACAGC/TGTGAA`	1	--	int¹		17		MN EU EA WA NA	1596
	rs2057314^1,2	A,C,F,H,O	117926050(-)	`TGAAGC/TTTAGA`	1	--	int¹		13		MN EA NA EU WA	1234

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HapMap Linkage Disequilibrium images for DCBLD1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
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(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

4 PubMed articles for DCBLD1 gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{3, 4} Ota T....Sugano S. (2004)
The DNA sequence and analysis of human chromosome 6. (PubMed id 14574404)^{3, 4} Mungall A.J.... Beck S. (2003)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 285761	HGNC: 21479	AceView: DCBLD1	Ensembl:ENSG00000164465	euGenes: HUgn285761
ECgene: DCBLD1	H-InvDB: DCBLD1

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Licensable Technologies for DCBLD1
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for DCBLD1:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for DCBLD1-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for DCBLD1		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for DCBLD1		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

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	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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	Custom Service for Rabbit Pab from Full-length Protein

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Search Tocris compounds for DCBLD1

Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for DCBLD1

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DCBLD1 Gene

protein-coding GIFtS: 46

Aliases &
Descriptions
for DCBLD1

Summaries
for DCBLD1

Genomic Location
for DCBLD1

Proteins
for DCBLD1

Protein Domains/
Families
for DCBLD1

Gene Function
for DCBLD1

Pathways & Interactions
for DCBLD1

Drugs & Compounds
for DCBLD1

Transcripts
for DCBLD1

Expression
for DCBLD1

Orthologs
for DCBLD1

Paralogs
for DCBLD1

SNPs/Variants
for DCBLD1

Disorders & Mutations
for DCBLD1

Medical News
for DCBLD1

Publications
for DCBLD1

Search for DCBLD1

Genome Databases showing DCBLD1

Other Databases showing DCBLD1

Specialized Databases showing DCBLD1

Licensable Technologies
for DCBLD1

Services
for DCBLD1

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

DCBLD1 Gene

protein-coding GIFtS: 46

Aliases & Descriptionsfor DCBLD1

Summariesfor DCBLD1

Genomic Locationfor DCBLD1

Proteinsfor DCBLD1

Protein Domains/Familiesfor DCBLD1

Gene Functionfor DCBLD1

Pathways & Interactionsfor DCBLD1

Drugs & Compoundsfor DCBLD1

Transcriptsfor DCBLD1

Expression for DCBLD1

Orthologsfor DCBLD1

Paralogsfor DCBLD1

SNPs/Variantsfor DCBLD1

Disorders & Mutationsfor DCBLD1

Medical Newsfor DCBLD1

Publicationsfor DCBLD1

Search for DCBLD1

Genome Databases showing DCBLD1

Other Databases showing DCBLD1

Specialized Databases showing DCBLD1

Licensable Technologiesfor DCBLD1

Servicesfor DCBLD1

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for DCBLD1

Summaries
for DCBLD1

Genomic Location
for DCBLD1

Proteins
for DCBLD1

Protein Domains/
Families
for DCBLD1

Gene Function
for DCBLD1

Pathways & Interactions
for DCBLD1

Drugs & Compounds
for DCBLD1

Transcripts
for DCBLD1

Expression
for DCBLD1

Orthologs
for DCBLD1

Paralogs
for DCBLD1

SNPs/Variants
for DCBLD1

Disorders & Mutations
for DCBLD1

Medical News
for DCBLD1

Publications
for DCBLD1

Licensable Technologies
for DCBLD1

Services
for DCBLD1