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DBN1 Gene

protein-coding   GIFtS: 60

GC05M176816
drebrin 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: D0S117E)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
D0S117E 2, 3
DKFZp434D064 2
drebrin-1 2
Descriptions
Developmentally-regulated brain protein 3
drebrin 1 2
drebrin E 2
drebrin E2 2
External Ids
HGNC: 26951
Entrez Gene: 16272
UniProtKB: Q166433
Ensembl: ENSG000001137587
Search outside databases for aliases for DBN1 gene

Previous GC identifers: GC05M177180 GC05M177726 GC05M176819 GC05M176865 GC05M176867

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for DBN1:
The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in
the process of neuronal growth. It is a member of the drebrin family of proteins that are
developmentally regulated in the brain. A decrease in the amount of this protein in the brain has
been implicated as a possible contributing factor in the pathogenesis of memory disturbance in
Alzheimer's disease. At least two alternative splice variants encoding different protein
isoforms have been described for this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: DREB_HUMAN, Q16643
Function: Drebrins might play some role in cell migration, extension of neuronal processes and
plasticity of dendrites, respectively

Gene Wiki entry for DBN1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DBN1 gene  

Entrez Gene cytogenetic band: 5q35.3   Ensembl cytogenetic band:  5q35.3   HGNC cytogenetic band: 5q35.3

DBN1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M176816:     (about GC identifiers)

Start:
176,816,220 bp from pter
End:
176,833,271 bp from pter
Size:
17,052 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000005.8  NT_023133.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: DREB_HUMAN, Q16643 (See protein sequence)
Recommended Name: Drebrin  
Size: 649 amino acids; 71429 Da
Subunit: Binds F-actin
Subcellular location: Cytoplasm
Secondary accessions: B2RBG0 Q9UFZ5
Alternative splicing: 2 isoforms:  Q16643-1   Q16643-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_004386.2  NP_543157.1  


    ENSEMBL proteins: 
    ENSP00000292385 ENSP00000377193 ENSP00000377195 ENSP00000308532 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Drebrin)
    Human Recombinant Proteins from Abnova (DBN1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622 intracellular IEA--
    GO:0005737 cytoplasm NAS12009525
    GO:0030425 dendrite NAS12009525
    GO:0042641 actomyosin NAS12761245
    About this table

    Antibodies for DBN1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for DBN1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Drebrin), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (DBN1)
    Novus Biologicals Antibodies for DBN1

    Assays for DBN1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR002108 Actin-bd_cofilin/tropomyosin


       GeneDecks  DBN1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q16643

    ProtoNet protein and cluster: Q16643

    1 Blocks protein family: IPB002108 Actin-binding

    UniProtKB/Swiss-Prot: DREB_HUMAN, Q16643
    Similarity: Contains 1 ADF-H domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DBN1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_080881

                  Applied Biosystems Silencer® siRNAs for DBN1

                  Sigma-Aldrich siRNA for DBN1  
                         Sigma-Aldrich shRNA Panels and shRNA for DBN1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for DBN1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_004395
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_004395
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_004395 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_080881

    UniProtKB/Swiss-Prot: DREB_HUMAN, Q16643
    Function: Drebrins might play some role in cell migration, extension of neuronal processes and
    plasticity of dendrites, respectively

    Genatlas biochemistry entry for DBN1:
    drebrin,actin binding protein involved in neuronal growth,developmentally regulated,embryonic
    isoforms E1,E2

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003779 actin binding NAS12761245
    GO:0005522 profilin binding ISS--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/10 Interacting proteins for DBN1 (ENSP000002923853 Q166431, 2) via UniProtKB, MINT, and/or STRING (see all 10 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    Gja1 Gja1 (xeno)P080501EBI-351394, EBI-476947
    IKBKGQ9Y6K92MINT-48133
    MAP3K3Q997592MINT-48378
    RIPK3Q9Y5722MINT-49084
    HOMER2ENSP000003056323STRING (score=.815)
    About this table

    5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007015 actin filament organization ISS--
    GO:0007275 multicellular organismal development IEA--
    GO:0007399 nervous system development IEA--
    GO:0030154 cell differentiation IEA--
    GO:0048168 regulation of neuronal synaptic plasticity NAS12009525
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DBN1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DBN1)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_080881

                  Sigma-Aldrich siRNA for DBN1  
                         Sigma-Aldrich shRNA Panels and shRNA for DBN1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_004395  NM_080881  

    REFSEQ mRNAs for DBN1 gene (2 alternative transcripts): 

    NM_004395.3   NM_080881.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_004395  NM_080881  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_004395
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_004395
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_004395 

    Additional cDNA sequence: 

    AK094125.1 AK225523.1 AK295295.1 AK314645.1 AL110225.1 BC000283.1 BC007281.1 BC007567.1 
    BC114553.1 D17530.1 U00802.1 

    22 DOTS entries:

    DT.456300  DT.100662726  DT.100817464  DT.97844144  DT.100027314  DT.95171424  DT.100817454  DT.100817469 
    DT.100687880  DT.100657865  DT.100817461  DT.120810927  DT.97791817  DT.100817471  DT.100733779  DT.100817467 
    DT.120810907  DT.120810958  DT.95171422  DT.95171430  DT.100663063  DT.99992448 

    24/255 AceView cDNA sequences (see all 255 ):

    AI637966 BQ954237 BI831134 BF515686 BP872369 BQ881944 BF338628 AK094125 
    T28437 BF924338 AA903534 BX952568 BM558409 AA853109 BQ918816 BE797393 
    AU141639 BQ950648 BM837519 BQ057914 BI829141 BM918417 AI904931 T08014 

    highest scoring ESTs for DBN1:

    U00802 AI978592 AK094125 AL134622 AL535268 AL556638 AU099234 AU120589 AU127414 AU129533 

    Unigene Cluster for DBN1:

    Drebrin 1
    Hs.130316  [show with all ESTs]
    Unigene Representative Sequence: NM_080881


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for DBN1 (see all 6 )

    ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15a · 15b ^ 16 ^ 17a · 17b · 17c
    SP1:                          -                                         -           -                                                   
    SP2:        -     -           -                                         -           -                                                   
    SP3:        -     -           -                                         -           -                                                   
    SP4:                                                                                -                                                   
    SP5:                                                                                                                                    

    About this scheme

    ECgene alternative splicing isoforms for DBN1

    4 Ensembl transcripts including schematic representations:
    ENST00000292385  ENST00000393563  ENST00000393565  ENST00000309007  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DBN1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DBN1

    1 / 2 / 3

    6 probe-sets matching DBN1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    63670_s_at2, 3 U95-C 1 1.00 1.00 0.97 1.11 AI978592 0.80 1.00 0.91 1
    37981_at2, 3 U95-A 1 1.00 1.00 0.98 0.89 U00802 1.00 1.00 1.00 1

    217025_s_at2, 3 U133-A 1 1.00 1.00 -- -- AL110225 0.60 1.00 0.82 1

    202806_at2, 3 U133-A 1 1.00 1.00 -- -- NM_004395 0.60 1.00 0.82 1

    217025_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    202806_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  DBN1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCAATAAATG

    SOURCE GeneReport for Unigene cluster: Hs.130316

    Expression variation in blood from EXPOLDB for DBN1

    UniProtKB/Swiss-Prot: DREB_HUMAN, Q16643
    Tissue specificity: Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and
    pancreas

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DBN1 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DBN11   -- drebrin 1 88.78(n)
    92.16(a)
    489088  XM_546204.2  XP_546204.2 
    chimpanzee
    (Pan troglodytes)
    DBN11   -- drebrin 1 99.41(n)
    99.03(a)
    462307  XM_518132.2  XP_518132.2 
    cow
    (Bos taurus)
    DBN11   -- drebrin 1 89.16(n)
    91.99(a)
    505406  NM_001045917.1  NP_001039382.1 
    rat
    (Rattus norvegicus)
    Dbn11   -- drebrin 1 85.34(n)
    88.89(a)
    81653  NM_031024.1  NP_112286.1 
    mouse
    (Mus musculus)
    Dbn11, 5 13 (34.00 cM)5
    drebrin 11, 5 86.06(n)1
    89.2(a)1
    563201  NM_019813.31  NP_062787.21 
     AA0118065  AB0287405  (see all 15)
    About this table        Species with no ortholog for DBN1

    ENSEMBL Gene Tree for DBN1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for DBN1 gene
    HCLS12  CTTN2  DBNL2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/85 NCBI SNPs in DBN1 are shown (see all 85 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 44)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 5 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs25448091,2
    A,C,F,H176818105(+) GTGGAT/CCTCCG 2 V/I mis1 ese310Minor allele frequency- C:0.45MN EU EA WA NA 1098
    rs26307631,2
    C,F176833611(-) TTAACA/TTGAGG 2 -- ng31 tfbs32Minor allele frequency- T:0.31MN EA 1252
    rs25457941,2
    C,F,H176827362(-) GTGGCG/AGAGTT 2 A/A syn1 ese34Minor allele frequency- A:0.38EU EA WA 412
    rs20291641,2
    A,C,F,H176829018(-) CCAGTC/TGTGTG 2 -- int14Minor allele frequency- T:0.11EU EA WA 420
    rs3354201,2
    A,C,F,H176831225(-) TTTCTA/GCACAA 2 -- int113Minor allele frequency- G:0.36MN EU EA WA NA 1384
    rs3354661,2
    A,C,F,H176822136(-) CGGAGG/TGGGGA 2 -- int1 trp38Minor allele frequency- T:0.37EA NA EU WA 570
    rs3373741,2
    A,C,F,H176823022(-) TAGTGC/TGTTGA 2 -- int15Minor allele frequency- T:0.43MN EU EA WA 596
    rs3354671,2
    A,C,F,H176823248(-) CAGTGT/CGGTAC 2 -- int17Minor allele frequency- C:0.35MN EU EA WA NA 778
    --
    rs3354651,2
    A,C,F176821723(-) AACAAC/TGTGTG 2 -- int11Minor allele frequency- T:0.01MN 184
    rs3354681,2
    C,F,H176824955(-) ggaatC/Tgcacg 2 -- int18Minor allele frequency- T:0.31EU EA WA 838
    About this table

    HapMap Linkage Disequilibrium images for DBN1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 126660

    5 Novoseek disease relationships for DBN1 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    down syndrome 54.52 6 12009525 (2), 15484828 (1), 15140563 (1), 11771761 (1)
    alzheimers disease 46.22 9 12009525 (2), 8838578 (2), 10374754 (1), 15484828 (1) (see all 6)
    encephalopathy 18.10 1 11771761 (1)
    dementia 0.00 1 10374754 (1)
    schizophrenia 0.00 2 16402129 (1)
    About this table

    1 PharmGKB disease relationship for DBN1 gene
    Disease PharmGKB Relations PubMed IDs for articles supporting these relationships
    Leukemia, Lymphocytic, Acute, L1FA  GN  12086872
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/85 PubMed articles for DBN1 gene (see all 85 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 1627 HGNC: 2695 AceView: DBN1 Ensembl:ENSG00000113758 euGenes: HUgn1627
    ECgene: DBN1 H-InvDB: DBN1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DBN1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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