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Aliases & Descriptions for DBF4B
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases ASKL1 1 , 2 , 3 , 5 DRF1 1 , 2 , 3 , 5 FLJ13087 1 , 2 MGC15009 2 ZDBF1B 1 , 2 chifb 1 , 2
Descriptions ASK-like protein 1 3 Activator of S phase kinase-like protein 1 3 Chiffon homolog B 2 , 3 DBF4 homolog B 2 DBF4 homolog B (S. cerevisiae) 2 Dbf4-related factor 1 2 , 3 activator of S-phase kinase-like protein 1 2 zinc finger, DBF-type containing 1B 2
Search outside databases for aliases for DBF4B gene
Summaries for DBF4B (According to Entrez Gene ,
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Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
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EntrezGene summary for DBF4B : This gene encodes a regulator of the CDC7-like 1 protein, a serine-threonine kinase which linkscell cycle regulation to genome duplication. This protein localizes to the nucleus and itsexpression is cell cycle-regulated. Alternative splicing of this gene results in two transcriptvariants encoding different isoforms. Additional transcript variants have been described, buttheir full length sequences have not been determined. [provided by RefSeq] UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6 Function : Regulatory subunit for CDC7 which activates its kinase activity thereby playing a centralrole in DNA replication and cell proliferation. Required for progression of S and M phases. Thecomplex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved inregulating the initiation of DNA replication during cell cycle
Genomic Location for DBF4B
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the DBF4B gene Entrez Gene cytogenetic band: 17q21.31|17q21 Ensembl cytogenetic band: 17q21.31 HGNC cytogenetic band: 17q21.31 DBF4B Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 17 GeneLoc Exon Structure
GeneLoc location for GC17P040142:
(about GC identifiers )
Start:
40,141,502 bp from pter
End:
40,185,158 bp from pter
Size:
43,657 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000017.9 NT_010783.14 Proteins for DBF4B
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6 (See
protein sequence )Recommended Name: Protein DBF4 homolog B Size : 615 amino acids; 67243 Da
Subunit : Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A orDBF4B. Such complexes are stable upon replication stress
Subcellular location : Nucleus. Note=Predominantly found in soluble fraction but not in thechromatin-bound fraction
Developmental stage : Increases as cells enter in S phase through G2/M phase. The protein has ashort half-life (at protein level)
Secondary accessions : Q8TEX0 Q96B19 Q9H912Alternative splicing : 4 isoforms : Q8NFT6-1 Q8NFT6-2 Q8NFT6-3 Q8NFT6-4
Post-translational modifications:
Phosphorylated1
REFSEQ proteins (2 alternative transcripts):
NP_079380.1 NP_663696.1 ENSEMBL proteins: ENSP00000323663 ENSP00000377178 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 1 Gene Ontology (GO) cellular component term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0005634 nucleus
IEA --
About this table Antibodies for DBF4B: Assays for DBF4B:
Protein
Domains/ Families for DBF4B(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q8NFT6 ProtoNet protein and cluster: Q8NFT6
UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6 Similarity : Contains 1 BRCT domainSimilarity : Contains 1 DBF4-type zinc finger
Gene Function for DBF4B
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3 ): BC016158 Applied Biosystems Silencer ® siRNAs for DBF4B Sigma-Aldrich siRNA for DBF4B Sigma-Aldrich shRNA for DBF4B Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_025104                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_025104                                  untagged cDNA clones in CMV expression vector (see all 3 ): BC016158  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_145663 UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6 Function : Regulatory subunit for CDC7 which activates its kinase activity thereby playing a centralrole in DNA replication and cell proliferation. Required for progression of S and M phases. Thecomplex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved inregulating the initiation of DNA replication during cell cycle
3 Gene Ontology (GO) molecular function terms (links to tree view) :
About this table
Pathways & Interactions for DBF4B
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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3 Interacting proteins for DBF4B (ENSP00000323663 3 Q8NFT6 2 ) via UniProtKB, MINT, and/or STRING About this table 1 Gene Ontology (GO) biological process term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0007049 cell cycle
IEA --
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Drugs & Compounds for DBF4B (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
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Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for DBF4B
Transcripts for DBF4B(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3 ): BC016158 Sigma-Aldrich siRNA for DBF4B Sigma-Aldrich shRNA for DBF4B Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_025104 NM_145663
REFSEQ mRNAs for DBF4B gene (2 alternative transcripts): NM_025104.2 NM_145663.1
Applied Biosystems TaqMan ® Gene Expression Assays: NM_025104 NM_145663
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_025104                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_025104                                  untagged cDNA clones in CMV expression vector (see all 3 ): BC016158  
Additional cDNA sequence: AF448801.1 AK023149.1 AK125771.1 AK295296.1 AK303282.1 AY358101.1 BC016158.1 BC026999.1 BC033660.2
8 DOTS entries : DT.40283730 DT.423804 DT.40279570 DT.120983214 DT.100754986 DT.95071961 DT.120983207 DT.91729784
24/79 AceView cDNA sequences (see all 79
):AU131166 AL042695 NM_025104 AK023149 AA622743 AK125771 BC016158 BU188746 BM722891 BC026999 BM476186 AF448801 AW290902 NM_145663 AW139565 AL043984 BU855745 BG178439 AY358101 AW886993 CK299943 BX118407 CK821286 AI003287
highest scoring ESTs for DBF4B :AA622743 AA205130 AF448801 AI144462 AK125771 AW290902 AW750787 AW886993 BC016158 BF695955
Unigene Cluster for DBF4B: DBF4 homolog B (S. cerevisiae) Hs.369998 [show with all ESTs ] Unigene Representative Sequence: NM_145663 GeneLoc Exon Structure 5/9 Alternative Splicing Database (ASD) splice patterns (SP) for DBF4B (see all 9
) ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c · 6d ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b ^ 14a · SP1 :           -   -           -   -   -                           SP2 :           -   -           -   -   -                           SP3 :           -   -           -   -   -   -                         SP4 :                       -   -   -             -               SP5 :           -   -                                        
ExUns: 14b · 14c ^ 15a · 15b ^ 16 SP1 : -   -     -     SP2 : -   -         SP3 :           SP4 :           SP5 :          
About this scheme ECgene alternative splicing isoforms for DBF4B 2 Ensembl transcripts including schematic representations : ENST00000315005
ENST00000393547
Expression for DBF4B
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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DBF4B expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for DBF4B 1 / 2 / 3
6 probe-sets matching DBF4B gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: TGCATCTTCASOURCE GeneReport for Unigene cluster: Hs.369998 UniProtKB/Swiss-Prot: DBF4B_HUMAN, Q8NFT6 Tissue specificity : Widely expressed. Highly expressed in testis
Orthologs for DBF4B
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for DBF4B gene from 3 species
About this table Species with no ortholog for DBF4B ENSEMBL Gene Tree for DBF4B Paralogs for DBF4B (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for DBF4B gene DBF4 2
SNPs/Variants for DBF4B (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for DBF4B (up to first 250kb)
Disorders & Mutations for DBF4B
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 611661
Medical News for DBF4B (Possibly Related Articles in
Doctor's Guide )
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--
Publications for DBF4B (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/11 PubMed articles for DBF4B gene (see all 11
): A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required for efficient progression of S and M phases. (PubMed id 15668232) 1, 2, 3, 4 Yoshizawa-Sugata N....Masai H. (2005) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 3, 4 Ota T....Sugano S. (2004) Drf1, a novel regulatory subunit for human Cdc7 kinase. (PubMed id 12065429) 3, 4 Montagnoli A.... Santocanale C. (2002) The role of Dbf4/Drf1-dependent kinase Cdc7 in DNA-damage checkpoint control. (PubMed id 19111665) 3 Tsuji T....Jiang W. (2008) Cdc7 is an active kinase in human cancer cells undergoing replication stress. (PubMed id 17062569) 4 Tenca P....Santocanale C. (2007) Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment. (PubMed id 15782160) 3 Kim J....Iyer V.R. (2005) Towards a proteome-scale map of the human protein-protein interaction network. (PubMed id 16189514) 3 Rual J.F....Vidal M. (2005) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 4 Gerhard D.S....Malek J. (2004) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932) 3 Strausberg R.L....Marra M.A. (2002) Xenopus Drf1, a regulator of Cdc7, displays checkpoint-dependent accumulation on chromatin during an S-phase arrest. (PubMed id 12897072) 1 Yanow S.K....Dunphy W.G. (2003)
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Genome Databases showing DBF4B
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing DBF4B
(According to HUGE )
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Specialized Databases showing DBF4B (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for DBF4B (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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