V3 here soon-try beta now
Gene Search (GeneCards Home) | GeneCards Guide | User Feedback | Terms of Use | Notice about third-party sites
 

DBC1 Gene

protein-coding   GIFtS: 54

GC09M120968
deleted in bladder cancer 1
(Previous name: deleted in bladder cancer chromosome region candidate 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: DBCCR1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
DBCCR1 2, 3
FAM5A 1, 2, 3
KIAA1967 5
OTTHUMP00000022751 2
Descriptions
Protein FAM5A 3
bA574M5.1 (deleted in bladder cancer chromosome region
candidate 1 (IB3089A)) 2
deleted in bladder cancer 1 2
deleted in bladder cancer chromosome region candidate 1 1, 2
External Ids
HGNC: 26871
Entrez Gene: 16202
UniProtKB: O604773
Ensembl: ENSG000000787257
Search outside databases for aliases for DBC1 gene

Previous GC identifers: GC09U990016 GC09M117304 GC09M119008

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for DBC1:
This gene is located within a chromosomal region that shows loss of heterozygosity in some bladder
cancers. It contains a 5' CpG island that may be a frequent target of hypermethylation, and
it may undergo hypermethylation-based silencing in some bladder cancers. [provided by RefSeq]

UniProtKB/Swiss-Prot: DBC1_HUMAN, O60477
Function: Inhibits cell proliferation by negative regulation of the G1/S transition. Mediates cell
death which is not of the classical apoptotic type and regulates expression of components of the
plasminogen pathway

Gene Wiki entry for DBC1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DBC1 gene  

Entrez Gene cytogenetic band: 9q32-q33   Ensembl cytogenetic band:  9q33.1   HGNC cytogenetic band: 9q32-q33

DBC1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09M120968:     (about GC identifiers)

Start:
120,968,728 bp from pter
End:
121,171,566 bp from pter
Size:
202,839 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000009.10  NT_008470.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: DBC1_HUMAN, O60477 (See protein sequence)
Recommended Name: Deleted in bladder cancer protein 1 precursor  
Size: 761 amino acids; 88760 Da
Subcellular location: Cytoplasm
Miscellaneous: DBC1 is silenced by methylation in 50% of bladder cancer cell lines
Secondary accessions: Q6IPV6 Q6P1A0 Q8WU22
Alternative splicing: 3 isoforms:  O60477-1   O60477-2   O60477-3   

REFSEQ proteins: NP_055433.2  

ENSEMBL proteins: 
ENSP00000363080 ENSP00000363075 ENSP00000265922 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (DBC1)
Human Recombinant Proteins from Abnova (DBC1)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IDA11420708
About this table

Antibodies for DBC1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (DBC1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (DBC1)
Novus Biologicals Antibodies for DBC1

Assays for DBC1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

1 InterPro domain/family:
 IPR001862 MAC_perforin


   GeneDecks  DBC1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry O60477

ProtoNet protein and cluster: O60477

UniProtKB/Swiss-Prot: DBC1_HUMAN, O60477
Similarity: Belongs to the FAM5 family
Similarity: Contains 1 MACPF domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (DBC1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (DBC1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014618

              Applied Biosystems Silencer® siRNAs for DBC1

              Sigma-Aldrich siRNA for DBC1  
                     Sigma-Aldrich shRNA for DBC1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for DBC1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014618
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014618
                                 untagged cDNA clone in CMV expression vector: NM_014618 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_014618

UniProtKB/Swiss-Prot: DBC1_HUMAN, O60477
Function: Inhibits cell proliferation by negative regulation of the G1/S transition. Mediates cell
death which is not of the classical apoptotic type and regulates expression of components of the
plasminogen pathway

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005515 protein binding IPI12442002
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



3 Interacting proteins for DBC1 (ENSP000003630803) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
SMPDL3AENSP000003574253STRING (score=.944)
KIAA1967ENSP000003106703STRING (score=.912)
SMPD1ENSP000003404093STRING (score=.716)
About this table

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007050 cell cycle arrest IEA--
GO:0008219 cell death IDA14712213
GO:0045786 negative regulation of cell cycle IDA11420708
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for DBC1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (DBC1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (DBC1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014618

              Sigma-Aldrich siRNA for DBC1  
                     Sigma-Aldrich shRNA for DBC1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_014618  

REFSEQ mRNAs for DBC1 gene: 

NM_014618.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_014618  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014618
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014618
                                 untagged cDNA clone in CMV expression vector: NM_014618 

Additional cDNA sequence: 

AF027734.1 AK293501.1 AK293649.1 BC021560.1 BC065196.1 BC071702.1 

8 DOTS entries:

DT.113756  DT.100744735  DT.91899659  DT.92418485  DT.92418486  DT.95373446  DT.95373447  DT.99974558 

24/58 AceView cDNA sequences (see all 58 ):

BU626191 BM929506 BI488483 Z41452 F08986 BC071702 T16063 AI874202 
BM726737 AW955370 CD625836 BC021560 AI753300 NM_014618 BG828045 R42933 
BM681521 AA599898 AA948693 BF348205 BG419841 AF027734 CB047003 AA326334 

highest scoring ESTs for DBC1:

AF027734 BC021560 BC065196 BC071702 BG419841 BI755901 BI917293 BQ889616 BX441404 CD652209 

Unigene Cluster for DBC1:

Deleted in bladder cancer 1
Hs.532316  [show with all ESTs]
Unigene Representative Sequence: NM_014618


GeneLoc Exon Structure

3 Ensembl transcripts including schematic representations:
ENST00000373969  ENST00000373964  ENST00000265922  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

DBC1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for DBC1

1 / 2 / 3

4 probe-sets matching DBC1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
40292_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF027734 1.00 1.00 1.00 1

205818_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014618 0.60 1.00 0.82 1

1555457_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

205818_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  DBC1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.532316

Expression variation in blood from EXPOLDB for DBC1

UniProtKB/Swiss-Prot: DBC1_HUMAN, O60477
Tissue specificity: Highly expressed in brain. Weakly expressed in heart, lung, skeletal muscle,
kidney, thymus, prostate, testis and small intestine

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for DBC1 gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
DBC11   -- deleted in bladder cancer 1 94.35(n)
99.34(a)
481694  XM_538815.2  XP_538815.2 
chimpanzee
(Pan troglodytes)
DBC11   -- deleted in bladder cancer 1 99.39(n)
100(a)
464694  XM_520219.2  XP_520219.2 
cow
(Bos taurus)
DBC11   -- deleted in bladder cancer 1 93.82(n)
98.82(a)
538990  NM_001015669.1  NP_001015669.1 
rat
(Rattus norvegicus)
Dbc11   -- deleted in bladder cancer 1 (human) 92.15(n)
98.68(a)
140610  NM_080482.1  NP_536730.1 
mouse
(Mus musculus)
Dbc11, 5 45
deleted in bladder cancer 1 (human)1, 5 92.37(n)1
98.82(a)1
567101  NM_019967.21  NP_064351.21 
 AB0605875  AB0605895  (see all 13)
About this table        Species with no ortholog for DBC1

ENSEMBL Gene Tree for DBC1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for DBC1 gene
FAM5B2  FAM5C2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/1022 NCBI SNPs in DBC1 are shown (see all 1022 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 360)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 9 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs13246281,2
A,C,F,H,O121172145(+) AGGAGT/GGAGAA 1 -- ng31 trp35Minor allele frequency- G:0.32MN EU EA WA 602
rs13246271,2
A,C,F,H121172005(+) GGGAGG/TTGCCC 1 -- ng31 tfbs35Minor allele frequency- T:0.29MN EU EA WA 602
rs70204621,2
A,C,F,H121172926(+) GAACTG/TGGAAC 1 -- ng318Minor allele frequency- T:0.19EU EA WA 838
rs13246261,2
A,C,F,H121171959(+) GGTGTC/TTCCAC 1 -- ng315Minor allele frequency- T:0.35MN EU EA WA 596
rs174767831,2
C,F,H121010890(+) CAGTGC/TGTTGG 1 R/H mis1 ese311Minor allele frequency- T:0.01NA EU EA WA 980
rs37583381,2
C,F,H121172347(-) AACATA/CCCAAA 1 -- ng31 tfbs35Minor allele frequency- C:0.36EA EU WA 1724
rs117918771,2
C,H121171427(+) TTGCTG/CGCTCG 1 -- ut512Minor allele frequency- C:0.01EA 164
rs14737851,2
C,H121040777(+) TCTCCG/ATGCTT 1 M/T mis1 ese35Minor allele frequency- A:0.00MN EU EA WA 592
rs37583391,2
H121172182(-) CTCTGG/ATGGTC 1 -- ng314Minor allele frequency- A:0.00EU EA WA 418
rs20653981,2
C121171192(+) GCGGGA/CGGACG 1 -- ut51 ese30--------
About this table

HapMap Linkage Disequilibrium images for DBC1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607359

5 Novoseek disease relationships for DBC1 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
bladder cancer 73.34 13 11420708 (3), 9545632 (2), 14712213 (1), 12442002 (1) (see all 7)
carcinoma transitional cell 65.32 1 11921286 (1)
bladder tumors 64.45 5 14712213 (2), 12442002 (1), 11921286 (1)
tumors 27.00 10 15746151 (3), 14712213 (1), 9545632 (1), 11450846 (1) (see all 8)
cancer 0.00 1 15746151 (1)
About this table

Human Genome Epidemiology Navigator: DBC1 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/29 PubMed articles for DBC1 gene (see all 29 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 1620 HGNC: 2687 AceView: DBC1 Ensembl:ENSG00000078725 euGenes: HUgn1620
ECgene: DBC1 H-InvDB: DBC1
(According to HUGE)
About This Section

HUGE: KIAA1967
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

NameDescription
ATLAS Chromosomes in Cancer entry for DBC1 Genetics and Cytogenetics in Oncology and Haematology
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for DBC1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for DBC1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for DBC1 Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for DBC1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
 Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
 Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
 Recombinant & Natural Proteins  

 Recombinant Proteins (DBC1)
 Antibodies (DBC1)
 Chimera RNAi (DBC1)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(DBC1)
 Antibodies (DBC1)
 Search OriGene for DBC1
 Search Tocris compounds for DBC1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for DBC1

GeneCards Homepage    -    Last full update: 2 Jul 2009        Incremental update: 13 Oct 2009

Random Gene From GIFtS : about GIFtS

Gene Index:   3   5   A   B   C   D   E   F   G   H   I   J   K   L   M   N   O   P   Q   R   S   T   U   V   W   X   Y   Z

Developed at the Crown Human Genome Center & Weizmann Institute of Science



The GeneCards Human Gene Database: Copyright © 1996-2009, Weizmann Institute of Science. All Rights Reserved.
DBC1 Gene at Home site.
Version 2.41.1
server4.xennexinc.com