 |
 | Services
| |
 |
Aliases & Descriptions for DBF4
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| ASK 1, 2, 3 | | ASKL1 5 | | CHIF 2 | | DBF4A 1, 2, 3 | | DBF4B 5 | | DRF1 5 | | OTTHUMP00000161661 2 | | ZDBF1 1, 2, 3 | | chif 1 |
| | | Descriptions |
|---|
| Activator of S phase kinase 2, 3 | | Chiffon homolog A 3 | | DBF4 homolog (S. cerevisiae) 2 | | DBF4-type zinc finger-containing protein 1 3 | | chiffon homolog 2 | | zinc finger, DBF-type containing 1 2 |
|
| | Search outside databases for aliases for DBF4 genePrevious GC identifer: GC07P087151 |
Summaries for DBF4(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: DBF4A_HUMAN, Q9UBU7Function: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycleGene Wiki entry for DBF4 |
Genomic Location for DBF4
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the DBF4 gene 
Entrez Gene cytogenetic band: 7q21.3 Ensembl cytogenetic band: 7q21.12 HGNC cytogenetic band: 7q21.3DBF4 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 7 GeneLoc Exon Structure GeneLoc location for GC07P087343:
(about GC identifiers)
Start:
|
87,343,480 bp from pter |
End:
|
87,376,792 bp from pter |
Size:
|
33,313 bases |
Orientation:
|
plus strand |
1 alternative location:
| Chr7+,CRA_TCAG 86,836,862-86,870,174 |
RefSeq DNA sequence:- NC_000007.12 NT_007933.14 NT_079595.2
| Proteins for DBF4
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: DBF4A_HUMAN, Q9UBU7 (See
protein sequence)Recommended Name: Protein DBF4 homolog A Size: 674 amino acids; 76858 Da
Subunit: Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2L, ORC4L and ORC6L
Subcellular location: Nucleus
Secondary accessions: A4D1D8 A8K954 O75226 Q75MS6 Q75N01 Q9Y2M6Alternative splicing: 2 isoforms: Q9UBU7-1 Q9UBU7-2 Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR1
View phosphorylation sites using PhosphoSite2
REFSEQ proteins: NP_006707.1
ENSEMBL proteins: ENSP00000265728
Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: DBF4 
3 Gene Ontology (GO) cellular component terms (links to tree view): About this table
Antibodies for DBF4: Assays for DBF4: | Protein
Domains/ Families for DBF4(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q9UBU7
ProtoNet protein and cluster: Q9UBU7 UniProtKB/Swiss-Prot: DBF4A_HUMAN, Q9UBU7Similarity: Contains 2 BRCT domainsSimilarity: Contains 1 DBF4-type zinc finger | Gene Function for DBF4
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006716
Applied Biosystems Silencer® siRNAs for DBF4
Sigma-Aldrich siRNA and siRNA Panels for DBF4  Sigma-Aldrich shRNA Panels and shRNA for DBF4  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006716                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_006716                                  untagged cDNA clone in CMV expression vector: NM_006716 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_006716
UniProtKB/Swiss-Prot: DBF4A_HUMAN, Q9UBU7Function: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycleInduction: Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase5 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions for DBF4
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
Gene Network CentralTM Interacting Genes and Proteins Network for DBF4 
5/45 Interacting proteins for DBF4 (Q9UBU71, 2 ENSP000002657283) via UniProtKB, MINT, and/or STRING (see all 45
)About this table
3 Gene Ontology (GO) biological process terms (links to tree view): About this table
|
Drugs & Compounds for DBF4(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for DBF4
|
Transcripts for DBF4(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006716
Sigma-Aldrich siRNA and siRNA Panels for DBF4  Sigma-Aldrich shRNA Panels and shRNA for DBF4  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_006716 REFSEQ mRNAs for DBF4 gene: NM_006716.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_006716               OriGene GFP tagged cDNA clone in CMV expression vector: NM_006716                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_006716                                  untagged cDNA clone in CMV expression vector: NM_006716  Additional cDNA sequence: AB028069.1 AB028070.1 AF160249.1 AF160876.1 AK022969.1 AK292569.1 AK302010.1 AK303175.1 BC014909.1 BC022811.1 BC035632.1 BC036045.1 BC047693.1 CR606603.1 9 DOTS entries: DT.455675 DT.100778051 DT.100697787 DT.100817855 DT.91662416 DT.121250434 DT.100817857 DT.100817860 DT.121250409 24/145 AceView cDNA sequences (see all 145
):BM854170 CB108960 BG724408 AI923577 BU175218 AF160876 AA262811 BM761973 AI149615 AA825820 BM785927 BM847871 AK022969 BC035632 AA287837 BM474158 NM_006716 AI539636 BU160067 CB123764 AV728361 AL548911 AL560180 AI630093
highest scoring ESTs for DBF4:AB028069 AA262811 AA282071 AA282335 AA451820 AA454056 AA609274 AA628515 AA648308 AA825820 Unigene Cluster for DBF4: DBF4 homolog (S. cerevisiae) Hs.485380 [show with all ESTs]Unigene Representative Sequence: NM_006716
GeneLoc Exon Structure
4 Alternative Splicing Database (ASD) splice patterns (SP) for DBF4
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8a | · | 8b | ^ | 9 | ^ | 10 | ^ | 11a | · | 11b | |
| SP1: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |
| SP3: | |   | - |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | - |   | - |   | - |   | - |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for DBF4
1 Ensembl transcript including schematic representation: ENST00000265728
|
Expression for DBF4
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| DBF4 expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for DBF4
1 / 2 / 3 3 probe-sets matching DBF4 gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: ATTCTGGTCT
SOURCE GeneReport for Unigene cluster: Hs.485380 UniProtKB/Swiss-Prot: DBF4A_HUMAN, Q9UBU7Tissue specificity: Highly expressed in testis and thymus. Expressed also in most cancer cells lines |
Orthologs for DBF4
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for DBF4 gene from 5/7 species (see all 7
)
About this table Species with no ortholog for DBF4
ENSEMBL Gene Tree for DBF4 | Paralogs for DBF4(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for DBF4 gene
- DBF4B2
Pseudogenes
- 3 related pseudogenes
|
SNPs/Variants for DBF4(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for DBF4 (up to first 250kb)
|
Disorders & Mutations for DBF4
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 611661
|
Medical News for DBF4(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for DBF4 (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/34 PubMed articles for DBF4 gene (see all 34
):- A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells. (PubMed id 10373557)2, 3, 4 Kumagai H.... Masai H. (1999)
- Functional interaction between tumor suppressor menin and activator of S-phase kinase. (PubMed id 15374998)1, 3, 4 Schnepp R.W....Hua X. (2004)
- Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication. (PubMed id 10523313)1, 3, 4 Jiang W.... Hunter T. (1999)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)3, 4 Ota T....Sugano S. (2004)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
- The DNA sequence of human chromosome 7. (PubMed id 12853948)3, 4 Hillier L.W.... Wilson R.K. (2003)
- Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. (PubMed id 10517317)2, 3 Lepke M....Grummt F. (1999)
- Human Dbf4/ASK promoter is activated through the Sp1 and MluI cell-cycle box (MCB) transcription elements. (PubMed id 12420215)1, 3 Wu X. and Lee H. (2002)
- The role of Dbf4/Drf1-dependent kinase Cdc7 in DNA-damage checkpoint control. (PubMed id 19111665)1, 3 Tsuji T....Jiang W. (2008)
- Inhibition of Cdc7/Dbf4 kinase activity affects specific phosphorylation sites on MCM2 in cancer cells. (PubMed id 18286467)3 Charych D.H....Walter A.O. (2008)
|
Search for DBF4
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing DBF4
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing DBF4
(According to HUGE)
About This Section
| -- |
Specialized Databases showing DBF4(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for DBF4(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for DBF4:

 | |
 | | | | |
|
 |  |  |
| | Recombinant Proteins (DBF4) | | Antibodies (DBF4) |
| | | Search Tocris compounds for DBF4 |
|
 |
 | | | |
GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009
|