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DAZAP2 Gene

protein-coding   GIFtS: 54

GC12P049918
DAZ associated protein 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
KIAA0058 1, 2, 3
MGC14319 2
MGC766 2
PRTB 2
Descriptions
DAZ associated protein 2 2
Deleted in azoospermia-associated protein 2 3
deleted in azoospermia associated protein 2 2
proline-rich transcript in brain 2
proline-rich transcript, brain-expressed protein 2
External Ids
HGNC: 26841
Entrez Gene: 98022
UniProtKB: Q150383
Ensembl: ENSG000001832837
Search outside databases for aliases for DAZAP2 gene

Previous GC identifers: GC02U990100 GC12P051349

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for DAZAP2:
This gene encodes a proline-rich protein which interacts with the deleted in azoospermia (DAZ) and
the deleted in azoospermia-like gene through the DAZ-like repeats. This protein also interacts
with the transforming growth factor-beta signaling molecule SARA (Smad anchor for receptor
activation), eukaryotic initiation factor 4G, and an E3 ubiquitinase that regulates its stability
in splicing factor containing nuclear speckles. The encoded protein may function in various
biological and pathological processes including spermatogenesis, cell signaling and transcription
regulation, formation of stress granules during translation arrest, RNA splicing, and pathogenesis
of multiple myeloma. Multiple transcript variants encoding different isoforms have been found for
this gene. [provided by RefSeq]

Gene Wiki entry for DAZAP2
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the DAZAP2 gene  

Entrez Gene cytogenetic band: 12q12   Ensembl cytogenetic band:  12q13.13   HGNC cytogenetic band: 12q12

DAZAP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P049918:     (about GC identifiers)

Start:
49,918,877 bp from pter
End:
49,923,834 bp from pter
Size:
4,958 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_029419.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: DAZP2_HUMAN, Q15038 (See protein sequence)
Recommended Name: DAZ-associated protein 2  
Size: 168 amino acids; 17319 Da
Subunit: Interacts with SOX6 (By similarity). Interacts with DAZ and DAZL

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (6 alternative transcripts): 
    NP_001129736.1  NP_001129738.1  NP_001129739.1  NP_001129740.1  NP_001129741.1  NP_055579.1  


    ENSEMBL proteins: 
    ENSP00000331100 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
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    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (DAZAP2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for DAZAP2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Monoclonal and Polyclonal Antibodies from Abnova (DAZAP2)
    Novus Biologicals Antibody for DAZAP2

    Assays for DAZAP2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ProtoNet protein and cluster: Q15038

    1 Blocks protein family: IPB000515 Binding-protein-dependent transport systems inner membrane component

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAZAP2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DAZAP2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 6): NM_001136266

                  Applied Biosystems Silencer® siRNAs for DAZAP2

                  Sigma-Aldrich siRNA for DAZAP2  
                         Sigma-Aldrich shRNA for DAZAP2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for DAZAP2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 6): NM_001136264
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 6): NM_001136264
                                     untagged cDNA clones in CMV expression vector (see all 6): NM_001136264 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_001136266

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Dazap2):

    normal

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IPI16713569
    GO:0050699 WW domain binding IPI11342538
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  DAZAP2 


    5/39 Interacting proteins for DAZAP2 (Q150381, 2 ENSP000003311003) via UniProtKB, MINT, and/or STRING (see all 39 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ATXN1P542531, 2EBI-724310, EBI-930964 MINT-2855146 MINT-2867596 EBI-724310, EBI-930964 MINT-2855146 MINT-2867596
    ATXN1P542531, 2EBI-724310, EBI-930964 MINT-2855146 MINT-2867596 EBI-724310, EBI-930964 MINT-2855146 MINT-2867596
    AATFQ9NY612MINT-66876
    CALCOCO2Q131372MINT-68098
    DAZAP2Q150382MINT-66170
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for DAZAP2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (DAZAP2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (DAZAP2)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 6): NM_001136266

                  Sigma-Aldrich siRNA for DAZAP2  
                         Sigma-Aldrich shRNA for DAZAP2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001136264  NM_001136266  NM_001136267  NM_001136268  NM_001136269  NM_014764  

    REFSEQ mRNAs for DAZAP2 gene (6 alternative transcripts): 

    NM_001136264.1   NM_001136266.1   NM_001136267.1   NM_001136268.1   NM_001136269.1   NM_014764.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001136264  NM_001136266  NM_001136267  NM_001136269  NM_014764  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 6): NM_001136264
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 6): NM_001136264
                                     untagged cDNA clones in CMV expression vector (see all 6): NM_001136264 

    Additional cDNA sequence: 

    AK095963.1 AK125855.1 AK290119.1 AK293330.1 AK303828.1 AY430097.1 BC002334.2 BC007900.2 
    BX647278.1 CR590843.1 CR594491.1 CR595995.1 CR598969.1 CR599409.1 CR602531.1 CR607711.1 
    CR609119.1 CR612770.1 CR614908.1 D31767.1 

    24/52 DOTS entries (see all 52 ):

    DT.95120036  DT.92344543  DT.454301  DT.91781467  DT.100881025  DT.100881017  DT.95120028  DT.100783065 
    DT.87014327  DT.91802986  DT.100881030  DT.91797300  DT.95120057  DT.100637838  DT.305912  DT.121124887 
    DT.100881010  DT.205570  DT.121124803  DT.121124691  DT.120976765  DT.97760188  DT.121125049  DT.95230545 

    24/1283 AceView cDNA sequences (see all 1283 ):

    AA367230 AA429968 AI475369 AA314671 BG545831 AI471961 BM970053 AA653092 
    AA346880 AI057417 AA973661 N43920 BF795191 BM710623 AA635666 AI250444 
    BM927415 AW518078 CR614908 AA737611 CF593495 BI711817 BM766493 BU169827 

    highest scoring ESTs for DAZAP2:

    D31767 AA009590 AA025019 AA028897 AA028898 AA033735 AA035050 AA035291 AA035327 AA039878 

    Unigene Cluster for DAZAP2:

    DAZ associated protein 2
    Hs.369761  [show with all ESTs]
    Unigene Representative Sequence: AK125855


    GeneLoc Exon Structure

    5 Alternative Splicing Database (ASD) splice patterns (SP) for DAZAP2

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6
    SP1:                                -                                 
    SP2:                                                                  
    SP3:                                -                 -     -         
    SP4:                                -     -     -                     
    SP5:                                                                  

    About this scheme

    ECgene alternative splicing isoforms for DAZAP2

    1 Ensembl transcript including schematic representation:
    ENST00000332845  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    DAZAP2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for DAZAP2

    1 / 2 / 3

    10 probe-sets matching DAZAP2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    84890_at2, 3 U95-D 1 1.00 1.00 0.68 0.83 AI191804 0.80 1.00 0.91 1

    86494_at2, 3 U95-E 1 1.00 1.00 0.27 0.62 AI761972 0.60 1.00 0.82 1

    36616_at2, 3 U95-A 2 1.00 0.94 0.54 1.38 D31767 1.00 1.00 1.00 1

    69121_at2, 3 U95-D 2 0.50 0.88 0.54 1.19 AI697834 0.40 0.67 0.55 1

    214334_x_at2, 3 U133-A 1 1.00 1.00 -- -- N34846 0.60 1.00 0.82 1

    200794_x_at2, 3 U133-A 2 1.00 0.86 -- -- NM_014764 0.60 1.00 0.82 1

    212595_s_at2, 3 U133-A 3 1.00 0.76 -- -- AL534321 0.80 1.00 0.91 1

    214334_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    200794_x_at2 U133Plus2 2 1.00 0.86 -- -- -- -- -- -- --

    212595_s_at2 U133Plus2 3 1.00 0.76 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGATGTTTGA

    SOURCE GeneReport for Unigene cluster: Hs.369761

    Expression variation in blood from EXPOLDB for DAZAP2

    UniProtKB/Swiss-Prot: DAZP2_HUMAN, Q15038
    Tissue specificity: Widely expressed. Expressed in spleen, thymus, protate, testis, ovary, small
    intestine, colon and leukocytes. Down-regulated in multiple myeloma

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for DAZAP2 gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    DAZAP21   -- DAZ associated protein 2 95.04(n)
    99.4(a)
    477605  XM_855817.1  XP_860910.1 
    chimpanzee
    (Pan troglodytes)
    DAZAP21   -- DAZ associated protein 2 100(n)
    100(a)
    451902  XM_509065.2  XP_509065.1 
    cow
    (Bos taurus)
    DAZAP21   -- DAZ associated protein 2 95.04(n)
    100(a)
    540286  NM_001034701.1  NP_001029873.1 
    mouse
    (Mus musculus)
    Dazap21, 5 15 (60.00 cM)5
    DAZ associated protein 21, 5 93.85(n)1
    99.4(a)1
    239941  NM_011873.21  NP_036003.21 
     AF0853485  AI3147275  (see all 20)
    zebrafish
    (Danio rerio)
    wufi04d042   -- wufi04d04 73.25(n) 327356  BC063229.1 
    About this table        Species with no ortholog for DAZAP2

    ENSEMBL Gene Tree for DAZAP2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Pseudogenes
    1 related pseudogene


    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/56 NCBI SNPs in DAZAP2 are shown (see all 56 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 21)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs11306411,2
    A,C,F,H49923508(+) GGGTTA/GATAGG 1 -- ut31 ese39Minor allele frequency- N:0.01MN EU EA WA NA 792
    rs79793851,2
    A,C,F,H49918520(+) AGGCAG/ATGCGA 1 -- ng518Minor allele frequency- A:0.05EU EA WA 802
    rs64211711,2
    A,C,F,H49918531(+) CAAATT/CTGCAG 1 -- ng518Minor allele frequency- C:0.41MN EA EU WA 1844
    rs10494671,2
    A,C,F49922526(+) CACCTT/CTGTGC 1 -- ut31 ese31Minor allele frequency- C:0.16MN 184
    --
    rs107358331,2
    A,C49917283(+) GGTGGA/CAAGAT 1 -- ng510--------
    --
    rs11305981,2
    C,F49923171(+) CAGATC/GCCATT 1 -- ut311Minor allele frequency- G:0.39MN 184
    --
    rs108761561,2
    A,C49923436(+) AAAAAC/TTGGTT 1 -- ut31 ese30--------
    --
    rs73109471,2
    A,C49917813(+) ctgggC/Ggacag 1 -- ng510--------
    rs38882231,2
    A,C49918722(+) GGGGGG/TGGAGG 1 -- ng51 tfbs3 trp30--------
    --
    rs73142351,2
    C49918048(+) atcgcC/Gccact 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for DAZAP2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 607431

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/15 PubMed articles for DAZAP2 gene (see all 15 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 9802 HGNC: 2684 AceView: DAZAP2 Ensembl:ENSG00000183283 euGenes: HUgn9802
    ECgene: DAZAP2 H-InvDB: DAZAP2
    (According to HUGE)
    About This Section

    HUGE: KIAA0058
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for DAZAP2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for DAZAP2 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for DAZAP2 Browse small molecules at Sigma-Aldrich
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