V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

DBF4B Gene

protein-coding   GIFtS: 51

GC17P040142
DBF4 homolog B (S. cerevisiae)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
ASKL1 1, 2, 3, 5
DRF1 1, 2, 3, 5
FLJ13087 1, 2
MGC15009 2
ZDBF1B 1, 2
chifb 1, 2
Descriptions
ASK-like protein 1 3
Activator of S phase kinase-like protein 1 3
Chiffon homolog B 2, 3
DBF4 homolog B 2
DBF4 homolog B (S. cerevisiae) 2
Dbf4-related factor 1 2, 3
activator of S-phase kinase-like protein 1 2
zinc finger, DBF-type containing 1B 2
External Ids
HGNC: 178831
Entrez Gene: 801742
UniProtKB: Q8NFT63
Ensembl: ENSG000001616927
Search outside databases for aliases for DBF4B gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for DBF4B:
This gene encodes a regulator of the CDC7-like 1 protein, a serine-threonine kinase which links
cell cycle regulation to genome duplication. This protein localizes to the nucleus and its
expression is cell cycle-regulated. Alternative splicing of this gene results in two transcript
variants encoding different isoforms. Additional transcript variants have been described, but
their full length sequences have not been determined. [provided by RefSeq]

UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6
Function: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central
role in DNA replication and cell proliferation. Required for progression of S and M phases. The
complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in
regulating the initiation of DNA replication during cell cycle

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DBF4B gene  

Entrez Gene cytogenetic band: 17q21.31|17q21   Ensembl cytogenetic band:  17q21.31   HGNC cytogenetic band: 17q21.31

DBF4B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P040142:     (about GC identifiers)

Start:
40,141,502 bp from pter
End:
40,185,158 bp from pter
Size:
43,657 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000017.9  NT_010783.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6 (See protein sequence)
Recommended Name: Protein DBF4 homolog B  
Size: 615 amino acids; 67243 Da
Subunit: Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or
DBF4B. Such complexes are stable upon replication stress
Subcellular location: Nucleus. Note=Predominantly found in soluble fraction but not in the
chromatin-bound fraction
Developmental stage: Increases as cells enter in S phase through G2/M phase. The protein has a
short half-life (at protein level)
Secondary accessions: Q8TEX0 Q96B19 Q9H912
Alternative splicing: 4 isoforms:  Q8NFT6-1   Q8NFT6-2   Q8NFT6-3   Q8NFT6-4   

Post-translational modifications:

  • Phosphorylated1


  • REFSEQ proteins (2 alternative transcripts): 
    NP_079380.1  NP_663696.1  


    ENSEMBL proteins: 
    ENSP00000323663 ENSP00000377178 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (DBF4B)
    Human Recombinant Proteins from Abnova (DRF1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for DBF4B: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (DBF4B), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (DRF1)
    Novus Biologicals Antibodies for DBF4B

    Assays for DBF4B: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR006572 Znf_DBF


       GeneDecks  DBF4B for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q8NFT6

    ProtoNet protein and cluster: Q8NFT6

    UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6
    Similarity: Contains 1 BRCT domain
    Similarity: Contains 1 DBF4-type zinc finger

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DBF4B)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DRF1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): BC016158

                  Applied Biosystems Silencer® siRNAs for DBF4B

                  Sigma-Aldrich siRNA for DBF4B  
                         Sigma-Aldrich shRNA for DBF4B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for DBF4B
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_025104
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_025104
                                     untagged cDNA clones in CMV expression vector (see all 3): BC016158 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_145663

    UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6
    Function: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central
    role in DNA replication and cell proliferation. Required for progression of S and M phases. The
    complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in
    regulating the initiation of DNA replication during cell cycle

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676 nucleic acid binding IEA--
    GO:0008270 zinc ion binding IEA--
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for DBF4B
     Cell cycle Start of DNA replication in early S phase

       GeneDecks  DBF4B for the pathways selected above  
    About GeneDecksing


    3 Interacting proteins for DBF4B (ENSP000003236633 Q8NFT62) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    KRTAP4-12Q9BQ662MINT-67573
    LOC100132876Q9BQ662MINT-67573
    CDC7ENSP000003594433STRING (score=.996)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007049 cell cycle IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DBF4B

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DBF4B)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DRF1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): BC016158

                  Sigma-Aldrich siRNA for DBF4B  
                         Sigma-Aldrich shRNA for DBF4B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_025104  NM_145663  

    REFSEQ mRNAs for DBF4B gene (2 alternative transcripts): 

    NM_025104.2   NM_145663.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_025104  NM_145663  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_025104
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_025104
                                     untagged cDNA clones in CMV expression vector (see all 3): BC016158 

    Additional cDNA sequence: 

    AF448801.1 AK023149.1 AK125771.1 AK295296.1 AK303282.1 AY358101.1 BC016158.1 BC026999.1 
    BC033660.2 

    8 DOTS entries:

    DT.40283730  DT.423804  DT.40279570  DT.120983214  DT.100754986  DT.95071961  DT.120983207  DT.91729784 

    24/79 AceView cDNA sequences (see all 79 ):

    AU131166 AL042695 NM_025104 AK023149 AA622743 AK125771 BC016158 BU188746 
    BM722891 BC026999 BM476186 AF448801 AW290902 NM_145663 AW139565 AL043984 
    BU855745 BG178439 AY358101 AW886993 CK299943 BX118407 CK821286 AI003287 

    highest scoring ESTs for DBF4B:

    AA622743 AA205130 AF448801 AI144462 AK125771 AW290902 AW750787 AW886993 BC016158 BF695955 

    Unigene Cluster for DBF4B:

    DBF4 homolog B (S. cerevisiae)
    Hs.369998  [show with all ESTs]
    Unigene Representative Sequence: NM_145663


    GeneLoc Exon Structure

    5/9 Alternative Splicing Database (ASD) splice patterns (SP) for DBF4B (see all 9 )

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c · 6d ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b ^ 14a ·
    SP1:                                -     -                             -     -     -                                                                           
    SP2:                                -     -                             -     -     -                                                                           
    SP3:                                -     -                             -     -     -     -                                                                     
    SP4:                                                                    -     -     -                                   -                                       
    SP5:                                -     -                                                                                                                     

    ExUns: 14b · 14c ^ 15a · 15b ^ 16
    SP1:  -     -           -         
    SP2:  -     -                     
    SP3:                              
    SP4:                              
    SP5:                              

    About this scheme

    ECgene alternative splicing isoforms for DBF4B

    2 Ensembl transcripts including schematic representations:
    ENST00000315005  ENST00000393547  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DBF4B expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DBF4B

    1 / 2 / 3

    6 probe-sets matching DBF4B gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    79542_at2, 3 U95-D 1 1.00 1.00 1.00 1.00 AA622743 1.00 1.00 1.00 1

    238757_at2, 3 U133-B 1 1.00 1.00 -- -- AW290902 0.80 1.00 0.91 1

    206661_at2, 3 U133-A 1 1.00 1.00 -- -- NM_025104 0.60 1.00 0.82 1

    238757_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    206661_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1555378_at2 U133Plus2 2 1.00 0.82 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGCATCTTCA

    SOURCE GeneReport for Unigene cluster: Hs.369998

    UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6
    Tissue specificity: Widely expressed. Highly expressed in testis

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DBF4B gene from 3 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    DBF4B1   -- DBF4 homolog B (S. cerevisiae) 99.35(n)
    98.7(a)
    454734  XM_001147945.1  XP_001147945.1 
    chicken
    (Gallus gallus)
    DBF4B1   -- DBF4 homolog B (S. cerevisiae) 60.7(n)
    51.93(a)
    771128  XM_001234425.1  XP_001234426.1 
    African clawed frog
    (Xenopus laevis)
    AY328889.12   --   -- 75.72(n)  AY328889.1 
    About this table        Species with no ortholog for DBF4B

    ENSEMBL Gene Tree for DBF4B
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for DBF4B gene
    DBF42  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/212 NCBI SNPs in DBF4B are shown (see all 212 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 46)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 17 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs18082441,2
    A,C,F,H40165620(+) ccgagT/Cctggt 2 -- int1 ut316Minor allele frequency- C:0.35EU EA NA 438
    rs169709561,2
    C,F,H40164017(+) TCTGCA/GATATG 2 -- ut31 int112Minor allele frequency- N:0.00NA EU EA WA 1052
    rs72204621,2
    F,H40141634(+) AATCGG/CGAAGA 2 -- ut514Minor allele frequency- C:0.01EU EA WA 418
    --
    rs23378491,2
    A,C40165983(-) ctcccA/Gggttc 2 -- int1 ng310--------
    --
    rs45458691,2
    C40165903(+) aaataC/Gaaaaa 2 -- int1 ng310--------
    rs118708751,2
    A,C,F,H40161011(+) ctggaC/Tggtca 2 -- int14Minor allele frequency- T:0.13EU EA WA 420
    rs65033991,2
    A,C,F,H40149208(+) agttcG/Cctaga 2 -- int17Minor allele frequency- C:0.48EU EA WA NA 530
    rs65034021,2
    A,C,F,H40178737(+) GACTTT/GTCGAG 1 -- int111Minor allele frequency- G:0.20EU EA WA NA 976
    --
    rs29067821,2
    A,C,F,H40153274(+) GCTGAG/ATACTT 2 -- int18Minor allele frequency- A:0.09EU EA WA 838
    rs65034011,2
    A,C,F,H40175911(+) AAATAG/CGAGGG 1 -- int1 trp37Minor allele frequency- C:0.47EU EA WA NA 550
    About this table

    HapMap Linkage Disequilibrium images for DBF4B (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 611661

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/11 PubMed articles for DBF4B gene (see all 11 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 80174 HGNC: 17883 AceView: DRF1 Ensembl:ENSG00000161692 euGenes: HUgn80174
    ECgene: DBF4B H-InvDB: DBF4B
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DBF4B:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DBF4B-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for DBF4B Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for DBF4B Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
     Antibodies   Primer Pairs  
     Cell Culture Products   ELISAs  
     Flow Cytometry Kits   Protease Activity Assays & Reagents  
     Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
     Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
     Recombinant & Natural Proteins  

     Recombinant Proteins (DRF1)
     Antibodies (DRF1)
     Chimera RNAi (DRF1)
     Custom Service for Mouse Mab
     Custom Service for Rabbit Pab from Full-length Protein
      
     Search for Antibodies & Assays

     Recombinant Proteins
    (DBF4B)
     Antibodies (DBF4B)
     Search OriGene for DBF4B
     Search Tocris compounds for DBF4B




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for DBF4B

    GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

    Random Gene From GIFtS : about GIFtS

    Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

    Developed at the Crown Human Genome Center & Weizmann Institute of Science



    The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
    DBF4B Gene at Home site.
    Version 2.41.1
    server5.xennexinc.com