DCAF12 Gene
protein-coding GIFtS: 45
GCID: GC09M034079
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|
DDB1 and CUL4 associated factor 12(Previous names: KIAA1892, WD repeat domain 40A )
(Previous symbols: KIAA1892, WDR40A)
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Aliases
for DCAF12 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| DDB1 And CUL4 Associated Factor 121 2 | | Testis Cancer Centrosome-Related Protein2 3 | | KIAA18921 2 3 | | WD Repeat-Containing Protein 40A2 3 | | TCC521 2 3 | | DKFZP434O1251 | | WDR40A1 2 3 | | MGC10581 | | CT1021 2 | | Cancer/Testis Antigen 1022 | | WD Repeat Domain 40A1 2 | | DDB1- And CUL4-Associated Factor 122 | | Centrosome-Related Protein TCC522 3 | | |
Export aliases for DCAF12 gene to outside databases |
Summaries
for DCAF12 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for DCAF12:
This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complexthat interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates.(provided by RefSeq, Jul 2009)
UniProtKB/Swiss-Prot: DCA12_HUMAN, Q5T6F0Function: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex
|
Genomic Views
for DCAF12 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000009.11 NC_018920.1 NT_008413.18
Regulatory elements:
 Search SABiosciences Regulatory transcription factor binding sites for DCAF12
Other transcription factors
Search SABiosciences Chromatin IP Primers for DCAF12
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat DCAF12 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 9p13.3 Ensembl cytogenetic band: 9p13.3 HGNC cytogenetic band: 9p11.2DCAF12 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 9 GeneLoc Exon Structure GeneLoc location for GC09M034079: view genomic region
(about GC identifiers)
Start:
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34,086,381 bp from pter |
End:
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34,127,397 bp from pter |
Size:
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41,017 bases |
Orientation:
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minus strand |
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Proteins
for DCAF12 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: DCA12_HUMAN, Q5T6F0 (See
protein sequence)Recommended Name: DDB1- and CUL4-associated factor 12 Size: 453 amino acids; 50517 Da
Subunit: Interacts with DDB1
Subcellular location: Cytoplasm. Cytoplasm, cytoskeleton, centrosome
Sequence caution: Sequence=BAA91106.1; Type=Frameshift; Positions=27; Sequence=BAB67785.1; Type=Erroneous initiation;Sequence=CAI15300.1; Type=Erroneous gene model prediction; Sequence=CAI15302.1; Type=Erroneous gene model prediction;
1 PDB 3D structure from  and Proteopedia  for DCAF12:3I7P (3D)
Secondary accessions: A8KA70 D3DRL6 Q5T6E9 Q5T6F1 Q6P3V0 Q7L4F8 Q96PZ5 Q9NXA9 Q9UFJ1Explore the universe of human proteins at neXtProt for DCAF12: NX_Q5T6F0
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q5T6F0
DCAF12 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_056212.1
ENSEMBL proteins: ENSP00000355114 ENSP00000380187 ENSP00000415833
Human Recombinant Protein Products:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
DCAF12 for ontologies About GeneDecksing
DCAF12 Antibody Products:
Assay Products for DCAF12: |
Protein
Domains /
Families
for DCAF12 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
DCAF12 for domains About GeneDecksing
4 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q5T6F0ProtoNet protein and cluster: Q5T6F0 UniProtKB/Swiss-Prot: DCA12_HUMAN, Q5T6F0Similarity: Belongs to the WD repeat DCAF12 familySimilarity: Contains 4 WD repeats |
Function
for DCAF12 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: DCA12_HUMAN, Q5T6F0Function: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DCAF12 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DCAF12
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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| |  | GenScript: all cDNA clones in your preferred vector: DCAF12 (NM_015397) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DCAF12 |
|
Pathways & Interactions
for DCAF12 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
UniProtKB/Swiss-Prot: DCA12_HUMAN, Q5T6F0Pathway: Protein modification; protein ubiquitinationInteractions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for DCAF12
STRING Interaction
Network Preview (showing 5 interactants - click image to see 23)
5/27 Interacting proteins for DCAF12 (Q5T6F03 ENSP000003551144) via UniProtKB, MINT, STRING, and/or I2D (see all 27) About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016567 | protein ubiquitination |
IEA | -- |
DCAF12 for ontologies About GeneDecksing
|
Drugs & Compounds
for DCAF12 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for DCAF12
Search CenterWatch for drugs/clinical trials and news about DCAF12 / DCA12
|
Transcripts
for DCAF12 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for DCAF12 gene: NM_015397.3 Unigene Clusters for DCAF12: DDB1 and CUL4 associated factor 12 Hs.493750 [show with all ESTs], Hs.741270 [show with all ESTs]Unigene Representative Sequences: BM477163, NM_0153975 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000361264(uc003ztt.2) ENST00000466402 ENST00000396990 ENST00000450964
ENST00000463286
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for DCAF12 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for DCAF12
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: DCAF12 (NM_015397) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for DCAF12 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DCAF12 |
Additional cDNA sequence: AB067479.1 AF131771.1 AK000354.1 AK292935.1 AL117646.1 BC008893.2 BC063823.1 DQ518909.1 10 DOTS entries: DT.456182 DT.100757767 DT.100708325 DT.92057874 DT.121203710 DT.91719845 DT.100815044 DT.440635 DT.95356002 DT.97774544 24/322 AceView cDNA sequences (see all 322): AA835632 BQ425196 BQ424510 AI680758 BP374864 CD366727 AL039439 AI247498 BM772534 BU621649 AI431598 AW300712 AW673670 AA740245 BM978464 CR591883 AA383244 CR622703 AA233436 AI620401 AI074601 BU626038 AA418508 BI966162
GeneLoc Exon Structure
|
Expression
for DCAF12 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| DCAF12 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TCAAAGATAA
About this image
See DCAF12 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for DCAF12
SOURCE GeneReport for Unigene clusters: Hs.493750 Hs.741270
UniProtKB/Swiss-Prot: DCA12_HUMAN, Q5T6F0Tissue specificity: Highly expressed in lung cancer tissues and some cancer cell lines. Restricted expression in normaltestis
SABiosciences Custom PCR Arrays for DCAF12
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for DCAF12
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat DCAF12 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat DCAF12 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat DCAF12 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DCAF12 |
Orthologs
for DCAF12 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for DCAF12 gene from 6/18 species (see all 18) About this table
ENSEMBL Gene Tree for DCAF12 (if available)
TreeFam Gene Tree for DCAF12 (if available) |
Paralogs
for DCAF12 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for DCAF12 gene
- DCAF12L12 DCAF12L22
2 SIMAP similar genes for DCAF12 using alignment to 1 protein entry: DCA12_HUMAN:DCAF12L2 DCAF12L1
DCAF12 for paralogs About GeneDecksing
|
Genomic Variants
for DCAF12 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 9 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for DCAF12 (34086381 - 34127397 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 2 variations for DCAF12
1 CNV: 36949
1 Indel: 59526 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing DCAF12 |
|
Disorders
/ Diseases
for DCAF12 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
DCAF12 for disorders About GeneDecksing
1  disease for DCAF12: About MalaCardslung cancer
Export disorders for DCAF12 gene to outside databases
|
Publications
for DCAF12 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for DCAF12 gene, integrated from 9 sources (see all 22):
(articles sorted by number of sources associating them with DCAF12) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins. (PubMed id 11572484)1, 2, 3 Nagase T....Ohara O. (2001)
- Novel centrosome protein, TCC52, is a cancer-testis antigen. (PubMed id 18957058)1, 2 Li S....He D. (2008)
- A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1. (PubMed id 16949367)1, 2 Jin J.... Walter J.C. (2006)
- Large-scale concatenation cDNA sequencing. (PubMed id 9110174)1, 3 Yu W.... Gibbs R.A. (1997)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- Systematic analysis of human protein complexes identi fies chromosome segregation proteins. (PubMed id 20360068)1 Hutchins J.R....Peters J.M. (2010)
- Dynamics of cullin-RING ubiquitin ligase network revea led by systematic quantitative proteomics. (PubMed id 21145461)1 Bennett E.J....Harper J.W. (2010)
- An interaction network of the mammalian COP9 signalos ome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. (PubMed id 19295130)1 Olma M.H....Pintard L. (2009)
- Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
|
External Searches for DCAF12 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing DCAF12 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing DCAF12 gene
(According to HUGE)
About This Section
| |
Specialized Databases showing DCAF12 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for DCAF12 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for DCAF12 gene:
Search GeneIP for patents involving DCAF12
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for DCAF12 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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variant library, vector shuttling | | | OriGene Custom Antibody Services for DCAF12 | | OriGene Custom Protein Services for DCAF12 | | | OriGene Custom Immunoassay Development | | |
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| | | | Search Tocris compounds for DCAF12 |
| |  |  |  |  | | | | |
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 |
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 | | | DCAF12 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for DCAF12 |
|  |  |  | | | | ThermoFisher Antibodies for DCAF12 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat DCAF12 |
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